Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Ignisphaera; Ignisphaera aggregans

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1929 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0SS57|E0SS57_IGNAA Tryptophan synthase OS=Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1) OX=583356 GN=Igag_1717 PE=3 SV=1
MM1 pKa = 7.26NRR3 pKa = 11.84RR4 pKa = 11.84LISFSLILLTLLTVATLAMWSEE26 pKa = 4.17TLRR29 pKa = 11.84VNVAVGTGEE38 pKa = 3.99VDD40 pKa = 3.1WEE42 pKa = 4.43IVSGPTTWLDD52 pKa = 3.31ACGLQPGYY60 pKa = 11.17GYY62 pKa = 10.83FRR64 pKa = 11.84GNDD67 pKa = 3.08WNASYY72 pKa = 10.71LPEE75 pKa = 5.18PGADD79 pKa = 3.44QLDD82 pKa = 4.07KK83 pKa = 11.45DD84 pKa = 4.36VGCTDD89 pKa = 3.02VTLIDD94 pKa = 3.7SDD96 pKa = 4.2GDD98 pKa = 3.47GDD100 pKa = 4.6YY101 pKa = 10.7DD102 pKa = 3.82TMRR105 pKa = 11.84VTLYY109 pKa = 10.77NVYY112 pKa = 9.24PWYY115 pKa = 8.59YY116 pKa = 7.8THH118 pKa = 7.88IAFKK122 pKa = 10.28VHH124 pKa = 6.79NDD126 pKa = 2.86GTIPIKK132 pKa = 9.59IWRR135 pKa = 11.84IIIDD139 pKa = 3.36NQEE142 pKa = 4.02FYY144 pKa = 11.17EE145 pKa = 5.34LNAQPLKK152 pKa = 10.77EE153 pKa = 4.08GLYY156 pKa = 10.7LDD158 pKa = 3.86LTGDD162 pKa = 3.76GKK164 pKa = 11.41SDD166 pKa = 3.46VLIWWGDD173 pKa = 3.38NFGVQLHH180 pKa = 6.9PCQSADD186 pKa = 2.93ISLDD190 pKa = 3.11ITVLQDD196 pKa = 3.27APQGSTLSFTVSFEE210 pKa = 4.32AIQWNEE216 pKa = 3.82YY217 pKa = 9.36YY218 pKa = 10.86VPSS221 pKa = 4.03

Molecular weight: 24.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|E0SQX5|RRP4_IGNAA Exosome complex component Rrp4 OS=Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1) OX=583356 GN=rrp4 PE=3 SV=1
MM1 pKa = 7.72VSLLRR6 pKa = 11.84CNDD9 pKa = 2.81NVIRR13 pKa = 11.84VGKK16 pKa = 9.13KK17 pKa = 8.41FYY19 pKa = 9.64MRR21 pKa = 11.84YY22 pKa = 8.73VAAILYY28 pKa = 8.96KK29 pKa = 10.58RR30 pKa = 11.84NIEE33 pKa = 4.03KK34 pKa = 10.55CSTIMLRR41 pKa = 11.84ALGRR45 pKa = 11.84NISRR49 pKa = 11.84AISIATAAMFLDD61 pKa = 3.57SSLYY65 pKa = 9.68PSEE68 pKa = 4.68ARR70 pKa = 11.84IFSIFLSSGGRR81 pKa = 11.84EE82 pKa = 3.9LRR84 pKa = 11.84KK85 pKa = 10.27SGIEE89 pKa = 3.54LVLRR93 pKa = 11.84VRR95 pKa = 11.84DD96 pKa = 5.11FIPP99 pKa = 3.84

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1929

0

1929

537386

30

1967

278.6

31.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.054 ± 0.052

0.913 ± 0.018

5.195 ± 0.039

6.773 ± 0.069

3.609 ± 0.036

6.198 ± 0.052

1.469 ± 0.024

11.464 ± 0.07

5.914 ± 0.056

9.753 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.023

3.955 ± 0.038

3.968 ± 0.04

1.733 ± 0.027

6.302 ± 0.05

6.613 ± 0.049

4.542 ± 0.056

7.367 ± 0.052

1.05 ± 0.023

4.762 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski