Amino acid dipepetide frequency for Mycobacterium phage Paito

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.226AlaAla: 20.226 ± 2.158
0.58AlaCys: 0.58 ± 0.163
7.612AlaAsp: 7.612 ± 1.011
8.264AlaGlu: 8.264 ± 1.021
3.842AlaPhe: 3.842 ± 0.62
13.267AlaGly: 13.267 ± 1.98
1.812AlaHis: 1.812 ± 0.409
6.96AlaIle: 6.96 ± 0.846
5.655AlaLys: 5.655 ± 0.797
10.222AlaLeu: 10.222 ± 0.835
3.19AlaMet: 3.19 ± 0.451
3.262AlaAsn: 3.262 ± 0.414
6.96AlaPro: 6.96 ± 0.927
5.292AlaGln: 5.292 ± 0.495
8.482AlaArg: 8.482 ± 1.095
5.945AlaSer: 5.945 ± 0.894
7.105AlaThr: 7.105 ± 0.663
8.337AlaVal: 8.337 ± 0.899
2.537AlaTrp: 2.537 ± 0.397
1.957AlaTyr: 1.957 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
0.942CysAla: 0.942 ± 0.33
0.0CysCys: 0.0 ± 0.0
0.29CysAsp: 0.29 ± 0.133
0.58CysGlu: 0.58 ± 0.203
0.217CysPhe: 0.217 ± 0.108
1.377CysGly: 1.377 ± 0.401
0.072CysHis: 0.072 ± 0.077
0.362CysIle: 0.362 ± 0.13
0.29CysLys: 0.29 ± 0.148
0.29CysLeu: 0.29 ± 0.156
0.145CysMet: 0.145 ± 0.095
0.29CysAsn: 0.29 ± 0.141
0.652CysPro: 0.652 ± 0.314
0.58CysGln: 0.58 ± 0.228
1.087CysArg: 1.087 ± 0.267
0.652CysSer: 0.652 ± 0.239
0.362CysThr: 0.362 ± 0.204
0.29CysVal: 0.29 ± 0.171
0.145CysTrp: 0.145 ± 0.097
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.989AspAla: 8.989 ± 0.753
0.435AspCys: 0.435 ± 0.24
6.235AspAsp: 6.235 ± 0.877
5.002AspGlu: 5.002 ± 0.635
2.537AspPhe: 2.537 ± 0.39
7.974AspGly: 7.974 ± 0.717
1.015AspHis: 1.015 ± 0.259
2.175AspIle: 2.175 ± 0.426
1.885AspLys: 1.885 ± 0.505
4.712AspLeu: 4.712 ± 0.573
0.87AspMet: 0.87 ± 0.214
1.377AspAsn: 1.377 ± 0.35
4.785AspPro: 4.785 ± 0.702
2.827AspGln: 2.827 ± 0.524
4.567AspArg: 4.567 ± 0.67
2.9AspSer: 2.9 ± 0.418
3.625AspThr: 3.625 ± 0.432
4.64AspVal: 4.64 ± 0.563
1.16AspTrp: 1.16 ± 0.288
2.102AspTyr: 2.102 ± 0.507
0.0AspXaa: 0.0 ± 0.0
Glu
6.525GluAla: 6.525 ± 0.725
0.362GluCys: 0.362 ± 0.194
4.712GluAsp: 4.712 ± 0.679
3.335GluGlu: 3.335 ± 0.528
1.812GluPhe: 1.812 ± 0.431
3.625GluGly: 3.625 ± 0.539
2.32GluHis: 2.32 ± 0.469
2.247GluIle: 2.247 ± 0.396
1.305GluLys: 1.305 ± 0.302
5.872GluLeu: 5.872 ± 0.787
1.45GluMet: 1.45 ± 0.328
2.102GluAsn: 2.102 ± 0.518
3.842GluPro: 3.842 ± 0.753
2.465GluGln: 2.465 ± 0.481
3.987GluArg: 3.987 ± 0.821
2.392GluSer: 2.392 ± 0.39
2.9GluThr: 2.9 ± 0.513
4.06GluVal: 4.06 ± 0.565
1.015GluTrp: 1.015 ± 0.27
0.652GluTyr: 0.652 ± 0.203
0.0GluXaa: 0.0 ± 0.0
Phe
3.262PheAla: 3.262 ± 0.352
0.725PheCys: 0.725 ± 0.242
2.9PheAsp: 2.9 ± 0.42
2.537PheGlu: 2.537 ± 0.49
0.725PhePhe: 0.725 ± 0.227
3.262PheGly: 3.262 ± 0.688
0.435PheHis: 0.435 ± 0.184
0.725PheIle: 0.725 ± 0.249
0.942PheLys: 0.942 ± 0.218
2.247PheLeu: 2.247 ± 0.435
0.435PheMet: 0.435 ± 0.153
1.015PheAsn: 1.015 ± 0.279
1.305PhePro: 1.305 ± 0.35
0.58PheGln: 0.58 ± 0.203
1.812PheArg: 1.812 ± 0.356
1.595PheSer: 1.595 ± 0.325
1.595PheThr: 1.595 ± 0.251
2.102PheVal: 2.102 ± 0.397
0.362PheTrp: 0.362 ± 0.155
0.507PheTyr: 0.507 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
10.512GlyAla: 10.512 ± 1.142
0.87GlyCys: 0.87 ± 0.206
6.09GlyAsp: 6.09 ± 0.69
5.147GlyGlu: 5.147 ± 0.457
2.972GlyPhe: 2.972 ± 0.424
7.612GlyGly: 7.612 ± 1.48
2.175GlyHis: 2.175 ± 0.449
4.422GlyIle: 4.422 ± 0.61
4.93GlyLys: 4.93 ± 0.745
6.742GlyLeu: 6.742 ± 0.895
1.885GlyMet: 1.885 ± 0.336
3.77GlyAsn: 3.77 ± 0.702
4.06GlyPro: 4.06 ± 0.707
2.9GlyGln: 2.9 ± 0.507
5.8GlyArg: 5.8 ± 0.691
5.437GlySer: 5.437 ± 0.704
5.002GlyThr: 5.002 ± 0.792
5.437GlyVal: 5.437 ± 0.863
1.812GlyTrp: 1.812 ± 0.34
2.392GlyTyr: 2.392 ± 0.505
0.0GlyXaa: 0.0 ± 0.0
His
2.537HisAla: 2.537 ± 0.529
0.29HisCys: 0.29 ± 0.142
1.305HisAsp: 1.305 ± 0.342
0.652HisGlu: 0.652 ± 0.221
0.435HisPhe: 0.435 ± 0.192
2.03HisGly: 2.03 ± 0.439
0.652HisHis: 0.652 ± 0.242
1.16HisIle: 1.16 ± 0.256
0.87HisLys: 0.87 ± 0.251
1.45HisLeu: 1.45 ± 0.381
0.58HisMet: 0.58 ± 0.191
0.725HisAsn: 0.725 ± 0.244
1.667HisPro: 1.667 ± 0.46
0.58HisGln: 0.58 ± 0.213
2.102HisArg: 2.102 ± 0.429
0.87HisSer: 0.87 ± 0.22
1.232HisThr: 1.232 ± 0.365
1.232HisVal: 1.232 ± 0.273
0.725HisTrp: 0.725 ± 0.26
0.507HisTyr: 0.507 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
6.67IleAla: 6.67 ± 0.741
0.362IleCys: 0.362 ± 0.153
3.697IleAsp: 3.697 ± 0.482
3.625IleGlu: 3.625 ± 0.599
1.015IlePhe: 1.015 ± 0.203
3.842IleGly: 3.842 ± 1.026
0.725IleHis: 0.725 ± 0.19
1.232IleIle: 1.232 ± 0.312
1.377IleLys: 1.377 ± 0.409
2.61IleLeu: 2.61 ± 0.507
0.507IleMet: 0.507 ± 0.226
1.015IleAsn: 1.015 ± 0.277
3.842IlePro: 3.842 ± 0.712
1.377IleGln: 1.377 ± 0.292
4.132IleArg: 4.132 ± 0.561
2.102IleSer: 2.102 ± 0.515
2.9IleThr: 2.9 ± 0.506
3.407IleVal: 3.407 ± 0.688
0.942IleTrp: 0.942 ± 0.255
0.87IleTyr: 0.87 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
5.655LysAla: 5.655 ± 0.759
0.362LysCys: 0.362 ± 0.136
1.595LysAsp: 1.595 ± 0.417
0.652LysGlu: 0.652 ± 0.247
1.087LysPhe: 1.087 ± 0.246
1.957LysGly: 1.957 ± 0.351
0.725LysHis: 0.725 ± 0.267
2.247LysIle: 2.247 ± 0.359
1.522LysLys: 1.522 ± 0.34
2.537LysLeu: 2.537 ± 0.392
0.942LysMet: 0.942 ± 0.232
1.45LysAsn: 1.45 ± 0.329
3.045LysPro: 3.045 ± 0.422
1.522LysGln: 1.522 ± 0.402
2.9LysArg: 2.9 ± 0.525
1.885LysSer: 1.885 ± 0.307
3.19LysThr: 3.19 ± 0.42
1.812LysVal: 1.812 ± 0.323
0.435LysTrp: 0.435 ± 0.172
0.87LysTyr: 0.87 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
8.554LeuAla: 8.554 ± 0.803
0.435LeuCys: 0.435 ± 0.209
4.857LeuAsp: 4.857 ± 0.49
4.277LeuGlu: 4.277 ± 0.717
2.102LeuPhe: 2.102 ± 0.416
7.105LeuGly: 7.105 ± 0.98
1.667LeuHis: 1.667 ± 0.354
4.205LeuIle: 4.205 ± 0.54
2.755LeuLys: 2.755 ± 0.488
4.64LeuLeu: 4.64 ± 0.705
1.885LeuMet: 1.885 ± 0.322
1.885LeuAsn: 1.885 ± 0.327
4.422LeuPro: 4.422 ± 0.577
1.74LeuGln: 1.74 ± 0.362
6.162LeuArg: 6.162 ± 0.742
4.277LeuSer: 4.277 ± 0.456
5.22LeuThr: 5.22 ± 0.843
4.64LeuVal: 4.64 ± 0.672
1.087LeuTrp: 1.087 ± 0.544
1.522LeuTyr: 1.522 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.175MetAla: 2.175 ± 0.367
0.145MetCys: 0.145 ± 0.091
1.087MetAsp: 1.087 ± 0.331
0.725MetGlu: 0.725 ± 0.182
0.652MetPhe: 0.652 ± 0.187
1.885MetGly: 1.885 ± 0.353
0.87MetHis: 0.87 ± 0.366
1.16MetIle: 1.16 ± 0.25
0.58MetLys: 0.58 ± 0.213
2.392MetLeu: 2.392 ± 0.524
0.652MetMet: 0.652 ± 0.225
0.652MetAsn: 0.652 ± 0.199
0.797MetPro: 0.797 ± 0.296
0.435MetGln: 0.435 ± 0.187
1.885MetArg: 1.885 ± 0.357
2.175MetSer: 2.175 ± 0.338
2.465MetThr: 2.465 ± 0.379
1.45MetVal: 1.45 ± 0.294
0.362MetTrp: 0.362 ± 0.151
0.217MetTyr: 0.217 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
4.132AsnAla: 4.132 ± 0.696
0.362AsnCys: 0.362 ± 0.158
1.812AsnAsp: 1.812 ± 0.465
1.595AsnGlu: 1.595 ± 0.326
0.797AsnPhe: 0.797 ± 0.256
3.045AsnGly: 3.045 ± 0.445
0.652AsnHis: 0.652 ± 0.214
1.377AsnIle: 1.377 ± 0.315
0.725AsnLys: 0.725 ± 0.268
1.74AsnLeu: 1.74 ± 0.318
0.58AsnMet: 0.58 ± 0.235
0.652AsnAsn: 0.652 ± 0.218
3.552AsnPro: 3.552 ± 0.458
1.377AsnGln: 1.377 ± 0.297
2.102AsnArg: 2.102 ± 0.309
0.507AsnSer: 0.507 ± 0.168
1.812AsnThr: 1.812 ± 0.332
1.45AsnVal: 1.45 ± 0.42
0.58AsnTrp: 0.58 ± 0.158
0.652AsnTyr: 0.652 ± 0.181
0.0AsnXaa: 0.0 ± 0.0
Pro
9.932ProAla: 9.932 ± 0.986
0.507ProCys: 0.507 ± 0.257
5.655ProAsp: 5.655 ± 0.91
4.422ProGlu: 4.422 ± 0.636
1.232ProPhe: 1.232 ± 0.315
5.437ProGly: 5.437 ± 0.94
1.377ProHis: 1.377 ± 0.275
2.465ProIle: 2.465 ± 0.399
1.957ProLys: 1.957 ± 0.435
3.77ProLeu: 3.77 ± 0.436
0.942ProMet: 0.942 ± 0.33
1.74ProAsn: 1.74 ± 0.408
3.552ProPro: 3.552 ± 0.576
2.175ProGln: 2.175 ± 0.468
3.262ProArg: 3.262 ± 0.61
3.335ProSer: 3.335 ± 0.426
4.205ProThr: 4.205 ± 0.596
3.552ProVal: 3.552 ± 0.437
0.87ProTrp: 0.87 ± 0.291
0.797ProTyr: 0.797 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
4.712GlnAla: 4.712 ± 0.725
0.145GlnCys: 0.145 ± 0.114
2.247GlnAsp: 2.247 ± 0.379
0.87GlnGlu: 0.87 ± 0.21
1.305GlnPhe: 1.305 ± 0.278
2.465GlnGly: 2.465 ± 0.436
1.377GlnHis: 1.377 ± 0.403
1.885GlnIle: 1.885 ± 0.388
0.942GlnLys: 0.942 ± 0.24
3.407GlnLeu: 3.407 ± 0.483
0.942GlnMet: 0.942 ± 0.267
1.232GlnAsn: 1.232 ± 0.388
1.957GlnPro: 1.957 ± 0.329
2.175GlnGln: 2.175 ± 0.428
2.682GlnArg: 2.682 ± 0.415
2.102GlnSer: 2.102 ± 0.42
1.667GlnThr: 1.667 ± 0.291
2.03GlnVal: 2.03 ± 0.369
0.652GlnTrp: 0.652 ± 0.204
0.507GlnTyr: 0.507 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
9.424ArgAla: 9.424 ± 1.056
0.942ArgCys: 0.942 ± 0.335
5.002ArgAsp: 5.002 ± 0.494
3.262ArgGlu: 3.262 ± 0.405
1.522ArgPhe: 1.522 ± 0.326
5.437ArgGly: 5.437 ± 0.716
1.232ArgHis: 1.232 ± 0.301
3.915ArgIle: 3.915 ± 0.455
2.827ArgLys: 2.827 ± 0.493
5.002ArgLeu: 5.002 ± 0.739
1.812ArgMet: 1.812 ± 0.311
2.32ArgAsn: 2.32 ± 0.388
4.567ArgPro: 4.567 ± 0.518
2.972ArgGln: 2.972 ± 0.492
7.54ArgArg: 7.54 ± 1.204
2.61ArgSer: 2.61 ± 0.541
3.842ArgThr: 3.842 ± 0.38
4.35ArgVal: 4.35 ± 0.767
1.522ArgTrp: 1.522 ± 0.387
1.522ArgTyr: 1.522 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
6.597SerAla: 6.597 ± 1.189
0.725SerCys: 0.725 ± 0.292
3.697SerAsp: 3.697 ± 0.629
2.827SerGlu: 2.827 ± 0.442
1.522SerPhe: 1.522 ± 0.283
4.785SerGly: 4.785 ± 0.891
0.652SerHis: 0.652 ± 0.216
2.537SerIle: 2.537 ± 0.448
2.61SerLys: 2.61 ± 0.428
3.262SerLeu: 3.262 ± 0.547
1.667SerMet: 1.667 ± 0.313
1.522SerAsn: 1.522 ± 0.41
2.682SerPro: 2.682 ± 0.537
0.942SerGln: 0.942 ± 0.256
3.117SerArg: 3.117 ± 0.411
3.262SerSer: 3.262 ± 0.647
3.262SerThr: 3.262 ± 0.444
3.335SerVal: 3.335 ± 0.58
1.16SerTrp: 1.16 ± 0.331
1.16SerTyr: 1.16 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
9.134ThrAla: 9.134 ± 0.867
0.29ThrCys: 0.29 ± 0.132
3.915ThrAsp: 3.915 ± 0.501
3.48ThrGlu: 3.48 ± 0.413
2.392ThrPhe: 2.392 ± 0.329
5.365ThrGly: 5.365 ± 0.804
1.377ThrHis: 1.377 ± 0.307
2.9ThrIle: 2.9 ± 0.563
1.595ThrLys: 1.595 ± 0.333
3.552ThrLeu: 3.552 ± 0.443
2.03ThrMet: 2.03 ± 0.364
1.667ThrAsn: 1.667 ± 0.298
4.132ThrPro: 4.132 ± 0.597
1.16ThrGln: 1.16 ± 0.223
3.625ThrArg: 3.625 ± 0.481
3.842ThrSer: 3.842 ± 0.55
3.915ThrThr: 3.915 ± 0.54
5.002ThrVal: 5.002 ± 0.622
0.87ThrTrp: 0.87 ± 0.239
1.015ThrTyr: 1.015 ± 0.311
0.0ThrXaa: 0.0 ± 0.0
Val
7.322ValAla: 7.322 ± 0.707
0.797ValCys: 0.797 ± 0.284
5.075ValAsp: 5.075 ± 0.582
4.495ValGlu: 4.495 ± 0.567
2.03ValPhe: 2.03 ± 0.365
5.582ValGly: 5.582 ± 0.575
1.305ValHis: 1.305 ± 0.286
2.465ValIle: 2.465 ± 0.605
2.247ValLys: 2.247 ± 0.444
4.857ValLeu: 4.857 ± 0.535
1.667ValMet: 1.667 ± 0.443
2.03ValAsn: 2.03 ± 0.354
3.335ValPro: 3.335 ± 0.533
2.102ValGln: 2.102 ± 0.403
3.842ValArg: 3.842 ± 0.678
2.9ValSer: 2.9 ± 0.493
5.002ValThr: 5.002 ± 0.703
5.365ValVal: 5.365 ± 0.697
1.522ValTrp: 1.522 ± 0.38
1.74ValTyr: 1.74 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
2.392TrpAla: 2.392 ± 0.475
0.217TrpCys: 0.217 ± 0.135
1.16TrpAsp: 1.16 ± 0.236
0.362TrpGlu: 0.362 ± 0.166
0.435TrpPhe: 0.435 ± 0.185
1.595TrpGly: 1.595 ± 0.311
0.507TrpHis: 0.507 ± 0.187
1.087TrpIle: 1.087 ± 0.238
0.58TrpLys: 0.58 ± 0.207
2.102TrpLeu: 2.102 ± 0.592
0.362TrpMet: 0.362 ± 0.195
0.507TrpAsn: 0.507 ± 0.206
1.16TrpPro: 1.16 ± 0.312
1.087TrpGln: 1.087 ± 0.225
1.232TrpArg: 1.232 ± 0.25
1.232TrpSer: 1.232 ± 0.277
0.87TrpThr: 0.87 ± 0.234
0.725TrpVal: 0.725 ± 0.173
0.072TrpTrp: 0.072 ± 0.071
0.507TrpTyr: 0.507 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.812TyrAla: 1.812 ± 0.386
0.072TyrCys: 0.072 ± 0.075
0.87TyrAsp: 0.87 ± 0.261
0.87TyrGlu: 0.87 ± 0.192
0.435TyrPhe: 0.435 ± 0.2
2.32TyrGly: 2.32 ± 0.379
0.652TyrHis: 0.652 ± 0.201
0.725TyrIle: 0.725 ± 0.178
0.797TyrLys: 0.797 ± 0.22
2.03TyrLeu: 2.03 ± 0.399
0.145TyrMet: 0.145 ± 0.101
0.29TyrAsn: 0.29 ± 0.135
0.87TyrPro: 0.87 ± 0.259
1.015TyrGln: 1.015 ± 0.264
1.305TyrArg: 1.305 ± 0.284
1.305TyrSer: 1.305 ± 0.3
1.087TyrThr: 1.087 ± 0.235
2.465TyrVal: 2.465 ± 0.444
0.435TyrTrp: 0.435 ± 0.141
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13795 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski