Dichomitus squalens (strain LYAD-421) (Western red white-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Dichomitus; Dichomitus squalens

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7SMD7|R7SMD7_DICSQ Uncharacterized protein OS=Dichomitus squalens (strain LYAD-421) OX=732165 GN=DICSQDRAFT_174327 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.18IFAFAAAFALGDD14 pKa = 3.8VLSAVADD21 pKa = 3.83SVDD24 pKa = 3.64PNVPVHH30 pKa = 6.52IFDD33 pKa = 5.07DD34 pKa = 4.39FADD37 pKa = 4.21GLVVGAGYY45 pKa = 10.92APAPGSSLVMTYY57 pKa = 10.57PEE59 pKa = 5.12DD60 pKa = 3.89GFSTFVLTAGTDD72 pKa = 3.57EE73 pKa = 4.55IGQIQLFGSLPRR85 pKa = 11.84DD86 pKa = 3.35APLCMTATNATGTASSHH103 pKa = 5.76PVTLEE108 pKa = 3.59EE109 pKa = 4.98CDD111 pKa = 3.85EE112 pKa = 4.71DD113 pKa = 6.52DD114 pKa = 5.44EE115 pKa = 4.5LQHH118 pKa = 6.2WFVGSASNGTLIRR131 pKa = 11.84PFDD134 pKa = 3.82NVIDD138 pKa = 4.17CLAIPTTSPAVGDD151 pKa = 3.79QVGLQPEE158 pKa = 4.6PCQDD162 pKa = 3.4QLIPRR167 pKa = 11.84QLWHH171 pKa = 7.37AIAAA175 pKa = 4.17

Molecular weight: 18.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7T0T1|R7T0T1_DICSQ SET domain-containing protein OS=Dichomitus squalens (strain LYAD-421) OX=732165 GN=DICSQDRAFT_180325 PE=4 SV=1
MM1 pKa = 7.11ATATVRR7 pKa = 11.84TKK9 pKa = 10.07PAKK12 pKa = 6.38TTRR15 pKa = 11.84AQQPKK20 pKa = 8.51TATFANGNANSNTTLTLHH38 pKa = 6.6LNVNAGAGSRR48 pKa = 11.84AISLGNGNNAAGGRR62 pKa = 11.84RR63 pKa = 11.84TGSSAAGRR71 pKa = 11.84QVV73 pKa = 2.55

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7183

0

7183

2842401

49

3738

395.7

43.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.306 ± 0.03

1.228 ± 0.012

5.645 ± 0.02

5.755 ± 0.026

3.715 ± 0.016

6.676 ± 0.024

2.607 ± 0.014

4.55 ± 0.021

4.032 ± 0.023

9.267 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.01

3.088 ± 0.017

6.714 ± 0.039

3.618 ± 0.02

6.523 ± 0.029

8.298 ± 0.038

6.046 ± 0.019

6.692 ± 0.023

1.547 ± 0.012

2.724 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski