Nitrincola lacisaponensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Nitrincola

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A063Y4H8|A0A063Y4H8_9GAMM 50S ribosomal protein L14 OS=Nitrincola lacisaponensis OX=267850 GN=rplN PE=3 SV=1
MM1 pKa = 6.86TTDD4 pKa = 3.6LPVGVFYY11 pKa = 11.14ASTTGNTEE19 pKa = 3.7QIAEE23 pKa = 4.5KK24 pKa = 10.02IVAILGPTAHH34 pKa = 6.68LHH36 pKa = 6.34DD37 pKa = 3.89IARR40 pKa = 11.84EE41 pKa = 4.07GFQAVDD47 pKa = 4.19GYY49 pKa = 10.98RR50 pKa = 11.84YY51 pKa = 10.29LIMGIPTWDD60 pKa = 3.41FGEE63 pKa = 4.34LQEE66 pKa = 6.02DD67 pKa = 4.66WACHH71 pKa = 5.29WDD73 pKa = 3.7VLTEE77 pKa = 4.31LDD79 pKa = 3.85LQGVTVALFGLGDD92 pKa = 3.65QIGYY96 pKa = 9.66GEE98 pKa = 4.34WFQDD102 pKa = 4.51AMGLLHH108 pKa = 7.26DD109 pKa = 4.58QLRR112 pKa = 11.84QSGARR117 pKa = 11.84LVGYY121 pKa = 9.25WPVAGYY127 pKa = 11.1AFDD130 pKa = 3.81ASKK133 pKa = 11.2ALTPDD138 pKa = 2.84QQYY141 pKa = 9.36FVGLALDD148 pKa = 3.99EE149 pKa = 5.43DD150 pKa = 4.72GQSGLTDD157 pKa = 3.1EE158 pKa = 5.79RR159 pKa = 11.84LEE161 pKa = 3.95NWLTDD166 pKa = 3.31VCNAFQDD173 pKa = 3.62AA174 pKa = 4.05

Molecular weight: 19.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A063Y6V1|A0A063Y6V1_9GAMM Xanthine dehydrogenase molybdenum binding subunit OS=Nitrincola lacisaponensis OX=267850 GN=ADINL_0759 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.89SLTVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3092

0

3092

1040077

37

6157

336.4

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.689 ± 0.047

1.03 ± 0.016

5.442 ± 0.041

6.114 ± 0.043

3.774 ± 0.028

7.122 ± 0.05

2.342 ± 0.022

5.607 ± 0.037

3.281 ± 0.035

11.851 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.024

3.147 ± 0.031

4.496 ± 0.03

5.333 ± 0.042

6.149 ± 0.042

6.068 ± 0.035

5.062 ± 0.036

6.885 ± 0.039

1.34 ± 0.018

2.665 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski