Amino acid dipepetide frequency for Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.879AlaAla: 13.879 ± 0.198	1.172AlaCys: 1.172 ± 0.042	5.63AlaAsp: 5.63 ± 0.097	6.831AlaGlu: 6.831 ± 0.102	3.514AlaPhe: 3.514 ± 0.077	8.62AlaGly: 8.62 ± 0.125	2.691AlaHis: 2.691 ± 0.056	6.159AlaIle: 6.159 ± 0.092	3.391AlaLys: 3.391 ± 0.081	13.023AlaLeu: 13.023 ± 0.148	3.166AlaMet: 3.166 ± 0.059	2.841AlaAsn: 2.841 ± 0.056	4.602AlaPro: 4.602 ± 0.087	5.078AlaGln: 5.078 ± 0.1	7.019AlaArg: 7.019 ± 0.118	5.213AlaSer: 5.213 ± 0.093	5.353AlaThr: 5.353 ± 0.084	7.999AlaVal: 7.999 ± 0.105	1.689AlaTrp: 1.689 ± 0.049	2.772AlaTyr: 2.772 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.153CysAla: 1.153 ± 0.039	0.131CysCys: 0.131 ± 0.015	0.455CysAsp: 0.455 ± 0.023	0.457CysGlu: 0.457 ± 0.025	0.352CysPhe: 0.352 ± 0.02	0.968CysGly: 0.968 ± 0.036	0.33CysHis: 0.33 ± 0.019	0.467CysIle: 0.467 ± 0.027	0.321CysLys: 0.321 ± 0.024	0.901CysLeu: 0.901 ± 0.033	0.25CysMet: 0.25 ± 0.019	0.284CysAsn: 0.284 ± 0.018	0.619CysPro: 0.619 ± 0.036	0.368CysGln: 0.368 ± 0.022	0.698CysArg: 0.698 ± 0.034	0.519CysSer: 0.519 ± 0.03	0.469CysThr: 0.469 ± 0.027	0.609CysVal: 0.609 ± 0.027	0.124CysTrp: 0.124 ± 0.012	0.237CysTyr: 0.237 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.799AspAla: 5.799 ± 0.099	0.53AspCys: 0.53 ± 0.029	2.487AspAsp: 2.487 ± 0.062	2.712AspGlu: 2.712 ± 0.069	2.297AspPhe: 2.297 ± 0.062	4.0AspGly: 4.0 ± 0.075	1.435AspHis: 1.435 ± 0.05	2.882AspIle: 2.882 ± 0.06	1.639AspLys: 1.639 ± 0.049	5.872AspLeu: 5.872 ± 0.097	1.37AspMet: 1.37 ± 0.044	1.3AspAsn: 1.3 ± 0.042	2.946AspPro: 2.946 ± 0.062	1.896AspGln: 1.896 ± 0.05	3.402AspArg: 3.402 ± 0.064	2.377AspSer: 2.377 ± 0.06	2.427AspThr: 2.427 ± 0.051	3.374AspVal: 3.374 ± 0.068	1.04AspTrp: 1.04 ± 0.033	1.556AspTyr: 1.556 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.054GluAla: 6.054 ± 0.106	0.429GluCys: 0.429 ± 0.026	2.916GluAsp: 2.916 ± 0.062	3.308GluGlu: 3.308 ± 0.076	1.577GluPhe: 1.577 ± 0.052	3.948GluGly: 3.948 ± 0.08	1.455GluHis: 1.455 ± 0.044	3.631GluIle: 3.631 ± 0.077	2.432GluLys: 2.432 ± 0.061	5.114GluLeu: 5.114 ± 0.089	1.605GluMet: 1.605 ± 0.047	1.821GluAsn: 1.821 ± 0.047	1.917GluPro: 1.917 ± 0.056	2.653GluGln: 2.653 ± 0.062	4.447GluArg: 4.447 ± 0.095	3.018GluSer: 3.018 ± 0.066	2.94GluThr: 2.94 ± 0.065	3.738GluVal: 3.738 ± 0.075	0.807GluTrp: 0.807 ± 0.038	1.293GluTyr: 1.293 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.796PheAla: 3.796 ± 0.076	0.476PheCys: 0.476 ± 0.026	1.97PheAsp: 1.97 ± 0.051	1.498PheGlu: 1.498 ± 0.046	1.67PhePhe: 1.67 ± 0.052	2.925PheGly: 2.925 ± 0.065	0.965PheHis: 0.965 ± 0.036	1.893PheIle: 1.893 ± 0.049	0.953PheLys: 0.953 ± 0.042	3.914PheLeu: 3.914 ± 0.081	0.883PheMet: 0.883 ± 0.037	1.164PheAsn: 1.164 ± 0.039	1.739PhePro: 1.739 ± 0.05	1.22PheGln: 1.22 ± 0.042	2.015PheArg: 2.015 ± 0.052	2.423PheSer: 2.423 ± 0.057	1.863PheThr: 1.863 ± 0.043	2.406PheVal: 2.406 ± 0.063	0.68PheTrp: 0.68 ± 0.029	1.157PheTyr: 1.157 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	7.527GlyAla: 7.527 ± 0.11	0.86GlyCys: 0.86 ± 0.036	3.91GlyAsp: 3.91 ± 0.064	4.231GlyGlu: 4.231 ± 0.074	3.136GlyPhe: 3.136 ± 0.071	6.374GlyGly: 6.374 ± 0.113	2.234GlyHis: 2.234 ± 0.06	4.815GlyIle: 4.815 ± 0.079	3.51GlyLys: 3.51 ± 0.073	8.151GlyLeu: 8.151 ± 0.127	2.469GlyMet: 2.469 ± 0.062	2.563GlyAsn: 2.563 ± 0.072	2.945GlyPro: 2.945 ± 0.063	3.375GlyGln: 3.375 ± 0.075	4.979GlyArg: 4.979 ± 0.087	4.124GlySer: 4.124 ± 0.077	4.093GlyThr: 4.093 ± 0.08	6.155GlyVal: 6.155 ± 0.105	1.38GlyTrp: 1.38 ± 0.043	2.322GlyTyr: 2.322 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	2.652HisAla: 2.652 ± 0.059	0.377HisCys: 0.377 ± 0.02	1.297HisAsp: 1.297 ± 0.041	1.209HisGlu: 1.209 ± 0.04	1.131HisPhe: 1.131 ± 0.04	2.299HisGly: 2.299 ± 0.059	0.838HisHis: 0.838 ± 0.033	1.423HisIle: 1.423 ± 0.046	0.759HisLys: 0.759 ± 0.029	3.105HisLeu: 3.105 ± 0.072	0.635HisMet: 0.635 ± 0.028	0.742HisAsn: 0.742 ± 0.027	1.848HisPro: 1.848 ± 0.054	1.054HisGln: 1.054 ± 0.031	1.755HisArg: 1.755 ± 0.049	1.344HisSer: 1.344 ± 0.041	1.237HisThr: 1.237 ± 0.038	1.611HisVal: 1.611 ± 0.042	0.598HisTrp: 0.598 ± 0.025	0.917HisTyr: 0.917 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.258IleAla: 6.258 ± 0.092	0.548IleCys: 0.548 ± 0.029	2.773IleAsp: 2.773 ± 0.065	2.926IleGlu: 2.926 ± 0.068	1.954IlePhe: 1.954 ± 0.063	4.385IleGly: 4.385 ± 0.083	1.61IleHis: 1.61 ± 0.042	2.942IleIle: 2.942 ± 0.07	1.695IleLys: 1.695 ± 0.052	5.962IleLeu: 5.962 ± 0.089	1.114IleMet: 1.114 ± 0.039	1.773IleAsn: 1.773 ± 0.055	3.108IlePro: 3.108 ± 0.065	2.046IleGln: 2.046 ± 0.053	3.375IleArg: 3.375 ± 0.07	3.398IleSer: 3.398 ± 0.058	3.027IleThr: 3.027 ± 0.07	3.565IleVal: 3.565 ± 0.072	0.67IleTrp: 0.67 ± 0.028	1.429IleTyr: 1.429 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	3.914LysAla: 3.914 ± 0.078	0.189LysCys: 0.189 ± 0.019	1.807LysAsp: 1.807 ± 0.05	1.912LysGlu: 1.912 ± 0.064	0.82LysPhe: 0.82 ± 0.032	2.458LysGly: 2.458 ± 0.05	0.749LysHis: 0.749 ± 0.033	1.994LysIle: 1.994 ± 0.065	1.664LysLys: 1.664 ± 0.058	2.956LysLeu: 2.956 ± 0.066	0.944LysMet: 0.944 ± 0.032	1.289LysAsn: 1.289 ± 0.045	1.747LysPro: 1.747 ± 0.052	1.209LysGln: 1.209 ± 0.045	2.293LysArg: 2.293 ± 0.064	2.025LysSer: 2.025 ± 0.058	2.13LysThr: 2.13 ± 0.056	2.304LysVal: 2.304 ± 0.06	0.36LysTrp: 0.36 ± 0.021	0.749LysTyr: 0.749 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.73LeuAla: 12.73 ± 0.142	1.0LeuCys: 1.0 ± 0.038	5.633LeuAsp: 5.633 ± 0.092	5.944LeuGlu: 5.944 ± 0.103	3.803LeuPhe: 3.803 ± 0.065	8.118LeuGly: 8.118 ± 0.118	2.853LeuHis: 2.853 ± 0.064	4.955LeuIle: 4.955 ± 0.082	3.355LeuLys: 3.355 ± 0.072	12.105LeuLeu: 12.105 ± 0.168	2.472LeuMet: 2.472 ± 0.058	3.006LeuAsn: 3.006 ± 0.071	6.319LeuPro: 6.319 ± 0.087	5.089LeuGln: 5.089 ± 0.083	8.735LeuArg: 8.735 ± 0.143	6.517LeuSer: 6.517 ± 0.092	5.171LeuThr: 5.171 ± 0.072	6.92LeuVal: 6.92 ± 0.104	1.643LeuTrp: 1.643 ± 0.053	2.547LeuTyr: 2.547 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	3.214MetAla: 3.214 ± 0.058	0.147MetCys: 0.147 ± 0.012	1.532MetAsp: 1.532 ± 0.042	1.54MetGlu: 1.54 ± 0.048	0.579MetPhe: 0.579 ± 0.027	2.132MetGly: 2.132 ± 0.06	0.643MetHis: 0.643 ± 0.03	1.284MetIle: 1.284 ± 0.044	0.932MetLys: 0.932 ± 0.038	2.521MetLeu: 2.521 ± 0.058	0.607MetMet: 0.607 ± 0.03	0.939MetAsn: 0.939 ± 0.033	1.469MetPro: 1.469 ± 0.043	1.077MetGln: 1.077 ± 0.032	1.792MetArg: 1.792 ± 0.052	1.556MetSer: 1.556 ± 0.041	1.54MetThr: 1.54 ± 0.04	1.935MetVal: 1.935 ± 0.046	0.205MetTrp: 0.205 ± 0.016	0.371MetTyr: 0.371 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.147AsnAla: 3.147 ± 0.069	0.26AsnCys: 0.26 ± 0.021	1.444AsnAsp: 1.444 ± 0.044	1.25AsnGlu: 1.25 ± 0.039	1.03AsnPhe: 1.03 ± 0.037	2.544AsnGly: 2.544 ± 0.067	0.734AsnHis: 0.734 ± 0.031	1.818AsnIle: 1.818 ± 0.05	0.979AsnLys: 0.979 ± 0.037	3.027AsnLeu: 3.027 ± 0.067	0.689AsnMet: 0.689 ± 0.028	1.038AsnAsn: 1.038 ± 0.046	2.168AsnPro: 2.168 ± 0.063	1.118AsnGln: 1.118 ± 0.045	1.878AsnArg: 1.878 ± 0.048	1.525AsnSer: 1.525 ± 0.053	1.706AsnThr: 1.706 ± 0.054	1.881AsnVal: 1.881 ± 0.052	0.466AsnTrp: 0.466 ± 0.025	0.828AsnTyr: 0.828 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	5.434ProAla: 5.434 ± 0.087	0.419ProCys: 0.419 ± 0.022	3.197ProAsp: 3.197 ± 0.065	3.712ProGlu: 3.712 ± 0.079	1.831ProPhe: 1.831 ± 0.049	4.18ProGly: 4.18 ± 0.073	1.242ProHis: 1.242 ± 0.042	2.48ProIle: 2.48 ± 0.061	1.597ProLys: 1.597 ± 0.053	5.372ProLeu: 5.372 ± 0.092	1.354ProMet: 1.354 ± 0.041	1.303ProAsn: 1.303 ± 0.041	2.273ProPro: 2.273 ± 0.061	2.224ProGln: 2.224 ± 0.053	2.706ProArg: 2.706 ± 0.067	2.653ProSer: 2.653 ± 0.065	2.349ProThr: 2.349 ± 0.055	4.086ProVal: 4.086 ± 0.067	0.885ProTrp: 0.885 ± 0.041	1.445ProTyr: 1.445 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	4.884GlnAla: 4.884 ± 0.096	0.35GlnCys: 0.35 ± 0.021	1.865GlnAsp: 1.865 ± 0.049	2.553GlnGlu: 2.553 ± 0.066	1.234GlnPhe: 1.234 ± 0.042	3.285GlnGly: 3.285 ± 0.072	1.231GlnHis: 1.231 ± 0.044	2.414GlnIle: 2.414 ± 0.062	1.648GlnLys: 1.648 ± 0.051	3.723GlnLeu: 3.723 ± 0.073	1.207GlnMet: 1.207 ± 0.037	1.244GlnAsn: 1.244 ± 0.038	2.027GlnPro: 2.027 ± 0.051	2.303GlnGln: 2.303 ± 0.059	3.51GlnArg: 3.51 ± 0.077	2.509GlnSer: 2.509 ± 0.062	2.131GlnThr: 2.131 ± 0.048	3.097GlnVal: 3.097 ± 0.064	0.734GlnTrp: 0.734 ± 0.034	1.038GlnTyr: 1.038 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	6.418ArgAla: 6.418 ± 0.105	0.669ArgCys: 0.669 ± 0.029	3.518ArgAsp: 3.518 ± 0.07	4.112ArgGlu: 4.112 ± 0.084	2.759ArgPhe: 2.759 ± 0.063	4.311ArgGly: 4.311 ± 0.092	2.144ArgHis: 2.144 ± 0.059	3.864ArgIle: 3.864 ± 0.062	2.458ArgLys: 2.458 ± 0.066	8.086ArgLeu: 8.086 ± 0.13	1.844ArgMet: 1.844 ± 0.053	1.977ArgAsn: 1.977 ± 0.05	3.108ArgPro: 3.108 ± 0.059	3.38ArgGln: 3.38 ± 0.066	5.276ArgArg: 5.276 ± 0.104	3.454ArgSer: 3.454 ± 0.075	2.662ArgThr: 2.662 ± 0.058	4.502ArgVal: 4.502 ± 0.08	1.235ArgTrp: 1.235 ± 0.042	2.214ArgTyr: 2.214 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	6.267SerAla: 6.267 ± 0.103	0.548SerCys: 0.548 ± 0.026	2.664SerAsp: 2.664 ± 0.06	2.551SerGlu: 2.551 ± 0.063	2.05SerPhe: 2.05 ± 0.057	5.359SerGly: 5.359 ± 0.091	1.355SerHis: 1.355 ± 0.039	2.853SerIle: 2.853 ± 0.061	1.635SerLys: 1.635 ± 0.052	5.871SerLeu: 5.871 ± 0.091	1.585SerMet: 1.585 ± 0.046	1.581SerAsn: 1.581 ± 0.058	2.973SerPro: 2.973 ± 0.061	2.047SerGln: 2.047 ± 0.051	3.23SerArg: 3.23 ± 0.068	3.15SerSer: 3.15 ± 0.079	2.978SerThr: 2.978 ± 0.063	3.721SerVal: 3.721 ± 0.064	0.862SerTrp: 0.862 ± 0.038	1.387SerTyr: 1.387 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	5.615ThrAla: 5.615 ± 0.088	0.475ThrCys: 0.475 ± 0.024	2.508ThrAsp: 2.508 ± 0.06	2.524ThrGlu: 2.524 ± 0.051	1.703ThrPhe: 1.703 ± 0.047	4.612ThrGly: 4.612 ± 0.08	1.328ThrHis: 1.328 ± 0.042	2.734ThrIle: 2.734 ± 0.064	1.312ThrLys: 1.312 ± 0.043	6.198ThrLeu: 6.198 ± 0.091	1.084ThrMet: 1.084 ± 0.036	1.345ThrAsn: 1.345 ± 0.046	3.241ThrPro: 3.241 ± 0.06	1.948ThrGln: 1.948 ± 0.053	3.02ThrArg: 3.02 ± 0.066	2.503ThrSer: 2.503 ± 0.058	2.658ThrThr: 2.658 ± 0.06	3.814ThrVal: 3.814 ± 0.079	0.729ThrTrp: 0.729 ± 0.031	1.26ThrTyr: 1.26 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	7.915ValAla: 7.915 ± 0.11	0.638ValCys: 0.638 ± 0.028	3.789ValAsp: 3.789 ± 0.071	3.87ValGlu: 3.87 ± 0.073	2.549ValPhe: 2.549 ± 0.06	5.194ValGly: 5.194 ± 0.087	1.762ValHis: 1.762 ± 0.054	3.987ValIle: 3.987 ± 0.076	2.027ValLys: 2.027 ± 0.062	7.907ValLeu: 7.907 ± 0.112	1.744ValMet: 1.744 ± 0.049	2.05ValAsn: 2.05 ± 0.063	3.464ValPro: 3.464 ± 0.073	2.727ValGln: 2.727 ± 0.06	4.513ValArg: 4.513 ± 0.078	4.12ValSer: 4.12 ± 0.072	3.847ValThr: 3.847 ± 0.066	5.259ValVal: 5.259 ± 0.089	1.056ValTrp: 1.056 ± 0.041	1.507ValTyr: 1.507 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.326TrpAla: 1.326 ± 0.044	0.158TrpCys: 0.158 ± 0.015	0.676TrpAsp: 0.676 ± 0.032	0.801TrpGlu: 0.801 ± 0.031	0.537TrpPhe: 0.537 ± 0.027	1.143TrpGly: 1.143 ± 0.045	0.428TrpHis: 0.428 ± 0.023	0.846TrpIle: 0.846 ± 0.044	0.511TrpLys: 0.511 ± 0.025	2.078TrpLeu: 2.078 ± 0.058	0.462TrpMet: 0.462 ± 0.024	0.478TrpAsn: 0.478 ± 0.027	0.804TrpPro: 0.804 ± 0.03	0.899TrpGln: 0.899 ± 0.037	1.33TrpArg: 1.33 ± 0.044	0.831TrpSer: 0.831 ± 0.033	0.74TrpThr: 0.74 ± 0.032	1.184TrpVal: 1.184 ± 0.04	0.329TrpTrp: 0.329 ± 0.02	0.386TrpTyr: 0.386 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.658TyrAla: 2.658 ± 0.067	0.34TyrCys: 0.34 ± 0.02	1.27TyrAsp: 1.27 ± 0.043	1.158TyrGlu: 1.158 ± 0.041	1.085TyrPhe: 1.085 ± 0.038	2.297TyrGly: 2.297 ± 0.057	0.806TyrHis: 0.806 ± 0.032	1.116TyrIle: 1.116 ± 0.04	0.696TyrLys: 0.696 ± 0.035	3.029TyrLeu: 3.029 ± 0.06	0.532TyrMet: 0.532 ± 0.027	0.792TyrAsn: 0.792 ± 0.035	1.425TyrPro: 1.425 ± 0.043	1.276TyrGln: 1.276 ± 0.039	2.015TyrArg: 2.015 ± 0.048	1.407TyrSer: 1.407 ± 0.044	1.292TyrThr: 1.292 ± 0.04	1.765TyrVal: 1.765 ± 0.045	0.456TyrTrp: 0.456 ± 0.024	0.792TyrTyr: 0.792 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2816 proteins (808942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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