Microbacterium phage Alakazam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; unclassified Neferthenavirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R0X6|A0A6G8R0X6_9CAUD MazG-like nucleotide pyrophosphohydrolase OS=Microbacterium phage Alakazam OX=2713265 GN=43 PE=4 SV=1
MM1 pKa = 7.25TAIDD5 pKa = 4.72DD6 pKa = 4.4LNGLLEE12 pKa = 4.57PLPGYY17 pKa = 10.61AVLTPAMKK25 pKa = 10.47QNALNMALIPDD36 pKa = 4.45AAGVWPGQPGYY47 pKa = 9.3VTTYY51 pKa = 10.27DD52 pKa = 3.85IYY54 pKa = 10.38WAAISLTGYY63 pKa = 10.06MMAQPFVKK71 pKa = 10.18QAASEE76 pKa = 4.48GTSTTVDD83 pKa = 3.08APNWSGILAYY93 pKa = 10.19FRR95 pKa = 11.84SQSVIAQATATGPILNEE112 pKa = 3.84VPIPGLPHH120 pKa = 6.39VVPTDD125 pKa = 3.29MSGYY129 pKa = 10.61GGDD132 pKa = 3.81GYY134 pKa = 11.64GDD136 pKa = 3.73VDD138 pKa = 3.56TDD140 pKa = 3.99LAA142 pKa = 5.6

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R112|A0A6G8R112_9CAUD Uncharacterized protein OS=Microbacterium phage Alakazam OX=2713265 GN=55 PE=4 SV=1
MM1 pKa = 6.69NTAAPITSLRR11 pKa = 11.84QLTDD15 pKa = 3.29AQRR18 pKa = 11.84HH19 pKa = 5.53AIISLGSVMKK29 pKa = 10.77YY30 pKa = 7.91RR31 pKa = 11.84TATEE35 pKa = 4.39LEE37 pKa = 4.12LSGRR41 pKa = 11.84TLVSLVEE48 pKa = 4.19LGLVEE53 pKa = 4.12MKK55 pKa = 10.2SYY57 pKa = 10.67RR58 pKa = 11.84VQSSNPRR65 pKa = 11.84ARR67 pKa = 11.84VSNEE71 pKa = 3.51PIIRR75 pKa = 11.84TYY77 pKa = 10.09RR78 pKa = 11.84NSMAGYY84 pKa = 8.34RR85 pKa = 11.84LARR88 pKa = 11.84KK89 pKa = 9.37GLL91 pKa = 3.61

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13361

37

811

222.7

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.591 ± 0.464

0.606 ± 0.078

6.362 ± 0.358

6.279 ± 0.47

2.837 ± 0.172

7.919 ± 0.413

1.766 ± 0.194

5.217 ± 0.353

4.85 ± 0.327

8.45 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.162

3.293 ± 0.223

5.306 ± 0.245

4.236 ± 0.224

5.838 ± 0.429

5.292 ± 0.357

6.631 ± 0.416

7.372 ± 0.323

1.924 ± 0.18

2.882 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski