Sanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Sanguibacteraceae; Sanguibacter; Sanguibacter keddieii

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1BDW0|D1BDW0_SANKS Assimilatory sulfite reductase (ferredoxin) OS=Sanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74) OX=446469 GN=Sked_32860 PE=4 SV=1
MM1 pKa = 8.26PDD3 pKa = 3.33PNLPAGAAQPPRR15 pKa = 11.84PSARR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84LVRR25 pKa = 11.84AAAAVAGLSLVLAACSSDD43 pKa = 3.15PGTKK47 pKa = 9.86VQTGVDD53 pKa = 3.91GSSPSAGADD62 pKa = 3.41PSDD65 pKa = 4.26GASSAAPSTDD75 pKa = 3.51GIEE78 pKa = 4.64ADD80 pKa = 4.0LLPFYY85 pKa = 9.87EE86 pKa = 4.75QTVEE90 pKa = 3.83WSDD93 pKa = 3.52CGSGFEE99 pKa = 4.47CADD102 pKa = 3.22IEE104 pKa = 5.71VPFDD108 pKa = 3.62WDD110 pKa = 4.03DD111 pKa = 3.7PAADD115 pKa = 4.65TITLGAKK122 pKa = 9.75RR123 pKa = 11.84YY124 pKa = 9.29LSSGEE129 pKa = 3.92RR130 pKa = 11.84KK131 pKa = 8.49GTVLINPGGPGGSGVEE147 pKa = 4.22FVQYY151 pKa = 11.04VPFIFGKK158 pKa = 9.76PLLEE162 pKa = 4.34EE163 pKa = 4.21FDD165 pKa = 3.89VLGFDD170 pKa = 3.66PRR172 pKa = 11.84GVGEE176 pKa = 4.36SSPVACLSDD185 pKa = 3.58AEE187 pKa = 4.63RR188 pKa = 11.84DD189 pKa = 3.57VSDD192 pKa = 3.86AKK194 pKa = 10.48TYY196 pKa = 10.31TPDD199 pKa = 3.65DD200 pKa = 4.06AGLAEE205 pKa = 4.25MQADD209 pKa = 3.63AKK211 pKa = 11.3AFGEE215 pKa = 4.25KK216 pKa = 10.05CLEE219 pKa = 3.96NTGPVLGEE227 pKa = 3.92VDD229 pKa = 3.9TQSSARR235 pKa = 11.84DD236 pKa = 3.38MDD238 pKa = 4.39VIRR241 pKa = 11.84HH242 pKa = 4.62VVGDD246 pKa = 3.65AQLNYY251 pKa = 10.73LGFSYY256 pKa = 8.7GTQLGATYY264 pKa = 10.75AGLYY268 pKa = 8.48PEE270 pKa = 4.36NVGRR274 pKa = 11.84MVLDD278 pKa = 3.67GAIDD282 pKa = 3.79LRR284 pKa = 11.84LTDD287 pKa = 4.88HH288 pKa = 6.83EE289 pKa = 4.81MSLQQAVGFEE299 pKa = 4.09NALTAFVEE307 pKa = 4.49DD308 pKa = 4.42CLAGSGCPLTGTTDD322 pKa = 3.13EE323 pKa = 5.21ALAQVTGLLDD333 pKa = 3.65QILANPMPTGDD344 pKa = 3.75PARR347 pKa = 11.84PLTQTLAFYY356 pKa = 10.78GIAQPLYY363 pKa = 11.06SEE365 pKa = 5.3LLWPQLTAALDD376 pKa = 3.74AAISDD381 pKa = 4.05GDD383 pKa = 4.01GSGLLASADD392 pKa = 3.9SYY394 pKa = 11.85NSRR397 pKa = 11.84NSDD400 pKa = 2.49GTYY403 pKa = 10.78ADD405 pKa = 3.65NQGEE409 pKa = 4.26AFRR412 pKa = 11.84AINCLDD418 pKa = 3.58SRR420 pKa = 11.84SDD422 pKa = 3.54ADD424 pKa = 3.71MATLDD429 pKa = 3.96AEE431 pKa = 4.38ADD433 pKa = 4.28EE434 pKa = 4.99IVAAAPTVGEE444 pKa = 4.04FFTYY448 pKa = 10.5GGLGCADD455 pKa = 3.4WPFPQAEE462 pKa = 4.39KK463 pKa = 10.25TGDD466 pKa = 3.58LAASGAAPILVIGTTNDD483 pKa = 3.39PATPYY488 pKa = 10.5VWAQGLAEE496 pKa = 3.94QLEE499 pKa = 4.66SGVLVTYY506 pKa = 10.27EE507 pKa = 4.5GEE509 pKa = 3.9GHH511 pKa = 5.47TAYY514 pKa = 10.2GQSNSCVLDD523 pKa = 3.51TVDD526 pKa = 5.94AYY528 pKa = 10.16FTDD531 pKa = 3.8GTVPDD536 pKa = 4.43EE537 pKa = 4.61GLVCC541 pKa = 5.22

Molecular weight: 56.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1BH02|D1BH02_SANKS Bifunctional protein PyrR OS=Sanguibacter keddieii (strain ATCC 51767 / DSM 10542 / NCFB 3025 / ST-74) OX=446469 GN=pyrR PE=3 SV=1
MM1 pKa = 7.02SAVRR5 pKa = 11.84RR6 pKa = 11.84LVARR10 pKa = 11.84IDD12 pKa = 3.68LFIVSLLLAVAVASFFPVRR31 pKa = 11.84GQAAEE36 pKa = 4.09GLDD39 pKa = 3.65VAVVIAVGWLFFLYY53 pKa = 10.13GVRR56 pKa = 11.84LPTKK60 pKa = 9.31EE61 pKa = 3.93AVNALRR67 pKa = 11.84DD68 pKa = 3.61WRR70 pKa = 11.84LHH72 pKa = 5.42GSVLAVTFVVFPLLGLAMQLVPSSVVPTDD101 pKa = 3.84LKK103 pKa = 11.18LGLLFLTLLPSTVQSSIAFTSIARR127 pKa = 11.84GNVAGAVVAASFSNLAGVLLTPLLVAGLLGGTVGFSADD165 pKa = 3.71SIGKK169 pKa = 9.61IGLQLLAPFVAGQLLRR185 pKa = 11.84RR186 pKa = 11.84WLAPFVLRR194 pKa = 11.84HH195 pKa = 5.61KK196 pKa = 10.61RR197 pKa = 11.84VTTLTDD203 pKa = 2.96RR204 pKa = 11.84GSVVLVVYY212 pKa = 10.24AAFSHH217 pKa = 6.14GVVDD221 pKa = 5.95GIWTTVDD228 pKa = 2.83VGSIVAVAVLCCLLLAAVLGLTTFAGKK255 pKa = 10.49RR256 pKa = 11.84FGFSRR261 pKa = 11.84ADD263 pKa = 3.46RR264 pKa = 11.84VVLLMCGSKK273 pKa = 10.36KK274 pKa = 10.56SLASGVPMATVLLPAATIGFMVLPLMIFHH303 pKa = 6.86QIQLIVCAVIARR315 pKa = 11.84RR316 pKa = 11.84MGDD319 pKa = 3.13AAEE322 pKa = 4.65PEE324 pKa = 4.32PARR327 pKa = 11.84PP328 pKa = 3.52

Molecular weight: 34.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3710

0

3710

1274206

27

3648

343.5

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.484 ± 0.049

0.516 ± 0.009

6.518 ± 0.037

5.551 ± 0.038

2.613 ± 0.026

9.13 ± 0.042

2.019 ± 0.018

3.148 ± 0.031

1.531 ± 0.026

10.206 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.643 ± 0.016

1.511 ± 0.021

5.734 ± 0.039

2.836 ± 0.021

7.122 ± 0.041

5.979 ± 0.03

6.987 ± 0.054

10.202 ± 0.049

1.448 ± 0.017

1.822 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski