Podoviridae sp. ctpVR23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W650|A0A5Q2W650_9CAUD Uncharacterized protein OS=Podoviridae sp. ctpVR23 OX=2656714 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.32APYY5 pKa = 9.72PNRR8 pKa = 11.84YY9 pKa = 8.24QVEE12 pKa = 4.39QIRR15 pKa = 11.84KK16 pKa = 8.74LAEE19 pKa = 3.68QGLDD23 pKa = 3.04ADD25 pKa = 4.67EE26 pKa = 5.06ISRR29 pKa = 11.84QIQVDD34 pKa = 3.8VEE36 pKa = 4.49GVLVHH41 pKa = 6.83LEE43 pKa = 3.79QLEE46 pKa = 4.47DD47 pKa = 3.76IEE49 pKa = 6.08LSDD52 pKa = 4.16PEE54 pKa = 4.18QGSEE58 pKa = 4.14EE59 pKa = 4.23EE60 pKa = 4.17

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WCC4|A0A5Q2WCC4_9CAUD Uncharacterized protein OS=Podoviridae sp. ctpVR23 OX=2656714 PE=4 SV=1
MM1 pKa = 7.36GSAAGGFRR9 pKa = 11.84RR10 pKa = 11.84SATDD14 pKa = 3.6SIFMGTWSCPVTSTPLRR31 pKa = 11.84GRR33 pKa = 11.84YY34 pKa = 7.45FF35 pKa = 3.39

Molecular weight: 3.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11604

30

1006

252.3

28.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.152 ± 0.503

1.198 ± 0.204

6.36 ± 0.231

6.463 ± 0.266

3.464 ± 0.189

6.782 ± 0.328

1.767 ± 0.203

5.722 ± 0.234

5.653 ± 0.35

7.385 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.102 ± 0.172

4.361 ± 0.153

4.033 ± 0.226

3.878 ± 0.283

5.886 ± 0.274

6.705 ± 0.365

5.989 ± 0.413

7.118 ± 0.283

1.698 ± 0.177

3.283 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski