Amino acid dipepetide frequency for Pseudarcicella hirudinis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.829AlaAla: 4.829 ± 0.067	0.642AlaCys: 0.642 ± 0.021	3.804AlaAsp: 3.804 ± 0.047	4.378AlaGlu: 4.378 ± 0.051	3.51AlaPhe: 3.51 ± 0.049	5.302AlaGly: 5.302 ± 0.072	1.034AlaHis: 1.034 ± 0.026	4.939AlaIle: 4.939 ± 0.059	4.605AlaLys: 4.605 ± 0.061	6.359AlaLeu: 6.359 ± 0.065	1.485AlaMet: 1.485 ± 0.031	3.533AlaAsn: 3.533 ± 0.049	2.173AlaPro: 2.173 ± 0.036	2.655AlaGln: 2.655 ± 0.047	2.506AlaArg: 2.506 ± 0.037	4.722AlaSer: 4.722 ± 0.06	3.574AlaThr: 3.574 ± 0.065	4.288AlaVal: 4.288 ± 0.052	0.726AlaTrp: 0.726 ± 0.021	2.563AlaTyr: 2.563 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.49CysAla: 0.49 ± 0.02	0.124CysCys: 0.124 ± 0.009	0.409CysAsp: 0.409 ± 0.017	0.516CysGlu: 0.516 ± 0.018	0.472CysPhe: 0.472 ± 0.018	0.618CysGly: 0.618 ± 0.023	0.203CysHis: 0.203 ± 0.011	0.61CysIle: 0.61 ± 0.019	0.52CysLys: 0.52 ± 0.018	0.837CysLeu: 0.837 ± 0.02	0.182CysMet: 0.182 ± 0.01	0.41CysAsn: 0.41 ± 0.017	0.381CysPro: 0.381 ± 0.018	0.304CysGln: 0.304 ± 0.013	0.317CysArg: 0.317 ± 0.014	0.615CysSer: 0.615 ± 0.03	0.502CysThr: 0.502 ± 0.033	0.451CysVal: 0.451 ± 0.017	0.102CysTrp: 0.102 ± 0.008	0.336CysTyr: 0.336 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.435AspAla: 3.435 ± 0.051	0.453AspCys: 0.453 ± 0.017	2.271AspAsp: 2.271 ± 0.044	3.212AspGlu: 3.212 ± 0.047	3.569AspPhe: 3.569 ± 0.05	3.802AspGly: 3.802 ± 0.058	0.839AspHis: 0.839 ± 0.023	3.792AspIle: 3.792 ± 0.05	3.88AspLys: 3.88 ± 0.053	5.181AspLeu: 5.181 ± 0.056	1.015AspMet: 1.015 ± 0.023	2.792AspAsn: 2.792 ± 0.04	1.947AspPro: 1.947 ± 0.037	1.602AspGln: 1.602 ± 0.031	1.927AspArg: 1.927 ± 0.036	3.098AspSer: 3.098 ± 0.049	2.3AspThr: 2.3 ± 0.038	3.066AspVal: 3.066 ± 0.047	0.843AspTrp: 0.843 ± 0.022	2.436AspTyr: 2.436 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	4.25GluAla: 4.25 ± 0.052	0.368GluCys: 0.368 ± 0.016	2.856GluAsp: 2.856 ± 0.05	4.293GluGlu: 4.293 ± 0.065	2.583GluPhe: 2.583 ± 0.037	3.769GluGly: 3.769 ± 0.046	1.019GluHis: 1.019 ± 0.024	5.136GluIle: 5.136 ± 0.062	5.73GluLys: 5.73 ± 0.075	5.644GluLeu: 5.644 ± 0.069	1.577GluMet: 1.577 ± 0.034	4.089GluAsn: 4.089 ± 0.053	1.596GluPro: 1.596 ± 0.034	2.244GluGln: 2.244 ± 0.037	2.487GluArg: 2.487 ± 0.044	3.465GluSer: 3.465 ± 0.046	3.206GluThr: 3.206 ± 0.041	4.047GluVal: 4.047 ± 0.055	0.715GluTrp: 0.715 ± 0.02	2.277GluTyr: 2.277 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.197PheAla: 3.197 ± 0.041	0.535PheCys: 0.535 ± 0.018	2.991PheAsp: 2.991 ± 0.044	3.217PheGlu: 3.217 ± 0.054	2.791PhePhe: 2.791 ± 0.046	3.687PheGly: 3.687 ± 0.048	0.87PheHis: 0.87 ± 0.021	3.511PheIle: 3.511 ± 0.052	3.268PheLys: 3.268 ± 0.045	4.998PheLeu: 4.998 ± 0.067	1.188PheMet: 1.188 ± 0.028	2.866PheAsn: 2.866 ± 0.047	1.95PhePro: 1.95 ± 0.034	1.829PheGln: 1.829 ± 0.03	2.113PheArg: 2.113 ± 0.035	4.318PheSer: 4.318 ± 0.05	3.076PheThr: 3.076 ± 0.04	3.061PheVal: 3.061 ± 0.046	0.721PheTrp: 0.721 ± 0.023	2.177PheTyr: 2.177 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	4.32GlyAla: 4.32 ± 0.065	0.716GlyCys: 0.716 ± 0.037	3.3GlyAsp: 3.3 ± 0.05	3.612GlyGlu: 3.612 ± 0.045	3.933GlyPhe: 3.933 ± 0.049	5.103GlyGly: 5.103 ± 0.085	1.101GlyHis: 1.101 ± 0.031	5.65GlyIle: 5.65 ± 0.067	5.847GlyLys: 5.847 ± 0.062	6.364GlyLeu: 6.364 ± 0.074	1.661GlyMet: 1.661 ± 0.031	4.065GlyAsn: 4.065 ± 0.064	1.377GlyPro: 1.377 ± 0.033	2.287GlyGln: 2.287 ± 0.039	2.535GlyArg: 2.535 ± 0.048	4.563GlySer: 4.563 ± 0.07	4.305GlyThr: 4.305 ± 0.072	4.399GlyVal: 4.399 ± 0.056	1.02GlyTrp: 1.02 ± 0.026	2.994GlyTyr: 2.994 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.986HisAla: 0.986 ± 0.021	0.165HisCys: 0.165 ± 0.01	0.789HisAsp: 0.789 ± 0.019	0.988HisGlu: 0.988 ± 0.029	1.228HisPhe: 1.228 ± 0.031	1.015HisGly: 1.015 ± 0.025	0.455HisHis: 0.455 ± 0.02	1.179HisIle: 1.179 ± 0.029	1.071HisLys: 1.071 ± 0.027	1.755HisLeu: 1.755 ± 0.034	0.249HisMet: 0.249 ± 0.012	0.82HisAsn: 0.82 ± 0.02	0.892HisPro: 0.892 ± 0.023	0.7HisGln: 0.7 ± 0.022	0.701HisArg: 0.701 ± 0.021	1.074HisSer: 1.074 ± 0.026	0.899HisThr: 0.899 ± 0.023	0.822HisVal: 0.822 ± 0.023	0.283HisTrp: 0.283 ± 0.013	0.762HisTyr: 0.762 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.24IleAla: 5.24 ± 0.061	0.801IleCys: 0.801 ± 0.027	4.049IleAsp: 4.049 ± 0.06	4.525IleGlu: 4.525 ± 0.061	3.574IlePhe: 3.574 ± 0.047	4.898IleGly: 4.898 ± 0.058	1.239IleHis: 1.239 ± 0.026	4.999IleIle: 4.999 ± 0.066	4.781IleLys: 4.781 ± 0.052	6.866IleLeu: 6.866 ± 0.083	1.306IleMet: 1.306 ± 0.03	4.112IleAsn: 4.112 ± 0.05	3.37IlePro: 3.37 ± 0.042	2.607IleGln: 2.607 ± 0.033	3.095IleArg: 3.095 ± 0.041	5.78IleSer: 5.78 ± 0.064	4.383IleThr: 4.383 ± 0.066	4.221IleVal: 4.221 ± 0.054	0.774IleTrp: 0.774 ± 0.02	2.629IleTyr: 2.629 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	5.292LysAla: 5.292 ± 0.064	0.401LysCys: 0.401 ± 0.017	4.149LysAsp: 4.149 ± 0.056	5.005LysGlu: 5.005 ± 0.071	2.906LysPhe: 2.906 ± 0.04	4.806LysGly: 4.806 ± 0.061	1.189LysHis: 1.189 ± 0.027	5.814LysIle: 5.814 ± 0.064	5.641LysLys: 5.641 ± 0.077	6.176LysLeu: 6.176 ± 0.069	1.946LysMet: 1.946 ± 0.034	4.624LysAsn: 4.624 ± 0.059	2.771LysPro: 2.771 ± 0.044	2.503LysGln: 2.503 ± 0.041	2.636LysArg: 2.636 ± 0.043	4.509LysSer: 4.509 ± 0.06	4.439LysThr: 4.439 ± 0.053	4.876LysVal: 4.876 ± 0.058	0.866LysTrp: 0.866 ± 0.024	2.931LysTyr: 2.931 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	6.399LeuAla: 6.399 ± 0.059	0.765LeuCys: 0.765 ± 0.024	4.549LeuAsp: 4.549 ± 0.053	5.4LeuGlu: 5.4 ± 0.074	4.773LeuPhe: 4.773 ± 0.062	5.979LeuGly: 5.979 ± 0.064	1.562LeuHis: 1.562 ± 0.033	6.745LeuIle: 6.745 ± 0.076	7.707LeuLys: 7.707 ± 0.084	9.201LeuLeu: 9.201 ± 0.097	2.16LeuMet: 2.16 ± 0.035	5.535LeuAsn: 5.535 ± 0.066	4.103LeuPro: 4.103 ± 0.047	3.369LeuGln: 3.369 ± 0.05	3.625LeuArg: 3.625 ± 0.046	7.564LeuSer: 7.564 ± 0.064	5.523LeuThr: 5.523 ± 0.056	5.212LeuVal: 5.212 ± 0.058	1.036LeuTrp: 1.036 ± 0.027	3.181LeuTyr: 3.181 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	1.596MetAla: 1.596 ± 0.033	0.146MetCys: 0.146 ± 0.009	0.976MetAsp: 0.976 ± 0.023	1.26MetGlu: 1.26 ± 0.032	0.884MetPhe: 0.884 ± 0.024	1.423MetGly: 1.423 ± 0.029	0.353MetHis: 0.353 ± 0.015	1.563MetIle: 1.563 ± 0.029	2.175MetLys: 2.175 ± 0.035	1.996MetLeu: 1.996 ± 0.034	0.577MetMet: 0.577 ± 0.02	1.327MetAsn: 1.327 ± 0.025	0.971MetPro: 0.971 ± 0.021	0.824MetGln: 0.824 ± 0.021	0.861MetArg: 0.861 ± 0.022	1.359MetSer: 1.359 ± 0.027	1.227MetThr: 1.227 ± 0.026	1.248MetVal: 1.248 ± 0.027	0.196MetTrp: 0.196 ± 0.011	0.644MetTyr: 0.644 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.939AsnAla: 3.939 ± 0.053	0.467AsnCys: 0.467 ± 0.017	2.828AsnAsp: 2.828 ± 0.046	3.092AsnGlu: 3.092 ± 0.041	2.955AsnPhe: 2.955 ± 0.048	4.219AsnGly: 4.219 ± 0.065	1.05AsnHis: 1.05 ± 0.026	4.249AsnIle: 4.249 ± 0.045	3.424AsnLys: 3.424 ± 0.043	5.445AsnLeu: 5.445 ± 0.073	1.066AsnMet: 1.066 ± 0.025	3.418AsnAsn: 3.418 ± 0.067	3.178AsnPro: 3.178 ± 0.05	2.467AsnGln: 2.467 ± 0.04	2.286AsnArg: 2.286 ± 0.038	3.598AsnSer: 3.598 ± 0.053	3.436AsnThr: 3.436 ± 0.054	3.362AsnVal: 3.362 ± 0.048	0.836AsnTrp: 0.836 ± 0.02	2.637AsnTyr: 2.637 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.9ProAla: 2.9 ± 0.048	0.224ProCys: 0.224 ± 0.012	2.595ProAsp: 2.595 ± 0.043	3.239ProGlu: 3.239 ± 0.051	1.989ProPhe: 1.989 ± 0.033	2.417ProGly: 2.417 ± 0.038	0.612ProHis: 0.612 ± 0.022	2.277ProIle: 2.277 ± 0.037	2.452ProLys: 2.452 ± 0.042	3.254ProLeu: 3.254 ± 0.041	0.739ProMet: 0.739 ± 0.023	2.127ProAsn: 2.127 ± 0.039	0.997ProPro: 0.997 ± 0.026	1.385ProGln: 1.385 ± 0.032	1.073ProArg: 1.073 ± 0.025	2.519ProSer: 2.519 ± 0.04	1.98ProThr: 1.98 ± 0.047	3.234ProVal: 3.234 ± 0.046	0.39ProTrp: 0.39 ± 0.016	1.486ProTyr: 1.486 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.428GlnAla: 2.428 ± 0.04	0.208GlnCys: 0.208 ± 0.013	1.594GlnAsp: 1.594 ± 0.032	2.359GlnGlu: 2.359 ± 0.036	1.746GlnPhe: 1.746 ± 0.028	2.012GlnGly: 2.012 ± 0.039	0.621GlnHis: 0.621 ± 0.02	2.82GlnIle: 2.82 ± 0.042	3.215GlnLys: 3.215 ± 0.05	3.459GlnLeu: 3.459 ± 0.054	0.876GlnMet: 0.876 ± 0.024	2.466GlnAsn: 2.466 ± 0.046	1.315GlnPro: 1.315 ± 0.027	1.534GlnGln: 1.534 ± 0.032	1.373GlnArg: 1.373 ± 0.027	2.326GlnSer: 2.326 ± 0.035	2.282GlnThr: 2.282 ± 0.039	2.196GlnVal: 2.196 ± 0.035	0.404GlnTrp: 0.404 ± 0.018	1.429GlnTyr: 1.429 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.239ArgAla: 2.239 ± 0.036	0.246ArgCys: 0.246 ± 0.012	1.775ArgAsp: 1.775 ± 0.032	2.441ArgGlu: 2.441 ± 0.038	2.191ArgPhe: 2.191 ± 0.038	2.169ArgGly: 2.169 ± 0.037	0.632ArgHis: 0.632 ± 0.018	3.165ArgIle: 3.165 ± 0.046	3.257ArgLys: 3.257 ± 0.048	3.735ArgLeu: 3.735 ± 0.046	0.921ArgMet: 0.921 ± 0.019	2.484ArgAsn: 2.484 ± 0.036	1.311ArgPro: 1.311 ± 0.027	1.561ArgGln: 1.561 ± 0.033	1.531ArgArg: 1.531 ± 0.031	2.089ArgSer: 2.089 ± 0.037	2.049ArgThr: 2.049 ± 0.037	2.194ArgVal: 2.194 ± 0.038	0.52ArgTrp: 0.52 ± 0.018	1.658ArgTyr: 1.658 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	4.6SerAla: 4.6 ± 0.06	0.632SerCys: 0.632 ± 0.023	3.559SerAsp: 3.559 ± 0.044	4.003SerGlu: 4.003 ± 0.052	3.882SerPhe: 3.882 ± 0.048	5.678SerGly: 5.678 ± 0.082	1.101SerHis: 1.101 ± 0.026	4.711SerIle: 4.711 ± 0.058	4.373SerLys: 4.373 ± 0.055	6.808SerLeu: 6.808 ± 0.067	1.302SerMet: 1.302 ± 0.028	3.592SerAsn: 3.592 ± 0.064	2.778SerPro: 2.778 ± 0.049	2.463SerGln: 2.463 ± 0.04	2.465SerArg: 2.465 ± 0.039	4.704SerSer: 4.704 ± 0.075	3.588SerThr: 3.588 ± 0.058	4.711SerVal: 4.711 ± 0.066	0.877SerTrp: 0.877 ± 0.023	2.795SerTyr: 2.795 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.095ThrAla: 4.095 ± 0.06	0.415ThrCys: 0.415 ± 0.022	3.187ThrAsp: 3.187 ± 0.048	3.236ThrGlu: 3.236 ± 0.046	3.053ThrPhe: 3.053 ± 0.048	4.761ThrGly: 4.761 ± 0.069	0.907ThrHis: 0.907 ± 0.023	4.248ThrIle: 4.248 ± 0.05	3.364ThrLys: 3.364 ± 0.045	5.304ThrLeu: 5.304 ± 0.056	0.957ThrMet: 0.957 ± 0.022	2.945ThrAsn: 2.945 ± 0.052	2.558ThrPro: 2.558 ± 0.042	1.887ThrGln: 1.887 ± 0.038	1.948ThrArg: 1.948 ± 0.035	3.776ThrSer: 3.776 ± 0.068	3.221ThrThr: 3.221 ± 0.079	3.743ThrVal: 3.743 ± 0.099	0.638ThrTrp: 0.638 ± 0.024	2.322ThrTyr: 2.322 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.047ValAla: 4.047 ± 0.057	0.621ValCys: 0.621 ± 0.02	2.947ValAsp: 2.947 ± 0.042	3.6ValGlu: 3.6 ± 0.049	3.292ValPhe: 3.292 ± 0.047	3.805ValGly: 3.805 ± 0.054	0.963ValHis: 0.963 ± 0.024	4.656ValIle: 4.656 ± 0.057	4.624ValLys: 4.624 ± 0.055	5.927ValLeu: 5.927 ± 0.062	1.367ValMet: 1.367 ± 0.031	3.646ValAsn: 3.646 ± 0.059	2.366ValPro: 2.366 ± 0.041	2.028ValGln: 2.028 ± 0.031	2.444ValArg: 2.444 ± 0.034	4.941ValSer: 4.941 ± 0.072	3.678ValThr: 3.678 ± 0.078	3.929ValVal: 3.929 ± 0.054	0.733ValTrp: 0.733 ± 0.02	2.331ValTyr: 2.331 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.766TrpAla: 0.766 ± 0.023	0.108TrpCys: 0.108 ± 0.008	0.659TrpAsp: 0.659 ± 0.022	0.755TrpGlu: 0.755 ± 0.022	0.625TrpPhe: 0.625 ± 0.022	0.918TrpGly: 0.918 ± 0.026	0.266TrpHis: 0.266 ± 0.012	0.841TrpIle: 0.841 ± 0.025	0.962TrpLys: 0.962 ± 0.026	1.165TrpLeu: 1.165 ± 0.03	0.339TrpMet: 0.339 ± 0.013	0.777TrpAsn: 0.777 ± 0.024	0.255TrpPro: 0.255 ± 0.015	0.59TrpGln: 0.59 ± 0.018	0.478TrpArg: 0.478 ± 0.016	0.782TrpSer: 0.782 ± 0.025	0.68TrpThr: 0.68 ± 0.025	0.769TrpVal: 0.769 ± 0.022	0.207TrpTrp: 0.207 ± 0.012	0.492TrpTyr: 0.492 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.581TyrAla: 2.581 ± 0.038	0.363TyrCys: 0.363 ± 0.014	2.172TyrAsp: 2.172 ± 0.037	2.22TyrGlu: 2.22 ± 0.041	2.407TyrPhe: 2.407 ± 0.039	2.772TyrGly: 2.772 ± 0.042	0.817TyrHis: 0.817 ± 0.023	2.344TyrIle: 2.344 ± 0.034	2.582TyrLys: 2.582 ± 0.039	3.915TyrLeu: 3.915 ± 0.054	0.695TyrMet: 0.695 ± 0.021	2.324TyrAsn: 2.324 ± 0.05	1.599TyrPro: 1.599 ± 0.029	1.824TyrGln: 1.824 ± 0.033	1.766TyrArg: 1.766 ± 0.03	2.818TyrSer: 2.818 ± 0.045	2.219TyrThr: 2.219 ± 0.04	2.117TyrVal: 2.117 ± 0.036	0.547TyrTrp: 0.547 ± 0.019	1.764TyrTyr: 1.764 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4993 proteins (1789070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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