Amino acid dipepetide frequency for Rhizobiales bacterium IZ6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.771AlaAla: 16.771 ± 0.196
0.844AlaCys: 0.844 ± 0.032
6.512AlaAsp: 6.512 ± 0.082
7.872AlaGlu: 7.872 ± 0.13
4.561AlaPhe: 4.561 ± 0.072
10.283AlaGly: 10.283 ± 0.118
2.12AlaHis: 2.12 ± 0.05
6.206AlaIle: 6.206 ± 0.096
5.359AlaLys: 5.359 ± 0.105
13.307AlaLeu: 13.307 ± 0.153
3.052AlaMet: 3.052 ± 0.063
2.892AlaAsn: 2.892 ± 0.061
5.923AlaPro: 5.923 ± 0.105
3.734AlaGln: 3.734 ± 0.075
8.272AlaArg: 8.272 ± 0.095
6.44AlaSer: 6.44 ± 0.083
5.313AlaThr: 5.313 ± 0.088
8.838AlaVal: 8.838 ± 0.097
1.356AlaTrp: 1.356 ± 0.041
2.407AlaTyr: 2.407 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.865CysAla: 0.865 ± 0.034
0.092CysCys: 0.092 ± 0.01
0.441CysAsp: 0.441 ± 0.023
0.402CysGlu: 0.402 ± 0.019
0.278CysPhe: 0.278 ± 0.017
0.808CysGly: 0.808 ± 0.035
0.182CysHis: 0.182 ± 0.015
0.337CysIle: 0.337 ± 0.019
0.218CysLys: 0.218 ± 0.016
0.682CysLeu: 0.682 ± 0.028
0.151CysMet: 0.151 ± 0.012
0.162CysAsn: 0.162 ± 0.013
0.344CysPro: 0.344 ± 0.022
0.178CysGln: 0.178 ± 0.014
0.525CysArg: 0.525 ± 0.024
0.406CysSer: 0.406 ± 0.019
0.345CysThr: 0.345 ± 0.02
0.526CysVal: 0.526 ± 0.026
0.113CysTrp: 0.113 ± 0.011
0.163CysTyr: 0.163 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.608AspAla: 6.608 ± 0.086
0.376AspCys: 0.376 ± 0.02
2.992AspAsp: 2.992 ± 0.078
3.826AspGlu: 3.826 ± 0.077
2.294AspPhe: 2.294 ± 0.053
5.07AspGly: 5.07 ± 0.118
1.146AspHis: 1.146 ± 0.039
3.429AspIle: 3.429 ± 0.068
2.21AspLys: 2.21 ± 0.051
6.055AspLeu: 6.055 ± 0.09
1.333AspMet: 1.333 ± 0.039
1.314AspAsn: 1.314 ± 0.041
3.417AspPro: 3.417 ± 0.061
1.562AspGln: 1.562 ± 0.04
3.837AspArg: 3.837 ± 0.071
2.252AspSer: 2.252 ± 0.058
2.608AspThr: 2.608 ± 0.058
4.337AspVal: 4.337 ± 0.069
0.919AspTrp: 0.919 ± 0.033
1.444AspTyr: 1.444 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
7.517GluAla: 7.517 ± 0.116
0.314GluCys: 0.314 ± 0.02
3.215GluAsp: 3.215 ± 0.067
3.761GluGlu: 3.761 ± 0.096
2.061GluPhe: 2.061 ± 0.049
4.498GluGly: 4.498 ± 0.07
1.184GluHis: 1.184 ± 0.043
4.093GluIle: 4.093 ± 0.068
3.138GluLys: 3.138 ± 0.063
5.568GluLeu: 5.568 ± 0.088
1.471GluMet: 1.471 ± 0.046
1.799GluAsn: 1.799 ± 0.04
2.856GluPro: 2.856 ± 0.059
1.96GluGln: 1.96 ± 0.051
4.82GluArg: 4.82 ± 0.093
2.483GluSer: 2.483 ± 0.057
3.65GluThr: 3.65 ± 0.066
4.004GluVal: 4.004 ± 0.084
0.684GluTrp: 0.684 ± 0.027
1.014GluTyr: 1.014 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.919PheAla: 4.919 ± 0.08
0.386PheCys: 0.386 ± 0.022
2.652PheAsp: 2.652 ± 0.059
2.303PheGlu: 2.303 ± 0.052
1.681PhePhe: 1.681 ± 0.047
3.941PheGly: 3.941 ± 0.072
0.713PheHis: 0.713 ± 0.029
1.883PheIle: 1.883 ± 0.048
1.264PheLys: 1.264 ± 0.038
3.642PheLeu: 3.642 ± 0.073
0.798PheMet: 0.798 ± 0.031
1.061PheAsn: 1.061 ± 0.037
1.625PhePro: 1.625 ± 0.046
1.041PheGln: 1.041 ± 0.032
2.335PheArg: 2.335 ± 0.05
2.3PheSer: 2.3 ± 0.058
2.102PheThr: 2.102 ± 0.053
2.997PheVal: 2.997 ± 0.068
0.533PheTrp: 0.533 ± 0.026
0.956PheTyr: 0.956 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
9.395GlyAla: 9.395 ± 0.113
0.713GlyCys: 0.713 ± 0.029
4.543GlyAsp: 4.543 ± 0.089
4.914GlyGlu: 4.914 ± 0.078
3.886GlyPhe: 3.886 ± 0.074
7.567GlyGly: 7.567 ± 0.142
1.872GlyHis: 1.872 ± 0.046
4.693GlyIle: 4.693 ± 0.078
3.83GlyLys: 3.83 ± 0.074
8.589GlyLeu: 8.589 ± 0.115
2.173GlyMet: 2.173 ± 0.051
2.191GlyAsn: 2.191 ± 0.066
3.562GlyPro: 3.562 ± 0.066
2.625GlyGln: 2.625 ± 0.064
5.857GlyArg: 5.857 ± 0.092
4.66GlySer: 4.66 ± 0.083
4.839GlyThr: 4.839 ± 0.086
6.251GlyVal: 6.251 ± 0.083
1.228GlyTrp: 1.228 ± 0.041
2.21GlyTyr: 2.21 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.145HisAla: 2.145 ± 0.057
0.174HisCys: 0.174 ± 0.015
1.115HisAsp: 1.115 ± 0.043
1.054HisGlu: 1.054 ± 0.035
0.85HisPhe: 0.85 ± 0.032
1.754HisGly: 1.754 ± 0.043
0.512HisHis: 0.512 ± 0.03
1.029HisIle: 1.029 ± 0.038
0.656HisLys: 0.656 ± 0.027
1.944HisLeu: 1.944 ± 0.053
0.469HisMet: 0.469 ± 0.023
0.487HisAsn: 0.487 ± 0.023
1.248HisPro: 1.248 ± 0.037
0.509HisGln: 0.509 ± 0.022
1.199HisArg: 1.199 ± 0.037
0.901HisSer: 0.901 ± 0.029
0.852HisThr: 0.852 ± 0.034
1.37HisVal: 1.37 ± 0.044
0.273HisTrp: 0.273 ± 0.018
0.527HisTyr: 0.527 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
7.609IleAla: 7.609 ± 0.101
0.484IleCys: 0.484 ± 0.022
3.562IleAsp: 3.562 ± 0.07
3.809IleGlu: 3.809 ± 0.063
2.075IlePhe: 2.075 ± 0.057
5.267IleGly: 5.267 ± 0.084
0.901IleHis: 0.901 ± 0.033
2.461IleIle: 2.461 ± 0.061
1.853IleLys: 1.853 ± 0.052
4.791IleLeu: 4.791 ± 0.088
0.969IleMet: 0.969 ± 0.032
1.43IleAsn: 1.43 ± 0.038
2.461IlePro: 2.461 ± 0.057
1.256IleGln: 1.256 ± 0.036
3.201IleArg: 3.201 ± 0.061
3.112IleSer: 3.112 ± 0.061
2.834IleThr: 2.834 ± 0.062
4.482IleVal: 4.482 ± 0.08
0.576IleTrp: 0.576 ± 0.023
1.158IleTyr: 1.158 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
5.1LysAla: 5.1 ± 0.093
0.186LysCys: 0.186 ± 0.013
2.374LysAsp: 2.374 ± 0.058
2.102LysGlu: 2.102 ± 0.058
1.195LysPhe: 1.195 ± 0.035
3.255LysGly: 3.255 ± 0.066
0.753LysHis: 0.753 ± 0.028
2.468LysIle: 2.468 ± 0.053
2.464LysLys: 2.464 ± 0.07
4.237LysLeu: 4.237 ± 0.068
0.873LysMet: 0.873 ± 0.034
1.288LysAsn: 1.288 ± 0.038
2.663LysPro: 2.663 ± 0.058
1.152LysGln: 1.152 ± 0.034
2.772LysArg: 2.772 ± 0.064
2.382LysSer: 2.382 ± 0.054
2.698LysThr: 2.698 ± 0.055
2.759LysVal: 2.759 ± 0.057
0.417LysTrp: 0.417 ± 0.022
0.742LysTyr: 0.742 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
13.121LeuAla: 13.121 ± 0.157
0.75LeuCys: 0.75 ± 0.029
6.085LeuAsp: 6.085 ± 0.081
5.244LeuGlu: 5.244 ± 0.081
3.82LeuPhe: 3.82 ± 0.077
8.347LeuGly: 8.347 ± 0.098
1.712LeuHis: 1.712 ± 0.045
5.472LeuIle: 5.472 ± 0.085
4.59LeuLys: 4.59 ± 0.072
9.291LeuLeu: 9.291 ± 0.146
2.181LeuMet: 2.181 ± 0.053
2.667LeuAsn: 2.667 ± 0.061
5.258LeuPro: 5.258 ± 0.093
2.533LeuGln: 2.533 ± 0.057
6.19LeuArg: 6.19 ± 0.092
6.292LeuSer: 6.292 ± 0.091
5.595LeuThr: 5.595 ± 0.085
7.543LeuVal: 7.543 ± 0.1
1.093LeuTrp: 1.093 ± 0.038
2.042LeuTyr: 2.042 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.717MetAla: 2.717 ± 0.047
0.137MetCys: 0.137 ± 0.013
1.127MetAsp: 1.127 ± 0.03
1.055MetGlu: 1.055 ± 0.036
0.729MetPhe: 0.729 ± 0.03
1.668MetGly: 1.668 ± 0.05
0.404MetHis: 0.404 ± 0.022
1.283MetIle: 1.283 ± 0.037
1.087MetLys: 1.087 ± 0.04
2.189MetLeu: 2.189 ± 0.054
0.57MetMet: 0.57 ± 0.026
0.785MetAsn: 0.785 ± 0.027
1.469MetPro: 1.469 ± 0.036
0.753MetGln: 0.753 ± 0.028
1.758MetArg: 1.758 ± 0.042
1.673MetSer: 1.673 ± 0.047
1.668MetThr: 1.668 ± 0.044
1.385MetVal: 1.385 ± 0.042
0.201MetTrp: 0.201 ± 0.013
0.305MetTyr: 0.305 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.202AsnAla: 3.202 ± 0.066
0.234AsnCys: 0.234 ± 0.015
1.387AsnAsp: 1.387 ± 0.046
1.397AsnGlu: 1.397 ± 0.039
1.032AsnPhe: 1.032 ± 0.038
2.297AsnGly: 2.297 ± 0.07
0.479AsnHis: 0.479 ± 0.024
1.567AsnIle: 1.567 ± 0.044
0.859AsnLys: 0.859 ± 0.029
2.696AsnLeu: 2.696 ± 0.053
0.598AsnMet: 0.598 ± 0.025
0.712AsnAsn: 0.712 ± 0.034
1.844AsnPro: 1.844 ± 0.039
0.702AsnGln: 0.702 ± 0.03
1.84AsnArg: 1.84 ± 0.046
1.269AsnSer: 1.269 ± 0.044
1.371AsnThr: 1.371 ± 0.046
2.148AsnVal: 2.148 ± 0.054
0.393AsnTrp: 0.393 ± 0.021
0.706AsnTyr: 0.706 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
6.205ProAla: 6.205 ± 0.11
0.263ProCys: 0.263 ± 0.017
3.747ProAsp: 3.747 ± 0.073
3.843ProGlu: 3.843 ± 0.069
2.06ProPhe: 2.06 ± 0.047
4.691ProGly: 4.691 ± 0.078
0.995ProHis: 0.995 ± 0.04
2.311ProIle: 2.311 ± 0.045
2.386ProLys: 2.386 ± 0.064
4.597ProLeu: 4.597 ± 0.079
1.107ProMet: 1.107 ± 0.034
1.503ProAsn: 1.503 ± 0.044
2.944ProPro: 2.944 ± 0.085
1.694ProGln: 1.694 ± 0.047
2.837ProArg: 2.837 ± 0.061
2.803ProSer: 2.803 ± 0.058
2.176ProThr: 2.176 ± 0.046
4.257ProVal: 4.257 ± 0.072
0.614ProTrp: 0.614 ± 0.028
1.139ProTyr: 1.139 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.501GlnAla: 3.501 ± 0.07
0.174GlnCys: 0.174 ± 0.015
1.468GlnAsp: 1.468 ± 0.042
1.501GlnGlu: 1.501 ± 0.048
1.031GlnPhe: 1.031 ± 0.032
2.164GlnGly: 2.164 ± 0.052
0.518GlnHis: 0.518 ± 0.023
1.907GlnIle: 1.907 ± 0.041
1.312GlnLys: 1.312 ± 0.036
2.664GlnLeu: 2.664 ± 0.061
0.749GlnMet: 0.749 ± 0.03
0.932GlnAsn: 0.932 ± 0.033
1.514GlnPro: 1.514 ± 0.045
1.089GlnGln: 1.089 ± 0.039
2.166GlnArg: 2.166 ± 0.045
1.724GlnSer: 1.724 ± 0.044
1.78GlnThr: 1.78 ± 0.049
2.004GlnVal: 2.004 ± 0.052
0.307GlnTrp: 0.307 ± 0.016
0.548GlnTyr: 0.548 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.653ArgAla: 7.653 ± 0.121
0.386ArgCys: 0.386 ± 0.02
3.744ArgAsp: 3.744 ± 0.065
4.179ArgGlu: 4.179 ± 0.077
2.816ArgPhe: 2.816 ± 0.056
5.006ArgGly: 5.006 ± 0.087
1.455ArgHis: 1.455 ± 0.047
3.742ArgIle: 3.742 ± 0.064
2.561ArgLys: 2.561 ± 0.053
7.202ArgLeu: 7.202 ± 0.104
1.687ArgMet: 1.687 ± 0.042
1.703ArgAsn: 1.703 ± 0.05
3.412ArgPro: 3.412 ± 0.071
2.013ArgGln: 2.013 ± 0.054
5.128ArgArg: 5.128 ± 0.098
3.51ArgSer: 3.51 ± 0.067
3.523ArgThr: 3.523 ± 0.062
4.786ArgVal: 4.786 ± 0.073
0.838ArgTrp: 0.838 ± 0.027
1.564ArgTyr: 1.564 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.471SerAla: 6.471 ± 0.097
0.396SerCys: 0.396 ± 0.022
3.192SerAsp: 3.192 ± 0.057
3.23SerGlu: 3.23 ± 0.069
2.385SerPhe: 2.385 ± 0.055
5.581SerGly: 5.581 ± 0.092
1.015SerHis: 1.015 ± 0.029
2.778SerIle: 2.778 ± 0.055
2.007SerLys: 2.007 ± 0.048
5.416SerLeu: 5.416 ± 0.081
1.226SerMet: 1.226 ± 0.033
1.461SerAsn: 1.461 ± 0.044
2.635SerPro: 2.635 ± 0.053
1.582SerGln: 1.582 ± 0.046
3.39SerArg: 3.39 ± 0.05
3.145SerSer: 3.145 ± 0.068
2.634SerThr: 2.634 ± 0.059
4.14SerVal: 4.14 ± 0.062
0.698SerTrp: 0.698 ± 0.03
1.347SerTyr: 1.347 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.305ThrAla: 6.305 ± 0.09
0.389ThrCys: 0.389 ± 0.021
2.807ThrAsp: 2.807 ± 0.059
2.989ThrGlu: 2.989 ± 0.054
2.087ThrPhe: 2.087 ± 0.051
5.23ThrGly: 5.23 ± 0.087
0.964ThrHis: 0.964 ± 0.033
2.851ThrIle: 2.851 ± 0.061
1.962ThrLys: 1.962 ± 0.042
5.517ThrLeu: 5.517 ± 0.075
1.033ThrMet: 1.033 ± 0.035
1.337ThrAsn: 1.337 ± 0.043
3.195ThrPro: 3.195 ± 0.052
1.456ThrGln: 1.456 ± 0.036
3.154ThrArg: 3.154 ± 0.067
2.989ThrSer: 2.989 ± 0.057
2.656ThrThr: 2.656 ± 0.054
4.268ThrVal: 4.268 ± 0.082
0.608ThrTrp: 0.608 ± 0.028
1.216ThrTyr: 1.216 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
8.346ValAla: 8.346 ± 0.116
0.584ValCys: 0.584 ± 0.027
4.074ValAsp: 4.074 ± 0.072
4.717ValGlu: 4.717 ± 0.085
2.871ValPhe: 2.871 ± 0.052
5.243ValGly: 5.243 ± 0.09
1.416ValHis: 1.416 ± 0.04
4.137ValIle: 4.137 ± 0.081
2.856ValLys: 2.856 ± 0.063
7.851ValLeu: 7.851 ± 0.096
1.778ValMet: 1.778 ± 0.045
1.986ValAsn: 1.986 ± 0.055
4.065ValPro: 4.065 ± 0.065
2.089ValGln: 2.089 ± 0.048
5.026ValArg: 5.026 ± 0.076
4.447ValSer: 4.447 ± 0.07
4.599ValThr: 4.599 ± 0.089
5.712ValVal: 5.712 ± 0.092
0.895ValTrp: 0.895 ± 0.027
1.461ValTyr: 1.461 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.167TrpAla: 1.167 ± 0.034
0.125TrpCys: 0.125 ± 0.01
0.578TrpAsp: 0.578 ± 0.029
0.58TrpGlu: 0.58 ± 0.027
0.508TrpPhe: 0.508 ± 0.023
0.873TrpGly: 0.873 ± 0.034
0.292TrpHis: 0.292 ± 0.018
0.666TrpIle: 0.666 ± 0.031
0.541TrpLys: 0.541 ± 0.026
1.403TrpLeu: 1.403 ± 0.037
0.307TrpMet: 0.307 ± 0.017
0.431TrpAsn: 0.431 ± 0.02
0.661TrpPro: 0.661 ± 0.028
0.479TrpGln: 0.479 ± 0.024
0.979TrpArg: 0.979 ± 0.032
0.735TrpSer: 0.735 ± 0.03
0.76TrpThr: 0.76 ± 0.032
0.733TrpVal: 0.733 ± 0.026
0.205TrpTrp: 0.205 ± 0.016
0.257TrpTyr: 0.257 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.447TyrAla: 2.447 ± 0.054
0.211TyrCys: 0.211 ± 0.014
1.415TyrAsp: 1.415 ± 0.042
1.309TyrGlu: 1.309 ± 0.04
0.912TyrPhe: 0.912 ± 0.032
2.056TyrGly: 2.056 ± 0.047
0.436TyrHis: 0.436 ± 0.022
0.978TyrIle: 0.978 ± 0.031
0.726TyrLys: 0.726 ± 0.032
2.187TyrLeu: 2.187 ± 0.056
0.438TyrMet: 0.438 ± 0.024
0.591TyrAsn: 0.591 ± 0.028
1.076TyrPro: 1.076 ± 0.034
0.644TyrGln: 0.644 ± 0.027
1.623TyrArg: 1.623 ± 0.04
1.208TyrSer: 1.208 ± 0.037
1.01TyrThr: 1.01 ± 0.033
1.611TyrVal: 1.611 ± 0.04
0.328TyrTrp: 0.328 ± 0.022
0.599TyrTyr: 0.599 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3159 proteins (948505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski