Amino acid dipepetide frequency for Lachnotalea sp. AF33-28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.146AlaAla: 9.146 ± 0.106
1.339AlaCys: 1.339 ± 0.033
5.104AlaAsp: 5.104 ± 0.065
5.4AlaGlu: 5.4 ± 0.069
3.227AlaPhe: 3.227 ± 0.055
7.101AlaGly: 7.101 ± 0.081
1.161AlaHis: 1.161 ± 0.031
4.163AlaIle: 4.163 ± 0.065
4.023AlaLys: 4.023 ± 0.054
7.71AlaLeu: 7.71 ± 0.077
2.411AlaMet: 2.411 ± 0.043
2.245AlaAsn: 2.245 ± 0.041
2.306AlaPro: 2.306 ± 0.042
2.341AlaGln: 2.341 ± 0.041
3.528AlaArg: 3.528 ± 0.052
4.067AlaSer: 4.067 ± 0.058
2.563AlaThr: 2.563 ± 0.055
7.25AlaVal: 7.25 ± 0.083
0.818AlaTrp: 0.818 ± 0.026
2.98AlaTyr: 2.98 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.24CysAla: 1.24 ± 0.029
0.358CysCys: 0.358 ± 0.014
0.887CysAsp: 0.887 ± 0.025
1.004CysGlu: 1.004 ± 0.027
0.77CysPhe: 0.77 ± 0.026
1.731CysGly: 1.731 ± 0.038
0.349CysHis: 0.349 ± 0.015
1.161CysIle: 1.161 ± 0.032
0.61CysLys: 0.61 ± 0.02
1.462CysLeu: 1.462 ± 0.033
0.55CysMet: 0.55 ± 0.022
0.491CysAsn: 0.491 ± 0.017
0.71CysPro: 0.71 ± 0.025
0.436CysGln: 0.436 ± 0.018
1.199CysArg: 1.199 ± 0.029
1.062CysSer: 1.062 ± 0.026
0.787CysThr: 0.787 ± 0.024
1.065CysVal: 1.065 ± 0.029
0.17CysTrp: 0.17 ± 0.012
0.669CysTyr: 0.669 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.235AspAla: 4.235 ± 0.064
0.96AspCys: 0.96 ± 0.028
2.471AspAsp: 2.471 ± 0.054
4.32AspGlu: 4.32 ± 0.063
2.588AspPhe: 2.588 ± 0.04
4.885AspGly: 4.885 ± 0.07
0.906AspHis: 0.906 ± 0.024
4.003AspIle: 4.003 ± 0.057
2.9AspLys: 2.9 ± 0.048
4.856AspLeu: 4.856 ± 0.065
1.845AspMet: 1.845 ± 0.037
1.95AspAsn: 1.95 ± 0.038
2.098AspPro: 2.098 ± 0.041
1.472AspGln: 1.472 ± 0.033
3.269AspArg: 3.269 ± 0.051
3.204AspSer: 3.204 ± 0.057
3.055AspThr: 3.055 ± 0.049
3.391AspVal: 3.391 ± 0.048
0.76AspTrp: 0.76 ± 0.022
2.838AspTyr: 2.838 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
5.782GluAla: 5.782 ± 0.072
0.917GluCys: 0.917 ± 0.026
4.275GluAsp: 4.275 ± 0.055
6.815GluGlu: 6.815 ± 0.094
2.565GluPhe: 2.565 ± 0.044
4.536GluGly: 4.536 ± 0.068
1.405GluHis: 1.405 ± 0.034
4.916GluIle: 4.916 ± 0.057
5.23GluLys: 5.23 ± 0.07
6.802GluLeu: 6.802 ± 0.077
2.103GluMet: 2.103 ± 0.038
3.61GluAsn: 3.61 ± 0.045
2.093GluPro: 2.093 ± 0.039
2.862GluGln: 2.862 ± 0.054
3.72GluArg: 3.72 ± 0.054
3.533GluSer: 3.533 ± 0.057
3.729GluThr: 3.729 ± 0.059
3.93GluVal: 3.93 ± 0.053
0.799GluTrp: 0.799 ± 0.027
3.226GluTyr: 3.226 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
3.121PheAla: 3.121 ± 0.051
0.842PheCys: 0.842 ± 0.021
2.482PheAsp: 2.482 ± 0.039
2.584PheGlu: 2.584 ± 0.052
1.911PhePhe: 1.911 ± 0.045
3.046PheGly: 3.046 ± 0.053
0.889PheHis: 0.889 ± 0.026
2.855PheIle: 2.855 ± 0.05
1.82PheLys: 1.82 ± 0.035
4.293PheLeu: 4.293 ± 0.081
1.175PheMet: 1.175 ± 0.032
1.507PheAsn: 1.507 ± 0.029
1.505PhePro: 1.505 ± 0.034
1.328PheGln: 1.328 ± 0.029
1.949PheArg: 1.949 ± 0.039
2.977PheSer: 2.977 ± 0.051
2.569PheThr: 2.569 ± 0.049
2.601PheVal: 2.601 ± 0.044
0.537PheTrp: 0.537 ± 0.02
1.98PheTyr: 1.98 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
5.13GlyAla: 5.13 ± 0.068
1.548GlyCys: 1.548 ± 0.032
3.573GlyAsp: 3.573 ± 0.059
5.018GlyGlu: 5.018 ± 0.07
3.19GlyPhe: 3.19 ± 0.05
5.318GlyGly: 5.318 ± 0.091
1.321GlyHis: 1.321 ± 0.033
6.035GlyIle: 6.035 ± 0.063
4.343GlyLys: 4.343 ± 0.057
6.594GlyLeu: 6.594 ± 0.066
2.627GlyMet: 2.627 ± 0.047
2.828GlyAsn: 2.828 ± 0.05
1.536GlyPro: 1.536 ± 0.035
2.279GlyGln: 2.279 ± 0.046
4.33GlyArg: 4.33 ± 0.057
4.437GlySer: 4.437 ± 0.057
4.075GlyThr: 4.075 ± 0.06
4.687GlyVal: 4.687 ± 0.063
0.934GlyTrp: 0.934 ± 0.026
3.495GlyTyr: 3.495 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.183HisAla: 1.183 ± 0.027
0.353HisCys: 0.353 ± 0.015
0.857HisAsp: 0.857 ± 0.025
1.046HisGlu: 1.046 ± 0.024
0.903HisPhe: 0.903 ± 0.025
1.328HisGly: 1.328 ± 0.029
0.377HisHis: 0.377 ± 0.018
1.424HisIle: 1.424 ± 0.028
0.78HisLys: 0.78 ± 0.025
1.596HisLeu: 1.596 ± 0.031
0.662HisMet: 0.662 ± 0.021
0.717HisAsn: 0.717 ± 0.023
1.0HisPro: 1.0 ± 0.027
0.52HisGln: 0.52 ± 0.018
0.932HisArg: 0.932 ± 0.027
1.036HisSer: 1.036 ± 0.026
0.986HisThr: 0.986 ± 0.031
1.099HisVal: 1.099 ± 0.026
0.251HisTrp: 0.251 ± 0.015
0.845HisTyr: 0.845 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.275IleAla: 5.275 ± 0.07
1.322IleCys: 1.322 ± 0.031
3.44IleAsp: 3.44 ± 0.053
3.973IleGlu: 3.973 ± 0.058
2.719IlePhe: 2.719 ± 0.05
4.751IleGly: 4.751 ± 0.066
1.29IleHis: 1.29 ± 0.028
4.055IleIle: 4.055 ± 0.073
3.132IleLys: 3.132 ± 0.054
6.771IleLeu: 6.771 ± 0.086
1.785IleMet: 1.785 ± 0.035
2.491IleAsn: 2.491 ± 0.041
3.106IlePro: 3.106 ± 0.048
2.106IleGln: 2.106 ± 0.039
4.277IleArg: 4.277 ± 0.054
4.644IleSer: 4.644 ± 0.064
3.927IleThr: 3.927 ± 0.051
4.231IleVal: 4.231 ± 0.066
0.763IleTrp: 0.763 ± 0.023
2.778IleTyr: 2.778 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
4.509LysAla: 4.509 ± 0.059
0.619LysCys: 0.619 ± 0.024
3.397LysAsp: 3.397 ± 0.052
5.372LysGlu: 5.372 ± 0.069
1.525LysPhe: 1.525 ± 0.035
3.596LysGly: 3.596 ± 0.052
0.934LysHis: 0.934 ± 0.025
3.572LysIle: 3.572 ± 0.047
4.478LysLys: 4.478 ± 0.062
4.711LysLeu: 4.711 ± 0.056
1.717LysMet: 1.717 ± 0.03
2.823LysAsn: 2.823 ± 0.042
1.873LysPro: 1.873 ± 0.038
2.087LysGln: 2.087 ± 0.042
3.107LysArg: 3.107 ± 0.043
3.011LysSer: 3.011 ± 0.048
3.156LysThr: 3.156 ± 0.048
3.163LysVal: 3.163 ± 0.055
0.573LysTrp: 0.573 ± 0.019
2.422LysTyr: 2.422 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
7.347LeuAla: 7.347 ± 0.081
1.847LeuCys: 1.847 ± 0.037
5.319LeuAsp: 5.319 ± 0.063
6.494LeuGlu: 6.494 ± 0.079
4.384LeuPhe: 4.384 ± 0.07
5.811LeuGly: 5.811 ± 0.07
1.683LeuHis: 1.683 ± 0.035
6.153LeuIle: 6.153 ± 0.085
6.278LeuLys: 6.278 ± 0.058
9.884LeuLeu: 9.884 ± 0.132
3.007LeuMet: 3.007 ± 0.047
3.9LeuAsn: 3.9 ± 0.052
4.153LeuPro: 4.153 ± 0.051
2.957LeuGln: 2.957 ± 0.041
4.146LeuArg: 4.146 ± 0.057
6.512LeuSer: 6.512 ± 0.074
5.538LeuThr: 5.538 ± 0.058
5.249LeuVal: 5.249 ± 0.066
1.07LeuTrp: 1.07 ± 0.03
4.061LeuTyr: 4.061 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.466MetAla: 2.466 ± 0.042
0.395MetCys: 0.395 ± 0.017
1.9MetAsp: 1.9 ± 0.035
2.704MetGlu: 2.704 ± 0.04
1.002MetPhe: 1.002 ± 0.024
2.144MetGly: 2.144 ± 0.036
0.48MetHis: 0.48 ± 0.02
2.05MetIle: 2.05 ± 0.037
2.359MetLys: 2.359 ± 0.037
3.083MetLeu: 3.083 ± 0.047
0.892MetMet: 0.892 ± 0.023
1.457MetAsn: 1.457 ± 0.03
1.263MetPro: 1.263 ± 0.027
1.082MetGln: 1.082 ± 0.026
1.499MetArg: 1.499 ± 0.032
1.732MetSer: 1.732 ± 0.034
1.699MetThr: 1.699 ± 0.031
1.816MetVal: 1.816 ± 0.037
0.281MetTrp: 0.281 ± 0.015
0.98MetTyr: 0.98 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.984AsnAla: 2.984 ± 0.05
0.577AsnCys: 0.577 ± 0.02
1.845AsnAsp: 1.845 ± 0.039
2.45AsnGlu: 2.45 ± 0.041
1.486AsnPhe: 1.486 ± 0.032
3.301AsnGly: 3.301 ± 0.052
0.742AsnHis: 0.742 ± 0.021
2.834AsnIle: 2.834 ± 0.049
1.859AsnLys: 1.859 ± 0.038
3.595AsnLeu: 3.595 ± 0.049
1.265AsnMet: 1.265 ± 0.031
1.434AsnAsn: 1.434 ± 0.047
1.947AsnPro: 1.947 ± 0.036
1.259AsnGln: 1.259 ± 0.032
2.384AsnArg: 2.384 ± 0.035
2.334AsnSer: 2.334 ± 0.046
2.157AsnThr: 2.157 ± 0.038
2.385AsnVal: 2.385 ± 0.043
0.472AsnTrp: 0.472 ± 0.018
1.803AsnTyr: 1.803 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
3.164ProAla: 3.164 ± 0.056
0.527ProCys: 0.527 ± 0.018
2.763ProAsp: 2.763 ± 0.049
3.47ProGlu: 3.47 ± 0.056
1.729ProPhe: 1.729 ± 0.037
2.761ProGly: 2.761 ± 0.044
0.7ProHis: 0.7 ± 0.027
1.955ProIle: 1.955 ± 0.034
1.733ProLys: 1.733 ± 0.036
3.239ProLeu: 3.239 ± 0.053
0.982ProMet: 0.982 ± 0.028
1.219ProAsn: 1.219 ± 0.026
0.92ProPro: 0.92 ± 0.027
1.13ProGln: 1.13 ± 0.029
1.23ProArg: 1.23 ± 0.029
2.055ProSer: 2.055 ± 0.037
1.453ProThr: 1.453 ± 0.035
3.33ProVal: 3.33 ± 0.055
0.426ProTrp: 0.426 ± 0.019
1.613ProTyr: 1.613 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.543GlnAla: 2.543 ± 0.045
0.362GlnCys: 0.362 ± 0.016
1.692GlnAsp: 1.692 ± 0.03
2.604GlnGlu: 2.604 ± 0.047
1.19GlnPhe: 1.19 ± 0.026
1.904GlnGly: 1.904 ± 0.035
0.466GlnHis: 0.466 ± 0.018
2.48GlnIle: 2.48 ± 0.041
2.354GlnLys: 2.354 ± 0.04
3.036GlnLeu: 3.036 ± 0.048
1.205GlnMet: 1.205 ± 0.032
1.534GlnAsn: 1.534 ± 0.035
1.051GlnPro: 1.051 ± 0.029
1.19GlnGln: 1.19 ± 0.031
1.509GlnArg: 1.509 ± 0.036
1.795GlnSer: 1.795 ± 0.037
1.757GlnThr: 1.757 ± 0.038
1.955GlnVal: 1.955 ± 0.035
0.386GlnTrp: 0.386 ± 0.016
1.45GlnTyr: 1.45 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
3.283ArgAla: 3.283 ± 0.043
0.816ArgCys: 0.816 ± 0.025
2.605ArgAsp: 2.605 ± 0.042
4.295ArgGlu: 4.295 ± 0.065
2.334ArgPhe: 2.334 ± 0.041
2.984ArgGly: 2.984 ± 0.043
0.978ArgHis: 0.978 ± 0.029
4.194ArgIle: 4.194 ± 0.045
3.131ArgLys: 3.131 ± 0.045
5.201ArgLeu: 5.201 ± 0.062
1.971ArgMet: 1.971 ± 0.042
2.023ArgAsn: 2.023 ± 0.033
1.822ArgPro: 1.822 ± 0.043
2.181ArgGln: 2.181 ± 0.041
3.142ArgArg: 3.142 ± 0.052
2.625ArgSer: 2.625 ± 0.038
2.623ArgThr: 2.623 ± 0.044
2.952ArgVal: 2.952 ± 0.043
0.584ArgTrp: 0.584 ± 0.019
2.437ArgTyr: 2.437 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
4.76SerAla: 4.76 ± 0.06
1.024SerCys: 1.024 ± 0.028
3.183SerAsp: 3.183 ± 0.047
3.626SerGlu: 3.626 ± 0.054
2.712SerPhe: 2.712 ± 0.052
5.395SerGly: 5.395 ± 0.071
1.091SerHis: 1.091 ± 0.028
3.767SerIle: 3.767 ± 0.056
2.596SerLys: 2.596 ± 0.052
5.557SerLeu: 5.557 ± 0.062
1.92SerMet: 1.92 ± 0.035
2.006SerAsn: 2.006 ± 0.043
2.025SerPro: 2.025 ± 0.04
1.833SerGln: 1.833 ± 0.034
3.231SerArg: 3.231 ± 0.046
3.512SerSer: 3.512 ± 0.069
2.614SerThr: 2.614 ± 0.038
4.466SerVal: 4.466 ± 0.054
0.738SerTrp: 0.738 ± 0.022
2.687SerTyr: 2.687 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.066ThrAla: 5.066 ± 0.07
0.682ThrCys: 0.682 ± 0.021
3.449ThrAsp: 3.449 ± 0.058
3.687ThrGlu: 3.687 ± 0.061
2.06ThrPhe: 2.06 ± 0.034
4.706ThrGly: 4.706 ± 0.054
0.85ThrHis: 0.85 ± 0.026
2.874ThrIle: 2.874 ± 0.049
2.325ThrLys: 2.325 ± 0.042
4.87ThrLeu: 4.87 ± 0.06
1.388ThrMet: 1.388 ± 0.03
1.697ThrAsn: 1.697 ± 0.039
2.132ThrPro: 2.132 ± 0.039
1.507ThrGln: 1.507 ± 0.031
1.979ThrArg: 1.979 ± 0.038
2.588ThrSer: 2.588 ± 0.046
2.323ThrThr: 2.323 ± 0.046
5.089ThrVal: 5.089 ± 0.058
0.512ThrTrp: 0.512 ± 0.021
1.998ThrTyr: 1.998 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
3.985ValAla: 3.985 ± 0.054
1.289ValCys: 1.289 ± 0.03
3.536ValAsp: 3.536 ± 0.05
4.034ValGlu: 4.034 ± 0.054
3.082ValPhe: 3.082 ± 0.044
3.895ValGly: 3.895 ± 0.06
1.105ValHis: 1.105 ± 0.026
4.781ValIle: 4.781 ± 0.059
4.034ValLys: 4.034 ± 0.048
7.009ValLeu: 7.009 ± 0.075
2.12ValMet: 2.12 ± 0.04
2.784ValAsn: 2.784 ± 0.045
2.713ValPro: 2.713 ± 0.043
1.889ValGln: 1.889 ± 0.038
3.492ValArg: 3.492 ± 0.046
4.515ValSer: 4.515 ± 0.056
3.946ValThr: 3.946 ± 0.063
4.02ValVal: 4.02 ± 0.062
0.804ValTrp: 0.804 ± 0.024
2.836ValTyr: 2.836 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.687TrpAla: 0.687 ± 0.024
0.193TrpCys: 0.193 ± 0.011
0.662TrpAsp: 0.662 ± 0.021
0.848TrpGlu: 0.848 ± 0.026
0.504TrpPhe: 0.504 ± 0.021
0.781TrpGly: 0.781 ± 0.024
0.243TrpHis: 0.243 ± 0.014
0.717TrpIle: 0.717 ± 0.021
0.819TrpLys: 0.819 ± 0.024
1.133TrpLeu: 1.133 ± 0.028
0.443TrpMet: 0.443 ± 0.019
0.652TrpAsn: 0.652 ± 0.023
0.329TrpPro: 0.329 ± 0.016
0.495TrpGln: 0.495 ± 0.021
0.52TrpArg: 0.52 ± 0.018
0.624TrpSer: 0.624 ± 0.022
0.548TrpThr: 0.548 ± 0.021
0.648TrpVal: 0.648 ± 0.021
0.15TrpTrp: 0.15 ± 0.011
0.523TrpTyr: 0.523 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.977TyrAla: 2.977 ± 0.045
0.742TyrCys: 0.742 ± 0.024
2.567TyrAsp: 2.567 ± 0.039
3.107TyrGlu: 3.107 ± 0.049
2.093TyrPhe: 2.093 ± 0.042
3.325TyrGly: 3.325 ± 0.052
0.926TyrHis: 0.926 ± 0.026
2.847TyrIle: 2.847 ± 0.045
1.842TyrLys: 1.842 ± 0.036
4.389TyrLeu: 4.389 ± 0.061
1.213TyrMet: 1.213 ± 0.03
1.744TyrAsn: 1.744 ± 0.039
1.818TyrPro: 1.818 ± 0.034
1.51TyrGln: 1.51 ± 0.03
2.737TyrArg: 2.737 ± 0.052
2.479TyrSer: 2.479 ± 0.045
2.276TyrThr: 2.276 ± 0.043
2.54TyrVal: 2.54 ± 0.037
0.491TyrTrp: 0.491 ± 0.018
2.142TyrTyr: 2.142 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4332 proteins (1520675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski