Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon nigroviridis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4RCK9|Q4RCK9_TETNG Chromosome undetermined SCAF18655 whole genome shotgun sequence (Fragment) OS=Tetraodon nigroviridis OX=99883 GN=GSTENG00036614001 PE=4 SV=1
MM1 pKa = 7.46SSSEE5 pKa = 4.12EE6 pKa = 3.97VSWISWFCGLRR17 pKa = 11.84GNEE20 pKa = 4.08FFCEE24 pKa = 3.77VDD26 pKa = 2.87EE27 pKa = 7.16DD28 pKa = 4.72YY29 pKa = 11.26IQDD32 pKa = 3.79KK33 pKa = 10.97FNLTGLNEE41 pKa = 4.25QVPHH45 pKa = 5.61YY46 pKa = 10.09RR47 pKa = 11.84QALDD51 pKa = 3.81MILDD55 pKa = 4.1LEE57 pKa = 4.47PP58 pKa = 5.28

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3C506|H3C506_TETNG BRICK1 subunit of SCAR/WAVE actin nucleating complex OS=Tetraodon nigroviridis OX=99883 GN=BRK1 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19570

3504

23074

12616183

16

18947

546.8

60.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.963 ± 0.016

2.328 ± 0.014

5.082 ± 0.011

6.577 ± 0.018

3.762 ± 0.011

6.581 ± 0.028

2.618 ± 0.009

4.272 ± 0.012

5.366 ± 0.016

9.874 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.007

3.676 ± 0.009

5.741 ± 0.021

4.68 ± 0.014

5.902 ± 0.012

8.448 ± 0.019

5.36 ± 0.01

6.52 ± 0.014

1.216 ± 0.005

2.768 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski