Blastocystis hominis
Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Opalinata; Blastocystidae; Blastocystis
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Virtual 2D-PAGE plot for 5820 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D8LZ01|D8LZ01_BLAHO Uncharacterized protein OS=Blastocystis hominis OX=12968 GN=GSBLH_T00006233001 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 3.93NAIAGFCSVTGTDD15 pKa = 3.31AEE17 pKa = 4.41TARR20 pKa = 11.84KK21 pKa = 9.2YY22 pKa = 11.2LEE24 pKa = 4.05ISEE27 pKa = 4.93GNIDD31 pKa = 3.77QAISLYY37 pKa = 10.39FDD39 pKa = 3.48NANIFSDD46 pKa = 4.04HH47 pKa = 6.67AASAQDD53 pKa = 3.39NDD55 pKa = 4.14SPSSIEE61 pKa = 3.68FDD63 pKa = 3.22EE64 pKa = 5.49DD65 pKa = 3.73EE66 pKa = 4.89NSMEE70 pKa = 4.08MEE72 pKa = 3.8EE73 pKa = 4.18MGRR76 pKa = 11.84SNRR79 pKa = 11.84IYY81 pKa = 10.13EE82 pKa = 4.03NQEE85 pKa = 3.08IYY87 pKa = 10.06IPKK90 pKa = 10.35GSDD93 pKa = 2.73IQKK96 pKa = 9.14YY97 pKa = 9.46SVSIFDD103 pKa = 3.74NAEE106 pKa = 3.92SDD108 pKa = 3.61SSGYY112 pKa = 9.44CAHH115 pKa = 6.84FMM117 pKa = 4.98
MM1 pKa = 8.02DD2 pKa = 3.93NAIAGFCSVTGTDD15 pKa = 3.31AEE17 pKa = 4.41TARR20 pKa = 11.84KK21 pKa = 9.2YY22 pKa = 11.2LEE24 pKa = 4.05ISEE27 pKa = 4.93GNIDD31 pKa = 3.77QAISLYY37 pKa = 10.39FDD39 pKa = 3.48NANIFSDD46 pKa = 4.04HH47 pKa = 6.67AASAQDD53 pKa = 3.39NDD55 pKa = 4.14SPSSIEE61 pKa = 3.68FDD63 pKa = 3.22EE64 pKa = 5.49DD65 pKa = 3.73EE66 pKa = 4.89NSMEE70 pKa = 4.08MEE72 pKa = 3.8EE73 pKa = 4.18MGRR76 pKa = 11.84SNRR79 pKa = 11.84IYY81 pKa = 10.13EE82 pKa = 4.03NQEE85 pKa = 3.08IYY87 pKa = 10.06IPKK90 pKa = 10.35GSDD93 pKa = 2.73IQKK96 pKa = 9.14YY97 pKa = 9.46SVSIFDD103 pKa = 3.74NAEE106 pKa = 3.92SDD108 pKa = 3.61SSGYY112 pKa = 9.44CAHH115 pKa = 6.84FMM117 pKa = 4.98
Molecular weight: 13.02 kDa
Isoelectric point according different methods:
Protein with the highest isoelectric point:
>tr|D8M6C3|D8M6C3_BLAHO PAPS_reduct domain-containing protein OS=Blastocystis hominis OX=12968 GN=GSBLH_T00003505001 PE=4 SV=1
MM1 pKa = 6.42TTTRR5 pKa = 11.84SRR7 pKa = 11.84GSDD10 pKa = 2.78ATSRR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TRR21 pKa = 11.84WRR23 pKa = 11.84SSWIRR28 pKa = 11.84GMMCRR33 pKa = 11.84SRR35 pKa = 11.84SKK37 pKa = 10.23RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.26RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.63KK48 pKa = 8.89EE49 pKa = 3.67RR50 pKa = 11.84KK51 pKa = 8.78KK52 pKa = 9.51KK53 pKa = 8.8RR54 pKa = 11.84KK55 pKa = 8.98RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84TSRR61 pKa = 11.84AGRR64 pKa = 11.84RR65 pKa = 11.84VSRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84WRR72 pKa = 11.84QRR74 pKa = 2.75
MM1 pKa = 6.42TTTRR5 pKa = 11.84SRR7 pKa = 11.84GSDD10 pKa = 2.78ATSRR14 pKa = 11.84RR15 pKa = 11.84SRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TRR21 pKa = 11.84WRR23 pKa = 11.84SSWIRR28 pKa = 11.84GMMCRR33 pKa = 11.84SRR35 pKa = 11.84SKK37 pKa = 10.23RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.26RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.63KK48 pKa = 8.89EE49 pKa = 3.67RR50 pKa = 11.84KK51 pKa = 8.78KK52 pKa = 9.51KK53 pKa = 8.8RR54 pKa = 11.84KK55 pKa = 8.98RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84TSRR61 pKa = 11.84AGRR64 pKa = 11.84RR65 pKa = 11.84VSRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84WRR72 pKa = 11.84QRR74 pKa = 2.75
Molecular weight: 9.63 kDa
Isoelectric point according different methods:
Peptides (in silico digests for buttom-up proteomics)
Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.| Try ESI |
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| ChTry ESI |
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| ArgC ESI |
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| LysN ESI |
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| TryLysC ESI |
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| Try MALDI |
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| ChTry MALDI |
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| ArgC MALDI |
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| LysN MALDI |
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| TryLysC MALDI |
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| Try LTQ |
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| ChTry LTQ |
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| ArgC LTQ |
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| LysN LTQ |
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| TryLysC LTQ |
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| Try MSlow |
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| ChTry MSlow |
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| ArgC MSlow |
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| LysN MSlow |
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| TryLysC MSlow |
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| Try MShigh |
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| ChTry MShigh |
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| ArgC MShigh |
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| LysN MShigh |
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| TryLysC MShigh |
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General Statistics
Number of major isoforms |
Number of additional isoforms |
Number of all proteins |
Number of amino acids |
Min. Seq. Length |
Max. Seq. Length |
Avg. Seq. Length |
Avg. Mol. Weight |
|---|---|---|---|---|---|---|---|
0 |
2100011 |
22 |
12565 |
360.8 |
40.71 |
Amino acid frequency
Ala |
Cys |
Asp |
Glu |
Phe |
Gly |
His |
Ile |
Lys |
Leu |
|---|---|---|---|---|---|---|---|---|---|
6.494 ± 0.036 | 2.129 ± 0.024 |
5.287 ± 0.025 | 7.366 ± 0.047 |
4.351 ± 0.021 | 5.697 ± 0.038 |
2.111 ± 0.016 | 5.826 ± 0.034 |
5.947 ± 0.047 | 9.257 ± 0.046 |
Met |
Asn |
Gln |
Pro |
Arg |
Ser |
Thr |
Val |
Trp |
Tyr |
|---|---|---|---|---|---|---|---|---|---|
2.65 ± 0.018 | 4.447 ± 0.025 |
4.505 ± 0.041 | 3.779 ± 0.026 |
5.558 ± 0.04 | 7.992 ± 0.045 |
5.156 ± 0.039 | 6.782 ± 0.044 |
1.084 ± 0.012 | 3.529 ± 0.025 |
Most of the basic statistics you can see at this page can be downloaded from this CSV file
See this proteome in:
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here
| Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 | Contact: Lukasz P. Kozlowski |