Amino acid dipepetide frequency for Maribacter sp. RZ26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.456AlaAla: 4.456 ± 0.076	0.567AlaCys: 0.567 ± 0.02	3.522AlaAsp: 3.522 ± 0.05	4.177AlaGlu: 4.177 ± 0.059	3.282AlaPhe: 3.282 ± 0.053	4.413AlaGly: 4.413 ± 0.078	1.157AlaHis: 1.157 ± 0.029	5.3AlaIle: 5.3 ± 0.063	4.556AlaLys: 4.556 ± 0.076	6.226AlaLeu: 6.226 ± 0.079	1.601AlaMet: 1.601 ± 0.037	3.529AlaAsn: 3.529 ± 0.06	2.128AlaPro: 2.128 ± 0.048	2.424AlaGln: 2.424 ± 0.042	2.061AlaArg: 2.061 ± 0.038	4.281AlaSer: 4.281 ± 0.055	3.775AlaThr: 3.775 ± 0.069	4.115AlaVal: 4.115 ± 0.069	0.659AlaTrp: 0.659 ± 0.021	2.478AlaTyr: 2.478 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.487CysAla: 0.487 ± 0.019	0.095CysCys: 0.095 ± 0.008	0.44CysAsp: 0.44 ± 0.019	0.484CysGlu: 0.484 ± 0.033	0.426CysPhe: 0.426 ± 0.019	0.593CysGly: 0.593 ± 0.022	0.204CysHis: 0.204 ± 0.013	0.55CysIle: 0.55 ± 0.022	0.449CysLys: 0.449 ± 0.017	0.608CysLeu: 0.608 ± 0.019	0.148CysMet: 0.148 ± 0.01	0.398CysAsn: 0.398 ± 0.02	0.33CysPro: 0.33 ± 0.018	0.222CysGln: 0.222 ± 0.013	0.202CysArg: 0.202 ± 0.012	0.599CysSer: 0.599 ± 0.025	0.502CysThr: 0.502 ± 0.031	0.417CysVal: 0.417 ± 0.017	0.065CysTrp: 0.065 ± 0.007	0.298CysTyr: 0.298 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.969AspAla: 3.969 ± 0.069	0.436AspCys: 0.436 ± 0.019	3.224AspAsp: 3.224 ± 0.062	3.882AspGlu: 3.882 ± 0.055	3.793AspPhe: 3.793 ± 0.052	4.708AspGly: 4.708 ± 0.099	0.869AspHis: 0.869 ± 0.027	4.485AspIle: 4.485 ± 0.06	3.816AspLys: 3.816 ± 0.058	5.326AspLeu: 5.326 ± 0.068	1.277AspMet: 1.277 ± 0.033	3.044AspAsn: 3.044 ± 0.054	1.849AspPro: 1.849 ± 0.047	1.435AspGln: 1.435 ± 0.039	1.926AspArg: 1.926 ± 0.038	3.72AspSer: 3.72 ± 0.062	3.011AspThr: 3.011 ± 0.047	3.667AspVal: 3.667 ± 0.057	0.869AspTrp: 0.869 ± 0.025	2.746AspTyr: 2.746 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	4.344GluAla: 4.344 ± 0.066	0.355GluCys: 0.355 ± 0.017	3.77GluAsp: 3.77 ± 0.054	5.204GluGlu: 5.204 ± 0.083	2.922GluPhe: 2.922 ± 0.041	4.18GluGly: 4.18 ± 0.054	1.172GluHis: 1.172 ± 0.032	5.466GluIle: 5.466 ± 0.058	5.789GluLys: 5.789 ± 0.074	6.357GluLeu: 6.357 ± 0.075	1.724GluMet: 1.724 ± 0.035	4.682GluAsn: 4.682 ± 0.052	1.793GluPro: 1.793 ± 0.037	2.289GluGln: 2.289 ± 0.043	2.751GluArg: 2.751 ± 0.05	3.583GluSer: 3.583 ± 0.051	3.828GluThr: 3.828 ± 0.059	4.713GluVal: 4.713 ± 0.064	0.765GluTrp: 0.765 ± 0.026	2.418GluTyr: 2.418 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.12PheAla: 3.12 ± 0.05	0.407PheCys: 0.407 ± 0.016	3.381PheAsp: 3.381 ± 0.062	3.694PheGlu: 3.694 ± 0.051	2.775PhePhe: 2.775 ± 0.054	3.778PheGly: 3.778 ± 0.056	0.863PheHis: 0.863 ± 0.027	3.515PheIle: 3.515 ± 0.062	3.493PheLys: 3.493 ± 0.053	4.867PheLeu: 4.867 ± 0.074	1.164PheMet: 1.164 ± 0.028	2.942PheAsn: 2.942 ± 0.06	1.737PhePro: 1.737 ± 0.031	1.604PheGln: 1.604 ± 0.04	1.784PheArg: 1.784 ± 0.036	3.845PheSer: 3.845 ± 0.052	3.057PheThr: 3.057 ± 0.054	3.051PheVal: 3.051 ± 0.046	0.643PheTrp: 0.643 ± 0.023	2.083PheTyr: 2.083 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.409GlyAla: 4.409 ± 0.073	0.724GlyCys: 0.724 ± 0.04	3.832GlyAsp: 3.832 ± 0.063	4.049GlyGlu: 4.049 ± 0.057	3.8GlyPhe: 3.8 ± 0.058	5.011GlyGly: 5.011 ± 0.091	1.242GlyHis: 1.242 ± 0.032	5.68GlyIle: 5.68 ± 0.067	5.172GlyLys: 5.172 ± 0.063	6.238GlyLeu: 6.238 ± 0.072	1.741GlyMet: 1.741 ± 0.038	3.873GlyAsn: 3.873 ± 0.067	1.621GlyPro: 1.621 ± 0.036	1.998GlyGln: 1.998 ± 0.039	2.365GlyArg: 2.365 ± 0.045	4.308GlySer: 4.308 ± 0.072	4.174GlyThr: 4.174 ± 0.093	4.433GlyVal: 4.433 ± 0.052	0.957GlyTrp: 0.957 ± 0.031	2.92GlyTyr: 2.92 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.994HisAla: 0.994 ± 0.026	0.158HisCys: 0.158 ± 0.01	0.878HisAsp: 0.878 ± 0.024	0.997HisGlu: 0.997 ± 0.028	1.111HisPhe: 1.111 ± 0.029	1.173HisGly: 1.173 ± 0.033	0.449HisHis: 0.449 ± 0.019	1.341HisIle: 1.341 ± 0.035	1.133HisLys: 1.133 ± 0.029	1.821HisLeu: 1.821 ± 0.04	0.384HisMet: 0.384 ± 0.015	0.873HisAsn: 0.873 ± 0.027	0.881HisPro: 0.881 ± 0.026	0.683HisGln: 0.683 ± 0.022	0.715HisArg: 0.715 ± 0.023	1.095HisSer: 1.095 ± 0.029	0.998HisThr: 0.998 ± 0.024	0.946HisVal: 0.946 ± 0.026	0.253HisTrp: 0.253 ± 0.013	0.77HisTyr: 0.77 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.432IleAla: 5.432 ± 0.067	0.609IleCys: 0.609 ± 0.023	4.751IleAsp: 4.751 ± 0.064	5.134IleGlu: 5.134 ± 0.058	3.414IlePhe: 3.414 ± 0.059	5.151IleGly: 5.151 ± 0.065	1.295IleHis: 1.295 ± 0.03	4.947IleIle: 4.947 ± 0.062	4.942IleLys: 4.942 ± 0.063	6.8IleLeu: 6.8 ± 0.084	1.375IleMet: 1.375 ± 0.035	3.951IleAsn: 3.951 ± 0.06	3.303IlePro: 3.303 ± 0.044	2.544IleGln: 2.544 ± 0.045	2.774IleArg: 2.774 ± 0.038	5.444IleSer: 5.444 ± 0.067	4.544IleThr: 4.544 ± 0.078	4.572IleVal: 4.572 ± 0.053	0.781IleTrp: 0.781 ± 0.027	2.644IleTyr: 2.644 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.75LysAla: 4.75 ± 0.078	0.3LysCys: 0.3 ± 0.017	4.271LysAsp: 4.271 ± 0.061	5.957LysGlu: 5.957 ± 0.062	2.783LysPhe: 2.783 ± 0.041	4.696LysGly: 4.696 ± 0.064	1.319LysHis: 1.319 ± 0.032	5.373LysIle: 5.373 ± 0.072	6.602LysLys: 6.602 ± 0.096	6.193LysLeu: 6.193 ± 0.076	1.899LysMet: 1.899 ± 0.041	4.633LysAsn: 4.633 ± 0.071	2.418LysPro: 2.418 ± 0.042	2.374LysGln: 2.374 ± 0.041	2.933LysArg: 2.933 ± 0.054	4.598LysSer: 4.598 ± 0.064	4.306LysThr: 4.306 ± 0.054	4.612LysVal: 4.612 ± 0.064	0.903LysTrp: 0.903 ± 0.026	2.779LysTyr: 2.779 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	6.116LeuAla: 6.116 ± 0.075	0.702LeuCys: 0.702 ± 0.022	5.341LeuAsp: 5.341 ± 0.061	6.298LeuGlu: 6.298 ± 0.078	4.944LeuPhe: 4.944 ± 0.072	6.208LeuGly: 6.208 ± 0.066	1.49LeuHis: 1.49 ± 0.035	6.377LeuIle: 6.377 ± 0.085	7.451LeuLys: 7.451 ± 0.086	9.044LeuLeu: 9.044 ± 0.111	2.079LeuMet: 2.079 ± 0.04	5.408LeuAsn: 5.408 ± 0.062	3.652LeuPro: 3.652 ± 0.055	3.121LeuGln: 3.121 ± 0.048	3.325LeuArg: 3.325 ± 0.046	6.612LeuSer: 6.612 ± 0.072	5.003LeuThr: 5.003 ± 0.064	5.745LeuVal: 5.745 ± 0.067	0.954LeuTrp: 0.954 ± 0.031	3.06LeuTyr: 3.06 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.756MetAla: 1.756 ± 0.036	0.134MetCys: 0.134 ± 0.009	1.297MetAsp: 1.297 ± 0.032	1.561MetGlu: 1.561 ± 0.038	0.864MetPhe: 0.864 ± 0.042	1.624MetGly: 1.624 ± 0.039	0.412MetHis: 0.412 ± 0.017	1.38MetIle: 1.38 ± 0.035	2.132MetLys: 2.132 ± 0.038	1.966MetLeu: 1.966 ± 0.04	0.565MetMet: 0.565 ± 0.019	1.379MetAsn: 1.379 ± 0.03	0.918MetPro: 0.918 ± 0.028	0.824MetGln: 0.824 ± 0.023	0.92MetArg: 0.92 ± 0.025	1.362MetSer: 1.362 ± 0.031	1.231MetThr: 1.231 ± 0.027	1.57MetVal: 1.57 ± 0.036	0.186MetTrp: 0.186 ± 0.011	0.709MetTyr: 0.709 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.624AsnAla: 3.624 ± 0.058	0.462AsnCys: 0.462 ± 0.025	3.153AsnAsp: 3.153 ± 0.057	3.547AsnGlu: 3.547 ± 0.052	2.949AsnPhe: 2.949 ± 0.049	4.26AsnGly: 4.26 ± 0.083	1.017AsnHis: 1.017 ± 0.026	4.121AsnIle: 4.121 ± 0.054	3.637AsnLys: 3.637 ± 0.063	5.271AsnLeu: 5.271 ± 0.074	1.268AsnMet: 1.268 ± 0.03	3.137AsnAsn: 3.137 ± 0.061	2.853AsnPro: 2.853 ± 0.045	2.234AsnGln: 2.234 ± 0.051	2.232AsnArg: 2.232 ± 0.043	3.812AsnSer: 3.812 ± 0.064	3.487AsnThr: 3.487 ± 0.051	3.285AsnVal: 3.285 ± 0.063	0.771AsnTrp: 0.771 ± 0.031	2.598AsnTyr: 2.598 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.0ProAla: 2.0 ± 0.041	0.206ProCys: 0.206 ± 0.013	2.337ProAsp: 2.337 ± 0.043	3.174ProGlu: 3.174 ± 0.052	1.96ProPhe: 1.96 ± 0.036	2.11ProGly: 2.11 ± 0.045	0.648ProHis: 0.648 ± 0.023	2.828ProIle: 2.828 ± 0.043	2.614ProLys: 2.614 ± 0.04	3.053ProLeu: 3.053 ± 0.054	0.812ProMet: 0.812 ± 0.024	2.316ProAsn: 2.316 ± 0.048	1.013ProPro: 1.013 ± 0.031	1.098ProGln: 1.098 ± 0.026	1.028ProArg: 1.028 ± 0.027	2.376ProSer: 2.376 ± 0.04	2.082ProThr: 2.082 ± 0.044	2.323ProVal: 2.323 ± 0.044	0.413ProTrp: 0.413 ± 0.019	1.41ProTyr: 1.41 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	1.882GlnAla: 1.882 ± 0.039	0.176GlnCys: 0.176 ± 0.011	1.681GlnAsp: 1.681 ± 0.035	2.486GlnGlu: 2.486 ± 0.039	1.581GlnPhe: 1.581 ± 0.035	1.913GlnGly: 1.913 ± 0.036	0.566GlnHis: 0.566 ± 0.022	2.505GlnIle: 2.505 ± 0.037	2.846GlnLys: 2.846 ± 0.051	3.363GlnLeu: 3.363 ± 0.049	0.856GlnMet: 0.856 ± 0.023	2.121GlnAsn: 2.121 ± 0.039	1.078GlnPro: 1.078 ± 0.027	1.284GlnGln: 1.284 ± 0.032	1.252GlnArg: 1.252 ± 0.037	1.943GlnSer: 1.943 ± 0.039	1.77GlnThr: 1.77 ± 0.038	1.983GlnVal: 1.983 ± 0.042	0.416GlnTrp: 0.416 ± 0.018	1.255GlnTyr: 1.255 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	2.191ArgAla: 2.191 ± 0.039	0.195ArgCys: 0.195 ± 0.012	1.935ArgAsp: 1.935 ± 0.039	2.243ArgGlu: 2.243 ± 0.044	2.017ArgPhe: 2.017 ± 0.04	2.118ArgGly: 2.118 ± 0.045	0.633ArgHis: 0.633 ± 0.021	3.071ArgIle: 3.071 ± 0.049	2.902ArgLys: 2.902 ± 0.05	3.399ArgLeu: 3.399 ± 0.051	0.973ArgMet: 0.973 ± 0.026	2.175ArgAsn: 2.175 ± 0.042	1.231ArgPro: 1.231 ± 0.029	1.161ArgGln: 1.161 ± 0.028	1.463ArgArg: 1.463 ± 0.04	2.05ArgSer: 2.05 ± 0.043	2.037ArgThr: 2.037 ± 0.039	2.156ArgVal: 2.156 ± 0.038	0.453ArgTrp: 0.453 ± 0.015	1.6ArgTyr: 1.6 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.882SerAla: 3.882 ± 0.059	0.693SerCys: 0.693 ± 0.026	3.72SerAsp: 3.72 ± 0.057	4.429SerGlu: 4.429 ± 0.06	3.824SerPhe: 3.824 ± 0.065	4.822SerGly: 4.822 ± 0.071	1.054SerHis: 1.054 ± 0.029	5.2SerIle: 5.2 ± 0.059	4.944SerLys: 4.944 ± 0.068	6.028SerLeu: 6.028 ± 0.069	1.379SerMet: 1.379 ± 0.035	3.714SerAsn: 3.714 ± 0.067	2.275SerPro: 2.275 ± 0.042	2.019SerGln: 2.019 ± 0.034	2.202SerArg: 2.202 ± 0.042	4.517SerSer: 4.517 ± 0.069	3.657SerThr: 3.657 ± 0.054	4.046SerVal: 4.046 ± 0.061	0.792SerTrp: 0.792 ± 0.027	2.718SerTyr: 2.718 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	3.863ThrAla: 3.863 ± 0.085	0.379ThrCys: 0.379 ± 0.024	3.516ThrAsp: 3.516 ± 0.063	3.623ThrGlu: 3.623 ± 0.05	2.997ThrPhe: 2.997 ± 0.048	4.183ThrGly: 4.183 ± 0.082	1.022ThrHis: 1.022 ± 0.025	4.618ThrIle: 4.618 ± 0.065	3.679ThrLys: 3.679 ± 0.062	5.278ThrLeu: 5.278 ± 0.057	1.078ThrMet: 1.078 ± 0.028	3.196ThrAsn: 3.196 ± 0.064	2.535ThrPro: 2.535 ± 0.053	1.723ThrGln: 1.723 ± 0.033	1.74ThrArg: 1.74 ± 0.035	3.935ThrSer: 3.935 ± 0.061	3.448ThrThr: 3.448 ± 0.083	3.775ThrVal: 3.775 ± 0.087	0.671ThrTrp: 0.671 ± 0.026	2.337ThrTyr: 2.337 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.284ValAla: 4.284 ± 0.064	0.506ValCys: 0.506 ± 0.022	3.806ValAsp: 3.806 ± 0.056	3.925ValGlu: 3.925 ± 0.056	3.419ValPhe: 3.419 ± 0.053	4.055ValGly: 4.055 ± 0.065	1.119ValHis: 1.119 ± 0.029	4.479ValIle: 4.479 ± 0.055	4.092ValLys: 4.092 ± 0.052	6.288ValLeu: 6.288 ± 0.066	1.357ValMet: 1.357 ± 0.031	3.355ValAsn: 3.355 ± 0.049	2.389ValPro: 2.389 ± 0.043	1.928ValGln: 1.928 ± 0.033	2.123ValArg: 2.123 ± 0.041	4.564ValSer: 4.564 ± 0.074	3.759ValThr: 3.759 ± 0.098	4.355ValVal: 4.355 ± 0.068	0.667ValTrp: 0.667 ± 0.022	2.219ValTyr: 2.219 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.657TrpAla: 0.657 ± 0.022	0.094TrpCys: 0.094 ± 0.008	0.777TrpAsp: 0.777 ± 0.03	0.789TrpGlu: 0.789 ± 0.024	0.618TrpPhe: 0.618 ± 0.022	0.861TrpGly: 0.861 ± 0.024	0.297TrpHis: 0.297 ± 0.014	0.74TrpIle: 0.74 ± 0.024	0.833TrpLys: 0.833 ± 0.026	1.085TrpLeu: 1.085 ± 0.029	0.358TrpMet: 0.358 ± 0.016	0.818TrpAsn: 0.818 ± 0.038	0.322TrpPro: 0.322 ± 0.017	0.455TrpGln: 0.455 ± 0.019	0.477TrpArg: 0.477 ± 0.018	0.742TrpSer: 0.742 ± 0.026	0.616TrpThr: 0.616 ± 0.019	0.728TrpVal: 0.728 ± 0.025	0.174TrpTrp: 0.174 ± 0.013	0.469TrpTyr: 0.469 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.492TyrAla: 2.492 ± 0.041	0.32TyrCys: 0.32 ± 0.014	2.418TyrAsp: 2.418 ± 0.038	2.45TyrGlu: 2.45 ± 0.039	2.325TyrPhe: 2.325 ± 0.039	2.69TyrGly: 2.69 ± 0.051	0.826TyrHis: 0.826 ± 0.024	2.429TyrIle: 2.429 ± 0.042	2.619TyrLys: 2.619 ± 0.041	3.718TyrLeu: 3.718 ± 0.06	0.75TyrMet: 0.75 ± 0.022	2.172TyrAsn: 2.172 ± 0.043	1.475TyrPro: 1.475 ± 0.029	1.54TyrGln: 1.54 ± 0.035	1.73TyrArg: 1.73 ± 0.038	2.53TyrSer: 2.53 ± 0.043	2.313TyrThr: 2.313 ± 0.053	2.197TyrVal: 2.197 ± 0.036	0.518TyrTrp: 0.518 ± 0.018	1.682TyrTyr: 1.682 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4327 proteins (1542337 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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