Klebsiella phage Mulock

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T7I9|A0A5J6T7I9_9CAUD DNA binding protein OS=Klebsiella phage Mulock OX=2650873 GN=CPT_Mulock_051 PE=4 SV=1
MM1 pKa = 7.45TISEE5 pKa = 4.57IPLSPEE11 pKa = 3.45NQRR14 pKa = 11.84FSISVAGQSLQMAVTWRR31 pKa = 11.84AAFWCLDD38 pKa = 3.14IMDD41 pKa = 4.75SSGADD46 pKa = 4.27LIRR49 pKa = 11.84GIPLITGSDD58 pKa = 3.41LLAQYY63 pKa = 10.54RR64 pKa = 11.84YY65 pKa = 10.55LEE67 pKa = 4.71LGFSLYY73 pKa = 10.52VGCDD77 pKa = 3.3NQSSEE82 pKa = 4.45NPTEE86 pKa = 4.27ADD88 pKa = 3.19LGIYY92 pKa = 9.15SHH94 pKa = 7.09LYY96 pKa = 10.39AVTEE100 pKa = 4.11

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T9B3|A0A5J6T9B3_9CAUD Uncharacterized protein OS=Klebsiella phage Mulock OX=2650873 GN=CPT_Mulock_043 PE=4 SV=1
MM1 pKa = 7.06QCLLAKK7 pKa = 10.46VMEE10 pKa = 4.54RR11 pKa = 11.84GIFRR15 pKa = 11.84VPARR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.69RR22 pKa = 11.84KK23 pKa = 10.09VEE25 pKa = 4.06VKK27 pKa = 10.41PSDD30 pKa = 3.27IPTLKK35 pKa = 10.58DD36 pKa = 3.07YY37 pKa = 7.67TARR40 pKa = 11.84LVDD43 pKa = 4.74KK44 pKa = 10.76KK45 pKa = 9.64WLCLRR50 pKa = 11.84ARR52 pKa = 11.84RR53 pKa = 11.84PHH55 pKa = 5.84AA56 pKa = 4.31

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13795

36

1275

205.9

22.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.873 ± 0.373

1.116 ± 0.143

6.075 ± 0.214

6.082 ± 0.385

3.422 ± 0.217

7.568 ± 0.365

1.493 ± 0.119

5.618 ± 0.216

5.415 ± 0.325

7.771 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.17

4.502 ± 0.271

4.183 ± 0.213

3.893 ± 0.18

5.864 ± 0.302

6.473 ± 0.245

6.227 ± 0.389

6.691 ± 0.29

1.791 ± 0.155

3.24 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski