Amino acid dipepetide frequency for Pseudobutyrivibrio ruminis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.818AlaAla: 6.818 ± 0.125	1.061AlaCys: 1.061 ± 0.039	4.962AlaAsp: 4.962 ± 0.084	5.039AlaGlu: 5.039 ± 0.095	3.093AlaPhe: 3.093 ± 0.07	5.757AlaGly: 5.757 ± 0.107	1.106AlaHis: 1.106 ± 0.037	5.974AlaIle: 5.974 ± 0.089	5.308AlaLys: 5.308 ± 0.1	6.613AlaLeu: 6.613 ± 0.098	2.478AlaMet: 2.478 ± 0.059	3.335AlaAsn: 3.335 ± 0.072	1.976AlaPro: 1.976 ± 0.055	2.105AlaGln: 2.105 ± 0.059	2.585AlaArg: 2.585 ± 0.059	4.633AlaSer: 4.633 ± 0.131	4.202AlaThr: 4.202 ± 0.084	5.549AlaVal: 5.549 ± 0.082	0.543AlaTrp: 0.543 ± 0.028	2.892AlaTyr: 2.892 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	0.927CysAla: 0.927 ± 0.033	0.211CysCys: 0.211 ± 0.016	0.921CysAsp: 0.921 ± 0.032	0.834CysGlu: 0.834 ± 0.038	0.577CysPhe: 0.577 ± 0.03	1.363CysGly: 1.363 ± 0.047	0.284CysHis: 0.284 ± 0.02	1.104CysIle: 1.104 ± 0.042	0.866CysLys: 0.866 ± 0.03	1.116CysLeu: 1.116 ± 0.039	0.391CysMet: 0.391 ± 0.024	0.627CysAsn: 0.627 ± 0.025	0.57CysPro: 0.57 ± 0.029	0.429CysGln: 0.429 ± 0.022	0.47CysArg: 0.47 ± 0.024	0.802CysSer: 0.802 ± 0.033	0.726CysThr: 0.726 ± 0.034	0.994CysVal: 0.994 ± 0.035	0.099CysTrp: 0.099 ± 0.011	0.582CysTyr: 0.582 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.745AspAla: 4.745 ± 0.078	0.82AspCys: 0.82 ± 0.027	4.185AspAsp: 4.185 ± 0.084	5.668AspGlu: 5.668 ± 0.083	3.088AspPhe: 3.088 ± 0.063	4.812AspGly: 4.812 ± 0.095	0.758AspHis: 0.758 ± 0.03	5.178AspIle: 5.178 ± 0.086	4.198AspLys: 4.198 ± 0.073	4.886AspLeu: 4.886 ± 0.07	2.044AspMet: 2.044 ± 0.052	3.01AspAsn: 3.01 ± 0.067	1.668AspPro: 1.668 ± 0.05	1.202AspGln: 1.202 ± 0.037	2.159AspArg: 2.159 ± 0.053	3.822AspSer: 3.822 ± 0.072	3.341AspThr: 3.341 ± 0.064	4.703AspVal: 4.703 ± 0.089	0.567AspTrp: 0.567 ± 0.03	3.415AspTyr: 3.415 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	5.851GluAla: 5.851 ± 0.102	0.835GluCys: 0.835 ± 0.031	4.814GluAsp: 4.814 ± 0.077	6.37GluGlu: 6.37 ± 0.128	2.772GluPhe: 2.772 ± 0.062	4.224GluGly: 4.224 ± 0.079	1.322GluHis: 1.322 ± 0.039	5.73GluIle: 5.73 ± 0.09	5.593GluLys: 5.593 ± 0.085	6.623GluLeu: 6.623 ± 0.098	2.315GluMet: 2.315 ± 0.05	4.079GluAsn: 4.079 ± 0.067	1.847GluPro: 1.847 ± 0.063	2.389GluGln: 2.389 ± 0.049	2.667GluArg: 2.667 ± 0.065	3.868GluSer: 3.868 ± 0.109	3.583GluThr: 3.583 ± 0.065	4.69GluVal: 4.69 ± 0.091	0.525GluTrp: 0.525 ± 0.028	3.337GluTyr: 3.337 ± 0.074	0.0GluXaa: 0.0 ± 0.0
Phe	3.097PheAla: 3.097 ± 0.062	0.652PheCys: 0.652 ± 0.03	3.002PheAsp: 3.002 ± 0.056	2.886PheGlu: 2.886 ± 0.059	2.043PhePhe: 2.043 ± 0.063	3.09PheGly: 3.09 ± 0.078	0.633PheHis: 0.633 ± 0.028	3.47PheIle: 3.47 ± 0.075	2.489PheLys: 2.489 ± 0.052	3.362PheLeu: 3.362 ± 0.073	1.222PheMet: 1.222 ± 0.04	2.132PheAsn: 2.132 ± 0.051	1.232PhePro: 1.232 ± 0.041	0.994PheGln: 0.994 ± 0.039	1.347PheArg: 1.347 ± 0.034	2.798PheSer: 2.798 ± 0.062	2.585PheThr: 2.585 ± 0.05	3.25PheVal: 3.25 ± 0.072	0.405PheTrp: 0.405 ± 0.023	1.803PheTyr: 1.803 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	5.019GlyAla: 5.019 ± 0.088	1.106GlyCys: 1.106 ± 0.042	3.973GlyAsp: 3.973 ± 0.073	4.385GlyGlu: 4.385 ± 0.076	3.31GlyPhe: 3.31 ± 0.071	4.581GlyGly: 4.581 ± 0.085	1.252GlyHis: 1.252 ± 0.041	5.917GlyIle: 5.917 ± 0.091	5.026GlyLys: 5.026 ± 0.083	5.682GlyLeu: 5.682 ± 0.091	2.257GlyMet: 2.257 ± 0.051	3.12GlyAsn: 3.12 ± 0.065	1.39GlyPro: 1.39 ± 0.045	2.143GlyGln: 2.143 ± 0.06	2.54GlyArg: 2.54 ± 0.057	3.849GlySer: 3.849 ± 0.074	4.163GlyThr: 4.163 ± 0.082	5.125GlyVal: 5.125 ± 0.089	0.656GlyTrp: 0.656 ± 0.033	3.284GlyTyr: 3.284 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	0.958HisAla: 0.958 ± 0.038	0.266HisCys: 0.266 ± 0.018	0.963HisAsp: 0.963 ± 0.039	1.101HisGlu: 1.101 ± 0.042	0.829HisPhe: 0.829 ± 0.032	1.176HisGly: 1.176 ± 0.037	0.353HisHis: 0.353 ± 0.028	1.315HisIle: 1.315 ± 0.043	0.934HisLys: 0.934 ± 0.035	1.325HisLeu: 1.325 ± 0.043	0.484HisMet: 0.484 ± 0.026	0.765HisAsn: 0.765 ± 0.029	0.75HisPro: 0.75 ± 0.035	0.44HisGln: 0.44 ± 0.023	0.646HisArg: 0.646 ± 0.027	0.938HisSer: 0.938 ± 0.036	0.854HisThr: 0.854 ± 0.035	1.144HisVal: 1.144 ± 0.032	0.129HisTrp: 0.129 ± 0.013	0.748HisTyr: 0.748 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.281IleAla: 6.281 ± 0.094	1.317IleCys: 1.317 ± 0.047	5.126IleAsp: 5.126 ± 0.084	5.376IleGlu: 5.376 ± 0.084	3.14IlePhe: 3.14 ± 0.074	5.284IleGly: 5.284 ± 0.08	1.238IleHis: 1.238 ± 0.047	6.466IleIle: 6.466 ± 0.103	5.109IleLys: 5.109 ± 0.079	6.535IleLeu: 6.535 ± 0.091	2.181IleMet: 2.181 ± 0.059	4.057IleAsn: 4.057 ± 0.069	2.858IlePro: 2.858 ± 0.059	2.085IleGln: 2.085 ± 0.046	2.761IleArg: 2.761 ± 0.047	5.63IleSer: 5.63 ± 0.092	4.711IleThr: 4.711 ± 0.089	5.606IleVal: 5.606 ± 0.085	0.554IleTrp: 0.554 ± 0.029	3.098IleTyr: 3.098 ± 0.069	0.0IleXaa: 0.0 ± 0.0
Lys	5.282LysAla: 5.282 ± 0.098	0.789LysCys: 0.789 ± 0.034	4.295LysAsp: 4.295 ± 0.067	6.059LysGlu: 6.059 ± 0.103	2.14LysPhe: 2.14 ± 0.049	3.932LysGly: 3.932 ± 0.074	1.049LysHis: 1.049 ± 0.033	4.789LysIle: 4.789 ± 0.079	5.635LysLys: 5.635 ± 0.1	5.579LysLeu: 5.579 ± 0.083	2.029LysMet: 2.029 ± 0.051	3.804LysAsn: 3.804 ± 0.073	1.932LysPro: 1.932 ± 0.054	2.09LysGln: 2.09 ± 0.056	2.611LysArg: 2.611 ± 0.055	3.68LysSer: 3.68 ± 0.07	3.588LysThr: 3.588 ± 0.079	4.296LysVal: 4.296 ± 0.081	0.549LysTrp: 0.549 ± 0.025	3.298LysTyr: 3.298 ± 0.072	0.0LysXaa: 0.0 ± 0.0
Leu	6.636LeuAla: 6.636 ± 0.108	1.268LeuCys: 1.268 ± 0.04	5.41LeuAsp: 5.41 ± 0.088	5.673LeuGlu: 5.673 ± 0.086	3.506LeuPhe: 3.506 ± 0.073	5.845LeuGly: 5.845 ± 0.093	1.378LeuHis: 1.378 ± 0.044	6.293LeuIle: 6.293 ± 0.09	5.711LeuLys: 5.711 ± 0.08	7.491LeuLeu: 7.491 ± 0.113	2.488LeuMet: 2.488 ± 0.06	4.17LeuAsn: 4.17 ± 0.064	2.862LeuPro: 2.862 ± 0.065	2.409LeuGln: 2.409 ± 0.056	3.021LeuArg: 3.021 ± 0.059	5.981LeuSer: 5.981 ± 0.097	4.716LeuThr: 4.716 ± 0.082	5.659LeuVal: 5.659 ± 0.089	0.663LeuTrp: 0.663 ± 0.033	3.178LeuTyr: 3.178 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.539MetAla: 2.539 ± 0.058	0.354MetCys: 0.354 ± 0.022	2.037MetAsp: 2.037 ± 0.049	2.134MetGlu: 2.134 ± 0.047	1.085MetPhe: 1.085 ± 0.041	2.03MetGly: 2.03 ± 0.052	0.464MetHis: 0.464 ± 0.021	2.281MetIle: 2.281 ± 0.06	2.239MetLys: 2.239 ± 0.048	2.633MetLeu: 2.633 ± 0.065	0.922MetMet: 0.922 ± 0.036	1.6MetAsn: 1.6 ± 0.05	1.066MetPro: 1.066 ± 0.037	0.842MetGln: 0.842 ± 0.03	1.073MetArg: 1.073 ± 0.037	1.901MetSer: 1.901 ± 0.048	1.733MetThr: 1.733 ± 0.048	2.041MetVal: 2.041 ± 0.047	0.214MetTrp: 0.214 ± 0.016	1.051MetTyr: 1.051 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.401AsnAla: 3.401 ± 0.069	0.645AsnCys: 0.645 ± 0.029	2.794AsnAsp: 2.794 ± 0.064	3.385AsnGlu: 3.385 ± 0.066	1.751AsnPhe: 1.751 ± 0.045	3.71AsnGly: 3.71 ± 0.073	0.914AsnHis: 0.914 ± 0.033	4.034AsnIle: 4.034 ± 0.072	3.215AsnLys: 3.215 ± 0.062	3.995AsnLeu: 3.995 ± 0.07	1.519AsnMet: 1.519 ± 0.038	2.514AsnAsn: 2.514 ± 0.063	2.079AsnPro: 2.079 ± 0.052	1.609AsnGln: 1.609 ± 0.045	1.892AsnArg: 1.892 ± 0.052	2.979AsnSer: 2.979 ± 0.06	2.742AsnThr: 2.742 ± 0.058	3.409AsnVal: 3.409 ± 0.059	0.495AsnTrp: 0.495 ± 0.023	2.213AsnTyr: 2.213 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	2.204ProAla: 2.204 ± 0.055	0.347ProCys: 0.347 ± 0.023	2.085ProAsp: 2.085 ± 0.049	2.918ProGlu: 2.918 ± 0.069	1.423ProPhe: 1.423 ± 0.045	1.974ProGly: 1.974 ± 0.053	0.498ProHis: 0.498 ± 0.022	2.37ProIle: 2.37 ± 0.049	1.802ProLys: 1.802 ± 0.052	2.423ProLeu: 2.423 ± 0.053	0.835ProMet: 0.835 ± 0.032	1.433ProAsn: 1.433 ± 0.044	0.538ProPro: 0.538 ± 0.027	0.843ProGln: 0.843 ± 0.04	0.884ProArg: 0.884 ± 0.037	1.738ProSer: 1.738 ± 0.046	1.77ProThr: 1.77 ± 0.049	2.529ProVal: 2.529 ± 0.054	0.302ProTrp: 0.302 ± 0.023	1.374ProTyr: 1.374 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.191GlnAla: 2.191 ± 0.058	0.326GlnCys: 0.326 ± 0.021	1.532GlnAsp: 1.532 ± 0.045	2.087GlnGlu: 2.087 ± 0.054	1.163GlnPhe: 1.163 ± 0.035	1.762GlnGly: 1.762 ± 0.047	0.429GlnHis: 0.429 ± 0.023	2.426GlnIle: 2.426 ± 0.053	1.904GlnLys: 1.904 ± 0.048	2.522GlnLeu: 2.522 ± 0.051	1.028GlnMet: 1.028 ± 0.034	1.316GlnAsn: 1.316 ± 0.041	0.833GlnPro: 0.833 ± 0.037	0.953GlnGln: 0.953 ± 0.036	1.062GlnArg: 1.062 ± 0.037	1.521GlnSer: 1.521 ± 0.043	1.462GlnThr: 1.462 ± 0.042	2.049GlnVal: 2.049 ± 0.045	0.291GlnTrp: 0.291 ± 0.019	1.254GlnTyr: 1.254 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.432ArgAla: 2.432 ± 0.055	0.468ArgCys: 0.468 ± 0.026	2.106ArgAsp: 2.106 ± 0.05	2.718ArgGlu: 2.718 ± 0.064	1.639ArgPhe: 1.639 ± 0.05	2.201ArgGly: 2.201 ± 0.051	0.651ArgHis: 0.651 ± 0.029	3.06ArgIle: 3.06 ± 0.064	2.61ArgLys: 2.61 ± 0.063	3.065ArgLeu: 3.065 ± 0.063	1.174ArgMet: 1.174 ± 0.035	1.802ArgAsn: 1.802 ± 0.043	1.114ArgPro: 1.114 ± 0.04	1.164ArgGln: 1.164 ± 0.038	1.655ArgArg: 1.655 ± 0.053	1.639ArgSer: 1.639 ± 0.04	1.795ArgThr: 1.795 ± 0.047	2.471ArgVal: 2.471 ± 0.051	0.302ArgTrp: 0.302 ± 0.021	1.529ArgTyr: 1.529 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.399SerAla: 4.399 ± 0.123	0.805SerCys: 0.805 ± 0.034	3.996SerAsp: 3.996 ± 0.081	4.299SerGlu: 4.299 ± 0.11	2.941SerPhe: 2.941 ± 0.067	4.47SerGly: 4.47 ± 0.091	1.005SerHis: 1.005 ± 0.041	4.759SerIle: 4.759 ± 0.09	4.024SerLys: 4.024 ± 0.072	5.311SerLeu: 5.311 ± 0.092	1.879SerMet: 1.879 ± 0.052	2.902SerAsn: 2.902 ± 0.063	1.58SerPro: 1.58 ± 0.045	1.75SerGln: 1.75 ± 0.052	2.183SerArg: 2.183 ± 0.05	3.944SerSer: 3.944 ± 0.086	3.532SerThr: 3.532 ± 0.134	4.293SerVal: 4.293 ± 0.083	0.537SerTrp: 0.537 ± 0.029	2.653SerTyr: 2.653 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	4.173ThrAla: 4.173 ± 0.079	0.681ThrCys: 0.681 ± 0.03	3.551ThrAsp: 3.551 ± 0.065	3.773ThrGlu: 3.773 ± 0.075	2.32ThrPhe: 2.32 ± 0.053	4.352ThrGly: 4.352 ± 0.083	0.868ThrHis: 0.868 ± 0.034	4.814ThrIle: 4.814 ± 0.089	3.37ThrLys: 3.37 ± 0.073	4.621ThrLeu: 4.621 ± 0.073	1.517ThrMet: 1.517 ± 0.04	2.476ThrAsn: 2.476 ± 0.056	2.033ThrPro: 2.033 ± 0.053	1.384ThrGln: 1.384 ± 0.037	1.744ThrArg: 1.744 ± 0.051	3.558ThrSer: 3.558 ± 0.124	3.345ThrThr: 3.345 ± 0.078	4.41ThrVal: 4.41 ± 0.092	0.447ThrTrp: 0.447 ± 0.025	2.42ThrTyr: 2.42 ± 0.066	0.0ThrXaa: 0.0 ± 0.0
Val	5.734ValAla: 5.734 ± 0.09	1.165ValCys: 1.165 ± 0.041	4.854ValAsp: 4.854 ± 0.08	5.063ValGlu: 5.063 ± 0.085	3.194ValPhe: 3.194 ± 0.067	4.668ValGly: 4.668 ± 0.077	0.996ValHis: 0.996 ± 0.038	5.573ValIle: 5.573 ± 0.082	4.303ValLys: 4.303 ± 0.081	6.144ValLeu: 6.144 ± 0.105	1.985ValMet: 1.985 ± 0.044	3.396ValAsn: 3.396 ± 0.063	2.303ValPro: 2.303 ± 0.061	1.702ValGln: 1.702 ± 0.044	2.36ValArg: 2.36 ± 0.054	4.78ValSer: 4.78 ± 0.085	4.076ValThr: 4.076 ± 0.084	5.667ValVal: 5.667 ± 0.106	0.517ValTrp: 0.517 ± 0.025	2.867ValTyr: 2.867 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.539TrpAla: 0.539 ± 0.025	0.132TrpCys: 0.132 ± 0.012	0.532TrpAsp: 0.532 ± 0.025	0.514TrpGlu: 0.514 ± 0.025	0.41TrpPhe: 0.41 ± 0.024	0.605TrpGly: 0.605 ± 0.031	0.153TrpHis: 0.153 ± 0.015	0.652TrpIle: 0.652 ± 0.03	0.499TrpLys: 0.499 ± 0.021	0.759TrpLeu: 0.759 ± 0.028	0.28TrpMet: 0.28 ± 0.017	0.507TrpAsn: 0.507 ± 0.03	0.241TrpPro: 0.241 ± 0.018	0.284TrpGln: 0.284 ± 0.018	0.253TrpArg: 0.253 ± 0.018	0.435TrpSer: 0.435 ± 0.023	0.431TrpThr: 0.431 ± 0.026	0.529TrpVal: 0.529 ± 0.025	0.103TrpTrp: 0.103 ± 0.013	0.393TrpTyr: 0.393 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.805TyrAla: 2.805 ± 0.059	0.644TyrCys: 0.644 ± 0.029	3.13TyrAsp: 3.13 ± 0.071	3.154TyrGlu: 3.154 ± 0.07	2.069TyrPhe: 2.069 ± 0.058	2.94TyrGly: 2.94 ± 0.066	0.767TyrHis: 0.767 ± 0.029	3.209TyrIle: 3.209 ± 0.06	2.587TyrLys: 2.587 ± 0.066	3.671TyrLeu: 3.671 ± 0.07	1.208TyrMet: 1.208 ± 0.041	2.25TyrAsn: 2.25 ± 0.053	1.42TyrPro: 1.42 ± 0.04	1.294TyrGln: 1.294 ± 0.04	1.682TyrArg: 1.682 ± 0.047	2.768TyrSer: 2.768 ± 0.064	2.501TyrThr: 2.501 ± 0.07	2.933TyrVal: 2.933 ± 0.065	0.356TyrTrp: 0.356 ± 0.02	2.154TyrTyr: 2.154 ± 0.065	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2521 proteins (841775 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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