Amino acid dipepetide frequency for Mycobacterium sp. ACS4331

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.959AlaAla: 19.959 ± 0.164
0.985AlaCys: 0.985 ± 0.026
8.792AlaAsp: 8.792 ± 0.066
8.152AlaGlu: 8.152 ± 0.084
3.493AlaPhe: 3.493 ± 0.045
11.839AlaGly: 11.839 ± 0.127
2.646AlaHis: 2.646 ± 0.043
5.106AlaIle: 5.106 ± 0.054
2.963AlaLys: 2.963 ± 0.057
13.199AlaLeu: 13.199 ± 0.102
2.791AlaMet: 2.791 ± 0.036
2.498AlaAsn: 2.498 ± 0.04
6.564AlaPro: 6.564 ± 0.083
4.154AlaGln: 4.154 ± 0.047
8.392AlaArg: 8.392 ± 0.085
5.915AlaSer: 5.915 ± 0.059
7.156AlaThr: 7.156 ± 0.067
12.132AlaVal: 12.132 ± 0.093
1.589AlaTrp: 1.589 ± 0.033
2.275AlaTyr: 2.275 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.092CysAla: 1.092 ± 0.026
0.076CysCys: 0.076 ± 0.007
0.502CysAsp: 0.502 ± 0.017
0.398CysGlu: 0.398 ± 0.016
0.224CysPhe: 0.224 ± 0.013
0.942CysGly: 0.942 ± 0.03
0.194CysHis: 0.194 ± 0.009
0.254CysIle: 0.254 ± 0.013
0.095CysLys: 0.095 ± 0.008
0.659CysLeu: 0.659 ± 0.019
0.129CysMet: 0.129 ± 0.008
0.163CysAsn: 0.163 ± 0.01
0.485CysPro: 0.485 ± 0.019
0.225CysGln: 0.225 ± 0.011
0.588CysArg: 0.588 ± 0.02
0.444CysSer: 0.444 ± 0.014
0.509CysThr: 0.509 ± 0.018
0.705CysVal: 0.705 ± 0.019
0.135CysTrp: 0.135 ± 0.009
0.18CysTyr: 0.18 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.88AspAla: 7.88 ± 0.072
0.479AspCys: 0.479 ± 0.017
4.393AspAsp: 4.393 ± 0.063
4.167AspGlu: 4.167 ± 0.059
1.835AspPhe: 1.835 ± 0.031
6.123AspGly: 6.123 ± 0.079
1.455AspHis: 1.455 ± 0.031
2.677AspIle: 2.677 ± 0.042
1.219AspLys: 1.219 ± 0.034
6.388AspLeu: 6.388 ± 0.058
1.06AspMet: 1.06 ± 0.028
1.238AspAsn: 1.238 ± 0.027
4.593AspPro: 4.593 ± 0.048
1.739AspGln: 1.739 ± 0.034
4.603AspArg: 4.603 ± 0.049
2.94AspSer: 2.94 ± 0.051
3.338AspThr: 3.338 ± 0.044
5.561AspVal: 5.561 ± 0.06
0.997AspTrp: 0.997 ± 0.024
1.364AspTyr: 1.364 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.461GluAla: 6.461 ± 0.071
0.376GluCys: 0.376 ± 0.014
2.711GluAsp: 2.711 ± 0.044
2.581GluGlu: 2.581 ± 0.045
1.968GluPhe: 1.968 ± 0.036
3.479GluGly: 3.479 ± 0.05
1.63GluHis: 1.63 ± 0.03
2.654GluIle: 2.654 ± 0.042
1.339GluLys: 1.339 ± 0.034
6.36GluLeu: 6.36 ± 0.073
1.101GluMet: 1.101 ± 0.026
1.163GluAsn: 1.163 ± 0.027
3.019GluPro: 3.019 ± 0.048
2.248GluGln: 2.248 ± 0.04
4.372GluArg: 4.372 ± 0.058
2.863GluSer: 2.863 ± 0.039
2.739GluThr: 2.739 ± 0.041
4.658GluVal: 4.658 ± 0.053
0.764GluTrp: 0.764 ± 0.024
1.151GluTyr: 1.151 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.978PheAla: 3.978 ± 0.052
0.33PheCys: 0.33 ± 0.013
2.521PheAsp: 2.521 ± 0.041
1.645PheGlu: 1.645 ± 0.034
1.001PhePhe: 1.001 ± 0.024
3.557PheGly: 3.557 ± 0.047
0.644PheHis: 0.644 ± 0.022
1.165PheIle: 1.165 ± 0.032
0.548PheLys: 0.548 ± 0.019
2.581PheLeu: 2.581 ± 0.048
0.524PheMet: 0.524 ± 0.016
0.806PheAsn: 0.806 ± 0.024
1.444PhePro: 1.444 ± 0.032
0.666PheGln: 0.666 ± 0.021
1.677PheArg: 1.677 ± 0.033
1.738PheSer: 1.738 ± 0.028
2.178PheThr: 2.178 ± 0.037
2.608PheVal: 2.608 ± 0.042
0.45PheTrp: 0.45 ± 0.018
0.693PheTyr: 0.693 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
10.593GlyAla: 10.593 ± 0.124
0.757GlyCys: 0.757 ± 0.023
5.221GlyAsp: 5.221 ± 0.071
4.381GlyGlu: 4.381 ± 0.052
3.052GlyPhe: 3.052 ± 0.044
8.222GlyGly: 8.222 ± 0.212
2.041GlyHis: 2.041 ± 0.037
3.941GlyIle: 3.941 ± 0.045
1.896GlyLys: 1.896 ± 0.038
8.65GlyLeu: 8.65 ± 0.072
2.101GlyMet: 2.101 ± 0.033
1.944GlyAsn: 1.944 ± 0.093
4.634GlyPro: 4.634 ± 0.053
2.93GlyGln: 2.93 ± 0.042
6.328GlyArg: 6.328 ± 0.063
5.127GlySer: 5.127 ± 0.067
5.471GlyThr: 5.471 ± 0.077
8.195GlyVal: 8.195 ± 0.08
1.622GlyTrp: 1.622 ± 0.037
2.315GlyTyr: 2.315 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.428HisAla: 2.428 ± 0.038
0.225HisCys: 0.225 ± 0.01
1.315HisAsp: 1.315 ± 0.029
1.044HisGlu: 1.044 ± 0.026
0.715HisPhe: 0.715 ± 0.019
2.098HisGly: 2.098 ± 0.036
0.679HisHis: 0.679 ± 0.024
0.866HisIle: 0.866 ± 0.025
0.31HisLys: 0.31 ± 0.014
2.318HisLeu: 2.318 ± 0.036
0.376HisMet: 0.376 ± 0.013
0.476HisAsn: 0.476 ± 0.017
1.676HisPro: 1.676 ± 0.033
0.645HisGln: 0.645 ± 0.021
1.933HisArg: 1.933 ± 0.038
1.098HisSer: 1.098 ± 0.025
1.208HisThr: 1.208 ± 0.027
1.729HisVal: 1.729 ± 0.028
0.363HisTrp: 0.363 ± 0.015
0.512HisTyr: 0.512 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.382IleAla: 6.382 ± 0.061
0.39IleCys: 0.39 ± 0.017
3.367IleAsp: 3.367 ± 0.048
2.679IleGlu: 2.679 ± 0.043
1.042IlePhe: 1.042 ± 0.028
4.321IleGly: 4.321 ± 0.049
0.754IleHis: 0.754 ± 0.022
1.484IleIle: 1.484 ± 0.035
0.867IleLys: 0.867 ± 0.024
3.071IleLeu: 3.071 ± 0.039
0.611IleMet: 0.611 ± 0.019
1.141IleAsn: 1.141 ± 0.027
2.437IlePro: 2.437 ± 0.038
0.902IleGln: 0.902 ± 0.022
2.634IleArg: 2.634 ± 0.035
2.326IleSer: 2.326 ± 0.037
2.825IleThr: 2.825 ± 0.045
3.608IleVal: 3.608 ± 0.051
0.471IleTrp: 0.471 ± 0.015
0.804IleTyr: 0.804 ± 0.021
0.0IleXaa: 0.0 ± 0.0
Lys
2.591LysAla: 2.591 ± 0.056
0.108LysCys: 0.108 ± 0.008
1.089LysAsp: 1.089 ± 0.028
0.919LysGlu: 0.919 ± 0.025
0.626LysPhe: 0.626 ± 0.023
1.488LysGly: 1.488 ± 0.033
0.461LysHis: 0.461 ± 0.018
0.926LysIle: 0.926 ± 0.024
0.621LysLys: 0.621 ± 0.026
2.054LysLeu: 2.054 ± 0.037
0.46LysMet: 0.46 ± 0.017
0.501LysAsn: 0.501 ± 0.018
1.365LysPro: 1.365 ± 0.03
0.661LysGln: 0.661 ± 0.02
1.505LysArg: 1.505 ± 0.029
1.165LysSer: 1.165 ± 0.028
1.219LysThr: 1.219 ± 0.03
1.844LysVal: 1.844 ± 0.037
0.302LysTrp: 0.302 ± 0.014
0.454LysTyr: 0.454 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
13.789LeuAla: 13.789 ± 0.092
0.799LeuCys: 0.799 ± 0.025
6.568LeuAsp: 6.568 ± 0.068
4.543LeuGlu: 4.543 ± 0.059
2.689LeuPhe: 2.689 ± 0.047
8.786LeuGly: 8.786 ± 0.076
2.016LeuHis: 2.016 ± 0.035
4.171LeuIle: 4.171 ± 0.055
1.901LeuLys: 1.901 ± 0.041
9.648LeuLeu: 9.648 ± 0.091
1.798LeuMet: 1.798 ± 0.036
2.181LeuAsn: 2.181 ± 0.033
5.862LeuPro: 5.862 ± 0.066
2.543LeuGln: 2.543 ± 0.039
7.489LeuArg: 7.489 ± 0.071
5.8LeuSer: 5.8 ± 0.053
6.557LeuThr: 6.557 ± 0.068
8.426LeuVal: 8.426 ± 0.073
1.187LeuTrp: 1.187 ± 0.027
1.623LeuTyr: 1.623 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.618MetAla: 2.618 ± 0.039
0.194MetCys: 0.194 ± 0.01
0.869MetAsp: 0.869 ± 0.021
0.721MetGlu: 0.721 ± 0.02
0.679MetPhe: 0.679 ± 0.021
1.522MetGly: 1.522 ± 0.032
0.406MetHis: 0.406 ± 0.016
0.916MetIle: 0.916 ± 0.025
0.455MetLys: 0.455 ± 0.017
2.041MetLeu: 2.041 ± 0.039
0.441MetMet: 0.441 ± 0.016
0.476MetAsn: 0.476 ± 0.017
1.205MetPro: 1.205 ± 0.025
0.545MetGln: 0.545 ± 0.019
1.514MetArg: 1.514 ± 0.03
1.562MetSer: 1.562 ± 0.033
1.847MetThr: 1.847 ± 0.034
1.696MetVal: 1.696 ± 0.031
0.264MetTrp: 0.264 ± 0.012
0.326MetTyr: 0.326 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.672AsnAla: 2.672 ± 0.047
0.175AsnCys: 0.175 ± 0.01
1.223AsnAsp: 1.223 ± 0.032
0.993AsnGlu: 0.993 ± 0.026
0.701AsnPhe: 0.701 ± 0.022
2.107AsnGly: 2.107 ± 0.055
0.448AsnHis: 0.448 ± 0.014
0.946AsnIle: 0.946 ± 0.03
0.402AsnLys: 0.402 ± 0.017
2.147AsnLeu: 2.147 ± 0.042
0.437AsnMet: 0.437 ± 0.016
0.559AsnAsn: 0.559 ± 0.022
1.828AsnPro: 1.828 ± 0.036
0.641AsnGln: 0.641 ± 0.024
1.582AsnArg: 1.582 ± 0.028
1.193AsnSer: 1.193 ± 0.04
1.333AsnThr: 1.333 ± 0.042
1.759AsnVal: 1.759 ± 0.034
0.394AsnTrp: 0.394 ± 0.015
0.527AsnTyr: 0.527 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.348ProAla: 7.348 ± 0.08
0.273ProCys: 0.273 ± 0.013
4.617ProAsp: 4.617 ± 0.056
3.93ProGlu: 3.93 ± 0.052
1.668ProPhe: 1.668 ± 0.031
5.656ProGly: 5.656 ± 0.064
1.228ProHis: 1.228 ± 0.027
2.16ProIle: 2.16 ± 0.034
1.298ProLys: 1.298 ± 0.029
4.943ProLeu: 4.943 ± 0.064
1.216ProMet: 1.216 ± 0.027
1.322ProAsn: 1.322 ± 0.026
3.536ProPro: 3.536 ± 0.077
1.743ProGln: 1.743 ± 0.035
3.398ProArg: 3.398 ± 0.042
3.094ProSer: 3.094 ± 0.041
3.503ProThr: 3.503 ± 0.048
5.193ProVal: 5.193 ± 0.059
0.836ProTrp: 0.836 ± 0.021
1.184ProTyr: 1.184 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.533GlnAla: 3.533 ± 0.045
0.224GlnCys: 0.224 ± 0.013
1.329GlnAsp: 1.329 ± 0.03
1.165GlnGlu: 1.165 ± 0.027
0.985GlnPhe: 0.985 ± 0.023
2.084GlnGly: 2.084 ± 0.043
0.716GlnHis: 0.716 ± 0.019
1.526GlnIle: 1.526 ± 0.034
0.629GlnLys: 0.629 ± 0.018
3.296GlnLeu: 3.296 ± 0.048
0.72GlnMet: 0.72 ± 0.02
0.667GlnAsn: 0.667 ± 0.024
1.684GlnPro: 1.684 ± 0.034
1.239GlnGln: 1.239 ± 0.033
2.802GlnArg: 2.802 ± 0.045
1.43GlnSer: 1.43 ± 0.031
1.605GlnThr: 1.605 ± 0.032
2.499GlnVal: 2.499 ± 0.046
0.604GlnTrp: 0.604 ± 0.018
0.675GlnTyr: 0.675 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.336ArgAla: 8.336 ± 0.078
0.603ArgCys: 0.603 ± 0.019
4.393ArgAsp: 4.393 ± 0.048
3.77ArgGlu: 3.77 ± 0.045
2.446ArgPhe: 2.446 ± 0.038
5.209ArgGly: 5.209 ± 0.056
1.755ArgHis: 1.755 ± 0.032
3.576ArgIle: 3.576 ± 0.046
1.301ArgLys: 1.301 ± 0.032
7.271ArgLeu: 7.271 ± 0.075
1.788ArgMet: 1.788 ± 0.033
1.61ArgAsn: 1.61 ± 0.032
3.867ArgPro: 3.867 ± 0.056
2.211ArgGln: 2.211 ± 0.037
6.533ArgArg: 6.533 ± 0.068
3.937ArgSer: 3.937 ± 0.046
4.298ArgThr: 4.298 ± 0.052
5.756ArgVal: 5.756 ± 0.064
1.321ArgTrp: 1.321 ± 0.028
1.792ArgTyr: 1.792 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
7.16SerAla: 7.16 ± 0.072
0.416SerCys: 0.416 ± 0.016
3.266SerAsp: 3.266 ± 0.046
2.723SerGlu: 2.723 ± 0.043
1.718SerPhe: 1.718 ± 0.031
5.742SerGly: 5.742 ± 0.085
1.05SerHis: 1.05 ± 0.023
2.117SerIle: 2.117 ± 0.037
0.975SerLys: 0.975 ± 0.023
4.768SerLeu: 4.768 ± 0.051
1.275SerMet: 1.275 ± 0.027
1.091SerAsn: 1.091 ± 0.023
3.036SerPro: 3.036 ± 0.044
1.436SerGln: 1.436 ± 0.033
3.716SerArg: 3.716 ± 0.057
3.013SerSer: 3.013 ± 0.043
3.452SerThr: 3.452 ± 0.04
4.695SerVal: 4.695 ± 0.053
0.885SerTrp: 0.885 ± 0.019
1.157SerTyr: 1.157 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
8.133ThrAla: 8.133 ± 0.073
0.417ThrCys: 0.417 ± 0.016
3.8ThrAsp: 3.8 ± 0.053
3.323ThrGlu: 3.323 ± 0.048
1.881ThrPhe: 1.881 ± 0.034
5.988ThrGly: 5.988 ± 0.077
1.197ThrHis: 1.197 ± 0.027
2.323ThrIle: 2.323 ± 0.036
1.162ThrLys: 1.162 ± 0.03
5.815ThrLeu: 5.815 ± 0.056
1.201ThrMet: 1.201 ± 0.026
1.209ThrAsn: 1.209 ± 0.031
4.01ThrPro: 4.01 ± 0.048
1.486ThrGln: 1.486 ± 0.03
3.692ThrArg: 3.692 ± 0.046
3.202ThrSer: 3.202 ± 0.047
3.879ThrThr: 3.879 ± 0.065
6.125ThrVal: 6.125 ± 0.064
0.904ThrTrp: 0.904 ± 0.024
1.271ThrTyr: 1.271 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
11.7ValAla: 11.7 ± 0.104
0.779ValCys: 0.779 ± 0.022
5.879ValAsp: 5.879 ± 0.055
4.776ValGlu: 4.776 ± 0.051
2.741ValPhe: 2.741 ± 0.041
7.232ValGly: 7.232 ± 0.074
1.897ValHis: 1.897 ± 0.033
4.008ValIle: 4.008 ± 0.045
1.719ValLys: 1.719 ± 0.032
9.301ValLeu: 9.301 ± 0.076
1.637ValMet: 1.637 ± 0.03
2.114ValAsn: 2.114 ± 0.039
4.861ValPro: 4.861 ± 0.063
2.161ValGln: 2.161 ± 0.037
6.0ValArg: 6.0 ± 0.063
4.763ValSer: 4.763 ± 0.054
5.753ValThr: 5.753 ± 0.059
8.828ValVal: 8.828 ± 0.082
1.067ValTrp: 1.067 ± 0.026
1.565ValTyr: 1.565 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.552TrpAla: 1.552 ± 0.033
0.16TrpCys: 0.16 ± 0.011
0.809TrpAsp: 0.809 ± 0.019
0.669TrpGlu: 0.669 ± 0.019
0.539TrpPhe: 0.539 ± 0.018
1.002TrpGly: 1.002 ± 0.028
0.357TrpHis: 0.357 ± 0.016
0.637TrpIle: 0.637 ± 0.017
0.303TrpLys: 0.303 ± 0.014
1.688TrpLeu: 1.688 ± 0.034
0.335TrpMet: 0.335 ± 0.016
0.416TrpAsn: 0.416 ± 0.015
0.856TrpPro: 0.856 ± 0.024
0.598TrpGln: 0.598 ± 0.019
1.243TrpArg: 1.243 ± 0.029
0.921TrpSer: 0.921 ± 0.023
0.939TrpThr: 0.939 ± 0.022
1.146TrpVal: 1.146 ± 0.027
0.339TrpTrp: 0.339 ± 0.015
0.315TrpTyr: 0.315 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.393TyrAla: 2.393 ± 0.042
0.233TyrCys: 0.233 ± 0.01
1.387TyrAsp: 1.387 ± 0.032
1.062TyrGlu: 1.062 ± 0.024
0.748TyrPhe: 0.748 ± 0.023
2.024TyrGly: 2.024 ± 0.04
0.441TyrHis: 0.441 ± 0.017
0.661TyrIle: 0.661 ± 0.018
0.354TyrLys: 0.354 ± 0.015
2.181TyrLeu: 2.181 ± 0.037
0.292TyrMet: 0.292 ± 0.013
0.49TyrAsn: 0.49 ± 0.021
1.184TyrPro: 1.184 ± 0.028
0.638TyrGln: 0.638 ± 0.018
1.771TyrArg: 1.771 ± 0.031
1.161TyrSer: 1.161 ± 0.024
1.208TyrThr: 1.208 ± 0.025
1.622TyrVal: 1.622 ± 0.033
0.331TyrTrp: 0.331 ± 0.012
0.482TyrTyr: 0.482 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5193 proteins (1739715 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski