Callipepla squamata (Scaled quail)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A226N589|A0A226N589_CALSU Netrin receptor UNC5 OS=Callipepla squamata OX=9009 GN=ASZ78_010820 PE=3 SV=1
YYY2 pKa = 9.28DDD4 pKa = 3.53YYY6 pKa = 11.66AFCLSTVTNYYY17 pKa = 10.95NVIEEE22 pKa = 4.87NSDDD26 pKa = 3.31DDD28 pKa = 6.01DD29 pKa = 3.63EE30 pKa = 4.89DD31 pKa = 3.5KK32 pKa = 11.53HHH34 pKa = 6.82VEEE37 pKa = 4.84EE38 pKa = 4.18EE39 pKa = 4.42IADDD43 pKa = 4.27ADDD46 pKa = 3.86DDD48 pKa = 3.99SVPEEE53 pKa = 5.27DD54 pKa = 4.9DD55 pKa = 5.72PTDDD59 pKa = 3.44HH60 pKa = 7.03VLPEEE65 pKa = 3.68SEEE68 pKa = 4.46EEE70 pKa = 4.15STNSCWEEE78 pKa = 4.21DD79 pKa = 3.04EE80 pKa = 4.26KKK82 pKa = 9.57EE83 pKa = 3.96KKK85 pKa = 10.17EE86 pKa = 3.68LGPEEE91 pKa = 4.06QSDDD95 pKa = 4.14EE96 pKa = 4.49GCTVKKK102 pKa = 10.56EE103 pKa = 4.27DD104 pKa = 3.82EE105 pKa = 5.04DDD107 pKa = 3.28DDD109 pKa = 5.57EEE111 pKa = 4.27EEE113 pKa = 4.21NHHH116 pKa = 6.57DD117 pKa = 3.89NVEEE121 pKa = 3.95EE122 pKa = 5.53LQQEEE127 pKa = 4.53DD128 pKa = 4.03AVIYYY133 pKa = 10.37EEE135 pKa = 4.87PEEE138 pKa = 3.86EE139 pKa = 4.09DD140 pKa = 3.7RR141 pKa = 11.84QGTPEEE147 pKa = 4.14SGQDDD152 pKa = 3.64EE153 pKa = 4.34GEEE156 pKa = 4.24YY157 pKa = 9.49TSSVHHH163 pKa = 5.69KKK165 pKa = 10.73KK166 pKa = 10.16VLST

Molecular weight: 18.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A226MFK8|A0A226MFK8_CALSU MPN domain-containing protein (Fragment) OS=Callipepla squamata OX=9009 GN=ASZ78_005586 PE=3 SV=1
MM1 pKa = 7.55TFLLFNNLFQKK12 pKa = 10.19SLKK15 pKa = 9.71QRR17 pKa = 11.84LGKK20 pKa = 10.77SNIQARR26 pKa = 11.84LGRR29 pKa = 11.84PAGPLARR36 pKa = 11.84GAMGGRR42 pKa = 11.84GLAMGQRR49 pKa = 11.84GLPRR53 pKa = 11.84GAMRR57 pKa = 11.84GGRR60 pKa = 11.84GARR63 pKa = 11.84ALLRR67 pKa = 11.84GGLPLRR73 pKa = 11.84GQGLLRR79 pKa = 11.84GGRR82 pKa = 11.84GMSPRR87 pKa = 11.84MGLRR91 pKa = 11.84RR92 pKa = 11.84GGIRR96 pKa = 11.84GRR98 pKa = 11.84GGPGRR103 pKa = 11.84GAMGRR108 pKa = 11.84GAMGRR113 pKa = 11.84GGLGGRR119 pKa = 11.84GQWAGTT125 pKa = 3.58

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16973

0

16973

8302635

11

17299

489.2

54.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.02

2.249 ± 0.018

4.991 ± 0.013

7.348 ± 0.028

3.726 ± 0.015

6.199 ± 0.024

2.484 ± 0.008

4.589 ± 0.016

5.992 ± 0.027

9.773 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.009

3.832 ± 0.014

5.576 ± 0.027

4.636 ± 0.019

5.455 ± 0.016

8.281 ± 0.026

5.238 ± 0.017

6.35 ± 0.016

1.166 ± 0.007

2.819 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski