Oikopleura dioica (Tunicate)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Tunicata; Appendicularia; Copelata; Oikopleuridae; Oikopleura

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4Y370|E4Y370_OIKDI Uncharacterized protein (Fragment) OS=Oikopleura dioica OX=34765 GN=GSOID_T00001413001 PE=4 SV=1
CC1 pKa = 7.15HH2 pKa = 7.1AFLASRR8 pKa = 11.84ALTHH12 pKa = 6.5FLAPFVATAQLAFSEE27 pKa = 4.69TDD29 pKa = 3.38TIAQKK34 pKa = 9.46TKK36 pKa = 10.74SSMKK40 pKa = 10.14IITTTTITTTNISRR54 pKa = 11.84TNATSAGQDD63 pKa = 3.21AQTEE67 pKa = 4.48LVMIAQMDD75 pKa = 4.48LLEE78 pKa = 4.59MEE80 pKa = 4.5STVLILMNVKK90 pKa = 10.38KK91 pKa = 10.61EE92 pKa = 4.23GEE94 pKa = 4.17CVEE97 pKa = 4.6NSEE100 pKa = 4.81CLNFAGGFKK109 pKa = 10.52CGEE112 pKa = 4.33CEE114 pKa = 5.36LGFTGNQTVGCFSYY128 pKa = 11.35CNGCAEE134 pKa = 4.17NAICEE139 pKa = 4.28FAEE142 pKa = 4.32DD143 pKa = 3.79TWSHH147 pKa = 6.07FCSCKK152 pKa = 10.16TGFAGNGKK160 pKa = 8.98EE161 pKa = 4.67DD162 pKa = 3.83CGKK165 pKa = 9.06DD166 pKa = 3.06TDD168 pKa = 5.39LDD170 pKa = 3.99GFPDD174 pKa = 4.06DD175 pKa = 4.71VLNCRR180 pKa = 11.84DD181 pKa = 3.55SSCRR185 pKa = 11.84ADD187 pKa = 3.45NCATIPNSGQEE198 pKa = 4.43DD199 pKa = 3.99SDD201 pKa = 4.09SDD203 pKa = 4.68GIGDD207 pKa = 4.5ACDD210 pKa = 3.59NDD212 pKa = 3.45IDD214 pKa = 4.63NDD216 pKa = 4.76GITNVNDD223 pKa = 3.28NCPRR227 pKa = 11.84LANPSQVDD235 pKa = 3.64DD236 pKa = 5.38DD237 pKa = 5.0DD238 pKa = 7.08DD239 pKa = 4.84GVGDD243 pKa = 4.18EE244 pKa = 5.41CDD246 pKa = 3.53NCPTIYY252 pKa = 10.68NPSQDD257 pKa = 3.72DD258 pKa = 3.71SCIVMDD264 pKa = 4.65SDD266 pKa = 4.49GDD268 pKa = 4.32GISDD272 pKa = 3.59EE273 pKa = 5.38DD274 pKa = 4.63DD275 pKa = 3.46NCVEE279 pKa = 4.97IPNPDD284 pKa = 3.51QLDD287 pKa = 3.57FDD289 pKa = 5.43LDD291 pKa = 4.04GLGDD295 pKa = 3.63ICDD298 pKa = 4.05VDD300 pKa = 5.54DD301 pKa = 6.59DD302 pKa = 5.36DD303 pKa = 7.53DD304 pKa = 4.58GVPDD308 pKa = 5.71DD309 pKa = 5.58VDD311 pKa = 3.77DD312 pKa = 4.56CPRR315 pKa = 11.84VYY317 pKa = 10.79NPEE320 pKa = 3.92QLGCGPDD327 pKa = 3.44SDD329 pKa = 5.69GDD331 pKa = 3.92GTPDD335 pKa = 4.52EE336 pKa = 5.74FDD338 pKa = 3.24ICPNDD343 pKa = 3.91ALISSPSFTGYY354 pKa = 8.14EE355 pKa = 4.24TILLDD360 pKa = 4.55PEE362 pKa = 5.19GSSQLDD368 pKa = 3.96PYY370 pKa = 10.39WIVLNEE376 pKa = 3.75GRR378 pKa = 11.84EE379 pKa = 4.33LIQSVNADD387 pKa = 3.45PGLAIGKK394 pKa = 9.4HH395 pKa = 4.57NVQGVDD401 pKa = 3.42YY402 pKa = 10.74SGTVYY407 pKa = 10.7INTDD411 pKa = 2.97TDD413 pKa = 3.82DD414 pKa = 4.71DD415 pKa = 4.27FVGFVFGFQSSSRR428 pKa = 11.84FYY430 pKa = 11.02SVMWKK435 pKa = 9.57QVEE438 pKa = 4.01QTYY441 pKa = 8.43WWPSPFRR448 pKa = 11.84SVAPTGLQIKK458 pKa = 9.9LVDD461 pKa = 3.78SATGPGPLLRR471 pKa = 11.84NALWHH476 pKa = 6.87GDD478 pKa = 3.67DD479 pKa = 3.87TPGQVKK485 pKa = 9.07VLWEE489 pKa = 4.43DD490 pKa = 3.47EE491 pKa = 4.38NKK493 pKa = 10.38IGWTDD498 pKa = 3.61FTAYY502 pKa = 9.89RR503 pKa = 11.84WVLHH507 pKa = 5.94HH508 pKa = 6.95RR509 pKa = 11.84PSTGLIQVQIFEE521 pKa = 4.85GEE523 pKa = 4.2TKK525 pKa = 10.23IIDD528 pKa = 3.73SGNVIDD534 pKa = 4.18TTFQGGRR541 pKa = 11.84LGVLDD546 pKa = 4.84FSQEE550 pKa = 3.76NVIWSNLNYY559 pKa = 10.13RR560 pKa = 11.84CNEE563 pKa = 3.89TMPLFF568 pKa = 4.18

Molecular weight: 61.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4X731|E4X731_OIKDI Septin OS=Oikopleura dioica OX=34765 GN=GSOID_T00003368001 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.19ISTRR6 pKa = 11.84SRR8 pKa = 11.84TTSTKK13 pKa = 10.8LPMQVLSTRR22 pKa = 11.84IQAASSRR29 pKa = 11.84SAITRR34 pKa = 11.84KK35 pKa = 9.7PPNTLVLIMTKK46 pKa = 9.79TSGFAPISRR55 pKa = 4.08

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17050

0

17050

6837326

10

8885

401.0

44.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.446 ± 0.021

2.1 ± 0.026

5.765 ± 0.016

7.396 ± 0.024

4.46 ± 0.017

5.444 ± 0.024

2.032 ± 0.008

5.829 ± 0.017

6.953 ± 0.03

8.333 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.009

4.973 ± 0.014

4.454 ± 0.019

3.982 ± 0.017

5.31 ± 0.02

7.924 ± 0.023

5.403 ± 0.023

5.441 ± 0.017

1.182 ± 0.007

2.846 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski