Nostocales cyanobacterium HT-58-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; unclassified Nostocales

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0RNJ5|A0A1Y0RNJ5_9CYAN Gamma-glutamyl phosphate reductase OS=Nostocales cyanobacterium HT-58-2 OX=1940762 GN=proA PE=3 SV=1
MM1 pKa = 7.59SNNSIQGNGNWYY13 pKa = 7.52YY14 pKa = 11.25TNGQWEE20 pKa = 4.41SDD22 pKa = 3.4EE23 pKa = 4.74GADD26 pKa = 5.3LSVADD31 pKa = 5.21AFASGNPPAGYY42 pKa = 9.9IPDD45 pKa = 4.7NISSATGNPLAGSSSSSSSGTDD67 pKa = 3.52FGGSIPFAGSGNSSSDD83 pKa = 3.26NPFAGGNPFMIGGGGNVSTTSADD106 pKa = 3.18GQYY109 pKa = 9.97TYY111 pKa = 11.26NYY113 pKa = 8.37TRR115 pKa = 11.84TADD118 pKa = 3.54ARR120 pKa = 11.84SLVPDD125 pKa = 4.75DD126 pKa = 4.45NSPFAKK132 pKa = 10.46LLGVLGVGSDD142 pKa = 3.49SSSNSGSNPFAGGSNPFSGGSTPTGDD168 pKa = 3.81TNPQLGGNNPYY179 pKa = 10.29FAGNGSGNSALGSSDD194 pKa = 3.68KK195 pKa = 11.04FPGNLPIANTAPSSTSDD212 pKa = 3.61SILPEE217 pKa = 4.18APEE220 pKa = 4.4GLSVPYY226 pKa = 10.53NSNNWISDD234 pKa = 3.63LKK236 pKa = 10.74DD237 pKa = 3.56LQSGEE242 pKa = 4.53PGASTSGNTGSGNGNWFYY260 pKa = 11.64ASNNTANGNGNWYY273 pKa = 10.33FSDD276 pKa = 4.29GNTTDD281 pKa = 5.06GNGNWFYY288 pKa = 11.66ASNNTANGNGNWYY301 pKa = 9.92FGSGNTTDD309 pKa = 5.59GNGNWSSGGNNTINGNGNRR328 pKa = 11.84PSGSGNTINGNGNKK342 pKa = 9.76PSGSGNNILGNANTSNTSGNNILGNHH368 pKa = 5.75VEE370 pKa = 4.11TSGDD374 pKa = 2.99GKK376 pKa = 11.03AYY378 pKa = 9.82IGNDD382 pKa = 2.9DD383 pKa = 3.52WSFDD387 pKa = 3.4ILNEE391 pKa = 4.07LKK393 pKa = 10.58SLQSGVSGVGSDD405 pKa = 3.79VNNFLSNPDD414 pKa = 4.36LISTTTSKK422 pKa = 10.98EE423 pKa = 3.98GFSNSPFLTNSNYY436 pKa = 9.77QFDD439 pKa = 4.37FSSGLL444 pKa = 3.5

Molecular weight: 44.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0RN93|A0A1Y0RN93_9CYAN Uncharacterized protein OS=Nostocales cyanobacterium HT-58-2 OX=1940762 GN=BZZ01_16335 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.43LNYY5 pKa = 9.15RR6 pKa = 11.84RR7 pKa = 11.84CISCRR12 pKa = 11.84KK13 pKa = 9.63VGLKK17 pKa = 9.99QEE19 pKa = 4.31FWRR22 pKa = 11.84IVRR25 pKa = 11.84VFPSGQVQLDD35 pKa = 3.42EE36 pKa = 4.54GMGRR40 pKa = 11.84SAYY43 pKa = 9.38ICPQQSCLAAAQKK56 pKa = 10.6KK57 pKa = 9.1NRR59 pKa = 11.84LGRR62 pKa = 11.84SLRR65 pKa = 11.84ASVPEE70 pKa = 3.86ALYY73 pKa = 10.57HH74 pKa = 6.78ILQQRR79 pKa = 11.84LSQSHH84 pKa = 5.26NQKK87 pKa = 8.18QTT89 pKa = 2.89

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6085

0

6085

2015771

24

4291

331.3

36.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.129 ± 0.034

1.023 ± 0.011

4.677 ± 0.024

6.275 ± 0.035

3.978 ± 0.022

6.68 ± 0.037

1.961 ± 0.015

6.439 ± 0.025

4.849 ± 0.03

10.976 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.013

4.179 ± 0.022

4.744 ± 0.025

5.489 ± 0.033

5.213 ± 0.025

6.462 ± 0.027

5.724 ± 0.023

6.938 ± 0.026

1.422 ± 0.012

3.005 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski