Amino acid dipepetide frequency for Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon nigroviridis)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.971AlaAla: 6.971 ± 0.041	1.437AlaCys: 1.437 ± 0.015	3.437AlaAsp: 3.437 ± 0.02	4.794AlaGlu: 4.794 ± 0.03	2.566AlaPhe: 2.566 ± 0.016	4.94AlaGly: 4.94 ± 0.039	1.598AlaHis: 1.598 ± 0.011	2.725AlaIle: 2.725 ± 0.016	3.364AlaLys: 3.364 ± 0.019	6.918AlaLeu: 6.918 ± 0.037	1.569AlaMet: 1.569 ± 0.011	2.199AlaAsn: 2.199 ± 0.014	3.838AlaPro: 3.838 ± 0.025	3.034AlaGln: 3.034 ± 0.017	3.515AlaArg: 3.515 ± 0.018	5.747AlaSer: 5.747 ± 0.026	3.451AlaThr: 3.451 ± 0.02	5.209AlaVal: 5.209 ± 0.022	0.716AlaTrp: 0.716 ± 0.008	1.596AlaTyr: 1.596 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	1.31CysAla: 1.31 ± 0.016	0.686CysCys: 0.686 ± 0.01	1.149CysAsp: 1.149 ± 0.017	1.269CysGlu: 1.269 ± 0.017	0.943CysPhe: 0.943 ± 0.01	1.597CysGly: 1.597 ± 0.016	0.685CysHis: 0.685 ± 0.011	0.99CysIle: 0.99 ± 0.013	1.118CysLys: 1.118 ± 0.012	2.319CysLeu: 2.319 ± 0.019	0.443CysMet: 0.443 ± 0.006	0.817CysAsn: 0.817 ± 0.011	1.379CysPro: 1.379 ± 0.019	1.112CysGln: 1.112 ± 0.014	1.439CysArg: 1.439 ± 0.012	2.2CysSer: 2.2 ± 0.021	1.195CysThr: 1.195 ± 0.012	1.656CysVal: 1.656 ± 0.015	0.338CysTrp: 0.338 ± 0.005	0.631CysTyr: 0.631 ± 0.008	0.001CysXaa: 0.001 ± 0.0
Asp	3.21AspAla: 3.21 ± 0.019	1.156AspCys: 1.156 ± 0.013	2.774AspAsp: 2.774 ± 0.02	3.468AspGlu: 3.468 ± 0.021	2.145AspPhe: 2.145 ± 0.013	3.865AspGly: 3.865 ± 0.028	1.182AspHis: 1.182 ± 0.01	2.563AspIle: 2.563 ± 0.019	2.513AspLys: 2.513 ± 0.017	5.029AspLeu: 5.029 ± 0.025	1.216AspMet: 1.216 ± 0.01	1.842AspAsn: 1.842 ± 0.015	2.897AspPro: 2.897 ± 0.017	1.96AspGln: 1.96 ± 0.013	2.741AspArg: 2.741 ± 0.019	4.157AspSer: 4.157 ± 0.024	2.474AspThr: 2.474 ± 0.015	3.393AspVal: 3.393 ± 0.019	0.704AspTrp: 0.704 ± 0.009	1.535AspTyr: 1.535 ± 0.013	0.001AspXaa: 0.001 ± 0.0
Glu	4.9GluAla: 4.9 ± 0.03	1.263GluCys: 1.263 ± 0.021	4.216GluAsp: 4.216 ± 0.023	6.809GluGlu: 6.809 ± 0.052	1.968GluPhe: 1.968 ± 0.013	4.033GluGly: 4.033 ± 0.023	1.449GluHis: 1.449 ± 0.011	2.692GluIle: 2.692 ± 0.015	4.304GluLys: 4.304 ± 0.027	6.183GluLeu: 6.183 ± 0.035	1.668GluMet: 1.668 ± 0.013	2.587GluAsn: 2.587 ± 0.017	2.788GluPro: 2.788 ± 0.019	3.047GluGln: 3.047 ± 0.022	4.196GluArg: 4.196 ± 0.025	4.049GluSer: 4.049 ± 0.022	3.174GluThr: 3.174 ± 0.018	4.201GluVal: 4.201 ± 0.021	0.68GluTrp: 0.68 ± 0.008	1.556GluTyr: 1.556 ± 0.014	0.002GluXaa: 0.002 ± 0.0
Phe	2.074PheAla: 2.074 ± 0.013	1.004PheCys: 1.004 ± 0.009	1.78PheAsp: 1.78 ± 0.014	1.903PheGlu: 1.903 ± 0.015	1.751PhePhe: 1.751 ± 0.016	2.323PheGly: 2.323 ± 0.017	1.071PheHis: 1.071 ± 0.01	1.941PheIle: 1.941 ± 0.018	1.762PheLys: 1.762 ± 0.014	4.106PheLeu: 4.106 ± 0.023	0.789PheMet: 0.789 ± 0.008	1.458PheAsn: 1.458 ± 0.012	1.912PhePro: 1.912 ± 0.014	1.721PheGln: 1.721 ± 0.011	2.052PheArg: 2.052 ± 0.017	3.517PheSer: 3.517 ± 0.022	2.294PheThr: 2.294 ± 0.016	2.387PheVal: 2.387 ± 0.016	0.507PheTrp: 0.507 ± 0.007	1.27PheTyr: 1.27 ± 0.012	0.001PheXaa: 0.001 ± 0.0
Gly	4.617GlyAla: 4.617 ± 0.033	1.376GlyCys: 1.376 ± 0.012	3.33GlyAsp: 3.33 ± 0.022	4.181GlyGlu: 4.181 ± 0.033	2.585GlyPhe: 2.585 ± 0.02	5.302GlyGly: 5.302 ± 0.033	1.709GlyHis: 1.709 ± 0.012	2.603GlyIle: 2.603 ± 0.017	3.468GlyLys: 3.468 ± 0.021	5.846GlyLeu: 5.846 ± 0.03	1.42GlyMet: 1.42 ± 0.014	2.423GlyAsn: 2.423 ± 0.017	3.944GlyPro: 3.944 ± 0.072	2.868GlyGln: 2.868 ± 0.019	3.978GlyArg: 3.978 ± 0.026	5.831GlySer: 5.831 ± 0.032	3.406GlyThr: 3.406 ± 0.022	4.17GlyVal: 4.17 ± 0.024	0.844GlyTrp: 0.844 ± 0.011	1.906GlyTyr: 1.906 ± 0.018	0.001GlyXaa: 0.001 ± 0.0
His	1.475HisAla: 1.475 ± 0.011	0.753HisCys: 0.753 ± 0.011	0.949HisAsp: 0.949 ± 0.008	1.147HisGlu: 1.147 ± 0.011	1.09HisPhe: 1.09 ± 0.009	1.612HisGly: 1.612 ± 0.014	0.96HisHis: 0.96 ± 0.011	1.261HisIle: 1.261 ± 0.01	1.237HisLys: 1.237 ± 0.011	2.875HisLeu: 2.875 ± 0.016	0.631HisMet: 0.631 ± 0.007	0.982HisAsn: 0.982 ± 0.01	1.623HisPro: 1.623 ± 0.015	1.303HisGln: 1.303 ± 0.012	1.737HisArg: 1.737 ± 0.013	2.327HisSer: 2.327 ± 0.017	1.507HisThr: 1.507 ± 0.012	1.53HisVal: 1.53 ± 0.012	0.352HisTrp: 0.352 ± 0.005	0.831HisTyr: 0.831 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	2.445IleAla: 2.445 ± 0.017	1.062IleCys: 1.062 ± 0.012	2.015IleAsp: 2.015 ± 0.015	2.192IleGlu: 2.192 ± 0.016	1.867IlePhe: 1.867 ± 0.018	2.326IleGly: 2.326 ± 0.015	1.219IleHis: 1.219 ± 0.01	2.321IleIle: 2.321 ± 0.017	2.394IleLys: 2.394 ± 0.017	4.27IleLeu: 4.27 ± 0.025	1.002IleMet: 1.002 ± 0.01	1.88IleAsn: 1.88 ± 0.012	2.369IlePro: 2.369 ± 0.017	2.155IleGln: 2.155 ± 0.014	2.504IleArg: 2.504 ± 0.016	3.658IleSer: 3.658 ± 0.02	2.621IleThr: 2.621 ± 0.018	2.553IleVal: 2.553 ± 0.017	0.487IleTrp: 0.487 ± 0.008	1.378IleTyr: 1.378 ± 0.011	0.001IleXaa: 0.001 ± 0.0
Lys	3.718LysAla: 3.718 ± 0.021	1.026LysCys: 1.026 ± 0.012	3.017LysAsp: 3.017 ± 0.017	4.301LysGlu: 4.301 ± 0.024	1.572LysPhe: 1.572 ± 0.014	3.206LysGly: 3.206 ± 0.031	1.387LysHis: 1.387 ± 0.011	2.401LysIle: 2.401 ± 0.017	4.12LysLys: 4.12 ± 0.029	4.866LysLeu: 4.866 ± 0.026	1.435LysMet: 1.435 ± 0.012	2.185LysAsn: 2.185 ± 0.014	2.691LysPro: 2.691 ± 0.02	2.394LysGln: 2.394 ± 0.017	3.304LysArg: 3.304 ± 0.018	3.589LysSer: 3.589 ± 0.021	2.928LysThr: 2.928 ± 0.018	3.5LysVal: 3.5 ± 0.021	0.559LysTrp: 0.559 ± 0.008	1.457LysTyr: 1.457 ± 0.011	0.002LysXaa: 0.002 ± 0.0
Leu	6.57LeuAla: 6.57 ± 0.029	2.353LeuCys: 2.353 ± 0.017	4.979LeuAsp: 4.979 ± 0.023	6.543LeuGlu: 6.543 ± 0.039	3.595LeuPhe: 3.595 ± 0.024	5.474LeuGly: 5.474 ± 0.025	2.83LeuHis: 2.83 ± 0.018	3.828LeuIle: 3.828 ± 0.021	5.542LeuLys: 5.542 ± 0.026	10.847LeuLeu: 10.847 ± 0.048	2.134LeuMet: 2.134 ± 0.013	3.554LeuAsn: 3.554 ± 0.019	5.456LeuPro: 5.456 ± 0.031	5.628LeuGln: 5.628 ± 0.037	6.044LeuArg: 6.044 ± 0.028	8.426LeuSer: 8.426 ± 0.039	5.204LeuThr: 5.204 ± 0.021	5.895LeuVal: 5.895 ± 0.031	1.153LeuTrp: 1.153 ± 0.011	2.685LeuTyr: 2.685 ± 0.018	0.003LeuXaa: 0.003 ± 0.0
Met	1.892MetAla: 1.892 ± 0.014	0.467MetCys: 0.467 ± 0.006	1.359MetAsp: 1.359 ± 0.011	1.89MetGlu: 1.89 ± 0.013	0.861MetPhe: 0.861 ± 0.01	1.382MetGly: 1.382 ± 0.013	0.466MetHis: 0.466 ± 0.007	0.843MetIle: 0.843 ± 0.009	1.403MetLys: 1.403 ± 0.012	2.08MetLeu: 2.08 ± 0.017	0.646MetMet: 0.646 ± 0.008	0.841MetAsn: 0.841 ± 0.008	0.992MetPro: 0.992 ± 0.011	0.929MetGln: 0.929 ± 0.011	1.164MetArg: 1.164 ± 0.011	1.782MetSer: 1.782 ± 0.012	1.194MetThr: 1.194 ± 0.012	1.568MetVal: 1.568 ± 0.011	0.258MetTrp: 0.258 ± 0.005	0.615MetTyr: 0.615 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.193AsnAla: 2.193 ± 0.015	0.867AsnCys: 0.867 ± 0.011	1.592AsnAsp: 1.592 ± 0.013	1.899AsnGlu: 1.899 ± 0.016	1.4AsnPhe: 1.4 ± 0.013	2.673AsnGly: 2.673 ± 0.022	0.97AsnHis: 0.97 ± 0.009	2.039AsnIle: 2.039 ± 0.015	2.072AsnLys: 2.072 ± 0.014	3.636AsnLeu: 3.636 ± 0.02	0.979AsnMet: 0.979 ± 0.008	1.598AsnAsn: 1.598 ± 0.014	2.19AsnPro: 2.19 ± 0.014	1.685AsnGln: 1.685 ± 0.013	2.012AsnArg: 2.012 ± 0.014	2.977AsnSer: 2.977 ± 0.017	2.09AsnThr: 2.09 ± 0.013	2.304AsnVal: 2.304 ± 0.016	0.45AsnTrp: 0.45 ± 0.007	1.137AsnTyr: 1.137 ± 0.011	0.001AsnXaa: 0.001 ± 0.0
Pro	4.539ProAla: 4.539 ± 0.028	1.113ProCys: 1.113 ± 0.014	2.88ProAsp: 2.88 ± 0.017	3.745ProGlu: 3.745 ± 0.024	1.831ProPhe: 1.831 ± 0.014	4.895ProGly: 4.895 ± 0.082	1.467ProHis: 1.467 ± 0.013	1.742ProIle: 1.742 ± 0.015	2.419ProLys: 2.419 ± 0.022	4.982ProLeu: 4.982 ± 0.025	0.95ProMet: 0.95 ± 0.011	1.836ProAsn: 1.836 ± 0.013	5.46ProPro: 5.46 ± 0.058	2.593ProGln: 2.593 ± 0.021	2.947ProArg: 2.947 ± 0.02	5.383ProSer: 5.383 ± 0.034	2.906ProThr: 2.906 ± 0.021	3.736ProVal: 3.736 ± 0.02	0.589ProTrp: 0.589 ± 0.007	1.392ProTyr: 1.392 ± 0.012	0.002ProXaa: 0.002 ± 0.0
Gln	3.328GlnAla: 3.328 ± 0.026	0.971GlnCys: 0.971 ± 0.012	2.269GlnAsp: 2.269 ± 0.015	3.416GlnGlu: 3.416 ± 0.025	1.404GlnPhe: 1.404 ± 0.012	2.831GlnGly: 2.831 ± 0.021	1.349GlnHis: 1.349 ± 0.013	1.901GlnIle: 1.901 ± 0.013	2.568GlnLys: 2.568 ± 0.014	4.681GlnLeu: 4.681 ± 0.029	1.123GlnMet: 1.123 ± 0.012	1.739GlnAsn: 1.739 ± 0.013	2.559GlnPro: 2.559 ± 0.02	3.026GlnGln: 3.026 ± 0.03	3.203GlnArg: 3.203 ± 0.02	3.289GlnSer: 3.289 ± 0.019	2.459GlnThr: 2.459 ± 0.016	2.907GlnVal: 2.907 ± 0.018	0.555GlnTrp: 0.555 ± 0.007	1.215GlnTyr: 1.215 ± 0.01	0.002GlnXaa: 0.002 ± 0.0
Arg	3.802ArgAla: 3.802 ± 0.02	1.462ArgCys: 1.462 ± 0.018	2.954ArgAsp: 2.954 ± 0.019	3.805ArgGlu: 3.805 ± 0.025	2.118ArgPhe: 2.118 ± 0.014	3.798ArgGly: 3.798 ± 0.033	1.602ArgHis: 1.602 ± 0.013	2.425ArgIle: 2.425 ± 0.015	3.477ArgLys: 3.477 ± 0.019	5.729ArgLeu: 5.729 ± 0.029	1.309ArgMet: 1.309 ± 0.01	2.138ArgAsn: 2.138 ± 0.014	3.129ArgPro: 3.129 ± 0.022	2.739ArgGln: 2.739 ± 0.019	4.746ArgArg: 4.746 ± 0.032	4.799ArgSer: 4.799 ± 0.031	3.006ArgThr: 3.006 ± 0.018	3.54ArgVal: 3.54 ± 0.021	0.778ArgTrp: 0.778 ± 0.008	1.659ArgTyr: 1.659 ± 0.012	0.001ArgXaa: 0.001 ± 0.0
Ser	5.882SerAla: 5.882 ± 0.027	2.045SerCys: 2.045 ± 0.02	4.123SerAsp: 4.123 ± 0.021	4.69SerGlu: 4.69 ± 0.022	3.173SerPhe: 3.173 ± 0.018	5.732SerGly: 5.732 ± 0.028	2.172SerHis: 2.172 ± 0.016	3.197SerIle: 3.197 ± 0.018	3.785SerLys: 3.785 ± 0.02	8.318SerLeu: 8.318 ± 0.032	1.677SerMet: 1.677 ± 0.012	2.79SerAsn: 2.79 ± 0.016	5.663SerPro: 5.663 ± 0.039	3.774SerGln: 3.774 ± 0.02	4.794SerArg: 4.794 ± 0.031	9.517SerSer: 9.517 ± 0.056	4.45SerThr: 4.45 ± 0.025	5.498SerVal: 5.498 ± 0.023	1.061SerTrp: 1.061 ± 0.012	2.141SerTyr: 2.141 ± 0.013	0.003SerXaa: 0.003 ± 0.0
Thr	3.972ThrAla: 3.972 ± 0.02	1.415ThrCys: 1.415 ± 0.02	2.689ThrAsp: 2.689 ± 0.019	3.373ThrGlu: 3.373 ± 0.019	2.176ThrPhe: 2.176 ± 0.016	3.681ThrGly: 3.681 ± 0.026	1.333ThrHis: 1.333 ± 0.012	2.328ThrIle: 2.328 ± 0.015	2.475ThrLys: 2.475 ± 0.018	5.391ThrLeu: 5.391 ± 0.021	1.134ThrMet: 1.134 ± 0.01	1.769ThrAsn: 1.769 ± 0.012	3.358ThrPro: 3.358 ± 0.022	2.221ThrGln: 2.221 ± 0.016	2.574ThrArg: 2.574 ± 0.015	4.529ThrSer: 4.529 ± 0.022	2.927ThrThr: 2.927 ± 0.021	4.127ThrVal: 4.127 ± 0.025	0.682ThrTrp: 0.682 ± 0.009	1.44ThrTyr: 1.44 ± 0.012	0.003ThrXaa: 0.003 ± 0.0
Val	4.467ValAla: 4.467 ± 0.022	1.825ValCys: 1.825 ± 0.016	3.254ValAsp: 3.254 ± 0.022	4.012ValGlu: 4.012 ± 0.025	2.833ValPhe: 2.833 ± 0.016	3.761ValGly: 3.761 ± 0.022	1.647ValHis: 1.647 ± 0.013	2.938ValIle: 2.938 ± 0.019	3.465ValLys: 3.465 ± 0.021	6.661ValLeu: 6.661 ± 0.032	1.506ValMet: 1.506 ± 0.012	2.416ValAsn: 2.416 ± 0.015	3.395ValPro: 3.395 ± 0.019	2.835ValGln: 2.835 ± 0.018	3.515ValArg: 3.515 ± 0.018	5.39ValSer: 5.39 ± 0.023	3.95ValThr: 3.95 ± 0.025	4.611ValVal: 4.611 ± 0.025	0.831ValTrp: 0.831 ± 0.009	1.886ValTyr: 1.886 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.742TrpAla: 0.742 ± 0.007	0.266TrpCys: 0.266 ± 0.005	0.66TrpAsp: 0.66 ± 0.009	0.764TrpGlu: 0.764 ± 0.01	0.509TrpPhe: 0.509 ± 0.007	0.657TrpGly: 0.657 ± 0.009	0.291TrpHis: 0.291 ± 0.005	0.556TrpIle: 0.556 ± 0.008	0.739TrpLys: 0.739 ± 0.009	1.249TrpLeu: 1.249 ± 0.013	0.352TrpMet: 0.352 ± 0.006	0.528TrpAsn: 0.528 ± 0.007	0.472TrpPro: 0.472 ± 0.007	0.498TrpGln: 0.498 ± 0.007	0.809TrpArg: 0.809 ± 0.008	1.009TrpSer: 1.009 ± 0.011	0.746TrpThr: 0.746 ± 0.009	0.747TrpVal: 0.747 ± 0.008	0.216TrpTrp: 0.216 ± 0.005	0.351TrpTyr: 0.351 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.519TyrAla: 1.519 ± 0.012	0.731TyrCys: 0.731 ± 0.009	1.397TyrAsp: 1.397 ± 0.013	1.564TyrGlu: 1.564 ± 0.012	1.236TyrPhe: 1.236 ± 0.012	1.718TyrGly: 1.718 ± 0.014	0.797TyrHis: 0.797 ± 0.008	1.423TyrIle: 1.423 ± 0.011	1.428TyrLys: 1.428 ± 0.012	2.754TyrLeu: 2.754 ± 0.017	0.649TyrMet: 0.649 ± 0.008	1.179TyrAsn: 1.179 ± 0.011	1.289TyrPro: 1.289 ± 0.011	1.272TyrGln: 1.272 ± 0.01	1.741TyrArg: 1.741 ± 0.013	2.303TyrSer: 2.303 ± 0.015	1.612TyrThr: 1.612 ± 0.014	1.665TyrVal: 1.665 ± 0.013	0.405TyrTrp: 0.405 ± 0.008	0.995TyrTyr: 0.995 ± 0.011	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.003XaaLys: 0.003 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.007XaaXaa: 0.007 ± 0.002

Statistics based on 23074 proteins (12616183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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