Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker s yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces cerevisiae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P40544|YKE4_YEAST Zinc transporter YKE4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKE4 PE=1 SV=1
MM1 pKa = 7.68LIDD4 pKa = 3.84QIVAAVPGVPDD15 pKa = 3.41ISGNASALSLQRR27 pKa = 11.84RR28 pKa = 11.84SQEE31 pKa = 4.06FATSWISMTYY41 pKa = 8.02SQSYY45 pKa = 10.48GDD47 pKa = 5.81LAQNWQNDD55 pKa = 3.25EE56 pKa = 4.14GGAGNFDD63 pKa = 3.83SYY65 pKa = 12.01AEE67 pKa = 4.11EE68 pKa = 3.99KK69 pKa = 10.39LQNFFSGNRR78 pKa = 11.84DD79 pKa = 2.74WKK81 pKa = 10.0YY82 pKa = 11.13CFAAEE87 pKa = 4.02DD88 pKa = 4.06TKK90 pKa = 11.35NGEE93 pKa = 4.07PLDD96 pKa = 4.22YY97 pKa = 11.09DD98 pKa = 5.51DD99 pKa = 5.99IPGDD103 pKa = 3.75GAGTGSAFKK112 pKa = 11.04SEE114 pKa = 4.71IYY116 pKa = 10.46FNTYY120 pKa = 10.14GGIDD124 pKa = 3.49NYY126 pKa = 11.27CNDD129 pKa = 3.72EE130 pKa = 4.79HH131 pKa = 9.1IGAQNTGDD139 pKa = 3.51GRR141 pKa = 3.85

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15731|UBC4_YEAST Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UBC4 PE=1 SV=1
MRAKWRKKRTRRLKRKRRKVRARSK

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6050

29

6079

2949596

16

4910

485.2

54.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.493 ± 0.026

1.269 ± 0.012

5.837 ± 0.021

6.523 ± 0.033

4.435 ± 0.022

4.971 ± 0.031

2.172 ± 0.014

6.557 ± 0.023

7.342 ± 0.03

9.507 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.011

6.156 ± 0.029

4.38 ± 0.022

3.95 ± 0.023

4.444 ± 0.021

8.981 ± 0.04

5.913 ± 0.031

5.558 ± 0.021

1.041 ± 0.009

3.385 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski