Acidianus filamentous virus 6

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Betalipothrixvirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7WKL0|A7WKL0_9VIRU Uncharacterized protein OS=Acidianus filamentous virus 6 OX=346882 PE=4 SV=1
MM1 pKa = 7.68SCGEE5 pKa = 4.8LPNASCTPTPNWEE18 pKa = 4.25CSDD21 pKa = 3.8VEE23 pKa = 5.31CFQQCINQPTFEE35 pKa = 4.63QFYY38 pKa = 9.5EE39 pKa = 4.38CCIQCALAHH48 pKa = 6.14YY49 pKa = 9.62IAEE52 pKa = 4.68AKK54 pKa = 10.57DD55 pKa = 3.31HH56 pKa = 5.05VTILVANAVSMIGTEE71 pKa = 4.18YY72 pKa = 10.6IPVFDD77 pKa = 3.95QMQMFPAHH85 pKa = 7.15LEE87 pKa = 3.98TLQGKK92 pKa = 7.47EE93 pKa = 3.77QVTVRR98 pKa = 11.84DD99 pKa = 3.4LVFPQNYY106 pKa = 8.08PVKK109 pKa = 10.5KK110 pKa = 7.46YY111 pKa = 8.92TYY113 pKa = 9.33YY114 pKa = 11.18EE115 pKa = 3.97NVQVNDD121 pKa = 3.06IGQPCSSPTGFCVPPGVTASEE142 pKa = 4.17EE143 pKa = 3.89LWFVNEE149 pKa = 4.03GSVVIIPSTLPPTTVSTFSYY169 pKa = 8.58ITPPPPISFQVTYY182 pKa = 7.7PTLVVPPPPPSPPPTTNILVVSKK205 pKa = 9.54PCPEE209 pKa = 3.95VCPGCICYY217 pKa = 10.68YY218 pKa = 9.68MLGIDD223 pKa = 4.76IVDD226 pKa = 3.85PTDD229 pKa = 3.12AVYY232 pKa = 10.61NYY234 pKa = 9.99QEE236 pKa = 4.3TPTGVVQQVPQNEE249 pKa = 4.36YY250 pKa = 10.98VLPSDD255 pKa = 4.12LLSIEE260 pKa = 4.42EE261 pKa = 4.58APSHH265 pKa = 4.59TTEE268 pKa = 4.11TEE270 pKa = 4.11TVEE273 pKa = 4.0EE274 pKa = 4.65SISFTLYY281 pKa = 10.59YY282 pKa = 10.73VVTTTLPVLDD292 pKa = 4.17VNFVYY297 pKa = 10.03PVTLIRR303 pKa = 11.84EE304 pKa = 4.47VEE306 pKa = 4.32SVTASRR312 pKa = 11.84SIQTVYY318 pKa = 10.48FSYY321 pKa = 10.35PVSKK325 pKa = 10.43QVFSEE330 pKa = 4.45YY331 pKa = 8.61VTASSISVSAYY342 pKa = 7.48FQPIVKK348 pKa = 9.84LVQEE352 pKa = 4.47SEE354 pKa = 4.6VTVSSIPILQIKK366 pKa = 7.01FTKK369 pKa = 7.93PTIVIQSVPTSVSQILLVADD389 pKa = 4.93MIRR392 pKa = 11.84TITPEE397 pKa = 3.86LVSEE401 pKa = 4.19YY402 pKa = 10.3TSAIKK407 pKa = 10.02PIIEE411 pKa = 4.26MIAYY415 pKa = 9.71SVVTEE420 pKa = 3.99VLKK423 pKa = 11.02SVSVSASEE431 pKa = 4.14EE432 pKa = 3.98FLISTVYY439 pKa = 10.52EE440 pKa = 4.49VVQKK444 pKa = 11.05ALVPIYY450 pKa = 10.01TSASEE455 pKa = 4.2EE456 pKa = 3.93FLNTVIYY463 pKa = 7.48EE464 pKa = 4.35TVKK467 pKa = 10.79EE468 pKa = 4.38VVTSLKK474 pKa = 8.05TTQSEE479 pKa = 4.17ITMVSYY485 pKa = 10.74VQEE488 pKa = 4.38KK489 pKa = 8.1VTPTQFSISSSQSEE503 pKa = 4.5TTMVSYY509 pKa = 10.55VQEE512 pKa = 4.92KK513 pKa = 8.48ITPTQLPVSSSQSEE527 pKa = 4.55GIMVSSVYY535 pKa = 9.11QQITAVVVPEE545 pKa = 4.41TTTSPAEE552 pKa = 4.2TIVNAIISCPFVDD565 pKa = 5.91AKK567 pKa = 10.13NQCYY571 pKa = 6.33TTGYY575 pKa = 9.1TIYY578 pKa = 10.71LFNNTSTTIPSGYY591 pKa = 9.21PIPIWIYY598 pKa = 10.1VDD600 pKa = 3.87GANSTYY606 pKa = 11.15SNVRR610 pKa = 11.84LSYY613 pKa = 10.83NGNEE617 pKa = 4.03LYY619 pKa = 9.75TWVEE623 pKa = 4.2EE624 pKa = 4.27IVGGSALMWVVLPTDD639 pKa = 4.41LSTVIQIDD647 pKa = 3.51VWVGDD652 pKa = 3.51SSFVYY657 pKa = 10.49DD658 pKa = 4.58GVAGAYY664 pKa = 9.51KK665 pKa = 10.37EE666 pKa = 4.17IAGCSNDD673 pKa = 3.44NGSKK677 pKa = 10.23IFTLYY682 pKa = 11.28DD683 pKa = 3.64NFCGTSLNTDD693 pKa = 2.36IWNLYY698 pKa = 9.26VSGDD702 pKa = 3.38YY703 pKa = 10.73SYY705 pKa = 11.52EE706 pKa = 4.06VNNDD710 pKa = 3.95LIINVSQGQTGYY722 pKa = 11.13VVLQSKK728 pKa = 7.27QTFGEE733 pKa = 4.85GYY735 pKa = 8.49MFEE738 pKa = 4.49VMSSTSNFITNIRR751 pKa = 11.84TAQPSISVGNGAEE764 pKa = 4.05YY765 pKa = 11.0AFTDD769 pKa = 5.3GIISSSSPQEE779 pKa = 3.78TADD782 pKa = 3.7FSINGNCVPYY792 pKa = 10.59YY793 pKa = 10.15VVEE796 pKa = 4.86LGTQSLTCPSSCPSSSSSFCEE817 pKa = 3.73TSYY820 pKa = 10.45ATYY823 pKa = 10.86AVIGINYY830 pKa = 7.75PFRR833 pKa = 11.84KK834 pKa = 9.88LISGLFYY841 pKa = 10.93QNNNVALVVDD851 pKa = 4.43RR852 pKa = 11.84VMLVNSLPSQSISGNVYY869 pKa = 8.92MYY871 pKa = 11.07LGTAYY876 pKa = 10.86SDD878 pKa = 3.26ATGNLTLTYY887 pKa = 9.84HH888 pKa = 6.79FARR891 pKa = 11.84VYY893 pKa = 10.99PMIAPPFPASQSGYY907 pKa = 9.94IVDD910 pKa = 4.86SYY912 pKa = 11.54WEE914 pKa = 4.23FNGSSSYY921 pKa = 10.23MVVEE925 pKa = 4.7LPQVSSSSHH934 pKa = 4.63TFNFTVTYY942 pKa = 10.04AVWFRR947 pKa = 11.84TTSGTGMILSDD958 pKa = 3.84NQSASSPCYY967 pKa = 10.14DD968 pKa = 3.95YY969 pKa = 11.96YY970 pKa = 11.83NDD972 pKa = 3.88ILSIVNGKK980 pKa = 8.97LVAGYY985 pKa = 9.27CCNGTFVTTSDD996 pKa = 3.92TYY998 pKa = 11.94NDD1000 pKa = 4.47GNWHH1004 pKa = 5.89FAVVILDD1011 pKa = 3.71PSNNSITLYY1020 pKa = 10.97VDD1022 pKa = 3.19GQEE1025 pKa = 4.17IGSATVSSSLEE1036 pKa = 3.95PPNPYY1041 pKa = 10.09YY1042 pKa = 10.93LWIGYY1047 pKa = 9.26GSSCAGWADD1056 pKa = 3.79EE1057 pKa = 5.27GISGYY1062 pKa = 10.9FNGDD1066 pKa = 3.47IAWVAVFNTALSSSQIQEE1084 pKa = 4.0IYY1086 pKa = 10.99QMGPYY1091 pKa = 10.22SIDD1094 pKa = 3.23PLLFTDD1100 pKa = 5.8NIVGWWTPWNGPPDD1114 pKa = 4.64LSTADD1119 pKa = 4.98DD1120 pKa = 3.68SLTYY1124 pKa = 10.69YY1125 pKa = 10.79NISTPSFVGYY1135 pKa = 10.21VGVYY1139 pKa = 8.42PAIDD1143 pKa = 3.48AFYY1146 pKa = 10.86SDD1148 pKa = 3.77ARR1150 pKa = 11.84TTFVLYY1156 pKa = 8.7NTYY1159 pKa = 10.69SSLMVTLTSPTTGQYY1174 pKa = 10.77GGVIFSYY1181 pKa = 10.5SYY1183 pKa = 8.8TQGNVFTVSGLFTLDD1198 pKa = 3.9NPSSCPADD1206 pKa = 3.22GFAFAIFSSGFDD1218 pKa = 3.14TSNIVVPSISGCKK1231 pKa = 9.01FQGSMSIPEE1240 pKa = 4.26TSSPTILVQYY1250 pKa = 9.89DD1251 pKa = 3.99PYY1253 pKa = 10.31WAEE1256 pKa = 3.91PCAGNQVGTGVNISVVNGTSVTEE1279 pKa = 4.12IVCGAGSGTFNTNVQSGNEE1298 pKa = 3.68MFYY1301 pKa = 9.97IVSYY1305 pKa = 10.25IPSSNEE1311 pKa = 3.02VCYY1314 pKa = 10.6TMFFIPNNQFVTGCTSLGSDD1334 pKa = 4.15FSAPSSGTYY1343 pKa = 9.87YY1344 pKa = 10.72LAVAGATGGQYY1355 pKa = 10.74AQWLFDD1361 pKa = 3.42ARR1363 pKa = 11.84TLHH1366 pKa = 7.08ISQQ1369 pKa = 4.07

Molecular weight: 149.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7WKJ2|A7WKJ2_9VIRU Uncharacterized protein OS=Acidianus filamentous virus 6 OX=346882 PE=4 SV=1
MM1 pKa = 7.16MKK3 pKa = 8.65ITKK6 pKa = 6.76TTFVRR11 pKa = 11.84LCFWALSQARR21 pKa = 11.84MSGIKK26 pKa = 9.55VVPRR30 pKa = 11.84IMLRR34 pKa = 11.84FLDD37 pKa = 4.06EE38 pKa = 4.33VKK40 pKa = 10.66ASPRR44 pKa = 11.84TKK46 pKa = 10.47EE47 pKa = 3.91RR48 pKa = 11.84LLLLIDD54 pKa = 4.24EE55 pKa = 5.49LDD57 pKa = 3.53TGKK60 pKa = 8.47ITPRR64 pKa = 11.84EE65 pKa = 3.71FARR68 pKa = 11.84RR69 pKa = 11.84LFLEE73 pKa = 4.31YY74 pKa = 9.92PRR76 pKa = 11.84PTRR79 pKa = 11.84KK80 pKa = 9.94GRR82 pKa = 11.84VLSLKK87 pKa = 8.45EE88 pKa = 4.02FKK90 pKa = 10.57RR91 pKa = 11.84ITSLGKK97 pKa = 10.09

Molecular weight: 11.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11980

49

1369

181.5

20.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.925 ± 0.238

1.377 ± 0.167

4.624 ± 0.232

7.429 ± 0.451

4.416 ± 0.173

5.242 ± 0.22

1.311 ± 0.162

8.865 ± 0.4

7.321 ± 0.724

8.664 ± 0.467

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.158

5.192 ± 0.223

3.973 ± 0.355

3.18 ± 0.243

3.681 ± 0.393

6.619 ± 0.722

5.985 ± 0.461

7.98 ± 0.427

1.018 ± 0.104

5.735 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski