Clostridiales bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2AYN2|A0A1W2AYN2_9FIRM Diguanylate cyclase (GGDEF) domain-containing protein OS=Clostridiales bacterium OX=1898207 GN=SAMN06297397_1809 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.62KK3 pKa = 8.91MLCFILAAAMMFSITALAEE22 pKa = 4.34DD23 pKa = 4.73SPGGTPPDD31 pKa = 4.2GFSGTPPEE39 pKa = 4.47GTPPDD44 pKa = 3.87GFGGGTPPDD53 pKa = 3.83GTPPDD58 pKa = 3.94GFGGGTPPDD67 pKa = 4.06GAPGGFGGGTPPGGGTSSFEE87 pKa = 4.05YY88 pKa = 10.47AASTEE93 pKa = 4.2VTEE96 pKa = 4.27AAEE99 pKa = 4.36LNSQSFVSEE108 pKa = 4.19TADD111 pKa = 3.79EE112 pKa = 4.4SALLINTADD121 pKa = 3.32TVTITEE127 pKa = 4.03PAITKK132 pKa = 9.46TGDD135 pKa = 2.94SSGGDD140 pKa = 2.97NCNFYY145 pKa = 11.2GLNAALLVMGGSTTTITGGTIEE167 pKa = 4.47TSASGANGVFSYY179 pKa = 10.76GGNGGRR185 pKa = 11.84NGAAGDD191 pKa = 3.74GTTVVISDD199 pKa = 3.59TTIVTDD205 pKa = 3.83GDD207 pKa = 3.93GSGGIMTTGGGITYY221 pKa = 10.38ADD223 pKa = 3.75NLAVTTSGRR232 pKa = 11.84SSAAIRR238 pKa = 11.84TDD240 pKa = 3.07RR241 pKa = 11.84GGGTVVVDD249 pKa = 3.78GGTYY253 pKa = 6.18TTSGLGSPAIYY264 pKa = 8.92STADD268 pKa = 2.89ITVNNASLVSNLSEE282 pKa = 4.28GVCIEE287 pKa = 3.9GMNSITLEE295 pKa = 3.99NSNLTASNTQCNGNATFHH313 pKa = 6.17DD314 pKa = 4.5TIMIYY319 pKa = 10.77QSMSGDD325 pKa = 3.59ADD327 pKa = 3.69SGTSSFTMTGGSLTSLSGHH346 pKa = 5.84MFHH349 pKa = 6.55VTNTHH354 pKa = 6.77AVITLNGVEE363 pKa = 4.96LSNQGSDD370 pKa = 2.98ILLSVCDD377 pKa = 4.98DD378 pKa = 3.55GWHH381 pKa = 6.08GASNVAEE388 pKa = 4.2LVTHH392 pKa = 5.94NQQLEE397 pKa = 4.26GTILAGDD404 pKa = 3.69NSVLKK409 pKa = 9.56LTLSDD414 pKa = 3.64GSAFEE419 pKa = 4.38GTVSGRR425 pKa = 11.84IEE427 pKa = 3.81NAKK430 pKa = 10.52NEE432 pKa = 4.49LISTEE437 pKa = 4.08VGSVSVTLDD446 pKa = 3.33STSTWTLTADD456 pKa = 3.12TWIDD460 pKa = 3.4AFEE463 pKa = 5.05GDD465 pKa = 3.55ASRR468 pKa = 11.84VTGNGYY474 pKa = 7.02TLYY477 pKa = 11.31VNGTALEE484 pKa = 4.97GISS487 pKa = 3.56

Molecular weight: 48.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2BXG4|A0A1W2BXG4_9FIRM Phosphate propanoyltransferase OS=Clostridiales bacterium OX=1898207 GN=SAMN06297397_2445 PE=3 SV=1
MM1 pKa = 7.25FRR3 pKa = 11.84TYY5 pKa = 9.97QPKK8 pKa = 9.54KK9 pKa = 7.56RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.87GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.64EE41 pKa = 3.53LTVV44 pKa = 3.27

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3011

0

3011

1014911

34

4155

337.1

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.436 ± 0.053

1.549 ± 0.019

5.928 ± 0.034

7.477 ± 0.057

3.895 ± 0.03

7.459 ± 0.043

1.749 ± 0.018

6.307 ± 0.045

5.414 ± 0.046

9.327 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.025

3.877 ± 0.034

4.07 ± 0.028

2.977 ± 0.023

5.33 ± 0.041

5.552 ± 0.038

5.825 ± 0.049

6.77 ± 0.033

1.231 ± 0.017

3.808 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski