Amino acid dipepetide frequency for Microbacterium phage Alakazam

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.874AlaAla: 12.874 ± 1.588
0.599AlaCys: 0.599 ± 0.203
5.689AlaAsp: 5.689 ± 0.78
6.138AlaGlu: 6.138 ± 0.656
3.293AlaPhe: 3.293 ± 0.414
7.784AlaGly: 7.784 ± 1.05
1.198AlaHis: 1.198 ± 0.327
6.287AlaIle: 6.287 ± 0.841
6.287AlaLys: 6.287 ± 0.809
9.281AlaLeu: 9.281 ± 0.94
2.919AlaMet: 2.919 ± 0.464
3.817AlaAsn: 3.817 ± 0.636
4.566AlaPro: 4.566 ± 0.828
4.716AlaGln: 4.716 ± 0.686
7.41AlaArg: 7.41 ± 1.048
5.165AlaSer: 5.165 ± 0.581
6.587AlaThr: 6.587 ± 0.679
7.26AlaVal: 7.26 ± 0.672
1.796AlaTrp: 1.796 ± 0.349
2.246AlaTyr: 2.246 ± 0.43
0.0AlaXaa: 0.0 ± 0.0
Cys
0.449CysAla: 0.449 ± 0.162
0.0CysCys: 0.0 ± 0.0
0.524CysAsp: 0.524 ± 0.154
0.299CysGlu: 0.299 ± 0.156
0.15CysPhe: 0.15 ± 0.113
0.599CysGly: 0.599 ± 0.228
0.15CysHis: 0.15 ± 0.112
0.299CysIle: 0.299 ± 0.14
0.374CysLys: 0.374 ± 0.158
0.075CysLeu: 0.075 ± 0.066
0.0CysMet: 0.0 ± 0.0
0.15CysAsn: 0.15 ± 0.1
0.823CysPro: 0.823 ± 0.248
0.299CysGln: 0.299 ± 0.209
0.449CysArg: 0.449 ± 0.171
0.15CysSer: 0.15 ± 0.091
0.449CysThr: 0.449 ± 0.187
0.449CysVal: 0.449 ± 0.205
0.225CysTrp: 0.225 ± 0.127
0.15CysTyr: 0.15 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
5.389AspAla: 5.389 ± 0.835
0.524AspCys: 0.524 ± 0.191
4.94AspAsp: 4.94 ± 1.086
5.389AspGlu: 5.389 ± 1.369
2.021AspPhe: 2.021 ± 0.391
5.689AspGly: 5.689 ± 0.754
1.272AspHis: 1.272 ± 0.275
2.545AspIle: 2.545 ± 0.42
2.395AspLys: 2.395 ± 0.349
5.913AspLeu: 5.913 ± 0.61
1.272AspMet: 1.272 ± 0.285
1.272AspAsn: 1.272 ± 0.326
4.042AspPro: 4.042 ± 0.507
2.395AspGln: 2.395 ± 0.458
3.892AspArg: 3.892 ± 0.502
2.769AspSer: 2.769 ± 0.387
4.491AspThr: 4.491 ± 0.649
3.668AspVal: 3.668 ± 0.479
1.422AspTrp: 1.422 ± 0.384
2.32AspTyr: 2.32 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
7.111GluAla: 7.111 ± 1.0
0.449GluCys: 0.449 ± 0.164
5.464GluAsp: 5.464 ± 0.992
5.24GluGlu: 5.24 ± 1.127
1.722GluPhe: 1.722 ± 0.398
4.716GluGly: 4.716 ± 0.641
0.973GluHis: 0.973 ± 0.278
2.47GluIle: 2.47 ± 0.418
2.395GluLys: 2.395 ± 0.384
7.186GluLeu: 7.186 ± 0.715
1.347GluMet: 1.347 ± 0.321
1.198GluAsn: 1.198 ± 0.322
2.695GluPro: 2.695 ± 0.455
2.096GluGln: 2.096 ± 0.387
4.042GluArg: 4.042 ± 0.582
2.47GluSer: 2.47 ± 0.347
3.293GluThr: 3.293 ± 0.546
4.716GluVal: 4.716 ± 0.576
1.347GluTrp: 1.347 ± 0.389
1.871GluTyr: 1.871 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
2.62PheAla: 2.62 ± 0.386
0.374PheCys: 0.374 ± 0.179
1.347PheAsp: 1.347 ± 0.294
1.347PheGlu: 1.347 ± 0.313
0.449PhePhe: 0.449 ± 0.159
2.919PheGly: 2.919 ± 0.597
0.599PheHis: 0.599 ± 0.238
1.647PheIle: 1.647 ± 0.306
1.572PheLys: 1.572 ± 0.311
2.395PheLeu: 2.395 ± 0.319
0.599PheMet: 0.599 ± 0.222
1.198PheAsn: 1.198 ± 0.345
1.272PhePro: 1.272 ± 0.236
1.272PheGln: 1.272 ± 0.342
1.796PheArg: 1.796 ± 0.362
1.572PheSer: 1.572 ± 0.434
2.695PheThr: 2.695 ± 0.467
1.722PheVal: 1.722 ± 0.358
0.449PheTrp: 0.449 ± 0.165
0.524PheTyr: 0.524 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
7.036GlyAla: 7.036 ± 1.025
0.449GlyCys: 0.449 ± 0.182
4.491GlyAsp: 4.491 ± 0.538
3.892GlyGlu: 3.892 ± 0.561
2.844GlyPhe: 2.844 ± 0.436
6.138GlyGly: 6.138 ± 0.923
1.272GlyHis: 1.272 ± 0.293
3.967GlyIle: 3.967 ± 0.721
5.689GlyLys: 5.689 ± 0.699
6.362GlyLeu: 6.362 ± 0.931
1.946GlyMet: 1.946 ± 0.388
1.572GlyAsn: 1.572 ± 0.326
3.743GlyPro: 3.743 ± 1.188
3.817GlyGln: 3.817 ± 0.603
5.09GlyArg: 5.09 ± 0.674
4.716GlySer: 4.716 ± 0.863
6.437GlyThr: 6.437 ± 0.654
5.614GlyVal: 5.614 ± 0.653
1.198GlyTrp: 1.198 ± 0.273
2.919GlyTyr: 2.919 ± 0.5
0.0GlyXaa: 0.0 ± 0.0
His
1.347HisAla: 1.347 ± 0.343
0.0HisCys: 0.0 ± 0.0
0.749HisAsp: 0.749 ± 0.237
1.272HisGlu: 1.272 ± 0.24
0.898HisPhe: 0.898 ± 0.279
1.497HisGly: 1.497 ± 0.334
0.299HisHis: 0.299 ± 0.18
1.123HisIle: 1.123 ± 0.269
1.123HisLys: 1.123 ± 0.264
1.422HisLeu: 1.422 ± 0.395
0.374HisMet: 0.374 ± 0.153
0.299HisAsn: 0.299 ± 0.171
1.048HisPro: 1.048 ± 0.296
0.973HisGln: 0.973 ± 0.338
0.973HisArg: 0.973 ± 0.257
0.449HisSer: 0.449 ± 0.174
1.272HisThr: 1.272 ± 0.364
1.198HisVal: 1.198 ± 0.309
0.374HisTrp: 0.374 ± 0.158
0.973HisTyr: 0.973 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
5.689IleAla: 5.689 ± 0.618
0.15IleCys: 0.15 ± 0.115
3.219IleAsp: 3.219 ± 0.425
3.368IleGlu: 3.368 ± 0.443
1.123IlePhe: 1.123 ± 0.337
3.668IleGly: 3.668 ± 0.646
0.973IleHis: 0.973 ± 0.207
3.967IleIle: 3.967 ± 0.717
2.246IleLys: 2.246 ± 0.504
3.293IleLeu: 3.293 ± 0.52
1.123IleMet: 1.123 ± 0.276
2.096IleAsn: 2.096 ± 0.366
3.518IlePro: 3.518 ± 0.701
2.919IleGln: 2.919 ± 0.654
2.545IleArg: 2.545 ± 0.476
2.47IleSer: 2.47 ± 0.558
4.117IleThr: 4.117 ± 0.551
3.518IleVal: 3.518 ± 0.586
0.823IleTrp: 0.823 ± 0.23
1.347IleTyr: 1.347 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
5.614LysAla: 5.614 ± 0.935
0.225LysCys: 0.225 ± 0.124
2.62LysAsp: 2.62 ± 0.488
3.668LysGlu: 3.668 ± 0.533
1.048LysPhe: 1.048 ± 0.271
3.892LysGly: 3.892 ± 0.472
0.898LysHis: 0.898 ± 0.245
2.096LysIle: 2.096 ± 0.34
1.647LysLys: 1.647 ± 0.397
3.967LysLeu: 3.967 ± 0.601
1.572LysMet: 1.572 ± 0.349
1.347LysAsn: 1.347 ± 0.26
3.967LysPro: 3.967 ± 0.619
1.871LysGln: 1.871 ± 0.409
2.32LysArg: 2.32 ± 0.414
1.946LysSer: 1.946 ± 0.329
3.293LysThr: 3.293 ± 0.447
3.967LysVal: 3.967 ± 0.43
1.347LysTrp: 1.347 ± 0.328
1.198LysTyr: 1.198 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
10.928LeuAla: 10.928 ± 0.918
0.449LeuCys: 0.449 ± 0.157
6.063LeuAsp: 6.063 ± 0.617
4.865LeuGlu: 4.865 ± 0.598
2.096LeuPhe: 2.096 ± 0.451
6.138LeuGly: 6.138 ± 0.736
1.198LeuHis: 1.198 ± 0.319
5.389LeuIle: 5.389 ± 0.926
3.593LeuLys: 3.593 ± 0.548
6.138LeuLeu: 6.138 ± 0.59
1.497LeuMet: 1.497 ± 0.365
4.266LeuAsn: 4.266 ± 0.536
5.314LeuPro: 5.314 ± 0.789
2.47LeuGln: 2.47 ± 0.436
5.015LeuArg: 5.015 ± 0.805
3.967LeuSer: 3.967 ± 0.549
5.464LeuThr: 5.464 ± 0.548
6.362LeuVal: 6.362 ± 0.986
1.198LeuTrp: 1.198 ± 0.268
2.096LeuTyr: 2.096 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
2.545MetAla: 2.545 ± 0.399
0.299MetCys: 0.299 ± 0.133
1.647MetAsp: 1.647 ± 0.32
1.647MetGlu: 1.647 ± 0.352
0.749MetPhe: 0.749 ± 0.263
2.096MetGly: 2.096 ± 0.359
0.225MetHis: 0.225 ± 0.125
1.048MetIle: 1.048 ± 0.33
1.422MetLys: 1.422 ± 0.292
1.796MetLeu: 1.796 ± 0.419
0.599MetMet: 0.599 ± 0.194
0.449MetAsn: 0.449 ± 0.177
1.497MetPro: 1.497 ± 0.313
0.374MetGln: 0.374 ± 0.169
1.198MetArg: 1.198 ± 0.313
1.647MetSer: 1.647 ± 0.34
1.497MetThr: 1.497 ± 0.332
1.796MetVal: 1.796 ± 0.394
0.299MetTrp: 0.299 ± 0.142
0.674MetTyr: 0.674 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
3.967AsnAla: 3.967 ± 0.645
0.075AsnCys: 0.075 ± 0.074
2.096AsnAsp: 2.096 ± 0.344
1.647AsnGlu: 1.647 ± 0.373
1.272AsnPhe: 1.272 ± 0.275
3.144AsnGly: 3.144 ± 0.491
0.674AsnHis: 0.674 ± 0.203
1.198AsnIle: 1.198 ± 0.277
1.272AsnLys: 1.272 ± 0.235
2.395AsnLeu: 2.395 ± 0.476
0.599AsnMet: 0.599 ± 0.158
1.347AsnAsn: 1.347 ± 0.381
1.871AsnPro: 1.871 ± 0.39
0.973AsnGln: 0.973 ± 0.284
1.647AsnArg: 1.647 ± 0.326
2.395AsnSer: 2.395 ± 0.46
2.171AsnThr: 2.171 ± 0.426
1.946AsnVal: 1.946 ± 0.396
0.823AsnTrp: 0.823 ± 0.263
1.422AsnTyr: 1.422 ± 0.352
0.0AsnXaa: 0.0 ± 0.0
Pro
6.662ProAla: 6.662 ± 0.856
0.225ProCys: 0.225 ± 0.122
4.117ProAsp: 4.117 ± 0.696
3.593ProGlu: 3.593 ± 0.689
1.048ProPhe: 1.048 ± 0.235
5.09ProGly: 5.09 ± 1.058
0.823ProHis: 0.823 ± 0.222
2.096ProIle: 2.096 ± 0.436
2.919ProLys: 2.919 ± 0.494
3.443ProLeu: 3.443 ± 0.551
1.123ProMet: 1.123 ± 0.326
1.347ProAsn: 1.347 ± 0.303
1.422ProPro: 1.422 ± 0.305
2.545ProGln: 2.545 ± 0.721
2.994ProArg: 2.994 ± 0.534
2.62ProSer: 2.62 ± 0.418
4.266ProThr: 4.266 ± 0.514
4.341ProVal: 4.341 ± 0.515
0.823ProTrp: 0.823 ± 0.247
1.572ProTyr: 1.572 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
5.165GlnAla: 5.165 ± 0.503
0.15GlnCys: 0.15 ± 0.091
2.395GlnAsp: 2.395 ± 0.386
2.994GlnGlu: 2.994 ± 0.411
0.674GlnPhe: 0.674 ± 0.273
3.593GlnGly: 3.593 ± 0.614
1.048GlnHis: 1.048 ± 0.316
1.796GlnIle: 1.796 ± 0.537
1.946GlnLys: 1.946 ± 0.354
3.743GlnLeu: 3.743 ± 0.583
0.823GlnMet: 0.823 ± 0.212
1.572GlnAsn: 1.572 ± 0.351
2.021GlnPro: 2.021 ± 0.398
2.62GlnGln: 2.62 ± 0.707
2.769GlnArg: 2.769 ± 0.457
2.47GlnSer: 2.47 ± 0.498
1.722GlnThr: 1.722 ± 0.375
2.994GlnVal: 2.994 ± 0.466
0.898GlnTrp: 0.898 ± 0.294
0.973GlnTyr: 0.973 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
5.838ArgAla: 5.838 ± 0.869
0.674ArgCys: 0.674 ± 0.253
3.368ArgAsp: 3.368 ± 0.493
3.593ArgGlu: 3.593 ± 0.614
2.096ArgPhe: 2.096 ± 0.382
4.117ArgGly: 4.117 ± 0.743
1.048ArgHis: 1.048 ± 0.316
2.994ArgIle: 2.994 ± 0.577
3.293ArgLys: 3.293 ± 0.607
4.79ArgLeu: 4.79 ± 0.606
2.32ArgMet: 2.32 ± 0.4
1.946ArgAsn: 1.946 ± 0.385
2.919ArgPro: 2.919 ± 0.498
2.695ArgGln: 2.695 ± 0.421
3.069ArgArg: 3.069 ± 0.578
2.844ArgSer: 2.844 ± 0.439
3.593ArgThr: 3.593 ± 0.504
5.389ArgVal: 5.389 ± 0.572
1.048ArgTrp: 1.048 ± 0.311
0.749ArgTyr: 0.749 ± 0.253
0.0ArgXaa: 0.0 ± 0.0
Ser
6.287SerAla: 6.287 ± 0.826
0.15SerCys: 0.15 ± 0.103
3.368SerAsp: 3.368 ± 0.372
2.545SerGlu: 2.545 ± 0.526
1.123SerPhe: 1.123 ± 0.311
3.743SerGly: 3.743 ± 0.553
0.674SerHis: 0.674 ± 0.229
2.62SerIle: 2.62 ± 0.528
2.62SerLys: 2.62 ± 0.439
5.464SerLeu: 5.464 ± 0.747
1.572SerMet: 1.572 ± 0.333
1.647SerAsn: 1.647 ± 0.536
2.47SerPro: 2.47 ± 0.312
2.32SerGln: 2.32 ± 0.333
2.021SerArg: 2.021 ± 0.394
3.144SerSer: 3.144 ± 0.552
3.368SerThr: 3.368 ± 0.542
4.042SerVal: 4.042 ± 0.864
1.272SerTrp: 1.272 ± 0.353
2.47SerTyr: 2.47 ± 0.483
0.0SerXaa: 0.0 ± 0.0
Thr
5.464ThrAla: 5.464 ± 0.844
0.374ThrCys: 0.374 ± 0.211
2.919ThrAsp: 2.919 ± 0.448
3.443ThrGlu: 3.443 ± 0.515
2.47ThrPhe: 2.47 ± 0.64
5.988ThrGly: 5.988 ± 0.671
1.422ThrHis: 1.422 ± 0.349
3.743ThrIle: 3.743 ± 0.664
2.844ThrLys: 2.844 ± 0.459
6.138ThrLeu: 6.138 ± 0.604
1.048ThrMet: 1.048 ± 0.243
2.021ThrAsn: 2.021 ± 0.393
3.443ThrPro: 3.443 ± 0.457
2.32ThrGln: 2.32 ± 0.433
4.266ThrArg: 4.266 ± 0.5
4.341ThrSer: 4.341 ± 0.574
4.266ThrThr: 4.266 ± 1.069
6.437ThrVal: 6.437 ± 0.79
1.422ThrTrp: 1.422 ± 0.311
1.946ThrTyr: 1.946 ± 0.421
0.0ThrXaa: 0.0 ± 0.0
Val
7.41ValAla: 7.41 ± 0.711
0.374ValCys: 0.374 ± 0.142
5.015ValAsp: 5.015 ± 0.545
4.94ValGlu: 4.94 ± 0.648
1.871ValPhe: 1.871 ± 0.384
5.314ValGly: 5.314 ± 0.571
1.796ValHis: 1.796 ± 0.422
4.042ValIle: 4.042 ± 0.607
3.293ValLys: 3.293 ± 0.469
6.811ValLeu: 6.811 ± 0.677
1.422ValMet: 1.422 ± 0.34
2.545ValAsn: 2.545 ± 0.595
3.668ValPro: 3.668 ± 0.542
3.743ValGln: 3.743 ± 0.64
3.817ValArg: 3.817 ± 0.563
4.79ValSer: 4.79 ± 0.526
4.416ValThr: 4.416 ± 0.594
4.865ValVal: 4.865 ± 0.568
1.497ValTrp: 1.497 ± 0.354
2.021ValTyr: 2.021 ± 0.529
0.0ValXaa: 0.0 ± 0.0
Trp
1.123TrpAla: 1.123 ± 0.271
0.15TrpCys: 0.15 ± 0.088
1.647TrpAsp: 1.647 ± 0.262
1.123TrpGlu: 1.123 ± 0.267
0.749TrpPhe: 0.749 ± 0.257
0.823TrpGly: 0.823 ± 0.253
0.524TrpHis: 0.524 ± 0.175
1.497TrpIle: 1.497 ± 0.361
0.599TrpLys: 0.599 ± 0.197
1.722TrpLeu: 1.722 ± 0.389
0.599TrpMet: 0.599 ± 0.229
1.272TrpAsn: 1.272 ± 0.279
1.048TrpPro: 1.048 ± 0.276
0.973TrpGln: 0.973 ± 0.266
0.823TrpArg: 0.823 ± 0.206
0.973TrpSer: 0.973 ± 0.306
1.572TrpThr: 1.572 ± 0.309
0.973TrpVal: 0.973 ± 0.266
0.524TrpTrp: 0.524 ± 0.199
0.524TrpTyr: 0.524 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.395TyrAla: 2.395 ± 0.373
0.374TyrCys: 0.374 ± 0.178
1.946TyrAsp: 1.946 ± 0.465
1.796TyrGlu: 1.796 ± 0.47
0.674TyrPhe: 0.674 ± 0.253
2.246TyrGly: 2.246 ± 0.373
0.599TyrHis: 0.599 ± 0.178
1.347TyrIle: 1.347 ± 0.257
0.973TyrLys: 0.973 ± 0.297
2.171TyrLeu: 2.171 ± 0.407
0.674TyrMet: 0.674 ± 0.183
1.572TyrAsn: 1.572 ± 0.318
1.722TyrPro: 1.722 ± 0.366
0.973TyrGln: 0.973 ± 0.271
2.246TyrArg: 2.246 ± 0.463
2.021TyrSer: 2.021 ± 0.462
1.347TyrThr: 1.347 ± 0.419
2.47TyrVal: 2.47 ± 0.539
0.449TyrTrp: 0.449 ± 0.208
0.823TyrTyr: 0.823 ± 0.285
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13361 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski