Culex quinquefasciatus (Southern house mosquito) (Culex pungens)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0X7D9|B0X7D9_CULQU Tryptophanyl-tRNA synthetase OS=Culex quinquefasciatus OX=7176 GN=6048677 PE=3 SV=1
MM1 pKa = 7.35SLCKK5 pKa = 10.52AFGKK9 pKa = 10.49DD10 pKa = 3.01KK11 pKa = 11.29VIFATAEE18 pKa = 3.82DD19 pKa = 4.29HH20 pKa = 6.25ATPSTVQLPEE30 pKa = 4.58SEE32 pKa = 4.34PQPGLILDD40 pKa = 4.29NGDD43 pKa = 3.8INWNCPCLGGMATGPCGVEE62 pKa = 3.76FRR64 pKa = 11.84EE65 pKa = 4.39AFSCFHH71 pKa = 6.44YY72 pKa = 10.96SEE74 pKa = 4.93AAPKK78 pKa = 10.83GSDD81 pKa = 3.62CYY83 pKa = 11.37DD84 pKa = 3.28AFKK87 pKa = 10.77TMQDD91 pKa = 3.08CMGNYY96 pKa = 8.81PGVYY100 pKa = 9.77AKK102 pKa = 10.45QNQRR106 pKa = 11.84DD107 pKa = 3.79GDD109 pKa = 4.32DD110 pKa = 4.3DD111 pKa = 6.35DD112 pKa = 6.23EE113 pKa = 7.95DD114 pKa = 5.91DD115 pKa = 5.44GLDD118 pKa = 4.29LGAAMAGAEE127 pKa = 4.18QEE129 pKa = 4.76SPDD132 pKa = 3.96KK133 pKa = 11.44VSVDD137 pKa = 3.7SVDD140 pKa = 3.33ATDD143 pKa = 4.4AGSSAAVVQKK153 pKa = 10.9SVV155 pKa = 2.73

Molecular weight: 16.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0WKZ1|B0WKZ1_CULQU DUF4774 domain-containing protein OS=Culex quinquefasciatus OX=7176 GN=6039874 PE=4 SV=1
MM1 pKa = 7.37TMAKK5 pKa = 10.2LATLLCWLRR14 pKa = 11.84SMNLPPNRR22 pKa = 11.84NRR24 pKa = 11.84PASPSQNQRR33 pKa = 11.84VNPSRR38 pKa = 11.84LVNRR42 pKa = 11.84NPLVNRR48 pKa = 11.84SLLVNRR54 pKa = 11.84NPLASLHH61 pKa = 6.08LQVNPNQWANRR72 pKa = 11.84SQQVNLNQRR81 pKa = 11.84ASQNQQVNLSPRR93 pKa = 11.84VNQKK97 pKa = 10.77LLVNPNQLASLNPTVNPSRR116 pKa = 11.84PVNLNRR122 pKa = 11.84RR123 pKa = 11.84VNSSQQVNLRR133 pKa = 11.84LPLSS137 pKa = 3.73

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19116

88

19204

8459113

29

9108

440.5

49.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.989 ± 0.019

2.037 ± 0.018

5.345 ± 0.013

6.48 ± 0.023

3.948 ± 0.016

6.155 ± 0.021

2.525 ± 0.011

5.059 ± 0.014

5.865 ± 0.023

8.973 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.009

4.604 ± 0.012

5.324 ± 0.024

4.544 ± 0.022

5.685 ± 0.015

7.924 ± 0.025

5.722 ± 0.015

6.471 ± 0.016

1.059 ± 0.007

2.998 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski