Amino acid dipepetide frequency for Klebsiella phage Mulock

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.309AlaAla: 11.309 ± 1.053
0.58AlaCys: 0.58 ± 0.208
7.105AlaAsp: 7.105 ± 0.78
6.38AlaGlu: 6.38 ± 1.113
2.61AlaPhe: 2.61 ± 0.42
8.047AlaGly: 8.047 ± 0.739
1.74AlaHis: 1.74 ± 0.537
6.162AlaIle: 6.162 ± 0.689
6.017AlaLys: 6.017 ± 0.659
8.119AlaLeu: 8.119 ± 0.798
3.48AlaMet: 3.48 ± 0.527
3.842AlaAsn: 3.842 ± 0.647
2.465AlaPro: 2.465 ± 0.479
4.495AlaGln: 4.495 ± 0.488
5.147AlaArg: 5.147 ± 0.678
5.582AlaSer: 5.582 ± 0.622
4.712AlaThr: 4.712 ± 0.739
6.815AlaVal: 6.815 ± 0.652
1.377AlaTrp: 1.377 ± 0.277
2.682AlaTyr: 2.682 ± 0.468
0.0AlaXaa: 0.0 ± 0.0
Cys
0.87CysAla: 0.87 ± 0.279
0.435CysCys: 0.435 ± 0.198
0.507CysAsp: 0.507 ± 0.185
0.29CysGlu: 0.29 ± 0.142
0.29CysPhe: 0.29 ± 0.14
1.015CysGly: 1.015 ± 0.262
0.217CysHis: 0.217 ± 0.112
0.58CysIle: 0.58 ± 0.204
0.797CysLys: 0.797 ± 0.205
1.45CysLeu: 1.45 ± 0.354
0.217CysMet: 0.217 ± 0.125
0.217CysAsn: 0.217 ± 0.149
0.87CysPro: 0.87 ± 0.342
0.217CysGln: 0.217 ± 0.125
0.725CysArg: 0.725 ± 0.249
0.652CysSer: 0.652 ± 0.23
0.725CysThr: 0.725 ± 0.223
0.507CysVal: 0.507 ± 0.224
0.29CysTrp: 0.29 ± 0.135
0.29CysTyr: 0.29 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
5.727AspAla: 5.727 ± 0.82
0.942AspCys: 0.942 ± 0.235
3.045AspAsp: 3.045 ± 0.409
4.205AspGlu: 4.205 ± 0.52
2.827AspPhe: 2.827 ± 0.357
5.51AspGly: 5.51 ± 0.722
1.087AspHis: 1.087 ± 0.275
3.987AspIle: 3.987 ± 0.498
2.972AspLys: 2.972 ± 0.43
4.205AspLeu: 4.205 ± 0.509
1.595AspMet: 1.595 ± 0.28
2.175AspAsn: 2.175 ± 0.318
2.03AspPro: 2.03 ± 0.388
2.03AspGln: 2.03 ± 0.368
3.262AspArg: 3.262 ± 0.401
3.625AspSer: 3.625 ± 0.462
3.045AspThr: 3.045 ± 0.541
5.22AspVal: 5.22 ± 0.735
1.522AspTrp: 1.522 ± 0.378
1.74AspTyr: 1.74 ± 0.281
0.0AspXaa: 0.0 ± 0.0
Glu
6.525GluAla: 6.525 ± 0.868
0.87GluCys: 0.87 ± 0.257
2.9GluAsp: 2.9 ± 0.501
3.335GluGlu: 3.335 ± 0.755
2.9GluPhe: 2.9 ± 0.532
4.132GluGly: 4.132 ± 0.686
0.507GluHis: 0.507 ± 0.141
3.77GluIle: 3.77 ± 0.547
3.262GluLys: 3.262 ± 0.649
5.22GluLeu: 5.22 ± 0.599
2.32GluMet: 2.32 ± 0.32
2.827GluAsn: 2.827 ± 0.539
2.392GluPro: 2.392 ± 0.359
3.117GluGln: 3.117 ± 0.645
4.06GluArg: 4.06 ± 0.742
3.915GluSer: 3.915 ± 0.571
2.755GluThr: 2.755 ± 0.483
2.827GluVal: 2.827 ± 0.504
1.45GluTrp: 1.45 ± 0.321
1.74GluTyr: 1.74 ± 0.357
0.0GluXaa: 0.0 ± 0.0
Phe
2.755PheAla: 2.755 ± 0.416
0.29PheCys: 0.29 ± 0.137
2.972PheAsp: 2.972 ± 0.49
1.74PheGlu: 1.74 ± 0.402
1.377PhePhe: 1.377 ± 0.32
2.03PheGly: 2.03 ± 0.35
0.29PheHis: 0.29 ± 0.174
1.595PheIle: 1.595 ± 0.283
1.885PheLys: 1.885 ± 0.371
2.392PheLeu: 2.392 ± 0.496
0.652PheMet: 0.652 ± 0.258
2.537PheAsn: 2.537 ± 0.498
1.667PhePro: 1.667 ± 0.321
0.942PheGln: 0.942 ± 0.269
2.465PheArg: 2.465 ± 0.375
2.32PheSer: 2.32 ± 0.423
2.32PheThr: 2.32 ± 0.386
2.175PheVal: 2.175 ± 0.425
0.725PheTrp: 0.725 ± 0.217
1.087PheTyr: 1.087 ± 0.375
0.0PheXaa: 0.0 ± 0.0
Gly
6.742GlyAla: 6.742 ± 0.706
1.015GlyCys: 1.015 ± 0.272
5.147GlyAsp: 5.147 ± 0.661
3.915GlyGlu: 3.915 ± 0.613
2.972GlyPhe: 2.972 ± 0.412
5.002GlyGly: 5.002 ± 1.071
0.797GlyHis: 0.797 ± 0.231
4.93GlyIle: 4.93 ± 0.522
4.567GlyLys: 4.567 ± 0.547
6.09GlyLeu: 6.09 ± 0.624
2.537GlyMet: 2.537 ± 0.437
4.132GlyAsn: 4.132 ± 0.814
2.102GlyPro: 2.102 ± 0.361
2.682GlyGln: 2.682 ± 0.429
3.552GlyArg: 3.552 ± 0.563
4.132GlySer: 4.132 ± 0.615
5.22GlyThr: 5.22 ± 0.795
6.017GlyVal: 6.017 ± 0.766
1.232GlyTrp: 1.232 ± 0.305
2.9GlyTyr: 2.9 ± 0.479
0.0GlyXaa: 0.0 ± 0.0
His
1.232HisAla: 1.232 ± 0.278
0.362HisCys: 0.362 ± 0.167
0.87HisAsp: 0.87 ± 0.223
0.942HisGlu: 0.942 ± 0.365
0.58HisPhe: 0.58 ± 0.223
1.812HisGly: 1.812 ± 0.282
0.507HisHis: 0.507 ± 0.205
0.507HisIle: 0.507 ± 0.17
0.797HisLys: 0.797 ± 0.226
0.942HisLeu: 0.942 ± 0.274
0.072HisMet: 0.072 ± 0.071
0.507HisAsn: 0.507 ± 0.219
0.435HisPro: 0.435 ± 0.18
0.362HisGln: 0.362 ± 0.145
1.377HisArg: 1.377 ± 0.31
0.797HisSer: 0.797 ± 0.227
1.015HisThr: 1.015 ± 0.296
1.015HisVal: 1.015 ± 0.259
0.217HisTrp: 0.217 ± 0.129
0.58HisTyr: 0.58 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
5.8IleAla: 5.8 ± 0.734
0.942IleCys: 0.942 ± 0.284
3.407IleAsp: 3.407 ± 0.468
4.205IleGlu: 4.205 ± 0.569
1.667IlePhe: 1.667 ± 0.252
4.422IleGly: 4.422 ± 0.614
0.652IleHis: 0.652 ± 0.211
2.827IleIle: 2.827 ± 0.399
3.045IleLys: 3.045 ± 0.507
3.335IleLeu: 3.335 ± 0.556
1.16IleMet: 1.16 ± 0.276
2.392IleAsn: 2.392 ± 0.555
2.682IlePro: 2.682 ± 0.516
2.32IleGln: 2.32 ± 0.399
3.48IleArg: 3.48 ± 0.419
4.132IleSer: 4.132 ± 0.497
4.205IleThr: 4.205 ± 0.652
2.682IleVal: 2.682 ± 0.434
0.58IleTrp: 0.58 ± 0.173
2.247IleTyr: 2.247 ± 0.386
0.0IleXaa: 0.0 ± 0.0
Lys
6.307LysAla: 6.307 ± 0.773
0.362LysCys: 0.362 ± 0.153
3.335LysAsp: 3.335 ± 0.463
2.755LysGlu: 2.755 ± 0.505
2.03LysPhe: 2.03 ± 0.385
2.392LysGly: 2.392 ± 0.525
1.015LysHis: 1.015 ± 0.263
3.117LysIle: 3.117 ± 0.548
3.262LysLys: 3.262 ± 0.652
3.697LysLeu: 3.697 ± 0.518
1.45LysMet: 1.45 ± 0.378
2.247LysAsn: 2.247 ± 0.338
2.392LysPro: 2.392 ± 0.442
2.537LysGln: 2.537 ± 0.404
4.06LysArg: 4.06 ± 0.582
3.842LysSer: 3.842 ± 0.465
3.407LysThr: 3.407 ± 0.562
3.045LysVal: 3.045 ± 0.53
1.232LysTrp: 1.232 ± 0.314
1.667LysTyr: 1.667 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
7.829LeuAla: 7.829 ± 0.945
0.87LeuCys: 0.87 ± 0.269
4.205LeuAsp: 4.205 ± 0.618
4.35LeuGlu: 4.35 ± 0.522
2.03LeuPhe: 2.03 ± 0.423
5.655LeuGly: 5.655 ± 0.499
1.232LeuHis: 1.232 ± 0.289
4.785LeuIle: 4.785 ± 0.665
5.147LeuLys: 5.147 ± 0.682
6.235LeuLeu: 6.235 ± 0.547
2.392LeuMet: 2.392 ± 0.436
3.335LeuAsn: 3.335 ± 0.572
3.842LeuPro: 3.842 ± 0.663
2.537LeuGln: 2.537 ± 0.397
4.132LeuArg: 4.132 ± 0.641
5.51LeuSer: 5.51 ± 0.628
5.22LeuThr: 5.22 ± 0.627
4.785LeuVal: 4.785 ± 0.715
1.16LeuTrp: 1.16 ± 0.283
2.465LeuTyr: 2.465 ± 0.39
0.0LeuXaa: 0.0 ± 0.0
Met
3.19MetAla: 3.19 ± 0.557
0.072MetCys: 0.072 ± 0.072
1.595MetAsp: 1.595 ± 0.315
1.377MetGlu: 1.377 ± 0.242
0.797MetPhe: 0.797 ± 0.215
0.942MetGly: 0.942 ± 0.283
0.435MetHis: 0.435 ± 0.189
1.377MetIle: 1.377 ± 0.366
1.74MetLys: 1.74 ± 0.425
2.102MetLeu: 2.102 ± 0.461
0.58MetMet: 0.58 ± 0.202
1.16MetAsn: 1.16 ± 0.282
1.522MetPro: 1.522 ± 0.345
1.16MetGln: 1.16 ± 0.23
1.667MetArg: 1.667 ± 0.438
2.175MetSer: 2.175 ± 0.45
2.247MetThr: 2.247 ± 0.365
1.595MetVal: 1.595 ± 0.376
0.435MetTrp: 0.435 ± 0.19
0.87MetTyr: 0.87 ± 0.245
0.0MetXaa: 0.0 ± 0.0
Asn
3.842AsnAla: 3.842 ± 0.641
0.217AsnCys: 0.217 ± 0.11
1.885AsnAsp: 1.885 ± 0.342
2.537AsnGlu: 2.537 ± 0.444
2.102AsnPhe: 2.102 ± 0.417
4.712AsnGly: 4.712 ± 0.708
0.652AsnHis: 0.652 ± 0.201
1.812AsnIle: 1.812 ± 0.451
2.175AsnLys: 2.175 ± 0.413
2.827AsnLeu: 2.827 ± 0.415
0.942AsnMet: 0.942 ± 0.223
2.61AsnAsn: 2.61 ± 0.508
2.537AsnPro: 2.537 ± 0.336
2.247AsnGln: 2.247 ± 0.422
1.885AsnArg: 1.885 ± 0.321
3.117AsnSer: 3.117 ± 0.522
3.407AsnThr: 3.407 ± 0.497
3.045AsnVal: 3.045 ± 0.521
0.725AsnTrp: 0.725 ± 0.199
1.74AsnTyr: 1.74 ± 0.321
0.0AsnXaa: 0.0 ± 0.0
Pro
4.277ProAla: 4.277 ± 0.607
0.362ProCys: 0.362 ± 0.174
3.262ProAsp: 3.262 ± 0.487
4.277ProGlu: 4.277 ± 0.611
1.45ProPhe: 1.45 ± 0.309
3.262ProGly: 3.262 ± 0.633
0.435ProHis: 0.435 ± 0.151
1.45ProIle: 1.45 ± 0.276
2.247ProLys: 2.247 ± 0.389
3.045ProLeu: 3.045 ± 0.591
0.797ProMet: 0.797 ± 0.24
1.885ProAsn: 1.885 ± 0.285
1.595ProPro: 1.595 ± 0.423
1.305ProGln: 1.305 ± 0.332
1.45ProArg: 1.45 ± 0.295
2.175ProSer: 2.175 ± 0.376
2.392ProThr: 2.392 ± 0.36
4.277ProVal: 4.277 ± 0.65
0.435ProTrp: 0.435 ± 0.173
1.45ProTyr: 1.45 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
5.075GlnAla: 5.075 ± 0.59
0.725GlnCys: 0.725 ± 0.214
1.305GlnAsp: 1.305 ± 0.337
1.667GlnGlu: 1.667 ± 0.299
1.595GlnPhe: 1.595 ± 0.312
1.812GlnGly: 1.812 ± 0.366
0.725GlnHis: 0.725 ± 0.221
2.102GlnIle: 2.102 ± 0.356
2.247GlnLys: 2.247 ± 0.488
3.915GlnLeu: 3.915 ± 0.461
1.087GlnMet: 1.087 ± 0.261
1.74GlnAsn: 1.74 ± 0.331
1.595GlnPro: 1.595 ± 0.358
1.885GlnGln: 1.885 ± 0.341
1.74GlnArg: 1.74 ± 0.447
2.9GlnSer: 2.9 ± 0.553
2.03GlnThr: 2.03 ± 0.396
2.247GlnVal: 2.247 ± 0.349
0.942GlnTrp: 0.942 ± 0.243
1.595GlnTyr: 1.595 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
4.422ArgAla: 4.422 ± 0.514
0.435ArgCys: 0.435 ± 0.161
3.77ArgAsp: 3.77 ± 0.532
4.712ArgGlu: 4.712 ± 0.995
1.667ArgPhe: 1.667 ± 0.392
3.335ArgGly: 3.335 ± 0.571
1.377ArgHis: 1.377 ± 0.341
3.407ArgIle: 3.407 ± 0.635
3.987ArgLys: 3.987 ± 0.521
5.22ArgLeu: 5.22 ± 0.662
1.957ArgMet: 1.957 ± 0.418
2.32ArgAsn: 2.32 ± 0.428
2.32ArgPro: 2.32 ± 0.392
2.755ArgGln: 2.755 ± 0.432
4.422ArgArg: 4.422 ± 0.699
3.552ArgSer: 3.552 ± 0.453
2.537ArgThr: 2.537 ± 0.374
3.335ArgVal: 3.335 ± 0.509
1.305ArgTrp: 1.305 ± 0.343
1.812ArgTyr: 1.812 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
5.945SerAla: 5.945 ± 0.578
0.507SerCys: 0.507 ± 0.212
4.277SerAsp: 4.277 ± 0.649
4.277SerGlu: 4.277 ± 0.627
2.03SerPhe: 2.03 ± 0.413
5.727SerGly: 5.727 ± 0.684
0.87SerHis: 0.87 ± 0.251
3.117SerIle: 3.117 ± 0.346
3.117SerLys: 3.117 ± 0.467
5.945SerLeu: 5.945 ± 0.698
1.885SerMet: 1.885 ± 0.368
2.682SerAsn: 2.682 ± 0.476
3.045SerPro: 3.045 ± 0.393
2.03SerGln: 2.03 ± 0.398
3.987SerArg: 3.987 ± 0.514
4.35SerSer: 4.35 ± 0.678
3.48SerThr: 3.48 ± 0.486
5.22SerVal: 5.22 ± 0.747
0.942SerTrp: 0.942 ± 0.268
1.305SerTyr: 1.305 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
6.525ThrAla: 6.525 ± 0.661
0.507ThrCys: 0.507 ± 0.166
3.19ThrAsp: 3.19 ± 0.47
3.335ThrGlu: 3.335 ± 0.522
1.812ThrPhe: 1.812 ± 0.354
6.597ThrGly: 6.597 ± 0.895
0.87ThrHis: 0.87 ± 0.296
4.132ThrIle: 4.132 ± 0.657
1.595ThrLys: 1.595 ± 0.34
5.365ThrLeu: 5.365 ± 0.615
0.942ThrMet: 0.942 ± 0.247
2.392ThrAsn: 2.392 ± 0.484
3.552ThrPro: 3.552 ± 0.481
2.03ThrGln: 2.03 ± 0.491
2.755ThrArg: 2.755 ± 0.436
3.625ThrSer: 3.625 ± 0.558
4.422ThrThr: 4.422 ± 0.672
5.075ThrVal: 5.075 ± 0.764
1.522ThrTrp: 1.522 ± 0.341
2.03ThrTyr: 2.03 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
6.452ValAla: 6.452 ± 0.797
0.725ValCys: 0.725 ± 0.25
4.64ValAsp: 4.64 ± 0.725
3.842ValGlu: 3.842 ± 0.492
2.175ValPhe: 2.175 ± 0.359
4.93ValGly: 4.93 ± 0.577
0.725ValHis: 0.725 ± 0.189
3.625ValIle: 3.625 ± 0.503
3.48ValLys: 3.48 ± 0.514
4.35ValLeu: 4.35 ± 0.568
1.45ValMet: 1.45 ± 0.372
3.77ValAsn: 3.77 ± 0.565
3.117ValPro: 3.117 ± 0.499
2.03ValGln: 2.03 ± 0.335
4.06ValArg: 4.06 ± 0.613
4.785ValSer: 4.785 ± 0.662
5.51ValThr: 5.51 ± 0.729
4.132ValVal: 4.132 ± 0.634
0.725ValTrp: 0.725 ± 0.273
2.392ValTyr: 2.392 ± 0.504
0.0ValXaa: 0.0 ± 0.0
Trp
1.522TrpAla: 1.522 ± 0.375
0.58TrpCys: 0.58 ± 0.19
1.16TrpAsp: 1.16 ± 0.3
1.305TrpGlu: 1.305 ± 0.269
0.652TrpPhe: 0.652 ± 0.176
1.305TrpGly: 1.305 ± 0.287
0.29TrpHis: 0.29 ± 0.123
1.087TrpIle: 1.087 ± 0.259
0.652TrpLys: 0.652 ± 0.23
1.45TrpLeu: 1.45 ± 0.286
0.725TrpMet: 0.725 ± 0.249
0.797TrpAsn: 0.797 ± 0.224
0.362TrpPro: 0.362 ± 0.16
0.652TrpGln: 0.652 ± 0.241
1.16TrpArg: 1.16 ± 0.321
1.595TrpSer: 1.595 ± 0.321
0.87TrpThr: 0.87 ± 0.252
0.797TrpVal: 0.797 ± 0.243
0.435TrpTrp: 0.435 ± 0.205
0.507TrpTyr: 0.507 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.392TyrAla: 2.392 ± 0.488
0.362TyrCys: 0.362 ± 0.154
2.175TyrAsp: 2.175 ± 0.35
1.377TyrGlu: 1.377 ± 0.326
0.652TyrPhe: 0.652 ± 0.213
3.045TyrGly: 3.045 ± 0.381
0.507TyrHis: 0.507 ± 0.177
1.812TyrIle: 1.812 ± 0.441
1.16TyrLys: 1.16 ± 0.283
1.812TyrLeu: 1.812 ± 0.411
0.725TyrMet: 0.725 ± 0.218
1.45TyrAsn: 1.45 ± 0.512
1.305TyrPro: 1.305 ± 0.22
1.595TyrGln: 1.595 ± 0.353
3.262TyrArg: 3.262 ± 0.405
1.957TyrSer: 1.957 ± 0.335
2.755TyrThr: 2.755 ± 0.512
2.102TyrVal: 2.102 ± 0.384
0.652TyrTrp: 0.652 ± 0.19
1.305TyrTyr: 1.305 ± 0.267
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (13795 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski