Pseudogymnoascus sp. VKM F-3557

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093XV64|A0A093XV64_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-3557 OX=1437433 GN=V490_03263 PE=3 SV=1
MM1 pKa = 7.25VFVKK5 pKa = 10.47PIFALSLVAGLIAPALSAAVRR26 pKa = 11.84INALGDD32 pKa = 4.11SITGSPGCWRR42 pKa = 11.84ALLYY46 pKa = 10.58QKK48 pKa = 10.63LVEE51 pKa = 4.74ANITDD56 pKa = 3.34IDD58 pKa = 4.23FVGTLPGQGCGIEE71 pKa = 3.98YY72 pKa = 10.6DD73 pKa = 4.23GEE75 pKa = 4.34NDD77 pKa = 3.27GHH79 pKa = 7.39GGFLATGIVADD90 pKa = 3.97NQLPGWLAVSQPDD103 pKa = 3.76VVMMQLATNDD113 pKa = 3.04VWSNIATATILDD125 pKa = 4.08AFSTLVDD132 pKa = 3.18QMRR135 pKa = 11.84DD136 pKa = 3.31SKK138 pKa = 10.74STMHH142 pKa = 6.54IVVAQITPMNPTDD155 pKa = 3.49GCATCEE161 pKa = 3.88AGIIALNDD169 pKa = 5.16AIPAWAEE176 pKa = 3.96EE177 pKa = 4.27KK178 pKa = 9.85STTEE182 pKa = 3.79SPITVVDD189 pKa = 4.99CYY191 pKa = 10.57TGYY194 pKa = 8.59DD195 pKa = 3.85TATDD199 pKa = 3.96TYY201 pKa = 11.53DD202 pKa = 3.5GVHH205 pKa = 6.75PNDD208 pKa = 3.7SGNAKK213 pKa = 9.66LAAAWFEE220 pKa = 4.13PLSAAIAAASSS231 pKa = 3.29

Molecular weight: 24.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093XCJ2|A0A093XCJ2_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-3557 OX=1437433 GN=V490_06485 PE=4 SV=1
MM1 pKa = 7.13GHH3 pKa = 6.44SNQGPAGDD11 pKa = 4.45GGSGGAICGGVGRR24 pKa = 11.84LAMQGHH30 pKa = 7.92LLPVDD35 pKa = 4.51SIGGHH40 pKa = 6.26HH41 pKa = 7.29PPPGPYY47 pKa = 9.97FGTDD51 pKa = 3.16PQIFPRR57 pKa = 11.84TAVEE61 pKa = 4.19DD62 pKa = 3.99ADD64 pKa = 3.55AMRR67 pKa = 11.84YY68 pKa = 9.83RR69 pKa = 11.84MLALQAPAVPKK80 pKa = 10.84APAARR85 pKa = 11.84NAPAAQWQNAMPAPLQTSGQSWQTAPAQSMQGHH118 pKa = 5.51MHH120 pKa = 6.69NYY122 pKa = 9.96DD123 pKa = 3.56HH124 pKa = 7.29TSPTPPPKK132 pKa = 9.66RR133 pKa = 11.84SRR135 pKa = 11.84PSRR138 pKa = 11.84PRR140 pKa = 11.84QQPRR144 pKa = 11.84QQAHH148 pKa = 4.81QRR150 pKa = 11.84AATHH154 pKa = 5.64PQIHH158 pKa = 5.57TTIQHH163 pKa = 6.14APVLQAPAQQPGLQASAQQVGPHH186 pKa = 5.82FVQLSTCNCLRR197 pKa = 11.84PISQLSLATPTSFDD211 pKa = 3.19SDD213 pKa = 3.68FFRR216 pKa = 11.84LSACACNILQLSVTFCLRR234 pKa = 11.84LLTMSRR240 pKa = 11.84SFSRR244 pKa = 11.84LPAASLHH251 pKa = 5.81LQTSRR256 pKa = 11.84VQPVLITFILQKK268 pKa = 10.4KK269 pKa = 8.48IKK271 pKa = 9.76NN272 pKa = 3.62

Molecular weight: 29.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9480

0

9480

4561027

8

5968

481.1

53.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.88 ± 0.027

1.209 ± 0.011

5.674 ± 0.023

6.308 ± 0.027

3.649 ± 0.014

7.17 ± 0.023

2.233 ± 0.01

5.112 ± 0.017

5.095 ± 0.022

8.74 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.009

3.78 ± 0.014

5.856 ± 0.024

3.865 ± 0.022

5.769 ± 0.023

8.112 ± 0.027

6.05 ± 0.019

6.138 ± 0.019

1.415 ± 0.009

2.788 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski