Amino acid dipepetide frequency for Eubacteriaceae bacterium CHKCI004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.004AlaAla: 8.004 ± 0.118	1.104AlaCys: 1.104 ± 0.033	4.628AlaAsp: 4.628 ± 0.063	5.753AlaGlu: 5.753 ± 0.086	3.058AlaPhe: 3.058 ± 0.058	6.49AlaGly: 6.49 ± 0.087	1.081AlaHis: 1.081 ± 0.026	4.706AlaIle: 4.706 ± 0.08	4.427AlaLys: 4.427 ± 0.063	6.8AlaLeu: 6.8 ± 0.097	2.342AlaMet: 2.342 ± 0.048	2.332AlaAsn: 2.332 ± 0.047	2.109AlaPro: 2.109 ± 0.045	2.365AlaGln: 2.365 ± 0.049	3.361AlaArg: 3.361 ± 0.058	3.949AlaSer: 3.949 ± 0.063	2.983AlaThr: 2.983 ± 0.07	6.264AlaVal: 6.264 ± 0.095	0.591AlaTrp: 0.591 ± 0.022	2.574AlaTyr: 2.574 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.016CysAla: 1.016 ± 0.029	0.275CysCys: 0.275 ± 0.017	0.855CysAsp: 0.855 ± 0.028	0.906CysGlu: 0.906 ± 0.031	0.624CysPhe: 0.624 ± 0.022	1.475CysGly: 1.475 ± 0.04	0.36CysHis: 0.36 ± 0.016	1.069CysIle: 1.069 ± 0.03	0.728CysLys: 0.728 ± 0.028	1.259CysLeu: 1.259 ± 0.037	0.441CysMet: 0.441 ± 0.021	0.503CysAsn: 0.503 ± 0.025	0.652CysPro: 0.652 ± 0.026	0.485CysGln: 0.485 ± 0.022	0.87CysArg: 0.87 ± 0.029	0.869CysSer: 0.869 ± 0.026	0.742CysThr: 0.742 ± 0.025	1.026CysVal: 1.026 ± 0.031	0.118CysTrp: 0.118 ± 0.01	0.554CysTyr: 0.554 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.42AspAla: 4.42 ± 0.064	0.888AspCys: 0.888 ± 0.029	3.037AspAsp: 3.037 ± 0.076	4.714AspGlu: 4.714 ± 0.063	2.573AspPhe: 2.573 ± 0.05	4.492AspGly: 4.492 ± 0.081	0.855AspHis: 0.855 ± 0.028	4.538AspIle: 4.538 ± 0.057	3.481AspLys: 3.481 ± 0.064	4.387AspLeu: 4.387 ± 0.066	1.904AspMet: 1.904 ± 0.037	2.314AspAsn: 2.314 ± 0.045	1.64AspPro: 1.64 ± 0.044	1.268AspGln: 1.268 ± 0.033	2.597AspArg: 2.597 ± 0.049	3.344AspSer: 3.344 ± 0.064	3.231AspThr: 3.231 ± 0.063	3.901AspVal: 3.901 ± 0.064	0.588AspTrp: 0.588 ± 0.023	2.879AspTyr: 2.879 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.541GluAla: 5.541 ± 0.083	0.805GluCys: 0.805 ± 0.031	4.608GluAsp: 4.608 ± 0.07	8.334GluGlu: 8.334 ± 0.13	2.388GluPhe: 2.388 ± 0.044	4.76GluGly: 4.76 ± 0.065	1.45GluHis: 1.45 ± 0.036	5.604GluIle: 5.604 ± 0.073	6.959GluLys: 6.959 ± 0.086	6.587GluLeu: 6.587 ± 0.097	2.648GluMet: 2.648 ± 0.053	4.319GluAsn: 4.319 ± 0.075	2.038GluPro: 2.038 ± 0.043	3.26GluGln: 3.26 ± 0.062	3.827GluArg: 3.827 ± 0.076	3.555GluSer: 3.555 ± 0.056	4.082GluThr: 4.082 ± 0.076	4.359GluVal: 4.359 ± 0.068	0.632GluTrp: 0.632 ± 0.026	3.359GluTyr: 3.359 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.91PheAla: 2.91 ± 0.057	0.812PheCys: 0.812 ± 0.024	2.483PheAsp: 2.483 ± 0.046	2.5PheGlu: 2.5 ± 0.05	2.014PhePhe: 2.014 ± 0.055	2.897PheGly: 2.897 ± 0.052	0.882PheHis: 0.882 ± 0.031	2.649PheIle: 2.649 ± 0.058	1.814PheLys: 1.814 ± 0.04	4.101PheLeu: 4.101 ± 0.071	1.13PheMet: 1.13 ± 0.032	1.278PheAsn: 1.278 ± 0.032	1.401PhePro: 1.401 ± 0.03	1.351PheGln: 1.351 ± 0.036	1.991PheArg: 1.991 ± 0.036	2.876PheSer: 2.876 ± 0.052	2.372PheThr: 2.372 ± 0.053	2.684PheVal: 2.684 ± 0.055	0.39PheTrp: 0.39 ± 0.021	1.789PheTyr: 1.789 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.831GlyAla: 4.831 ± 0.086	1.259GlyCys: 1.259 ± 0.035	3.565GlyAsp: 3.565 ± 0.058	5.122GlyGlu: 5.122 ± 0.082	2.83GlyPhe: 2.83 ± 0.048	4.843GlyGly: 4.843 ± 0.087	1.204GlyHis: 1.204 ± 0.039	5.753GlyIle: 5.753 ± 0.094	5.337GlyLys: 5.337 ± 0.068	5.417GlyLeu: 5.417 ± 0.078	2.314GlyMet: 2.314 ± 0.037	3.114GlyAsn: 3.114 ± 0.052	1.232GlyPro: 1.232 ± 0.033	2.435GlyGln: 2.435 ± 0.049	3.565GlyArg: 3.565 ± 0.058	4.258GlySer: 4.258 ± 0.078	4.324GlyThr: 4.324 ± 0.076	4.838GlyVal: 4.838 ± 0.065	0.717GlyTrp: 0.717 ± 0.029	3.277GlyTyr: 3.277 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.062HisAla: 1.062 ± 0.029	0.32HisCys: 0.32 ± 0.019	0.921HisAsp: 0.921 ± 0.038	1.084HisGlu: 1.084 ± 0.033	0.886HisPhe: 0.886 ± 0.026	1.339HisGly: 1.339 ± 0.032	0.452HisHis: 0.452 ± 0.027	1.421HisIle: 1.421 ± 0.036	0.902HisLys: 0.902 ± 0.028	1.613HisLeu: 1.613 ± 0.04	0.587HisMet: 0.587 ± 0.023	0.727HisAsn: 0.727 ± 0.026	0.977HisPro: 0.977 ± 0.033	0.628HisGln: 0.628 ± 0.025	0.917HisArg: 0.917 ± 0.029	1.038HisSer: 1.038 ± 0.03	0.934HisThr: 0.934 ± 0.029	1.071HisVal: 1.071 ± 0.033	0.179HisTrp: 0.179 ± 0.014	0.801HisTyr: 0.801 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.332IleAla: 5.332 ± 0.082	1.267IleCys: 1.267 ± 0.039	3.832IleAsp: 3.832 ± 0.062	4.441IleGlu: 4.441 ± 0.059	2.981IlePhe: 2.981 ± 0.058	4.786IleGly: 4.786 ± 0.08	1.396IleHis: 1.396 ± 0.039	4.911IleIle: 4.911 ± 0.082	3.843IleLys: 3.843 ± 0.062	6.848IleLeu: 6.848 ± 0.103	1.859IleMet: 1.859 ± 0.045	2.7IleAsn: 2.7 ± 0.053	3.055IlePro: 3.055 ± 0.048	2.307IleGln: 2.307 ± 0.043	4.066IleArg: 4.066 ± 0.067	4.839IleSer: 4.839 ± 0.07	3.998IleThr: 3.998 ± 0.067	4.614IleVal: 4.614 ± 0.074	0.602IleTrp: 0.602 ± 0.025	2.896IleTyr: 2.896 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	4.786LysAla: 4.786 ± 0.082	0.728LysCys: 0.728 ± 0.025	3.775LysAsp: 3.775 ± 0.062	6.74LysGlu: 6.74 ± 0.095	1.652LysPhe: 1.652 ± 0.042	3.96LysGly: 3.96 ± 0.062	1.012LysHis: 1.012 ± 0.033	4.808LysIle: 4.808 ± 0.064	6.894LysLys: 6.894 ± 0.108	4.94LysLeu: 4.94 ± 0.075	2.226LysMet: 2.226 ± 0.042	3.647LysAsn: 3.647 ± 0.055	1.857LysPro: 1.857 ± 0.038	2.313LysGln: 2.313 ± 0.052	3.311LysArg: 3.311 ± 0.057	3.182LysSer: 3.182 ± 0.054	3.732LysThr: 3.732 ± 0.059	4.085LysVal: 4.085 ± 0.069	0.589LysTrp: 0.589 ± 0.024	2.766LysTyr: 2.766 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	6.658LeuAla: 6.658 ± 0.079	1.478LeuCys: 1.478 ± 0.039	4.962LeuAsp: 4.962 ± 0.068	6.049LeuGlu: 6.049 ± 0.072	3.907LeuPhe: 3.907 ± 0.067	5.401LeuGly: 5.401 ± 0.089	1.71LeuHis: 1.71 ± 0.046	5.483LeuIle: 5.483 ± 0.086	5.88LeuLys: 5.88 ± 0.082	8.849LeuLeu: 8.849 ± 0.145	2.683LeuMet: 2.683 ± 0.052	3.543LeuAsn: 3.543 ± 0.052	3.683LeuPro: 3.683 ± 0.057	2.975LeuGln: 2.975 ± 0.054	4.352LeuArg: 4.352 ± 0.073	6.581LeuSer: 6.581 ± 0.091	5.067LeuThr: 5.067 ± 0.073	4.945LeuVal: 4.945 ± 0.066	0.741LeuTrp: 0.741 ± 0.024	3.41LeuTyr: 3.41 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.424MetAla: 2.424 ± 0.053	0.33MetCys: 0.33 ± 0.017	1.988MetAsp: 1.988 ± 0.043	3.011MetGlu: 3.011 ± 0.06	1.074MetPhe: 1.074 ± 0.033	2.006MetGly: 2.006 ± 0.047	0.501MetHis: 0.501 ± 0.019	2.087MetIle: 2.087 ± 0.046	2.49MetLys: 2.49 ± 0.043	2.596MetLeu: 2.596 ± 0.047	0.931MetMet: 0.931 ± 0.031	1.523MetAsn: 1.523 ± 0.041	1.098MetPro: 1.098 ± 0.032	1.044MetGln: 1.044 ± 0.028	1.458MetArg: 1.458 ± 0.034	1.694MetSer: 1.694 ± 0.04	1.686MetThr: 1.686 ± 0.04	1.782MetVal: 1.782 ± 0.043	0.204MetTrp: 0.204 ± 0.014	0.929MetTyr: 0.929 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.033AsnAla: 3.033 ± 0.052	0.619AsnCys: 0.619 ± 0.023	2.099AsnAsp: 2.099 ± 0.044	2.669AsnGlu: 2.669 ± 0.049	1.523AsnPhe: 1.523 ± 0.034	3.231AsnGly: 3.231 ± 0.07	0.839AsnHis: 0.839 ± 0.03	3.257AsnIle: 3.257 ± 0.054	2.424AsnLys: 2.424 ± 0.06	3.7AsnLeu: 3.7 ± 0.063	1.274AsnMet: 1.274 ± 0.032	1.909AsnAsn: 1.909 ± 0.059	1.952AsnPro: 1.952 ± 0.047	1.493AsnGln: 1.493 ± 0.039	2.223AsnArg: 2.223 ± 0.047	2.164AsnSer: 2.164 ± 0.054	2.382AsnThr: 2.382 ± 0.055	2.925AsnVal: 2.925 ± 0.063	0.424AsnTrp: 0.424 ± 0.02	1.761AsnTyr: 1.761 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	2.716ProAla: 2.716 ± 0.054	0.436ProCys: 0.436 ± 0.02	2.576ProAsp: 2.576 ± 0.052	3.713ProGlu: 3.713 ± 0.062	1.501ProPhe: 1.501 ± 0.033	2.381ProGly: 2.381 ± 0.051	0.59ProHis: 0.59 ± 0.025	1.786ProIle: 1.786 ± 0.038	1.778ProLys: 1.778 ± 0.043	2.693ProLeu: 2.693 ± 0.051	0.872ProMet: 0.872 ± 0.03	1.056ProAsn: 1.056 ± 0.032	0.83ProPro: 0.83 ± 0.027	1.088ProGln: 1.088 ± 0.03	1.106ProArg: 1.106 ± 0.032	1.849ProSer: 1.849 ± 0.037	1.49ProThr: 1.49 ± 0.04	3.064ProVal: 3.064 ± 0.054	0.306ProTrp: 0.306 ± 0.016	1.501ProTyr: 1.501 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.551GlnAla: 2.551 ± 0.057	0.415GlnCys: 0.415 ± 0.017	1.599GlnAsp: 1.599 ± 0.033	2.911GlnGlu: 2.911 ± 0.053	1.195GlnPhe: 1.195 ± 0.033	1.998GlnGly: 1.998 ± 0.046	0.522GlnHis: 0.522 ± 0.023	2.664GlnIle: 2.664 ± 0.05	2.815GlnLys: 2.815 ± 0.054	2.826GlnLeu: 2.826 ± 0.057	1.223GlnMet: 1.223 ± 0.039	1.678GlnAsn: 1.678 ± 0.043	1.041GlnPro: 1.041 ± 0.028	1.349GlnGln: 1.349 ± 0.043	1.643GlnArg: 1.643 ± 0.042	1.805GlnSer: 1.805 ± 0.043	1.863GlnThr: 1.863 ± 0.041	2.066GlnVal: 2.066 ± 0.046	0.39GlnTrp: 0.39 ± 0.02	1.537GlnTyr: 1.537 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.052ArgAla: 3.052 ± 0.052	0.656ArgCys: 0.656 ± 0.026	2.423ArgAsp: 2.423 ± 0.046	4.482ArgGlu: 4.482 ± 0.086	1.996ArgPhe: 1.996 ± 0.043	2.689ArgGly: 2.689 ± 0.055	0.901ArgHis: 0.901 ± 0.03	3.701ArgIle: 3.701 ± 0.064	4.127ArgLys: 4.127 ± 0.07	4.482ArgLeu: 4.482 ± 0.066	1.783ArgMet: 1.783 ± 0.04	2.274ArgAsn: 2.274 ± 0.044	1.555ArgPro: 1.555 ± 0.044	2.23ArgGln: 2.23 ± 0.047	2.987ArgArg: 2.987 ± 0.063	2.446ArgSer: 2.446 ± 0.053	2.503ArgThr: 2.503 ± 0.055	2.694ArgVal: 2.694 ± 0.055	0.476ArgTrp: 0.476 ± 0.023	2.112ArgTyr: 2.112 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.297SerAla: 4.297 ± 0.06	0.867SerCys: 0.867 ± 0.029	3.389SerAsp: 3.389 ± 0.066	4.167SerGlu: 4.167 ± 0.063	2.751SerPhe: 2.751 ± 0.052	5.317SerGly: 5.317 ± 0.09	1.059SerHis: 1.059 ± 0.034	3.937SerIle: 3.937 ± 0.071	2.858SerLys: 2.858 ± 0.05	5.24SerLeu: 5.24 ± 0.073	1.752SerMet: 1.752 ± 0.043	2.151SerAsn: 2.151 ± 0.057	1.819SerPro: 1.819 ± 0.045	1.922SerGln: 1.922 ± 0.041	3.187SerArg: 3.187 ± 0.057	3.781SerSer: 3.781 ± 0.069	2.741SerThr: 2.741 ± 0.056	4.398SerVal: 4.398 ± 0.065	0.574SerTrp: 0.574 ± 0.022	2.499SerTyr: 2.499 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.669ThrAla: 4.669 ± 0.071	0.657ThrCys: 0.657 ± 0.024	3.346ThrAsp: 3.346 ± 0.058	4.025ThrGlu: 4.025 ± 0.063	2.186ThrPhe: 2.186 ± 0.052	4.681ThrGly: 4.681 ± 0.08	0.847ThrHis: 0.847 ± 0.025	3.805ThrIle: 3.805 ± 0.063	2.895ThrLys: 2.895 ± 0.06	4.854ThrLeu: 4.854 ± 0.074	1.417ThrMet: 1.417 ± 0.035	1.843ThrAsn: 1.843 ± 0.046	2.148ThrPro: 2.148 ± 0.045	1.47ThrGln: 1.47 ± 0.037	2.095ThrArg: 2.095 ± 0.045	2.936ThrSer: 2.936 ± 0.066	2.809ThrThr: 2.809 ± 0.074	4.597ThrVal: 4.597 ± 0.086	0.488ThrTrp: 0.488 ± 0.022	1.964ThrTyr: 1.964 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.335ValAla: 4.335 ± 0.081	1.138ValCys: 1.138 ± 0.032	3.785ValAsp: 3.785 ± 0.065	4.675ValGlu: 4.675 ± 0.06	2.896ValPhe: 2.896 ± 0.051	3.961ValGly: 3.961 ± 0.066	1.118ValHis: 1.118 ± 0.035	4.967ValIle: 4.967 ± 0.068	4.403ValLys: 4.403 ± 0.064	6.452ValLeu: 6.452 ± 0.076	1.99ValMet: 1.99 ± 0.042	2.716ValAsn: 2.716 ± 0.051	2.571ValPro: 2.571 ± 0.048	2.134ValGln: 2.134 ± 0.043	3.302ValArg: 3.302 ± 0.057	4.628ValSer: 4.628 ± 0.065	4.092ValThr: 4.092 ± 0.097	4.458ValVal: 4.458 ± 0.078	0.609ValTrp: 0.609 ± 0.023	2.626ValTyr: 2.626 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.513TrpAla: 0.513 ± 0.02	0.164TrpCys: 0.164 ± 0.013	0.522TrpAsp: 0.522 ± 0.024	0.73TrpGlu: 0.73 ± 0.023	0.381TrpPhe: 0.381 ± 0.018	0.568TrpGly: 0.568 ± 0.022	0.166TrpHis: 0.166 ± 0.011	0.643TrpIle: 0.643 ± 0.026	0.764TrpLys: 0.764 ± 0.029	0.892TrpLeu: 0.892 ± 0.032	0.342TrpMet: 0.342 ± 0.019	0.529TrpAsn: 0.529 ± 0.019	0.185TrpPro: 0.185 ± 0.012	0.388TrpGln: 0.388 ± 0.019	0.409TrpArg: 0.409 ± 0.019	0.484TrpSer: 0.484 ± 0.025	0.423TrpThr: 0.423 ± 0.02	0.488TrpVal: 0.488 ± 0.021	0.1TrpTrp: 0.1 ± 0.01	0.4TrpTyr: 0.4 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.771TyrAla: 2.771 ± 0.046	0.611TyrCys: 0.611 ± 0.023	2.657TyrAsp: 2.657 ± 0.047	3.09TyrGlu: 3.09 ± 0.053	1.909TyrPhe: 1.909 ± 0.042	2.959TyrGly: 2.959 ± 0.05	0.91TyrHis: 0.91 ± 0.025	2.782TyrIle: 2.782 ± 0.048	2.187TyrLys: 2.187 ± 0.041	3.82TyrLeu: 3.82 ± 0.069	1.14TyrMet: 1.14 ± 0.031	1.745TyrAsn: 1.745 ± 0.037	1.487TyrPro: 1.487 ± 0.037	1.586TyrGln: 1.586 ± 0.042	2.302TyrArg: 2.302 ± 0.042	2.432TyrSer: 2.432 ± 0.045	2.277TyrThr: 2.277 ± 0.057	2.598TyrVal: 2.598 ± 0.05	0.372TyrTrp: 0.372 ± 0.018	1.967TyrTyr: 1.967 ± 0.058	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3682 proteins (1165227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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