Amino acid dipepetide frequency for beta proteobacterium MWH-P2sevCIIIb

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.958AlaAla: 11.958 ± 0.147	1.201AlaCys: 1.201 ± 0.04	5.451AlaAsp: 5.451 ± 0.08	5.378AlaGlu: 5.378 ± 0.089	3.683AlaPhe: 3.683 ± 0.068	8.688AlaGly: 8.688 ± 0.124	2.14AlaHis: 2.14 ± 0.057	6.13AlaIle: 6.13 ± 0.09	4.917AlaLys: 4.917 ± 0.08	11.874AlaLeu: 11.874 ± 0.124	3.109AlaMet: 3.109 ± 0.063	3.479AlaAsn: 3.479 ± 0.065	4.087AlaPro: 4.087 ± 0.077	4.824AlaGln: 4.824 ± 0.089	6.015AlaArg: 6.015 ± 0.098	6.443AlaSer: 6.443 ± 0.109	5.699AlaThr: 5.699 ± 0.102	7.57AlaVal: 7.57 ± 0.104	1.484AlaTrp: 1.484 ± 0.045	2.549AlaTyr: 2.549 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.067CysAla: 1.067 ± 0.038	0.138CysCys: 0.138 ± 0.012	0.514CysAsp: 0.514 ± 0.025	0.516CysGlu: 0.516 ± 0.024	0.41CysPhe: 0.41 ± 0.021	0.954CysGly: 0.954 ± 0.035	0.267CysHis: 0.267 ± 0.016	0.46CysIle: 0.46 ± 0.021	0.326CysLys: 0.326 ± 0.021	0.98CysLeu: 0.98 ± 0.034	0.215CysMet: 0.215 ± 0.014	0.32CysAsn: 0.32 ± 0.019	0.507CysPro: 0.507 ± 0.024	0.348CysGln: 0.348 ± 0.02	0.499CysArg: 0.499 ± 0.023	0.591CysSer: 0.591 ± 0.026	0.486CysThr: 0.486 ± 0.021	0.822CysVal: 0.822 ± 0.029	0.125CysTrp: 0.125 ± 0.012	0.241CysTyr: 0.241 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.303AspAla: 5.303 ± 0.071	0.514AspCys: 0.514 ± 0.024	2.537AspAsp: 2.537 ± 0.053	3.047AspGlu: 3.047 ± 0.064	2.247AspPhe: 2.247 ± 0.052	3.855AspGly: 3.855 ± 0.069	1.23AspHis: 1.23 ± 0.041	3.091AspIle: 3.091 ± 0.063	2.242AspLys: 2.242 ± 0.049	5.822AspLeu: 5.822 ± 0.085	1.362AspMet: 1.362 ± 0.039	1.388AspAsn: 1.388 ± 0.037	2.825AspPro: 2.825 ± 0.059	2.224AspGln: 2.224 ± 0.047	3.05AspArg: 3.05 ± 0.064	2.844AspSer: 2.844 ± 0.052	2.564AspThr: 2.564 ± 0.052	4.012AspVal: 4.012 ± 0.071	0.951AspTrp: 0.951 ± 0.029	1.555AspTyr: 1.555 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.482GluAla: 5.482 ± 0.086	0.362GluCys: 0.362 ± 0.022	2.253GluAsp: 2.253 ± 0.05	2.227GluGlu: 2.227 ± 0.059	1.901GluPhe: 1.901 ± 0.046	3.329GluGly: 3.329 ± 0.055	1.292GluHis: 1.292 ± 0.041	3.437GluIle: 3.437 ± 0.063	2.319GluLys: 2.319 ± 0.06	5.503GluLeu: 5.503 ± 0.081	1.356GluMet: 1.356 ± 0.034	1.628GluAsn: 1.628 ± 0.041	2.21GluPro: 2.21 ± 0.05	2.786GluGln: 2.786 ± 0.072	3.285GluArg: 3.285 ± 0.064	2.914GluSer: 2.914 ± 0.055	2.779GluThr: 2.779 ± 0.056	3.758GluVal: 3.758 ± 0.077	0.654GluTrp: 0.654 ± 0.028	1.185GluTyr: 1.185 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	4.211PheAla: 4.211 ± 0.075	0.47PheCys: 0.47 ± 0.021	2.652PheAsp: 2.652 ± 0.056	2.253PheGlu: 2.253 ± 0.047	1.576PhePhe: 1.576 ± 0.047	3.508PheGly: 3.508 ± 0.06	0.694PheHis: 0.694 ± 0.028	2.1PheIle: 2.1 ± 0.044	1.727PheLys: 1.727 ± 0.051	3.295PheLeu: 3.295 ± 0.068	0.914PheMet: 0.914 ± 0.033	1.522PheAsn: 1.522 ± 0.036	1.571PhePro: 1.571 ± 0.043	1.151PheGln: 1.151 ± 0.032	1.598PheArg: 1.598 ± 0.041	2.626PheSer: 2.626 ± 0.055	2.066PheThr: 2.066 ± 0.043	2.947PheVal: 2.947 ± 0.069	0.546PheTrp: 0.546 ± 0.025	1.057PheTyr: 1.057 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	7.291GlyAla: 7.291 ± 0.126	0.91GlyCys: 0.91 ± 0.025	3.595GlyAsp: 3.595 ± 0.067	3.621GlyGlu: 3.621 ± 0.069	3.481GlyPhe: 3.481 ± 0.058	6.168GlyGly: 6.168 ± 0.211	1.851GlyHis: 1.851 ± 0.044	4.64GlyIle: 4.64 ± 0.072	3.683GlyLys: 3.683 ± 0.079	8.846GlyLeu: 8.846 ± 0.094	2.296GlyMet: 2.296 ± 0.053	2.389GlyAsn: 2.389 ± 0.08	2.618GlyPro: 2.618 ± 0.055	3.339GlyGln: 3.339 ± 0.064	4.224GlyArg: 4.224 ± 0.067	4.966GlySer: 4.966 ± 0.162	4.192GlyThr: 4.192 ± 0.126	6.145GlyVal: 6.145 ± 0.096	1.277GlyTrp: 1.277 ± 0.036	2.43GlyTyr: 2.43 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	2.24HisAla: 2.24 ± 0.05	0.264HisCys: 0.264 ± 0.016	1.134HisAsp: 1.134 ± 0.034	1.136HisGlu: 1.136 ± 0.04	0.938HisPhe: 0.938 ± 0.033	1.79HisGly: 1.79 ± 0.045	0.658HisHis: 0.658 ± 0.028	1.177HisIle: 1.177 ± 0.034	0.644HisLys: 0.644 ± 0.026	2.263HisLeu: 2.263 ± 0.052	0.496HisMet: 0.496 ± 0.021	0.596HisAsn: 0.596 ± 0.026	1.426HisPro: 1.426 ± 0.044	0.934HisGln: 0.934 ± 0.031	1.201HisArg: 1.201 ± 0.041	1.172HisSer: 1.172 ± 0.038	1.108HisThr: 1.108 ± 0.035	1.512HisVal: 1.512 ± 0.043	0.342HisTrp: 0.342 ± 0.018	0.66HisTyr: 0.66 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.013IleAla: 7.013 ± 0.094	0.62IleCys: 0.62 ± 0.026	3.93IleAsp: 3.93 ± 0.067	3.897IleGlu: 3.897 ± 0.061	1.993IlePhe: 1.993 ± 0.048	4.763IleGly: 4.763 ± 0.083	1.077IleHis: 1.077 ± 0.035	2.65IleIle: 2.65 ± 0.05	2.764IleLys: 2.764 ± 0.049	4.961IleLeu: 4.961 ± 0.085	1.223IleMet: 1.223 ± 0.034	2.258IleAsn: 2.258 ± 0.046	2.76IlePro: 2.76 ± 0.053	1.958IleGln: 1.958 ± 0.042	2.801IleArg: 2.801 ± 0.055	3.702IleSer: 3.702 ± 0.066	3.182IleThr: 3.182 ± 0.074	4.486IleVal: 4.486 ± 0.077	0.679IleTrp: 0.679 ± 0.029	1.378IleTyr: 1.378 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.415LysAla: 4.415 ± 0.086	0.247LysCys: 0.247 ± 0.016	2.134LysAsp: 2.134 ± 0.041	2.079LysGlu: 2.079 ± 0.056	1.413LysPhe: 1.413 ± 0.044	2.959LysGly: 2.959 ± 0.06	0.979LysHis: 0.979 ± 0.031	2.66LysIle: 2.66 ± 0.056	2.191LysLys: 2.191 ± 0.063	4.723LysLeu: 4.723 ± 0.083	1.23LysMet: 1.23 ± 0.037	1.527LysAsn: 1.527 ± 0.04	2.466LysPro: 2.466 ± 0.049	2.008LysGln: 2.008 ± 0.049	2.682LysArg: 2.682 ± 0.051	2.608LysSer: 2.608 ± 0.06	2.732LysThr: 2.732 ± 0.05	3.066LysVal: 3.066 ± 0.063	0.566LysTrp: 0.566 ± 0.023	0.992LysTyr: 0.992 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	12.141LeuAla: 12.141 ± 0.125	1.102LeuCys: 1.102 ± 0.037	5.677LeuAsp: 5.677 ± 0.086	4.888LeuGlu: 4.888 ± 0.073	3.862LeuPhe: 3.862 ± 0.089	8.267LeuGly: 8.267 ± 0.099	2.102LeuHis: 2.102 ± 0.056	6.361LeuIle: 6.361 ± 0.087	4.89LeuLys: 4.89 ± 0.085	10.609LeuLeu: 10.609 ± 0.16	2.643LeuMet: 2.643 ± 0.061	3.67LeuAsn: 3.67 ± 0.059	5.257LeuPro: 5.257 ± 0.079	3.703LeuGln: 3.703 ± 0.063	5.654LeuArg: 5.654 ± 0.092	7.502LeuSer: 7.502 ± 0.102	6.41LeuThr: 6.41 ± 0.098	7.534LeuVal: 7.534 ± 0.106	1.098LeuTrp: 1.098 ± 0.041	2.042LeuTyr: 2.042 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.826MetAla: 2.826 ± 0.06	0.174MetCys: 0.174 ± 0.013	1.177MetAsp: 1.177 ± 0.038	0.863MetGlu: 0.863 ± 0.027	0.896MetPhe: 0.896 ± 0.029	2.039MetGly: 2.039 ± 0.047	0.514MetHis: 0.514 ± 0.022	1.505MetIle: 1.505 ± 0.042	1.227MetLys: 1.227 ± 0.036	2.82MetLeu: 2.82 ± 0.062	0.785MetMet: 0.785 ± 0.032	1.011MetAsn: 1.011 ± 0.035	1.44MetPro: 1.44 ± 0.041	1.144MetGln: 1.144 ± 0.033	1.591MetArg: 1.591 ± 0.041	2.012MetSer: 2.012 ± 0.044	1.955MetThr: 1.955 ± 0.044	1.797MetVal: 1.797 ± 0.047	0.199MetTrp: 0.199 ± 0.016	0.468MetTyr: 0.468 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.329AsnAla: 3.329 ± 0.061	0.312AsnCys: 0.312 ± 0.016	1.573AsnAsp: 1.573 ± 0.042	1.629AsnGlu: 1.629 ± 0.036	1.229AsnPhe: 1.229 ± 0.036	2.491AsnGly: 2.491 ± 0.083	0.717AsnHis: 0.717 ± 0.027	1.986AsnIle: 1.986 ± 0.048	1.483AsnLys: 1.483 ± 0.041	3.429AsnLeu: 3.429 ± 0.065	0.896AsnMet: 0.896 ± 0.032	1.159AsnAsn: 1.159 ± 0.047	2.256AsnPro: 2.256 ± 0.049	1.457AsnGln: 1.457 ± 0.037	1.882AsnArg: 1.882 ± 0.05	1.777AsnSer: 1.777 ± 0.063	1.873AsnThr: 1.873 ± 0.075	2.341AsnVal: 2.341 ± 0.06	0.513AsnTrp: 0.513 ± 0.027	0.964AsnTyr: 0.964 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.873ProAla: 4.873 ± 0.077	0.353ProCys: 0.353 ± 0.021	3.044ProAsp: 3.044 ± 0.055	3.114ProGlu: 3.114 ± 0.053	1.842ProPhe: 1.842 ± 0.044	3.661ProGly: 3.661 ± 0.061	1.03ProHis: 1.03 ± 0.029	2.585ProIle: 2.585 ± 0.052	2.014ProLys: 2.014 ± 0.043	4.455ProLeu: 4.455 ± 0.071	1.162ProMet: 1.162 ± 0.038	1.617ProAsn: 1.617 ± 0.04	1.786ProPro: 1.786 ± 0.05	1.721ProGln: 1.721 ± 0.04	2.091ProArg: 2.091 ± 0.053	2.824ProSer: 2.824 ± 0.051	2.597ProThr: 2.597 ± 0.052	3.887ProVal: 3.887 ± 0.057	0.673ProTrp: 0.673 ± 0.032	1.363ProTyr: 1.363 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	5.176GlnAla: 5.176 ± 0.08	0.349GlnCys: 0.349 ± 0.02	1.864GlnAsp: 1.864 ± 0.042	1.659GlnGlu: 1.659 ± 0.046	1.461GlnPhe: 1.461 ± 0.035	2.856GlnGly: 2.856 ± 0.054	0.984GlnHis: 0.984 ± 0.034	2.615GlnIle: 2.615 ± 0.049	1.591GlnLys: 1.591 ± 0.038	4.059GlnLeu: 4.059 ± 0.075	1.129GlnMet: 1.129 ± 0.034	1.306GlnAsn: 1.306 ± 0.042	1.881GlnPro: 1.881 ± 0.043	2.01GlnGln: 2.01 ± 0.052	2.467GlnArg: 2.467 ± 0.051	2.588GlnSer: 2.588 ± 0.054	2.599GlnThr: 2.599 ± 0.047	3.02GlnVal: 3.02 ± 0.064	0.599GlnTrp: 0.599 ± 0.026	1.083GlnTyr: 1.083 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	5.446ArgAla: 5.446 ± 0.081	0.493ArgCys: 0.493 ± 0.023	2.935ArgAsp: 2.935 ± 0.053	3.047ArgGlu: 3.047 ± 0.062	2.254ArgPhe: 2.254 ± 0.047	3.532ArgGly: 3.532 ± 0.056	1.311ArgHis: 1.311 ± 0.039	3.392ArgIle: 3.392 ± 0.06	2.216ArgLys: 2.216 ± 0.045	6.178ArgLeu: 6.178 ± 0.092	1.575ArgMet: 1.575 ± 0.044	1.808ArgAsn: 1.808 ± 0.041	2.439ArgPro: 2.439 ± 0.043	2.508ArgGln: 2.508 ± 0.059	3.14ArgArg: 3.14 ± 0.069	3.089ArgSer: 3.089 ± 0.066	2.636ArgThr: 2.636 ± 0.061	4.137ArgVal: 4.137 ± 0.071	0.82ArgTrp: 0.82 ± 0.028	1.677ArgTyr: 1.677 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.417SerAla: 6.417 ± 0.125	0.51SerCys: 0.51 ± 0.024	3.111SerAsp: 3.111 ± 0.057	2.947SerGlu: 2.947 ± 0.055	2.638SerPhe: 2.638 ± 0.041	5.635SerGly: 5.635 ± 0.201	1.292SerHis: 1.292 ± 0.042	3.651SerIle: 3.651 ± 0.064	2.525SerLys: 2.525 ± 0.05	6.784SerLeu: 6.784 ± 0.086	1.653SerMet: 1.653 ± 0.044	2.106SerAsn: 2.106 ± 0.074	2.773SerPro: 2.773 ± 0.054	2.414SerGln: 2.414 ± 0.053	3.226SerArg: 3.226 ± 0.061	4.248SerSer: 4.248 ± 0.174	3.508SerThr: 3.508 ± 0.083	4.726SerVal: 4.726 ± 0.074	0.869SerTrp: 0.869 ± 0.03	1.554SerTyr: 1.554 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	5.733ThrAla: 5.733 ± 0.098	0.453ThrCys: 0.453 ± 0.022	2.929ThrAsp: 2.929 ± 0.056	2.834ThrGlu: 2.834 ± 0.055	1.961ThrPhe: 1.961 ± 0.048	4.91ThrGly: 4.91 ± 0.09	1.248ThrHis: 1.248 ± 0.037	3.25ThrIle: 3.25 ± 0.072	2.135ThrLys: 2.135 ± 0.052	6.232ThrLeu: 6.232 ± 0.1	1.315ThrMet: 1.315 ± 0.04	1.78ThrAsn: 1.78 ± 0.063	3.031ThrPro: 3.031 ± 0.056	2.275ThrGln: 2.275 ± 0.047	2.921ThrArg: 2.921 ± 0.06	3.373ThrSer: 3.373 ± 0.101	3.387ThrThr: 3.387 ± 0.115	4.239ThrVal: 4.239 ± 0.078	0.776ThrTrp: 0.776 ± 0.032	1.328ThrTyr: 1.328 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	8.069ValAla: 8.069 ± 0.099	0.831ValCys: 0.831 ± 0.031	4.082ValAsp: 4.082 ± 0.071	3.821ValGlu: 3.821 ± 0.072	2.959ValPhe: 2.959 ± 0.057	5.425ValGly: 5.425 ± 0.081	1.478ValHis: 1.478 ± 0.042	4.534ValIle: 4.534 ± 0.08	3.342ValLys: 3.342 ± 0.071	7.906ValLeu: 7.906 ± 0.099	2.165ValMet: 2.165 ± 0.049	2.614ValAsn: 2.614 ± 0.073	3.358ValPro: 3.358 ± 0.058	2.568ValGln: 2.568 ± 0.045	3.83ValArg: 3.83 ± 0.067	4.931ValSer: 4.931 ± 0.084	4.359ValThr: 4.359 ± 0.078	6.088ValVal: 6.088 ± 0.097	0.899ValTrp: 0.899 ± 0.03	1.667ValTyr: 1.667 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.148TrpAla: 1.148 ± 0.039	0.168TrpCys: 0.168 ± 0.012	0.675TrpAsp: 0.675 ± 0.028	0.452TrpGlu: 0.452 ± 0.022	0.635TrpPhe: 0.635 ± 0.026	0.93TrpGly: 0.93 ± 0.033	0.352TrpHis: 0.352 ± 0.019	0.823TrpIle: 0.823 ± 0.032	0.476TrpLys: 0.476 ± 0.023	1.948TrpLeu: 1.948 ± 0.056	0.421TrpMet: 0.421 ± 0.018	0.368TrpAsn: 0.368 ± 0.019	0.64TrpPro: 0.64 ± 0.028	0.73TrpGln: 0.73 ± 0.031	0.929TrpArg: 0.929 ± 0.034	0.815TrpSer: 0.815 ± 0.029	0.64TrpThr: 0.64 ± 0.026	1.011TrpVal: 1.011 ± 0.035	0.228TrpTrp: 0.228 ± 0.015	0.324TrpTyr: 0.324 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.541TyrAla: 2.541 ± 0.05	0.312TyrCys: 0.312 ± 0.018	1.397TyrAsp: 1.397 ± 0.037	1.255TyrGlu: 1.255 ± 0.039	1.106TyrPhe: 1.106 ± 0.039	2.204TyrGly: 2.204 ± 0.055	0.477TyrHis: 0.477 ± 0.02	1.049TyrIle: 1.049 ± 0.031	0.964TyrLys: 0.964 ± 0.031	2.723TyrLeu: 2.723 ± 0.05	0.499TyrMet: 0.499 ± 0.021	0.748TyrAsn: 0.748 ± 0.029	1.363TyrPro: 1.363 ± 0.039	1.191TyrGln: 1.191 ± 0.04	1.548TyrArg: 1.548 ± 0.038	1.558TyrSer: 1.558 ± 0.049	1.34TyrThr: 1.34 ± 0.039	1.829TyrVal: 1.829 ± 0.043	0.415TyrTrp: 0.415 ± 0.019	0.679TyrTyr: 0.679 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2981 proteins (983088 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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