Amino acid dipepetide frequency for Clostridiales bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.465AlaAla: 9.465 ± 0.136	1.334AlaCys: 1.334 ± 0.037	5.28AlaAsp: 5.28 ± 0.074	7.4AlaGlu: 7.4 ± 0.121	3.561AlaPhe: 3.561 ± 0.067	7.102AlaGly: 7.102 ± 0.092	1.201AlaHis: 1.201 ± 0.041	4.384AlaIle: 4.384 ± 0.08	3.906AlaLys: 3.906 ± 0.067	8.192AlaLeu: 8.192 ± 0.116	2.577AlaMet: 2.577 ± 0.057	2.411AlaAsn: 2.411 ± 0.05	2.855AlaPro: 2.855 ± 0.068	2.363AlaGln: 2.363 ± 0.05	3.908AlaArg: 3.908 ± 0.08	4.153AlaSer: 4.153 ± 0.065	3.322AlaThr: 3.322 ± 0.054	7.241AlaVal: 7.241 ± 0.114	1.063AlaTrp: 1.063 ± 0.036	2.639AlaTyr: 2.639 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	1.32CysAla: 1.32 ± 0.038	0.284CysCys: 0.284 ± 0.016	0.89CysAsp: 0.89 ± 0.031	0.909CysGlu: 0.909 ± 0.032	0.74CysPhe: 0.74 ± 0.028	1.61CysGly: 1.61 ± 0.044	0.294CysHis: 0.294 ± 0.018	0.89CysIle: 0.89 ± 0.032	0.608CysLys: 0.608 ± 0.024	1.442CysLeu: 1.442 ± 0.036	0.437CysMet: 0.437 ± 0.02	0.48CysAsn: 0.48 ± 0.023	0.71CysPro: 0.71 ± 0.03	0.334CysGln: 0.334 ± 0.019	0.951CysArg: 0.951 ± 0.037	0.919CysSer: 0.919 ± 0.034	0.795CysThr: 0.795 ± 0.028	1.078CysVal: 1.078 ± 0.036	0.211CysTrp: 0.211 ± 0.012	0.587CysTyr: 0.587 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.748AspAla: 4.748 ± 0.087	0.826AspCys: 0.826 ± 0.028	3.17AspAsp: 3.17 ± 0.067	4.837AspGlu: 4.837 ± 0.074	2.285AspPhe: 2.285 ± 0.052	5.297AspGly: 5.297 ± 0.108	1.103AspHis: 1.103 ± 0.036	3.697AspIle: 3.697 ± 0.067	2.879AspLys: 2.879 ± 0.061	5.28AspLeu: 5.28 ± 0.074	1.776AspMet: 1.776 ± 0.047	2.141AspAsn: 2.141 ± 0.049	2.922AspPro: 2.922 ± 0.062	1.615AspGln: 1.615 ± 0.039	3.327AspArg: 3.327 ± 0.059	3.045AspSer: 3.045 ± 0.059	3.331AspThr: 3.331 ± 0.054	3.575AspVal: 3.575 ± 0.071	0.823AspTrp: 0.823 ± 0.031	2.599AspTyr: 2.599 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.422GluAla: 6.422 ± 0.096	0.887GluCys: 0.887 ± 0.032	4.44GluAsp: 4.44 ± 0.082	7.086GluGlu: 7.086 ± 0.132	2.034GluPhe: 2.034 ± 0.054	5.014GluGly: 5.014 ± 0.079	1.286GluHis: 1.286 ± 0.037	4.761GluIle: 4.761 ± 0.074	5.783GluLys: 5.783 ± 0.093	6.157GluLeu: 6.157 ± 0.09	2.495GluMet: 2.495 ± 0.056	3.734GluAsn: 3.734 ± 0.065	2.666GluPro: 2.666 ± 0.075	2.787GluGln: 2.787 ± 0.061	3.408GluArg: 3.408 ± 0.065	3.334GluSer: 3.334 ± 0.059	4.723GluThr: 4.723 ± 0.081	3.844GluVal: 3.844 ± 0.067	0.88GluTrp: 0.88 ± 0.034	3.031GluTyr: 3.031 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	3.073PheAla: 3.073 ± 0.069	0.781PheCys: 0.781 ± 0.032	2.49PheAsp: 2.49 ± 0.053	2.312PheGlu: 2.312 ± 0.05	1.819PhePhe: 1.819 ± 0.045	3.074PheGly: 3.074 ± 0.058	0.76PheHis: 0.76 ± 0.027	2.377PheIle: 2.377 ± 0.054	1.206PheLys: 1.206 ± 0.033	3.692PheLeu: 3.692 ± 0.071	1.11PheMet: 1.11 ± 0.033	1.37PheAsn: 1.37 ± 0.042	1.596PhePro: 1.596 ± 0.043	0.99PheGln: 0.99 ± 0.032	2.53PheArg: 2.53 ± 0.051	2.883PheSer: 2.883 ± 0.059	2.486PheThr: 2.486 ± 0.06	2.43PheVal: 2.43 ± 0.052	0.48PheTrp: 0.48 ± 0.024	1.489PheTyr: 1.489 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.484GlyAla: 5.484 ± 0.08	1.307GlyCys: 1.307 ± 0.047	3.896GlyAsp: 3.896 ± 0.073	5.336GlyGlu: 5.336 ± 0.094	3.277GlyPhe: 3.277 ± 0.062	5.173GlyGly: 5.173 ± 0.095	1.345GlyHis: 1.345 ± 0.038	5.541GlyIle: 5.541 ± 0.082	5.057GlyLys: 5.057 ± 0.08	6.387GlyLeu: 6.387 ± 0.09	2.575GlyMet: 2.575 ± 0.054	3.158GlyAsn: 3.158 ± 0.077	1.639GlyPro: 1.639 ± 0.044	2.146GlyGln: 2.146 ± 0.046	3.76GlyArg: 3.76 ± 0.073	4.331GlySer: 4.331 ± 0.076	4.897GlyThr: 4.897 ± 0.084	4.9GlyVal: 4.9 ± 0.075	1.033GlyTrp: 1.033 ± 0.036	3.354GlyTyr: 3.354 ± 0.074	0.0GlyXaa: 0.0 ± 0.0
His	1.222HisAla: 1.222 ± 0.036	0.307HisCys: 0.307 ± 0.016	0.99HisAsp: 0.99 ± 0.037	1.109HisGlu: 1.109 ± 0.033	0.767HisPhe: 0.767 ± 0.029	1.356HisGly: 1.356 ± 0.035	0.475HisHis: 0.475 ± 0.037	1.125HisIle: 1.125 ± 0.036	0.699HisLys: 0.699 ± 0.025	1.572HisLeu: 1.572 ± 0.046	0.533HisMet: 0.533 ± 0.026	0.646HisAsn: 0.646 ± 0.025	0.992HisPro: 0.992 ± 0.039	0.505HisGln: 0.505 ± 0.022	0.951HisArg: 0.951 ± 0.033	1.08HisSer: 1.08 ± 0.036	0.995HisThr: 0.995 ± 0.031	1.165HisVal: 1.165 ± 0.034	0.255HisTrp: 0.255 ± 0.016	0.75HisTyr: 0.75 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.533IleAla: 5.533 ± 0.094	1.144IleCys: 1.144 ± 0.036	3.543IleAsp: 3.543 ± 0.055	3.532IleGlu: 3.532 ± 0.063	2.002IlePhe: 2.002 ± 0.054	4.536IleGly: 4.536 ± 0.076	1.267IleHis: 1.267 ± 0.035	4.106IleIle: 4.106 ± 0.075	2.455IleLys: 2.455 ± 0.057	6.574IleLeu: 6.574 ± 0.09	1.592IleMet: 1.592 ± 0.041	2.304IleAsn: 2.304 ± 0.048	3.296IlePro: 3.296 ± 0.054	1.73IleGln: 1.73 ± 0.049	4.69IleArg: 4.69 ± 0.08	4.138IleSer: 4.138 ± 0.07	3.915IleThr: 3.915 ± 0.08	4.028IleVal: 4.028 ± 0.065	0.737IleTrp: 0.737 ± 0.027	1.946IleTyr: 1.946 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.05LysAla: 5.05 ± 0.072	0.57LysCys: 0.57 ± 0.026	3.387LysAsp: 3.387 ± 0.07	4.607LysGlu: 4.607 ± 0.075	1.196LysPhe: 1.196 ± 0.035	3.624LysGly: 3.624 ± 0.06	0.915LysHis: 0.915 ± 0.03	3.274LysIle: 3.274 ± 0.056	4.139LysLys: 4.139 ± 0.088	4.683LysLeu: 4.683 ± 0.073	1.661LysMet: 1.661 ± 0.043	2.451LysAsn: 2.451 ± 0.06	2.152LysPro: 2.152 ± 0.05	1.86LysGln: 1.86 ± 0.041	2.647LysArg: 2.647 ± 0.05	2.322LysSer: 2.322 ± 0.052	3.536LysThr: 3.536 ± 0.072	3.417LysVal: 3.417 ± 0.065	0.552LysTrp: 0.552 ± 0.025	2.095LysTyr: 2.095 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.653LeuAla: 7.653 ± 0.092	1.884LeuCys: 1.884 ± 0.046	5.308LeuAsp: 5.308 ± 0.08	5.78LeuGlu: 5.78 ± 0.093	3.911LeuPhe: 3.911 ± 0.075	5.879LeuGly: 5.879 ± 0.09	1.539LeuHis: 1.539 ± 0.041	5.749LeuIle: 5.749 ± 0.083	5.367LeuLys: 5.367 ± 0.077	9.627LeuLeu: 9.627 ± 0.15	2.745LeuMet: 2.745 ± 0.059	3.61LeuAsn: 3.61 ± 0.063	4.532LeuPro: 4.532 ± 0.082	2.721LeuGln: 2.721 ± 0.05	4.936LeuArg: 4.936 ± 0.091	6.067LeuSer: 6.067 ± 0.099	5.95LeuThr: 5.95 ± 0.087	5.549LeuVal: 5.549 ± 0.087	1.04LeuTrp: 1.04 ± 0.037	3.422LeuTyr: 3.422 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.423MetAla: 2.423 ± 0.051	0.312MetCys: 0.312 ± 0.018	1.907MetAsp: 1.907 ± 0.044	2.131MetGlu: 2.131 ± 0.049	0.893MetPhe: 0.893 ± 0.033	1.833MetGly: 1.833 ± 0.045	0.478MetHis: 0.478 ± 0.021	2.156MetIle: 2.156 ± 0.052	2.412MetLys: 2.412 ± 0.053	2.964MetLeu: 2.964 ± 0.058	1.01MetMet: 1.01 ± 0.031	1.561MetAsn: 1.561 ± 0.04	1.322MetPro: 1.322 ± 0.039	0.941MetGln: 0.941 ± 0.028	1.455MetArg: 1.455 ± 0.042	1.608MetSer: 1.608 ± 0.043	2.019MetThr: 2.019 ± 0.042	1.723MetVal: 1.723 ± 0.052	0.225MetTrp: 0.225 ± 0.014	0.818MetTyr: 0.818 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.296AsnAla: 3.296 ± 0.062	0.531AsnCys: 0.531 ± 0.027	2.085AsnAsp: 2.085 ± 0.044	2.585AsnGlu: 2.585 ± 0.056	1.134AsnPhe: 1.134 ± 0.031	3.77AsnGly: 3.77 ± 0.088	0.694AsnHis: 0.694 ± 0.026	2.305AsnIle: 2.305 ± 0.061	1.875AsnLys: 1.875 ± 0.049	3.335AsnLeu: 3.335 ± 0.062	1.155AsnMet: 1.155 ± 0.033	1.624AsnAsn: 1.624 ± 0.053	2.162AsnPro: 2.162 ± 0.049	1.128AsnGln: 1.128 ± 0.038	2.301AsnArg: 2.301 ± 0.048	1.948AsnSer: 1.948 ± 0.057	2.355AsnThr: 2.355 ± 0.053	2.509AsnVal: 2.509 ± 0.055	0.524AsnTrp: 0.524 ± 0.023	1.459AsnTyr: 1.459 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	4.037ProAla: 4.037 ± 0.084	0.563ProCys: 0.563 ± 0.025	3.489ProAsp: 3.489 ± 0.075	4.841ProGlu: 4.841 ± 0.088	1.713ProPhe: 1.713 ± 0.048	3.157ProGly: 3.157 ± 0.063	0.618ProHis: 0.618 ± 0.026	1.738ProIle: 1.738 ± 0.043	1.601ProLys: 1.601 ± 0.045	3.259ProLeu: 3.259 ± 0.058	1.097ProMet: 1.097 ± 0.032	1.17ProAsn: 1.17 ± 0.035	1.007ProPro: 1.007 ± 0.033	1.164ProGln: 1.164 ± 0.037	1.486ProArg: 1.486 ± 0.042	1.99ProSer: 1.99 ± 0.054	1.88ProThr: 1.88 ± 0.072	3.904ProVal: 3.904 ± 0.064	0.508ProTrp: 0.508 ± 0.022	1.475ProTyr: 1.475 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.633GlnAla: 2.633 ± 0.055	0.339GlnCys: 0.339 ± 0.019	1.625GlnAsp: 1.625 ± 0.046	2.303GlnGlu: 2.303 ± 0.054	1.101GlnPhe: 1.101 ± 0.032	1.993GlnGly: 1.993 ± 0.046	0.479GlnHis: 0.479 ± 0.02	1.968GlnIle: 1.968 ± 0.046	1.942GlnLys: 1.942 ± 0.051	2.515GlnLeu: 2.515 ± 0.05	1.112GlnMet: 1.112 ± 0.037	1.322GlnAsn: 1.322 ± 0.035	1.181GlnPro: 1.181 ± 0.041	1.157GlnGln: 1.157 ± 0.044	1.486GlnArg: 1.486 ± 0.044	1.371GlnSer: 1.371 ± 0.037	1.582GlnThr: 1.582 ± 0.041	1.951GlnVal: 1.951 ± 0.043	0.371GlnTrp: 0.371 ± 0.02	1.341GlnTyr: 1.341 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	3.476ArgAla: 3.476 ± 0.065	0.718ArgCys: 0.718 ± 0.033	3.034ArgAsp: 3.034 ± 0.057	4.576ArgGlu: 4.576 ± 0.081	2.328ArgPhe: 2.328 ± 0.05	3.01ArgGly: 3.01 ± 0.064	0.972ArgHis: 0.972 ± 0.035	4.014ArgIle: 4.014 ± 0.082	3.788ArgLys: 3.788 ± 0.074	4.98ArgLeu: 4.98 ± 0.095	1.801ArgMet: 1.801 ± 0.045	2.311ArgAsn: 2.311 ± 0.05	1.758ArgPro: 1.758 ± 0.051	1.866ArgGln: 1.866 ± 0.048	3.132ArgArg: 3.132 ± 0.067	2.611ArgSer: 2.611 ± 0.053	2.854ArgThr: 2.854 ± 0.055	3.291ArgVal: 3.291 ± 0.06	0.604ArgTrp: 0.604 ± 0.025	2.174ArgTyr: 2.174 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.751SerAla: 4.751 ± 0.072	0.861SerCys: 0.861 ± 0.031	3.112SerAsp: 3.112 ± 0.057	3.457SerGlu: 3.457 ± 0.061	2.766SerPhe: 2.766 ± 0.052	5.218SerGly: 5.218 ± 0.092	0.941SerHis: 0.941 ± 0.032	3.402SerIle: 3.402 ± 0.066	2.208SerLys: 2.208 ± 0.05	5.382SerLeu: 5.382 ± 0.08	1.709SerMet: 1.709 ± 0.04	1.741SerAsn: 1.741 ± 0.048	2.049SerPro: 2.049 ± 0.043	1.468SerGln: 1.468 ± 0.047	3.182SerArg: 3.182 ± 0.06	3.35SerSer: 3.35 ± 0.071	2.701SerThr: 2.701 ± 0.053	4.414SerVal: 4.414 ± 0.075	0.761SerTrp: 0.761 ± 0.028	2.051SerTyr: 2.051 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	5.503ThrAla: 5.503 ± 0.079	0.783ThrCys: 0.783 ± 0.028	3.77ThrAsp: 3.77 ± 0.073	4.586ThrGlu: 4.586 ± 0.098	2.21ThrPhe: 2.21 ± 0.052	5.427ThrGly: 5.427 ± 0.087	0.891ThrHis: 0.891 ± 0.033	3.578ThrIle: 3.578 ± 0.069	2.288ThrLys: 2.288 ± 0.058	5.533ThrLeu: 5.533 ± 0.075	1.427ThrMet: 1.427 ± 0.035	1.762ThrAsn: 1.762 ± 0.05	2.775ThrPro: 2.775 ± 0.072	1.346ThrGln: 1.346 ± 0.039	2.543ThrArg: 2.543 ± 0.047	2.722ThrSer: 2.722 ± 0.064	2.845ThrThr: 2.845 ± 0.062	5.463ThrVal: 5.463 ± 0.1	0.805ThrTrp: 0.805 ± 0.031	1.992ThrTyr: 1.992 ± 0.066	0.0ThrXaa: 0.0 ± 0.0
Val	4.402ValAla: 4.402 ± 0.07	1.233ValCys: 1.233 ± 0.038	3.479ValAsp: 3.479 ± 0.065	4.038ValGlu: 4.038 ± 0.072	3.14ValPhe: 3.14 ± 0.058	3.858ValGly: 3.858 ± 0.073	1.142ValHis: 1.142 ± 0.033	4.941ValIle: 4.941 ± 0.062	3.546ValLys: 3.546 ± 0.069	7.034ValLeu: 7.034 ± 0.109	2.042ValMet: 2.042 ± 0.044	2.778ValAsn: 2.778 ± 0.057	3.075ValPro: 3.075 ± 0.06	1.941ValGln: 1.941 ± 0.044	3.712ValArg: 3.712 ± 0.063	4.872ValSer: 4.872 ± 0.071	4.816ValThr: 4.816 ± 0.101	4.166ValVal: 4.166 ± 0.075	0.813ValTrp: 0.813 ± 0.032	2.668ValTyr: 2.668 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.814TrpAla: 0.814 ± 0.027	0.207TrpCys: 0.207 ± 0.016	0.778TrpAsp: 0.778 ± 0.029	0.824TrpGlu: 0.824 ± 0.031	0.531TrpPhe: 0.531 ± 0.023	0.794TrpGly: 0.794 ± 0.03	0.3TrpHis: 0.3 ± 0.022	0.819TrpIle: 0.819 ± 0.027	0.835TrpLys: 0.835 ± 0.028	1.255TrpLeu: 1.255 ± 0.042	0.391TrpMet: 0.391 ± 0.019	0.703TrpAsn: 0.703 ± 0.029	0.353TrpPro: 0.353 ± 0.021	0.503TrpGln: 0.503 ± 0.024	0.532TrpArg: 0.532 ± 0.025	0.621TrpSer: 0.621 ± 0.026	0.697TrpThr: 0.697 ± 0.033	0.67TrpVal: 0.67 ± 0.026	0.177TrpTrp: 0.177 ± 0.016	0.511TrpTyr: 0.511 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.051TyrAla: 3.051 ± 0.063	0.619TyrCys: 0.619 ± 0.023	2.603TyrAsp: 2.603 ± 0.077	2.522TyrGlu: 2.522 ± 0.053	1.54TyrPhe: 1.54 ± 0.044	2.868TyrGly: 2.868 ± 0.048	0.797TyrHis: 0.797 ± 0.028	2.246TyrIle: 2.246 ± 0.045	1.548TyrLys: 1.548 ± 0.039	3.406TyrLeu: 3.406 ± 0.054	0.945TyrMet: 0.945 ± 0.031	1.499TyrAsn: 1.499 ± 0.045	1.652TyrPro: 1.652 ± 0.045	1.205TyrGln: 1.205 ± 0.032	2.362TyrArg: 2.362 ± 0.05	2.158TyrSer: 2.158 ± 0.055	2.548TyrThr: 2.548 ± 0.089	2.381TyrVal: 2.381 ± 0.045	0.452TyrTrp: 0.452 ± 0.023	1.678TyrTyr: 1.678 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3011 proteins (1014911 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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