Amino acid dipepetide frequency for Bacillus phage 1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.421AlaAla: 6.421 ± 1.08
0.378AlaCys: 0.378 ± 0.19
3.777AlaAsp: 3.777 ± 0.512
4.721AlaGlu: 4.721 ± 0.572
3.588AlaPhe: 3.588 ± 0.681
5.194AlaGly: 5.194 ± 0.997
0.944AlaHis: 0.944 ± 0.358
6.61AlaIle: 6.61 ± 1.191
5.194AlaLys: 5.194 ± 0.719
6.893AlaLeu: 6.893 ± 1.251
2.077AlaMet: 2.077 ± 0.464
3.494AlaAsn: 3.494 ± 0.618
1.322AlaPro: 1.322 ± 0.292
2.927AlaGln: 2.927 ± 0.593
2.833AlaArg: 2.833 ± 0.661
3.588AlaSer: 3.588 ± 0.792
3.872AlaThr: 3.872 ± 0.51
4.816AlaVal: 4.816 ± 0.968
0.755AlaTrp: 0.755 ± 0.387
3.683AlaTyr: 3.683 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.567CysAla: 0.567 ± 0.214
0.094CysCys: 0.094 ± 0.086
0.378CysAsp: 0.378 ± 0.192
0.944CysGlu: 0.944 ± 0.314
0.283CysPhe: 0.283 ± 0.155
0.472CysGly: 0.472 ± 0.18
0.094CysHis: 0.094 ± 0.091
0.378CysIle: 0.378 ± 0.187
1.416CysLys: 1.416 ± 0.359
0.85CysLeu: 0.85 ± 0.267
0.283CysMet: 0.283 ± 0.156
0.189CysAsn: 0.189 ± 0.137
0.567CysPro: 0.567 ± 0.267
0.378CysGln: 0.378 ± 0.166
0.85CysArg: 0.85 ± 0.363
0.378CysSer: 0.378 ± 0.201
0.283CysThr: 0.283 ± 0.201
0.283CysVal: 0.283 ± 0.159
0.094CysTrp: 0.094 ± 0.09
0.567CysTyr: 0.567 ± 0.263
0.0CysXaa: 0.0 ± 0.0
Asp
4.721AspAla: 4.721 ± 0.701
0.755AspCys: 0.755 ± 0.302
3.116AspAsp: 3.116 ± 0.595
7.082AspGlu: 7.082 ± 0.671
2.55AspPhe: 2.55 ± 0.518
4.438AspGly: 4.438 ± 0.545
0.85AspHis: 0.85 ± 0.294
4.155AspIle: 4.155 ± 0.626
3.305AspLys: 3.305 ± 0.578
4.06AspLeu: 4.06 ± 0.593
1.322AspMet: 1.322 ± 0.41
2.172AspAsn: 2.172 ± 0.37
1.322AspPro: 1.322 ± 0.271
1.983AspGln: 1.983 ± 0.349
3.683AspArg: 3.683 ± 0.738
2.172AspSer: 2.172 ± 0.547
2.55AspThr: 2.55 ± 0.519
4.06AspVal: 4.06 ± 0.609
0.661AspTrp: 0.661 ± 0.253
1.794AspTyr: 1.794 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
6.043GluAla: 6.043 ± 0.588
0.661GluCys: 0.661 ± 0.267
4.155GluAsp: 4.155 ± 0.712
4.627GluGlu: 4.627 ± 0.643
4.249GluPhe: 4.249 ± 0.661
3.116GluGly: 3.116 ± 0.626
1.416GluHis: 1.416 ± 0.431
6.516GluIle: 6.516 ± 0.871
6.704GluLys: 6.704 ± 0.716
10.67GluLeu: 10.67 ± 1.39
1.794GluMet: 1.794 ± 0.386
3.211GluAsn: 3.211 ± 0.692
2.077GluPro: 2.077 ± 0.402
3.966GluGln: 3.966 ± 0.623
5.76GluArg: 5.76 ± 0.649
2.455GluSer: 2.455 ± 0.441
3.777GluThr: 3.777 ± 0.61
5.005GluVal: 5.005 ± 0.679
1.228GluTrp: 1.228 ± 0.342
4.06GluTyr: 4.06 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.55PheAla: 2.55 ± 0.386
0.283PheCys: 0.283 ± 0.143
3.494PheAsp: 3.494 ± 0.722
3.399PheGlu: 3.399 ± 0.556
1.7PhePhe: 1.7 ± 0.367
2.644PheGly: 2.644 ± 0.447
0.472PheHis: 0.472 ± 0.223
3.399PheIle: 3.399 ± 0.606
3.399PheLys: 3.399 ± 0.553
3.211PheLeu: 3.211 ± 0.637
0.944PheMet: 0.944 ± 0.281
1.983PheAsn: 1.983 ± 0.44
1.983PhePro: 1.983 ± 0.356
0.85PheGln: 0.85 ± 0.276
2.833PheArg: 2.833 ± 0.443
2.738PheSer: 2.738 ± 0.484
1.322PheThr: 1.322 ± 0.308
3.022PheVal: 3.022 ± 0.517
0.567PheTrp: 0.567 ± 0.221
2.077PheTyr: 2.077 ± 0.489
0.0PheXaa: 0.0 ± 0.0
Gly
4.06GlyAla: 4.06 ± 0.71
0.567GlyCys: 0.567 ± 0.227
3.116GlyAsp: 3.116 ± 0.645
4.816GlyGlu: 4.816 ± 0.631
2.833GlyPhe: 2.833 ± 0.49
4.816GlyGly: 4.816 ± 0.618
1.228GlyHis: 1.228 ± 0.437
4.533GlyIle: 4.533 ± 0.872
5.099GlyLys: 5.099 ± 0.542
5.477GlyLeu: 5.477 ± 0.818
1.7GlyMet: 1.7 ± 0.374
2.55GlyAsn: 2.55 ± 0.401
0.755GlyPro: 0.755 ± 0.315
1.889GlyGln: 1.889 ± 0.481
2.738GlyArg: 2.738 ± 0.501
2.927GlySer: 2.927 ± 0.697
3.683GlyThr: 3.683 ± 0.589
3.399GlyVal: 3.399 ± 0.51
0.755GlyTrp: 0.755 ± 0.318
3.494GlyTyr: 3.494 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
1.039HisAla: 1.039 ± 0.333
0.472HisCys: 0.472 ± 0.252
1.228HisAsp: 1.228 ± 0.309
1.7HisGlu: 1.7 ± 0.414
0.661HisPhe: 0.661 ± 0.293
0.85HisGly: 0.85 ± 0.34
0.661HisHis: 0.661 ± 0.33
1.133HisIle: 1.133 ± 0.383
1.605HisLys: 1.605 ± 0.368
1.7HisLeu: 1.7 ± 0.425
0.189HisMet: 0.189 ± 0.128
0.661HisAsn: 0.661 ± 0.262
0.755HisPro: 0.755 ± 0.26
0.661HisGln: 0.661 ± 0.251
0.85HisArg: 0.85 ± 0.259
0.85HisSer: 0.85 ± 0.296
0.85HisThr: 0.85 ± 0.389
1.322HisVal: 1.322 ± 0.305
0.0HisTrp: 0.0 ± 0.0
0.944HisTyr: 0.944 ± 0.317
0.0HisXaa: 0.0 ± 0.0
Ile
5.76IleAla: 5.76 ± 0.612
0.661IleCys: 0.661 ± 0.263
4.438IleAsp: 4.438 ± 0.548
7.365IleGlu: 7.365 ± 0.731
2.172IlePhe: 2.172 ± 0.504
4.155IleGly: 4.155 ± 0.671
2.077IleHis: 2.077 ± 0.492
4.627IleIle: 4.627 ± 0.724
5.477IleLys: 5.477 ± 0.857
4.155IleLeu: 4.155 ± 0.528
1.133IleMet: 1.133 ± 0.341
2.927IleAsn: 2.927 ± 0.514
3.683IlePro: 3.683 ± 0.588
2.361IleGln: 2.361 ± 0.524
5.288IleArg: 5.288 ± 0.54
4.91IleSer: 4.91 ± 0.848
4.06IleThr: 4.06 ± 0.545
4.344IleVal: 4.344 ± 0.692
0.661IleTrp: 0.661 ± 0.214
2.361IleTyr: 2.361 ± 0.492
0.0IleXaa: 0.0 ± 0.0
Lys
5.76LysAla: 5.76 ± 1.135
0.85LysCys: 0.85 ± 0.33
3.966LysAsp: 3.966 ± 0.681
6.516LysGlu: 6.516 ± 0.741
2.361LysPhe: 2.361 ± 0.417
4.627LysGly: 4.627 ± 0.604
1.605LysHis: 1.605 ± 0.4
6.043LysIle: 6.043 ± 0.747
6.516LysLys: 6.516 ± 0.887
6.043LysLeu: 6.043 ± 0.816
1.889LysMet: 1.889 ± 0.427
4.155LysAsn: 4.155 ± 0.597
2.266LysPro: 2.266 ± 0.552
3.777LysGln: 3.777 ± 0.554
4.627LysArg: 4.627 ± 0.775
4.155LysSer: 4.155 ± 0.782
5.288LysThr: 5.288 ± 0.652
4.533LysVal: 4.533 ± 0.719
1.228LysTrp: 1.228 ± 0.34
2.55LysTyr: 2.55 ± 0.553
0.0LysXaa: 0.0 ± 0.0
Leu
7.177LeuAla: 7.177 ± 0.876
0.755LeuCys: 0.755 ± 0.264
5.855LeuAsp: 5.855 ± 0.683
6.988LeuGlu: 6.988 ± 0.99
2.833LeuPhe: 2.833 ± 0.462
5.855LeuGly: 5.855 ± 0.779
1.511LeuHis: 1.511 ± 0.396
5.194LeuIle: 5.194 ± 0.673
6.893LeuLys: 6.893 ± 0.847
8.404LeuLeu: 8.404 ± 0.864
2.644LeuMet: 2.644 ± 0.541
4.438LeuAsn: 4.438 ± 0.867
2.455LeuPro: 2.455 ± 0.442
3.305LeuGln: 3.305 ± 0.491
4.155LeuArg: 4.155 ± 0.71
5.666LeuSer: 5.666 ± 0.956
3.966LeuThr: 3.966 ± 0.67
4.627LeuVal: 4.627 ± 0.69
0.567LeuTrp: 0.567 ± 0.241
3.211LeuTyr: 3.211 ± 0.511
0.0LeuXaa: 0.0 ± 0.0
Met
2.172MetAla: 2.172 ± 0.427
0.378MetCys: 0.378 ± 0.199
0.944MetAsp: 0.944 ± 0.34
2.172MetGlu: 2.172 ± 0.517
1.133MetPhe: 1.133 ± 0.395
1.511MetGly: 1.511 ± 0.312
0.378MetHis: 0.378 ± 0.18
1.983MetIle: 1.983 ± 0.369
2.738MetLys: 2.738 ± 0.418
1.983MetLeu: 1.983 ± 0.434
0.283MetMet: 0.283 ± 0.162
1.133MetAsn: 1.133 ± 0.283
1.133MetPro: 1.133 ± 0.341
0.755MetGln: 0.755 ± 0.295
1.605MetArg: 1.605 ± 0.407
1.133MetSer: 1.133 ± 0.269
2.266MetThr: 2.266 ± 0.451
1.228MetVal: 1.228 ± 0.305
0.378MetTrp: 0.378 ± 0.166
1.133MetTyr: 1.133 ± 0.349
0.0MetXaa: 0.0 ± 0.0
Asn
3.777AsnAla: 3.777 ± 1.336
0.283AsnCys: 0.283 ± 0.158
2.927AsnAsp: 2.927 ± 0.544
3.022AsnGlu: 3.022 ± 0.531
1.7AsnPhe: 1.7 ± 0.399
3.683AsnGly: 3.683 ± 0.649
0.567AsnHis: 0.567 ± 0.291
3.777AsnIle: 3.777 ± 0.533
2.55AsnLys: 2.55 ± 0.506
3.683AsnLeu: 3.683 ± 0.595
1.605AsnMet: 1.605 ± 0.303
1.889AsnAsn: 1.889 ± 0.622
2.927AsnPro: 2.927 ± 0.612
1.322AsnGln: 1.322 ± 0.466
1.416AsnArg: 1.416 ± 0.376
2.644AsnSer: 2.644 ± 0.459
2.927AsnThr: 2.927 ± 0.521
3.022AsnVal: 3.022 ± 0.643
0.378AsnTrp: 0.378 ± 0.166
1.7AsnTyr: 1.7 ± 0.527
0.0AsnXaa: 0.0 ± 0.0
Pro
1.794ProAla: 1.794 ± 0.368
0.094ProCys: 0.094 ± 0.108
2.266ProAsp: 2.266 ± 0.372
3.211ProGlu: 3.211 ± 0.475
1.889ProPhe: 1.889 ± 0.408
2.361ProGly: 2.361 ± 0.483
0.85ProHis: 0.85 ± 0.322
2.644ProIle: 2.644 ± 0.462
2.55ProLys: 2.55 ± 0.456
2.455ProLeu: 2.455 ± 0.388
0.567ProMet: 0.567 ± 0.21
0.85ProAsn: 0.85 ± 0.256
1.039ProPro: 1.039 ± 0.302
0.85ProGln: 0.85 ± 0.236
1.889ProArg: 1.889 ± 0.379
1.794ProSer: 1.794 ± 0.452
1.605ProThr: 1.605 ± 0.366
1.889ProVal: 1.889 ± 0.366
0.567ProTrp: 0.567 ± 0.24
1.889ProTyr: 1.889 ± 0.6
0.0ProXaa: 0.0 ± 0.0
Gln
3.305GlnAla: 3.305 ± 0.802
0.567GlnCys: 0.567 ± 0.209
1.133GlnAsp: 1.133 ± 0.26
3.211GlnGlu: 3.211 ± 0.466
1.511GlnPhe: 1.511 ± 0.396
1.605GlnGly: 1.605 ± 0.506
0.567GlnHis: 0.567 ± 0.214
2.266GlnIle: 2.266 ± 0.497
3.494GlnLys: 3.494 ± 0.527
2.644GlnLeu: 2.644 ± 0.742
1.228GlnMet: 1.228 ± 0.348
2.266GlnAsn: 2.266 ± 0.462
0.944GlnPro: 0.944 ± 0.263
1.794GlnGln: 1.794 ± 0.396
2.172GlnArg: 2.172 ± 0.358
1.228GlnSer: 1.228 ± 0.32
2.077GlnThr: 2.077 ± 0.472
3.022GlnVal: 3.022 ± 0.509
0.755GlnTrp: 0.755 ± 0.315
1.511GlnTyr: 1.511 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
3.494ArgAla: 3.494 ± 0.551
0.755ArgCys: 0.755 ± 0.358
3.022ArgAsp: 3.022 ± 0.412
4.155ArgGlu: 4.155 ± 0.665
3.022ArgPhe: 3.022 ± 0.506
3.022ArgGly: 3.022 ± 0.479
1.133ArgHis: 1.133 ± 0.33
4.06ArgIle: 4.06 ± 0.734
5.76ArgLys: 5.76 ± 0.837
4.627ArgLeu: 4.627 ± 0.655
2.266ArgMet: 2.266 ± 0.428
2.455ArgAsn: 2.455 ± 0.496
2.077ArgPro: 2.077 ± 0.444
2.455ArgGln: 2.455 ± 0.473
3.683ArgArg: 3.683 ± 0.683
2.833ArgSer: 2.833 ± 0.527
2.738ArgThr: 2.738 ± 0.448
3.399ArgVal: 3.399 ± 0.664
0.567ArgTrp: 0.567 ± 0.241
1.794ArgTyr: 1.794 ± 0.469
0.0ArgXaa: 0.0 ± 0.0
Ser
2.455SerAla: 2.455 ± 0.53
0.283SerCys: 0.283 ± 0.169
3.777SerAsp: 3.777 ± 0.732
4.06SerGlu: 4.06 ± 0.863
3.022SerPhe: 3.022 ± 0.444
2.172SerGly: 2.172 ± 0.473
0.755SerHis: 0.755 ± 0.28
3.588SerIle: 3.588 ± 0.607
3.777SerLys: 3.777 ± 0.753
6.232SerLeu: 6.232 ± 0.751
1.322SerMet: 1.322 ± 0.327
2.455SerAsn: 2.455 ± 0.44
1.511SerPro: 1.511 ± 0.363
1.7SerGln: 1.7 ± 0.569
3.022SerArg: 3.022 ± 0.645
3.399SerSer: 3.399 ± 0.807
2.738SerThr: 2.738 ± 0.449
3.683SerVal: 3.683 ± 0.739
0.944SerTrp: 0.944 ± 0.241
1.039SerTyr: 1.039 ± 0.549
0.0SerXaa: 0.0 ± 0.0
Thr
4.249ThrAla: 4.249 ± 0.795
0.189ThrCys: 0.189 ± 0.143
2.172ThrAsp: 2.172 ± 0.42
4.06ThrGlu: 4.06 ± 0.576
1.605ThrPhe: 1.605 ± 0.396
3.494ThrGly: 3.494 ± 0.54
0.85ThrHis: 0.85 ± 0.209
4.249ThrIle: 4.249 ± 0.615
3.116ThrLys: 3.116 ± 0.61
4.438ThrLeu: 4.438 ± 0.789
1.228ThrMet: 1.228 ± 0.271
3.872ThrAsn: 3.872 ± 0.571
2.077ThrPro: 2.077 ± 0.474
2.077ThrGln: 2.077 ± 0.384
2.55ThrArg: 2.55 ± 0.557
3.116ThrSer: 3.116 ± 0.586
3.116ThrThr: 3.116 ± 0.588
3.683ThrVal: 3.683 ± 0.642
0.472ThrTrp: 0.472 ± 0.179
2.172ThrTyr: 2.172 ± 0.527
0.0ThrXaa: 0.0 ± 0.0
Val
4.627ValAla: 4.627 ± 0.677
0.472ValCys: 0.472 ± 0.205
3.683ValAsp: 3.683 ± 0.512
5.571ValGlu: 5.571 ± 0.725
2.927ValPhe: 2.927 ± 0.557
3.683ValGly: 3.683 ± 0.502
1.322ValHis: 1.322 ± 0.409
4.533ValIle: 4.533 ± 0.667
5.194ValLys: 5.194 ± 0.631
5.382ValLeu: 5.382 ± 0.744
1.983ValMet: 1.983 ± 0.364
2.833ValAsn: 2.833 ± 0.555
2.266ValPro: 2.266 ± 0.473
2.266ValGln: 2.266 ± 0.492
3.777ValArg: 3.777 ± 0.573
3.022ValSer: 3.022 ± 0.584
2.738ValThr: 2.738 ± 0.425
3.588ValVal: 3.588 ± 0.64
0.85ValTrp: 0.85 ± 0.347
2.361ValTyr: 2.361 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
0.85TrpAla: 0.85 ± 0.241
0.094TrpCys: 0.094 ± 0.086
0.567TrpAsp: 0.567 ± 0.226
0.944TrpGlu: 0.944 ± 0.332
0.567TrpPhe: 0.567 ± 0.28
0.378TrpGly: 0.378 ± 0.166
0.094TrpHis: 0.094 ± 0.094
0.472TrpIle: 0.472 ± 0.206
0.661TrpLys: 0.661 ± 0.245
0.85TrpLeu: 0.85 ± 0.27
0.661TrpMet: 0.661 ± 0.221
0.85TrpAsn: 0.85 ± 0.705
0.378TrpPro: 0.378 ± 0.198
0.567TrpGln: 0.567 ± 0.204
0.85TrpArg: 0.85 ± 0.276
0.378TrpSer: 0.378 ± 0.17
0.661TrpThr: 0.661 ± 0.274
1.133TrpVal: 1.133 ± 0.314
0.189TrpTrp: 0.189 ± 0.148
0.755TrpTyr: 0.755 ± 0.266
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.266TyrAla: 2.266 ± 0.537
0.755TyrCys: 0.755 ± 0.324
2.738TyrAsp: 2.738 ± 0.593
2.927TyrGlu: 2.927 ± 0.702
2.266TyrPhe: 2.266 ± 0.507
2.455TyrGly: 2.455 ± 0.487
0.755TyrHis: 0.755 ± 0.271
2.455TyrIle: 2.455 ± 0.449
3.305TyrLys: 3.305 ± 0.762
2.738TyrLeu: 2.738 ± 0.489
1.228TyrMet: 1.228 ± 0.405
1.416TyrAsn: 1.416 ± 0.391
1.511TyrPro: 1.511 ± 0.405
1.416TyrGln: 1.416 ± 0.375
2.738TyrArg: 2.738 ± 0.515
2.55TyrSer: 2.55 ± 0.51
2.266TyrThr: 2.266 ± 0.45
3.022TyrVal: 3.022 ± 0.647
0.283TyrTrp: 0.283 ± 0.164
1.322TyrTyr: 1.322 ± 0.422
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (10591 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski