Amino acid dipepetide frequency for Streptomyces sp. CB01580

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.061AlaAla: 21.061 ± 0.166
1.096AlaCys: 1.096 ± 0.022
8.504AlaAsp: 8.504 ± 0.066
8.745AlaGlu: 8.745 ± 0.068
3.482AlaPhe: 3.482 ± 0.043
12.88AlaGly: 12.88 ± 0.093
3.007AlaHis: 3.007 ± 0.041
3.325AlaIle: 3.325 ± 0.047
2.694AlaLys: 2.694 ± 0.053
14.225AlaLeu: 14.225 ± 0.13
2.552AlaMet: 2.552 ± 0.038
1.851AlaAsn: 1.851 ± 0.032
7.431AlaPro: 7.431 ± 0.09
3.461AlaGln: 3.461 ± 0.039
10.761AlaArg: 10.761 ± 0.085
5.939AlaSer: 5.939 ± 0.054
7.014AlaThr: 7.014 ± 0.066
12.498AlaVal: 12.498 ± 0.079
1.774AlaTrp: 1.774 ± 0.026
2.728AlaTyr: 2.728 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.147CysAla: 1.147 ± 0.026
0.095CysCys: 0.095 ± 0.007
0.496CysAsp: 0.496 ± 0.016
0.421CysGlu: 0.421 ± 0.015
0.248CysPhe: 0.248 ± 0.012
0.993CysGly: 0.993 ± 0.024
0.195CysHis: 0.195 ± 0.009
0.156CysIle: 0.156 ± 0.008
0.11CysLys: 0.11 ± 0.007
0.733CysLeu: 0.733 ± 0.021
0.135CysMet: 0.135 ± 0.008
0.123CysAsn: 0.123 ± 0.008
0.528CysPro: 0.528 ± 0.017
0.166CysGln: 0.166 ± 0.01
0.675CysArg: 0.675 ± 0.018
0.449CysSer: 0.449 ± 0.016
0.541CysThr: 0.541 ± 0.018
0.705CysVal: 0.705 ± 0.017
0.126CysTrp: 0.126 ± 0.008
0.157CysTyr: 0.157 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.888AspAla: 7.888 ± 0.064
0.438AspCys: 0.438 ± 0.015
3.757AspAsp: 3.757 ± 0.049
4.106AspGlu: 4.106 ± 0.049
1.627AspPhe: 1.627 ± 0.031
6.703AspGly: 6.703 ± 0.06
1.471AspHis: 1.471 ± 0.025
1.93AspIle: 1.93 ± 0.033
1.174AspLys: 1.174 ± 0.029
6.111AspLeu: 6.111 ± 0.063
0.846AspMet: 0.846 ± 0.021
0.923AspAsn: 0.923 ± 0.024
4.57AspPro: 4.57 ± 0.051
1.458AspGln: 1.458 ± 0.026
5.25AspArg: 5.25 ± 0.064
2.505AspSer: 2.505 ± 0.036
3.358AspThr: 3.358 ± 0.048
4.744AspVal: 4.744 ± 0.046
1.013AspTrp: 1.013 ± 0.022
1.058AspTyr: 1.058 ± 0.025
0.0AspXaa: 0.0 ± 0.001
Glu
7.414GluAla: 7.414 ± 0.071
0.398GluCys: 0.398 ± 0.013
2.807GluAsp: 2.807 ± 0.037
3.555GluGlu: 3.555 ± 0.049
1.471GluPhe: 1.471 ± 0.026
4.338GluGly: 4.338 ± 0.042
1.557GluHis: 1.557 ± 0.03
2.289GluIle: 2.289 ± 0.035
1.471GluLys: 1.471 ± 0.031
6.834GluLeu: 6.834 ± 0.063
0.886GluMet: 0.886 ± 0.02
1.088GluAsn: 1.088 ± 0.025
3.424GluPro: 3.424 ± 0.051
2.157GluGln: 2.157 ± 0.033
5.719GluArg: 5.719 ± 0.063
2.607GluSer: 2.607 ± 0.038
2.911GluThr: 2.911 ± 0.036
4.351GluVal: 4.351 ± 0.048
0.769GluTrp: 0.769 ± 0.017
1.098GluTyr: 1.098 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.613PheAla: 3.613 ± 0.049
0.278PheCys: 0.278 ± 0.012
2.028PheAsp: 2.028 ± 0.034
1.468PheGlu: 1.468 ± 0.032
0.847PhePhe: 0.847 ± 0.021
3.034PheGly: 3.034 ± 0.038
0.621PheHis: 0.621 ± 0.019
0.758PheIle: 0.758 ± 0.018
0.49PheLys: 0.49 ± 0.015
2.563PheLeu: 2.563 ± 0.043
0.388PheMet: 0.388 ± 0.015
0.554PheAsn: 0.554 ± 0.018
1.404PhePro: 1.404 ± 0.023
0.634PheGln: 0.634 ± 0.019
1.911PheArg: 1.911 ± 0.03
1.451PheSer: 1.451 ± 0.028
1.948PheThr: 1.948 ± 0.028
2.205PheVal: 2.205 ± 0.034
0.42PheTrp: 0.42 ± 0.016
0.569PheTyr: 0.569 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.425GlyAla: 11.425 ± 0.098
0.861GlyCys: 0.861 ± 0.019
5.256GlyAsp: 5.256 ± 0.052
5.122GlyGlu: 5.122 ± 0.057
2.869GlyPhe: 2.869 ± 0.04
9.009GlyGly: 9.009 ± 0.097
2.443GlyHis: 2.443 ± 0.039
3.579GlyIle: 3.579 ± 0.042
2.396GlyLys: 2.396 ± 0.048
9.416GlyLeu: 9.416 ± 0.072
1.988GlyMet: 1.988 ± 0.034
1.818GlyAsn: 1.818 ± 0.037
5.607GlyPro: 5.607 ± 0.068
2.507GlyGln: 2.507 ± 0.041
8.247GlyArg: 8.247 ± 0.071
5.513GlySer: 5.513 ± 0.058
6.548GlyThr: 6.548 ± 0.066
7.445GlyVal: 7.445 ± 0.066
1.646GlyTrp: 1.646 ± 0.031
2.204GlyTyr: 2.204 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.752HisAla: 2.752 ± 0.039
0.231HisCys: 0.231 ± 0.01
1.397HisAsp: 1.397 ± 0.028
1.262HisGlu: 1.262 ± 0.023
0.668HisPhe: 0.668 ± 0.017
2.542HisGly: 2.542 ± 0.042
0.718HisHis: 0.718 ± 0.02
0.704HisIle: 0.704 ± 0.019
0.328HisLys: 0.328 ± 0.012
2.476HisLeu: 2.476 ± 0.037
0.347HisMet: 0.347 ± 0.012
0.35HisAsn: 0.35 ± 0.015
1.843HisPro: 1.843 ± 0.029
0.638HisGln: 0.638 ± 0.018
2.21HisArg: 2.21 ± 0.036
1.005HisSer: 1.005 ± 0.023
1.334HisThr: 1.334 ± 0.026
1.732HisVal: 1.732 ± 0.027
0.402HisTrp: 0.402 ± 0.013
0.477HisTyr: 0.477 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
4.733IleAla: 4.733 ± 0.049
0.327IleCys: 0.327 ± 0.012
2.281IleAsp: 2.281 ± 0.033
2.07IleGlu: 2.07 ± 0.035
0.689IlePhe: 0.689 ± 0.022
3.718IleGly: 3.718 ± 0.055
0.663IleHis: 0.663 ± 0.017
0.83IleIle: 0.83 ± 0.023
0.724IleLys: 0.724 ± 0.022
2.282IleLeu: 2.282 ± 0.032
0.433IleMet: 0.433 ± 0.016
0.686IleAsn: 0.686 ± 0.017
1.733IlePro: 1.733 ± 0.03
0.684IleGln: 0.684 ± 0.019
2.332IleArg: 2.332 ± 0.038
1.661IleSer: 1.661 ± 0.028
2.208IleThr: 2.208 ± 0.035
2.752IleVal: 2.752 ± 0.037
0.389IleTrp: 0.389 ± 0.014
0.491IleTyr: 0.491 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.763LysAla: 2.763 ± 0.051
0.117LysCys: 0.117 ± 0.008
1.354LysAsp: 1.354 ± 0.032
1.195LysGlu: 1.195 ± 0.027
0.473LysPhe: 0.473 ± 0.015
1.839LysGly: 1.839 ± 0.036
0.441LysHis: 0.441 ± 0.015
0.826LysIle: 0.826 ± 0.024
0.885LysLys: 0.885 ± 0.033
1.948LysLeu: 1.948 ± 0.036
0.387LysMet: 0.387 ± 0.014
0.597LysAsn: 0.597 ± 0.02
1.32LysPro: 1.32 ± 0.03
0.663LysGln: 0.663 ± 0.021
1.436LysArg: 1.436 ± 0.026
1.154LysSer: 1.154 ± 0.029
1.203LysThr: 1.203 ± 0.031
1.837LysVal: 1.837 ± 0.033
0.241LysTrp: 0.241 ± 0.011
0.454LysTyr: 0.454 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.67LeuAla: 14.67 ± 0.125
0.847LeuCys: 0.847 ± 0.02
6.913LeuAsp: 6.913 ± 0.067
4.574LeuGlu: 4.574 ± 0.057
2.566LeuPhe: 2.566 ± 0.038
9.223LeuGly: 9.223 ± 0.076
2.301LeuHis: 2.301 ± 0.037
3.294LeuIle: 3.294 ± 0.048
1.974LeuLys: 1.974 ± 0.035
11.121LeuLeu: 11.121 ± 0.104
1.631LeuMet: 1.631 ± 0.033
1.668LeuAsn: 1.668 ± 0.028
6.495LeuPro: 6.495 ± 0.06
2.033LeuGln: 2.033 ± 0.034
8.795LeuArg: 8.795 ± 0.081
5.214LeuSer: 5.214 ± 0.054
6.591LeuThr: 6.591 ± 0.068
8.846LeuVal: 8.846 ± 0.079
1.241LeuTrp: 1.241 ± 0.029
1.829LeuTyr: 1.829 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.265MetAla: 2.265 ± 0.033
0.14MetCys: 0.14 ± 0.008
0.907MetAsp: 0.907 ± 0.019
0.785MetGlu: 0.785 ± 0.02
0.449MetPhe: 0.449 ± 0.015
1.313MetGly: 1.313 ± 0.027
0.355MetHis: 0.355 ± 0.014
0.62MetIle: 0.62 ± 0.017
0.421MetLys: 0.421 ± 0.015
1.721MetLeu: 1.721 ± 0.031
0.313MetMet: 0.313 ± 0.013
0.454MetAsn: 0.454 ± 0.014
1.106MetPro: 1.106 ± 0.027
0.453MetGln: 0.453 ± 0.014
1.5MetArg: 1.5 ± 0.029
1.34MetSer: 1.34 ± 0.024
1.557MetThr: 1.557 ± 0.028
1.316MetVal: 1.316 ± 0.027
0.206MetTrp: 0.206 ± 0.009
0.338MetTyr: 0.338 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.224AsnAla: 2.224 ± 0.037
0.175AsnCys: 0.175 ± 0.01
0.977AsnAsp: 0.977 ± 0.022
0.836AsnGlu: 0.836 ± 0.021
0.463AsnPhe: 0.463 ± 0.017
1.919AsnGly: 1.919 ± 0.037
0.415AsnHis: 0.415 ± 0.014
0.612AsnIle: 0.612 ± 0.018
0.421AsnLys: 0.421 ± 0.018
1.604AsnLeu: 1.604 ± 0.026
0.312AsnMet: 0.312 ± 0.013
0.423AsnAsn: 0.423 ± 0.016
1.315AsnPro: 1.315 ± 0.025
0.467AsnGln: 0.467 ± 0.015
1.325AsnArg: 1.325 ± 0.027
0.868AsnSer: 0.868 ± 0.021
1.109AsnThr: 1.109 ± 0.024
1.365AsnVal: 1.365 ± 0.026
0.293AsnTrp: 0.293 ± 0.013
0.399AsnTyr: 0.399 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
8.657ProAla: 8.657 ± 0.09
0.374ProCys: 0.374 ± 0.014
4.638ProAsp: 4.638 ± 0.055
4.296ProGlu: 4.296 ± 0.05
1.601ProPhe: 1.601 ± 0.025
7.012ProGly: 7.012 ± 0.075
1.42ProHis: 1.42 ± 0.025
1.243ProIle: 1.243 ± 0.026
1.169ProLys: 1.169 ± 0.028
5.387ProLeu: 5.387 ± 0.053
1.036ProMet: 1.036 ± 0.024
0.886ProAsn: 0.886 ± 0.023
3.493ProPro: 3.493 ± 0.07
1.71ProGln: 1.71 ± 0.046
4.087ProArg: 4.087 ± 0.048
3.287ProSer: 3.287 ± 0.039
3.169ProThr: 3.169 ± 0.046
5.817ProVal: 5.817 ± 0.057
0.872ProTrp: 0.872 ± 0.021
1.46ProTyr: 1.46 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.404GlnAla: 3.404 ± 0.047
0.182GlnCys: 0.182 ± 0.009
1.37GlnAsp: 1.37 ± 0.028
1.393GlnGlu: 1.393 ± 0.026
0.668GlnPhe: 0.668 ± 0.018
2.171GlnGly: 2.171 ± 0.038
0.636GlnHis: 0.636 ± 0.019
1.069GlnIle: 1.069 ± 0.027
0.6GlnLys: 0.6 ± 0.02
2.851GlnLeu: 2.851 ± 0.04
0.468GlnMet: 0.468 ± 0.015
0.494GlnAsn: 0.494 ± 0.016
1.579GlnPro: 1.579 ± 0.038
1.2GlnGln: 1.2 ± 0.039
2.274GlnArg: 2.274 ± 0.032
1.131GlnSer: 1.131 ± 0.029
1.233GlnThr: 1.233 ± 0.024
2.19GlnVal: 2.19 ± 0.035
0.432GlnTrp: 0.432 ± 0.014
0.579GlnTyr: 0.579 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
10.352ArgAla: 10.352 ± 0.086
0.644ArgCys: 0.644 ± 0.018
4.462ArgAsp: 4.462 ± 0.049
4.8ArgGlu: 4.8 ± 0.047
2.378ArgPhe: 2.378 ± 0.034
6.05ArgGly: 6.05 ± 0.057
2.167ArgHis: 2.167 ± 0.033
3.508ArgIle: 3.508 ± 0.042
1.649ArgLys: 1.649 ± 0.031
8.914ArgLeu: 8.914 ± 0.091
1.8ArgMet: 1.8 ± 0.029
1.444ArgAsn: 1.444 ± 0.03
5.253ArgPro: 5.253 ± 0.07
2.182ArgGln: 2.182 ± 0.035
7.963ArgArg: 7.963 ± 0.091
4.318ArgSer: 4.318 ± 0.046
5.888ArgThr: 5.888 ± 0.059
5.95ArgVal: 5.95 ± 0.055
1.346ArgTrp: 1.346 ± 0.027
1.773ArgTyr: 1.773 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.668SerAla: 6.668 ± 0.063
0.431SerCys: 0.431 ± 0.017
2.751SerAsp: 2.751 ± 0.035
2.373SerGlu: 2.373 ± 0.038
1.564SerPhe: 1.564 ± 0.031
6.14SerGly: 6.14 ± 0.067
0.992SerHis: 0.992 ± 0.022
1.456SerIle: 1.456 ± 0.028
1.043SerLys: 1.043 ± 0.027
4.754SerLeu: 4.754 ± 0.051
1.098SerMet: 1.098 ± 0.027
0.832SerAsn: 0.832 ± 0.023
3.168SerPro: 3.168 ± 0.039
1.158SerGln: 1.158 ± 0.029
3.783SerArg: 3.783 ± 0.047
2.771SerSer: 2.771 ± 0.047
3.061SerThr: 3.061 ± 0.044
4.403SerVal: 4.403 ± 0.042
0.882SerTrp: 0.882 ± 0.018
1.172SerTyr: 1.172 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.933ThrAla: 8.933 ± 0.078
0.442ThrCys: 0.442 ± 0.016
3.689ThrAsp: 3.689 ± 0.044
3.344ThrGlu: 3.344 ± 0.037
1.585ThrPhe: 1.585 ± 0.03
7.148ThrGly: 7.148 ± 0.07
1.266ThrHis: 1.266 ± 0.024
1.728ThrIle: 1.728 ± 0.028
1.132ThrLys: 1.132 ± 0.029
5.487ThrLeu: 5.487 ± 0.056
0.926ThrMet: 0.926 ± 0.023
0.984ThrAsn: 0.984 ± 0.025
4.044ThrPro: 4.044 ± 0.047
1.243ThrGln: 1.243 ± 0.025
3.951ThrArg: 3.951 ± 0.043
3.166ThrSer: 3.166 ± 0.042
3.961ThrThr: 3.961 ± 0.056
6.136ThrVal: 6.136 ± 0.066
0.866ThrTrp: 0.866 ± 0.024
1.328ThrTyr: 1.328 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.668ValAla: 10.668 ± 0.086
0.795ValCys: 0.795 ± 0.017
5.106ValAsp: 5.106 ± 0.057
4.795ValGlu: 4.795 ± 0.052
2.412ValPhe: 2.412 ± 0.035
6.598ValGly: 6.598 ± 0.065
2.009ValHis: 2.009 ± 0.036
2.734ValIle: 2.734 ± 0.036
1.709ValLys: 1.709 ± 0.031
9.669ValLeu: 9.669 ± 0.072
1.442ValMet: 1.442 ± 0.027
1.566ValAsn: 1.566 ± 0.029
5.506ValPro: 5.506 ± 0.056
2.001ValGln: 2.001 ± 0.032
7.453ValArg: 7.453 ± 0.066
4.295ValSer: 4.295 ± 0.052
5.533ValThr: 5.533 ± 0.056
8.252ValVal: 8.252 ± 0.075
1.074ValTrp: 1.074 ± 0.022
1.543ValTyr: 1.543 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.629TrpAla: 1.629 ± 0.03
0.15TrpCys: 0.15 ± 0.008
0.802TrpAsp: 0.802 ± 0.018
0.718TrpGlu: 0.718 ± 0.021
0.485TrpPhe: 0.485 ± 0.017
1.018TrpGly: 1.018 ± 0.021
0.364TrpHis: 0.364 ± 0.014
0.556TrpIle: 0.556 ± 0.018
0.364TrpLys: 0.364 ± 0.014
1.706TrpLeu: 1.706 ± 0.032
0.294TrpMet: 0.294 ± 0.011
0.388TrpAsn: 0.388 ± 0.015
0.762TrpPro: 0.762 ± 0.016
0.564TrpGln: 0.564 ± 0.017
1.307TrpArg: 1.307 ± 0.027
0.896TrpSer: 0.896 ± 0.023
1.013TrpThr: 1.013 ± 0.022
0.963TrpVal: 0.963 ± 0.021
0.317TrpTrp: 0.317 ± 0.014
0.349TrpTyr: 0.349 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.759TyrAla: 2.759 ± 0.041
0.177TyrCys: 0.177 ± 0.01
1.435TyrAsp: 1.435 ± 0.029
1.285TyrGlu: 1.285 ± 0.026
0.639TyrPhe: 0.639 ± 0.02
2.251TyrGly: 2.251 ± 0.035
0.376TyrHis: 0.376 ± 0.013
0.469TyrIle: 0.469 ± 0.016
0.38TyrLys: 0.38 ± 0.013
2.025TyrLeu: 2.025 ± 0.03
0.278TyrMet: 0.278 ± 0.012
0.393TyrAsn: 0.393 ± 0.016
1.035TyrPro: 1.035 ± 0.021
0.543TyrGln: 0.543 ± 0.016
1.863TyrArg: 1.863 ± 0.03
0.93TyrSer: 0.93 ± 0.024
1.179TyrThr: 1.179 ± 0.026
1.65TyrVal: 1.65 ± 0.026
0.337TyrTrp: 0.337 ± 0.012
0.423TyrTyr: 0.423 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6431 proteins (2125756 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski