Streptococcus phage Javan351

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A9E1|A0A4D6A9E1_9CAUD Putative head morphogenesis protein OS=Streptococcus phage Javan351 OX=2548122 GN=Javan351_0032 PE=4 SV=1
MM1 pKa = 7.82IINNYY6 pKa = 9.48KK7 pKa = 9.81YY8 pKa = 9.88DD9 pKa = 3.76YY10 pKa = 10.73SSGRR14 pKa = 11.84ICYY17 pKa = 9.2TIDD20 pKa = 2.91VDD22 pKa = 4.55GYY24 pKa = 8.79EE25 pKa = 4.41KK26 pKa = 11.18AMEE29 pKa = 4.17HH30 pKa = 6.15TKK32 pKa = 10.05TEE34 pKa = 4.15YY35 pKa = 11.24GSVQRR40 pKa = 11.84NDD42 pKa = 2.89IDD44 pKa = 5.02DD45 pKa = 4.24FLSTVEE51 pKa = 4.76EE52 pKa = 4.35YY53 pKa = 11.07DD54 pKa = 3.51FQEE57 pKa = 5.91AEE59 pKa = 4.15MIEE62 pKa = 4.27AFVDD66 pKa = 4.31FQNDD70 pKa = 3.24LLLYY74 pKa = 10.69GIGFEE79 pKa = 4.87LRR81 pKa = 11.84NEE83 pKa = 4.22VTRR86 pKa = 4.42

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A8T4|A0A4D6A8T4_9CAUD Putative DNA-directed RNA polymerase OS=Streptococcus phage Javan351 OX=2548122 GN=Javan351_0010 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.65PKK4 pKa = 10.27KK5 pKa = 10.1YY6 pKa = 9.81PYY8 pKa = 9.8SGKK11 pKa = 10.49LKK13 pKa = 10.67LIRR16 pKa = 11.84QTLPRR21 pKa = 11.84FVLLGNAAFNSNLVKK36 pKa = 10.99YY37 pKa = 10.11IDD39 pKa = 4.24TIKK42 pKa = 10.51QVAPNQTIVYY52 pKa = 7.88FKK54 pKa = 10.19IPKK57 pKa = 9.47FLSHH61 pKa = 5.9EE62 pKa = 4.25EE63 pKa = 4.11KK64 pKa = 10.76YY65 pKa = 11.07VRR67 pKa = 11.84VPLKK71 pKa = 9.7IGEE74 pKa = 4.34VVKK77 pKa = 10.77ILNRR81 pKa = 3.69

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11374

50

1058

210.6

23.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.086 ± 0.484

0.598 ± 0.137

6.172 ± 0.24

7.165 ± 0.486

4.589 ± 0.241

6.823 ± 0.581

1.407 ± 0.184

6.84 ± 0.277

8.563 ± 0.384

7.544 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.139

5.469 ± 0.304

2.875 ± 0.213

4.396 ± 0.245

4.176 ± 0.277

6.295 ± 0.323

6.005 ± 0.265

6.357 ± 0.251

1.31 ± 0.158

3.895 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski