Freshwater phage uvFW-CGR-AMD-COM-C203

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XTK9|A0A1B0XTK9_9CAUD Adenylyl-sulfate kinase OS=Freshwater phage uvFW-CGR-AMD-COM-C203 OX=1838151 GN=uvFWCGRAMDCOMC203_042 PE=4 SV=1
MM1 pKa = 7.92PGTTTKK7 pKa = 10.83GLRR10 pKa = 11.84YY11 pKa = 7.28PTAGDD16 pKa = 3.33NPAVHH21 pKa = 6.72TDD23 pKa = 3.03IFNLATDD30 pKa = 4.15TDD32 pKa = 4.33TEE34 pKa = 4.51LNDD37 pKa = 3.75YY38 pKa = 8.01LTTAIAATTYY48 pKa = 11.47ASIANSATIYY58 pKa = 10.55EE59 pKa = 4.47VVTTGFLLGGMM70 pKa = 4.61

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XTK6|A0A1B0XTK6_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C203 OX=1838151 GN=uvFWCGRAMDCOMC203_046 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 9.31VTKK5 pKa = 10.25SRR7 pKa = 11.84SFTKK11 pKa = 10.26SVSYY15 pKa = 10.63RR16 pKa = 11.84IFGTLSSFAVVFIITGEE33 pKa = 4.26GTLSAIIAFWEE44 pKa = 4.47TVVKK48 pKa = 10.64VGIYY52 pKa = 8.78YY53 pKa = 8.28WHH55 pKa = 6.04EE56 pKa = 4.02RR57 pKa = 11.84VWNRR61 pKa = 11.84IKK63 pKa = 10.34WGRR66 pKa = 11.84VQQ68 pKa = 4.12

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

17096

32

1147

194.3

21.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.505 ± 0.366

0.632 ± 0.088

5.902 ± 0.287

6.926 ± 0.413

3.609 ± 0.239

7.61 ± 0.551

1.456 ± 0.168

6.23 ± 0.292

5.92 ± 0.355

7.259 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.135

4.223 ± 0.176

4.527 ± 0.824

3.457 ± 0.156

4.381 ± 0.288

6.914 ± 0.32

7.598 ± 0.501

7.335 ± 0.278

1.544 ± 0.167

2.948 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski