Amino acid dipepetide frequency for Escherichia phage vB_EcoS_XY1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.494AlaAla: 10.494 ± 1.43
0.791AlaCys: 0.791 ± 0.216
5.894AlaAsp: 5.894 ± 0.638
6.613AlaGlu: 6.613 ± 0.879
3.881AlaPhe: 3.881 ± 0.437
7.044AlaGly: 7.044 ± 0.714
1.366AlaHis: 1.366 ± 0.289
5.175AlaIle: 5.175 ± 0.773
5.966AlaLys: 5.966 ± 0.729
7.763AlaLeu: 7.763 ± 0.866
2.588AlaMet: 2.588 ± 0.45
3.809AlaAsn: 3.809 ± 0.431
3.953AlaPro: 3.953 ± 0.495
3.234AlaGln: 3.234 ± 0.742
4.888AlaArg: 4.888 ± 0.517
5.822AlaSer: 5.822 ± 0.833
5.822AlaThr: 5.822 ± 0.645
6.9AlaVal: 6.9 ± 0.663
1.581AlaTrp: 1.581 ± 0.361
3.234AlaTyr: 3.234 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.934CysAla: 0.934 ± 0.25
0.072CysCys: 0.072 ± 0.076
0.791CysAsp: 0.791 ± 0.3
0.863CysGlu: 0.863 ± 0.26
0.216CysPhe: 0.216 ± 0.131
0.863CysGly: 0.863 ± 0.208
0.288CysHis: 0.288 ± 0.148
0.719CysIle: 0.719 ± 0.214
0.934CysLys: 0.934 ± 0.283
0.791CysLeu: 0.791 ± 0.271
0.288CysMet: 0.288 ± 0.173
0.431CysAsn: 0.431 ± 0.194
0.144CysPro: 0.144 ± 0.104
0.288CysGln: 0.288 ± 0.128
0.503CysArg: 0.503 ± 0.196
0.359CysSer: 0.359 ± 0.169
0.719CysThr: 0.719 ± 0.27
0.863CysVal: 0.863 ± 0.276
0.216CysTrp: 0.216 ± 0.118
0.503CysTyr: 0.503 ± 0.215
0.0CysXaa: 0.0 ± 0.0
Asp
5.822AspAla: 5.822 ± 0.742
0.719AspCys: 0.719 ± 0.195
5.391AspAsp: 5.391 ± 0.597
4.313AspGlu: 4.313 ± 0.637
2.875AspPhe: 2.875 ± 0.442
6.828AspGly: 6.828 ± 1.059
0.647AspHis: 0.647 ± 0.179
4.241AspIle: 4.241 ± 0.414
3.091AspLys: 3.091 ± 0.384
4.744AspLeu: 4.744 ± 0.592
1.509AspMet: 1.509 ± 0.25
2.3AspAsn: 2.3 ± 0.454
1.222AspPro: 1.222 ± 0.34
1.078AspGln: 1.078 ± 0.299
2.444AspArg: 2.444 ± 0.437
2.947AspSer: 2.947 ± 0.391
3.666AspThr: 3.666 ± 0.516
4.744AspVal: 4.744 ± 0.588
0.934AspTrp: 0.934 ± 0.237
2.372AspTyr: 2.372 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
6.469GluAla: 6.469 ± 0.882
0.503GluCys: 0.503 ± 0.169
3.522GluAsp: 3.522 ± 0.41
5.391GluGlu: 5.391 ± 0.97
3.163GluPhe: 3.163 ± 0.52
4.672GluGly: 4.672 ± 0.632
0.934GluHis: 0.934 ± 0.255
3.091GluIle: 3.091 ± 0.578
4.241GluLys: 4.241 ± 0.633
5.966GluLeu: 5.966 ± 0.608
3.163GluMet: 3.163 ± 0.522
2.013GluAsn: 2.013 ± 0.387
2.013GluPro: 2.013 ± 0.376
3.594GluGln: 3.594 ± 0.908
3.45GluArg: 3.45 ± 0.585
2.947GluSer: 2.947 ± 0.51
3.234GluThr: 3.234 ± 0.425
5.463GluVal: 5.463 ± 0.659
1.294GluTrp: 1.294 ± 0.359
2.516GluTyr: 2.516 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
2.588PheAla: 2.588 ± 0.39
0.359PheCys: 0.359 ± 0.201
2.947PheAsp: 2.947 ± 0.476
2.588PheGlu: 2.588 ± 0.454
1.15PhePhe: 1.15 ± 0.23
3.234PheGly: 3.234 ± 0.406
0.288PheHis: 0.288 ± 0.14
2.803PheIle: 2.803 ± 0.509
2.444PheLys: 2.444 ± 0.428
2.444PheLeu: 2.444 ± 0.441
0.575PheMet: 0.575 ± 0.195
2.156PheAsn: 2.156 ± 0.366
1.366PhePro: 1.366 ± 0.342
1.294PheGln: 1.294 ± 0.296
2.156PheArg: 2.156 ± 0.32
2.947PheSer: 2.947 ± 0.476
3.091PheThr: 3.091 ± 0.461
2.731PheVal: 2.731 ± 0.4
0.719PheTrp: 0.719 ± 0.211
1.366PheTyr: 1.366 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
7.331GlyAla: 7.331 ± 0.778
0.791GlyCys: 0.791 ± 0.221
4.888GlyAsp: 4.888 ± 0.695
5.463GlyGlu: 5.463 ± 0.661
3.594GlyPhe: 3.594 ± 0.671
6.109GlyGly: 6.109 ± 0.584
1.653GlyHis: 1.653 ± 0.438
2.731GlyIle: 2.731 ± 0.421
4.384GlyLys: 4.384 ± 0.625
5.031GlyLeu: 5.031 ± 0.557
2.372GlyMet: 2.372 ± 0.516
3.594GlyAsn: 3.594 ± 0.541
1.869GlyPro: 1.869 ± 0.39
2.731GlyGln: 2.731 ± 0.396
3.809GlyArg: 3.809 ± 0.441
5.534GlySer: 5.534 ± 0.643
4.025GlyThr: 4.025 ± 0.521
6.109GlyVal: 6.109 ± 0.812
1.15GlyTrp: 1.15 ± 0.259
2.947GlyTyr: 2.947 ± 0.502
0.0GlyXaa: 0.0 ± 0.0
His
1.294HisAla: 1.294 ± 0.292
0.575HisCys: 0.575 ± 0.167
1.222HisAsp: 1.222 ± 0.27
0.719HisGlu: 0.719 ± 0.214
0.575HisPhe: 0.575 ± 0.229
1.581HisGly: 1.581 ± 0.359
1.078HisHis: 1.078 ± 0.281
0.863HisIle: 0.863 ± 0.261
1.366HisLys: 1.366 ± 0.292
1.222HisLeu: 1.222 ± 0.286
0.359HisMet: 0.359 ± 0.137
0.503HisAsn: 0.503 ± 0.213
0.934HisPro: 0.934 ± 0.31
0.791HisGln: 0.791 ± 0.198
0.503HisArg: 0.503 ± 0.161
0.719HisSer: 0.719 ± 0.195
0.719HisThr: 0.719 ± 0.264
1.438HisVal: 1.438 ± 0.374
0.072HisTrp: 0.072 ± 0.071
0.647HisTyr: 0.647 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
5.247IleAla: 5.247 ± 0.712
0.647IleCys: 0.647 ± 0.223
4.025IleAsp: 4.025 ± 0.523
3.306IleGlu: 3.306 ± 0.495
1.438IlePhe: 1.438 ± 0.342
3.019IleGly: 3.019 ± 0.494
0.719IleHis: 0.719 ± 0.237
3.019IleIle: 3.019 ± 0.407
3.091IleLys: 3.091 ± 0.4
2.947IleLeu: 2.947 ± 0.474
0.934IleMet: 0.934 ± 0.315
3.378IleAsn: 3.378 ± 0.623
2.731IlePro: 2.731 ± 0.421
1.438IleGln: 1.438 ± 0.373
2.372IleArg: 2.372 ± 0.382
3.809IleSer: 3.809 ± 0.475
4.384IleThr: 4.384 ± 0.676
4.384IleVal: 4.384 ± 0.491
0.791IleTrp: 0.791 ± 0.21
1.725IleTyr: 1.725 ± 0.523
0.0IleXaa: 0.0 ± 0.0
Lys
6.181LysAla: 6.181 ± 0.947
0.431LysCys: 0.431 ± 0.166
3.809LysAsp: 3.809 ± 0.468
3.666LysGlu: 3.666 ± 0.604
2.731LysPhe: 2.731 ± 0.441
3.234LysGly: 3.234 ± 0.411
1.366LysHis: 1.366 ± 0.285
1.797LysIle: 1.797 ± 0.275
3.019LysLys: 3.019 ± 0.517
4.313LysLeu: 4.313 ± 0.526
2.803LysMet: 2.803 ± 0.464
1.869LysAsn: 1.869 ± 0.397
2.588LysPro: 2.588 ± 0.411
1.294LysGln: 1.294 ± 0.277
3.953LysArg: 3.953 ± 0.481
3.091LysSer: 3.091 ± 0.654
3.666LysThr: 3.666 ± 0.513
4.672LysVal: 4.672 ± 0.571
0.791LysTrp: 0.791 ± 0.192
2.875LysTyr: 2.875 ± 0.408
0.0LysXaa: 0.0 ± 0.0
Leu
8.266LeuAla: 8.266 ± 0.698
1.078LeuCys: 1.078 ± 0.285
3.881LeuAsp: 3.881 ± 0.521
5.031LeuGlu: 5.031 ± 0.66
1.869LeuPhe: 1.869 ± 0.387
4.888LeuGly: 4.888 ± 0.517
1.078LeuHis: 1.078 ± 0.241
4.528LeuIle: 4.528 ± 0.402
4.313LeuLys: 4.313 ± 0.635
5.175LeuLeu: 5.175 ± 0.602
1.725LeuMet: 1.725 ± 0.274
3.594LeuAsn: 3.594 ± 0.544
3.45LeuPro: 3.45 ± 0.583
2.3LeuGln: 2.3 ± 0.481
4.959LeuArg: 4.959 ± 0.604
3.522LeuSer: 3.522 ± 0.467
5.463LeuThr: 5.463 ± 0.616
4.169LeuVal: 4.169 ± 0.555
1.222LeuTrp: 1.222 ± 0.305
2.659LeuTyr: 2.659 ± 0.521
0.0LeuXaa: 0.0 ± 0.0
Met
2.803MetAla: 2.803 ± 0.459
0.431MetCys: 0.431 ± 0.136
0.719MetAsp: 0.719 ± 0.242
1.15MetGlu: 1.15 ± 0.35
1.294MetPhe: 1.294 ± 0.265
1.725MetGly: 1.725 ± 0.313
0.503MetHis: 0.503 ± 0.213
1.725MetIle: 1.725 ± 0.312
1.653MetLys: 1.653 ± 0.379
1.869MetLeu: 1.869 ± 0.368
0.575MetMet: 0.575 ± 0.212
1.006MetAsn: 1.006 ± 0.286
1.509MetPro: 1.509 ± 0.327
0.575MetGln: 0.575 ± 0.216
1.509MetArg: 1.509 ± 0.259
2.372MetSer: 2.372 ± 0.285
2.084MetThr: 2.084 ± 0.379
1.725MetVal: 1.725 ± 0.259
0.288MetTrp: 0.288 ± 0.138
0.863MetTyr: 0.863 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
3.809AsnAla: 3.809 ± 0.533
0.431AsnCys: 0.431 ± 0.179
2.372AsnAsp: 2.372 ± 0.399
2.3AsnGlu: 2.3 ± 0.368
1.366AsnPhe: 1.366 ± 0.307
4.384AsnGly: 4.384 ± 0.498
0.647AsnHis: 0.647 ± 0.242
2.084AsnIle: 2.084 ± 0.46
2.013AsnLys: 2.013 ± 0.38
2.731AsnLeu: 2.731 ± 0.357
1.006AsnMet: 1.006 ± 0.332
2.084AsnAsn: 2.084 ± 0.379
2.084AsnPro: 2.084 ± 0.387
1.366AsnGln: 1.366 ± 0.386
2.3AsnArg: 2.3 ± 0.415
2.516AsnSer: 2.516 ± 0.3
2.516AsnThr: 2.516 ± 0.369
4.097AsnVal: 4.097 ± 0.439
0.791AsnTrp: 0.791 ± 0.271
1.797AsnTyr: 1.797 ± 0.325
0.0AsnXaa: 0.0 ± 0.0
Pro
3.234ProAla: 3.234 ± 0.38
0.503ProCys: 0.503 ± 0.168
2.372ProAsp: 2.372 ± 0.398
4.097ProGlu: 4.097 ± 0.588
1.797ProPhe: 1.797 ± 0.361
2.947ProGly: 2.947 ± 0.453
0.431ProHis: 0.431 ± 0.166
1.797ProIle: 1.797 ± 0.375
1.509ProLys: 1.509 ± 0.442
3.019ProLeu: 3.019 ± 0.528
0.647ProMet: 0.647 ± 0.265
1.581ProAsn: 1.581 ± 0.453
1.078ProPro: 1.078 ± 0.257
1.294ProGln: 1.294 ± 0.252
2.084ProArg: 2.084 ± 0.375
1.797ProSer: 1.797 ± 0.332
2.444ProThr: 2.444 ± 0.367
3.666ProVal: 3.666 ± 0.404
0.359ProTrp: 0.359 ± 0.151
1.366ProTyr: 1.366 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
3.306GlnAla: 3.306 ± 0.536
0.288GlnCys: 0.288 ± 0.165
1.941GlnAsp: 1.941 ± 0.371
2.372GlnGlu: 2.372 ± 0.542
1.15GlnPhe: 1.15 ± 0.271
1.725GlnGly: 1.725 ± 0.395
0.863GlnHis: 0.863 ± 0.274
1.941GlnIle: 1.941 ± 0.35
1.725GlnLys: 1.725 ± 0.368
2.659GlnLeu: 2.659 ± 0.502
1.222GlnMet: 1.222 ± 0.241
1.725GlnAsn: 1.725 ± 0.433
1.438GlnPro: 1.438 ± 0.31
2.3GlnGln: 2.3 ± 0.569
2.444GlnArg: 2.444 ± 0.392
1.581GlnSer: 1.581 ± 0.314
1.797GlnThr: 1.797 ± 0.461
2.228GlnVal: 2.228 ± 0.381
0.791GlnTrp: 0.791 ± 0.214
1.509GlnTyr: 1.509 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
4.241ArgAla: 4.241 ± 0.57
0.647ArgCys: 0.647 ± 0.257
2.659ArgAsp: 2.659 ± 0.318
3.953ArgGlu: 3.953 ± 0.502
2.013ArgPhe: 2.013 ± 0.301
3.666ArgGly: 3.666 ± 0.516
1.006ArgHis: 1.006 ± 0.267
3.019ArgIle: 3.019 ± 0.456
4.241ArgLys: 4.241 ± 0.633
4.169ArgLeu: 4.169 ± 0.371
1.653ArgMet: 1.653 ± 0.256
2.731ArgAsn: 2.731 ± 0.418
1.797ArgPro: 1.797 ± 0.328
2.803ArgGln: 2.803 ± 0.439
4.528ArgArg: 4.528 ± 0.695
2.588ArgSer: 2.588 ± 0.373
2.731ArgThr: 2.731 ± 0.358
3.378ArgVal: 3.378 ± 0.372
1.006ArgTrp: 1.006 ± 0.324
1.653ArgTyr: 1.653 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
5.534SerAla: 5.534 ± 0.791
0.575SerCys: 0.575 ± 0.199
3.594SerAsp: 3.594 ± 0.487
2.947SerGlu: 2.947 ± 0.417
2.731SerPhe: 2.731 ± 0.423
5.966SerGly: 5.966 ± 0.503
0.934SerHis: 0.934 ± 0.219
2.947SerIle: 2.947 ± 0.591
3.234SerLys: 3.234 ± 0.418
3.953SerLeu: 3.953 ± 0.525
1.006SerMet: 1.006 ± 0.218
3.306SerAsn: 3.306 ± 0.494
2.659SerPro: 2.659 ± 0.53
1.725SerGln: 1.725 ± 0.349
3.234SerArg: 3.234 ± 0.418
3.522SerSer: 3.522 ± 0.554
4.169SerThr: 4.169 ± 0.503
4.384SerVal: 4.384 ± 0.674
0.503SerTrp: 0.503 ± 0.161
2.372SerTyr: 2.372 ± 0.501
0.0SerXaa: 0.0 ± 0.0
Thr
7.044ThrAla: 7.044 ± 0.625
0.575ThrCys: 0.575 ± 0.221
3.953ThrAsp: 3.953 ± 0.525
3.809ThrGlu: 3.809 ± 0.446
2.731ThrPhe: 2.731 ± 0.471
5.463ThrGly: 5.463 ± 0.715
1.006ThrHis: 1.006 ± 0.313
3.953ThrIle: 3.953 ± 0.469
3.019ThrLys: 3.019 ± 0.477
4.959ThrLeu: 4.959 ± 0.516
1.078ThrMet: 1.078 ± 0.304
1.725ThrAsn: 1.725 ± 0.374
4.025ThrPro: 4.025 ± 0.533
1.941ThrGln: 1.941 ± 0.274
2.803ThrArg: 2.803 ± 0.506
3.163ThrSer: 3.163 ± 0.531
4.025ThrThr: 4.025 ± 0.669
3.738ThrVal: 3.738 ± 0.529
1.222ThrTrp: 1.222 ± 0.323
2.588ThrTyr: 2.588 ± 0.521
0.0ThrXaa: 0.0 ± 0.0
Val
7.547ValAla: 7.547 ± 0.813
0.719ValCys: 0.719 ± 0.235
4.097ValAsp: 4.097 ± 0.604
5.391ValGlu: 5.391 ± 0.814
2.444ValPhe: 2.444 ± 0.388
4.384ValGly: 4.384 ± 0.442
0.934ValHis: 0.934 ± 0.224
4.456ValIle: 4.456 ± 0.513
4.313ValLys: 4.313 ± 0.465
4.959ValLeu: 4.959 ± 0.605
1.725ValMet: 1.725 ± 0.376
2.659ValAsn: 2.659 ± 0.459
1.653ValPro: 1.653 ± 0.371
2.947ValGln: 2.947 ± 0.546
3.738ValArg: 3.738 ± 0.504
6.109ValSer: 6.109 ± 0.722
5.75ValThr: 5.75 ± 0.74
5.391ValVal: 5.391 ± 0.642
1.006ValTrp: 1.006 ± 0.263
3.234ValTyr: 3.234 ± 0.503
0.0ValXaa: 0.0 ± 0.0
Trp
1.078TrpAla: 1.078 ± 0.431
0.288TrpCys: 0.288 ± 0.127
0.575TrpAsp: 0.575 ± 0.156
0.791TrpGlu: 0.791 ± 0.258
0.863TrpPhe: 0.863 ± 0.264
1.366TrpGly: 1.366 ± 0.302
0.575TrpHis: 0.575 ± 0.209
0.431TrpIle: 0.431 ± 0.182
0.934TrpLys: 0.934 ± 0.262
2.013TrpLeu: 2.013 ± 0.357
0.503TrpMet: 0.503 ± 0.187
0.934TrpAsn: 0.934 ± 0.267
0.288TrpPro: 0.288 ± 0.157
0.575TrpGln: 0.575 ± 0.236
0.719TrpArg: 0.719 ± 0.21
1.15TrpSer: 1.15 ± 0.339
0.575TrpThr: 0.575 ± 0.193
1.006TrpVal: 1.006 ± 0.223
0.144TrpTrp: 0.144 ± 0.1
0.503TrpTyr: 0.503 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.594TyrAla: 3.594 ± 0.536
0.359TyrCys: 0.359 ± 0.159
3.234TyrAsp: 3.234 ± 0.74
2.875TyrGlu: 2.875 ± 0.379
1.366TyrPhe: 1.366 ± 0.328
3.019TyrGly: 3.019 ± 0.391
0.863TyrHis: 0.863 ± 0.22
2.156TyrIle: 2.156 ± 0.345
2.659TyrLys: 2.659 ± 0.394
2.444TyrLeu: 2.444 ± 0.419
0.575TyrMet: 0.575 ± 0.189
1.294TyrAsn: 1.294 ± 0.294
1.222TyrPro: 1.222 ± 0.308
1.438TyrGln: 1.438 ± 0.268
2.084TyrArg: 2.084 ± 0.353
2.803TyrSer: 2.803 ± 0.493
1.941TyrThr: 1.941 ± 0.384
2.372TyrVal: 2.372 ± 0.39
0.431TyrTrp: 0.431 ± 0.195
1.438TyrTyr: 1.438 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (13914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski