Streptococcus satellite phage Javan260

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZJI8|A0A4D5ZJI8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan260 OX=2558596 GN=JavanS260_0011 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.68DD12 pKa = 4.04DD13 pKa = 4.01TDD15 pKa = 3.86GANAPYY21 pKa = 10.03FVTFPDD27 pKa = 4.61FEE29 pKa = 5.3HH30 pKa = 7.07SATQGEE36 pKa = 4.54DD37 pKa = 3.02MANAMAMASDD47 pKa = 3.31WLGIHH52 pKa = 6.56LADD55 pKa = 4.36YY56 pKa = 10.61IEE58 pKa = 4.46NGRR61 pKa = 11.84DD62 pKa = 3.13IPTPAPINTLSLADD76 pKa = 3.94NNPFRR81 pKa = 11.84DD82 pKa = 3.99DD83 pKa = 3.55EE84 pKa = 5.07DD85 pKa = 4.4IEE87 pKa = 5.57LIYY90 pKa = 10.9DD91 pKa = 3.72PSKK94 pKa = 11.33SFISMVMVDD103 pKa = 3.25VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.11TLGRR129 pKa = 11.84DD130 pKa = 3.39LGLNFSQTLTDD141 pKa = 5.45AIADD145 pKa = 4.03KK146 pKa = 10.56KK147 pKa = 10.31IHH149 pKa = 5.92AA150 pKa = 5.0

Molecular weight:
16.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZM32|A0A4D5ZM32_9VIRU DNA primase OS=Streptococcus satellite phage Javan260 OX=2558596 GN=JavanS260_0006 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.09IKK4 pKa = 10.66PITKK8 pKa = 9.17KK9 pKa = 10.51NGAIVYY15 pKa = 8.3RR16 pKa = 11.84ANVYY20 pKa = 10.65LGTDD24 pKa = 2.95QVTGKK29 pKa = 9.97KK30 pKa = 10.33AKK32 pKa = 9.51TSVTGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.76EE42 pKa = 3.84VKK44 pKa = 10.02QKK46 pKa = 10.96AKK48 pKa = 10.04HH49 pKa = 5.68AQDD52 pKa = 3.62EE53 pKa = 5.21FISNGYY59 pKa = 7.35TVTKK63 pKa = 9.88VVPIKK68 pKa = 10.67NYY70 pKa = 10.29QEE72 pKa = 4.68LAEE75 pKa = 4.74LWLEE79 pKa = 4.32SYY81 pKa = 10.85QLTVKK86 pKa = 9.85PQTFIATKK94 pKa = 10.24RR95 pKa = 11.84MLYY98 pKa = 10.01NHH100 pKa = 7.89LIPVFGALKK109 pKa = 10.32LNKK112 pKa = 10.01LSVSYY117 pKa = 10.64IQGFINDD124 pKa = 4.13LSAEE128 pKa = 4.15LVHH131 pKa = 6.38YY132 pKa = 10.48SVVHH136 pKa = 5.68SINRR140 pKa = 11.84RR141 pKa = 11.84VLQYY145 pKa = 10.88GVSLQLLPFNPARR158 pKa = 11.84DD159 pKa = 3.54VMLPKK164 pKa = 10.25VPKK167 pKa = 10.2KK168 pKa = 9.64EE169 pKa = 3.73NKK171 pKa = 9.48AIKK174 pKa = 10.16FIAPEE179 pKa = 3.97DD180 pKa = 3.88LKK182 pKa = 11.69ALMAYY187 pKa = 8.24MEE189 pKa = 4.47KK190 pKa = 10.52LANKK194 pKa = 9.07KK195 pKa = 9.8FSYY198 pKa = 10.51FFDD201 pKa = 3.67YY202 pKa = 11.08VLYY205 pKa = 10.68SVLLATGCRR214 pKa = 11.84FGEE217 pKa = 4.58VVALEE222 pKa = 4.12WSDD225 pKa = 3.32IDD227 pKa = 5.09LEE229 pKa = 4.52NGTISITKK237 pKa = 9.93NYY239 pKa = 10.09SRR241 pKa = 11.84LLKK244 pKa = 10.89LIGTPKK250 pKa = 9.91SKK252 pKa = 10.87AGVRR256 pKa = 11.84VISIDD261 pKa = 3.23KK262 pKa = 9.23KK263 pKa = 9.18TINLLRR269 pKa = 11.84LYY271 pKa = 10.58KK272 pKa = 10.31NRR274 pKa = 11.84QRR276 pKa = 11.84QIFIEE281 pKa = 4.08TGARR285 pKa = 11.84VSAVVFATPTKK296 pKa = 9.74EE297 pKa = 4.11YY298 pKa = 10.79QNMATRR304 pKa = 11.84QEE306 pKa = 4.21SLDD309 pKa = 3.62RR310 pKa = 11.84RR311 pKa = 11.84ITEE314 pKa = 3.41IGIPRR319 pKa = 11.84FTFHH323 pKa = 7.74AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.49RR346 pKa = 11.84LGHH349 pKa = 6.19ATLAMTMDD357 pKa = 4.28IYY359 pKa = 11.69SHH361 pKa = 7.7LSMDD365 pKa = 3.69KK366 pKa = 10.11EE367 pKa = 4.32KK368 pKa = 10.95EE369 pKa = 3.93AVSYY373 pKa = 9.5YY374 pKa = 10.62EE375 pKa = 4.12KK376 pKa = 10.7AINSLL381 pKa = 3.57

Molecular weight:
43.52 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3076

37

507

146.5

16.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.722 ± 0.649

0.65 ± 0.202

6.404 ± 0.547

7.38 ± 0.497

3.771 ± 0.289

4.714 ± 0.376

1.658 ± 0.231

7.412 ± 0.373

8.713 ± 0.568

10.468 ± 0.49

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.286

5.592 ± 0.346

3.771 ± 0.475

4.551 ± 0.398

4.876 ± 0.394

5.234 ± 0.372

6.014 ± 0.304

5.169 ± 0.481

0.943 ± 0.15

4.389 ± 0.36

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski