Amino acid dipepetide frequency for Flavobacterium phage FCL-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.799AlaAla: 1.799 ± 0.44
0.554AlaCys: 0.554 ± 0.189
3.529AlaAsp: 3.529 ± 0.466
3.39AlaGlu: 3.39 ± 0.585
2.422AlaPhe: 2.422 ± 0.397
3.598AlaGly: 3.598 ± 0.697
0.484AlaHis: 0.484 ± 0.196
4.705AlaIle: 4.705 ± 0.847
4.912AlaLys: 4.912 ± 0.698
3.114AlaLeu: 3.114 ± 0.654
0.83AlaMet: 0.83 ± 0.291
4.151AlaAsn: 4.151 ± 0.612
1.245AlaPro: 1.245 ± 0.323
1.73AlaGln: 1.73 ± 0.404
2.076AlaArg: 2.076 ± 0.339
4.29AlaSer: 4.29 ± 0.571
3.529AlaThr: 3.529 ± 0.672
2.698AlaVal: 2.698 ± 0.419
0.761AlaTrp: 0.761 ± 0.204
2.56AlaTyr: 2.56 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.346CysAla: 0.346 ± 0.145
0.138CysCys: 0.138 ± 0.11
0.692CysAsp: 0.692 ± 0.196
0.623CysGlu: 0.623 ± 0.187
0.415CysPhe: 0.415 ± 0.188
0.554CysGly: 0.554 ± 0.204
0.208CysHis: 0.208 ± 0.133
0.554CysIle: 0.554 ± 0.197
1.245CysLys: 1.245 ± 0.321
0.969CysLeu: 0.969 ± 0.338
0.277CysMet: 0.277 ± 0.131
0.554CysAsn: 0.554 ± 0.221
0.277CysPro: 0.277 ± 0.131
0.277CysGln: 0.277 ± 0.149
0.484CysArg: 0.484 ± 0.179
0.484CysSer: 0.484 ± 0.168
0.554CysThr: 0.554 ± 0.182
0.484CysVal: 0.484 ± 0.214
0.0CysTrp: 0.0 ± 0.0
0.277CysTyr: 0.277 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
4.221AspAla: 4.221 ± 0.503
0.415AspCys: 0.415 ± 0.166
3.183AspAsp: 3.183 ± 0.47
3.805AspGlu: 3.805 ± 0.408
3.944AspPhe: 3.944 ± 0.449
2.837AspGly: 2.837 ± 0.344
0.554AspHis: 0.554 ± 0.181
4.636AspIle: 4.636 ± 0.554
4.082AspLys: 4.082 ± 0.562
6.781AspLeu: 6.781 ± 0.65
0.899AspMet: 0.899 ± 0.283
4.428AspAsn: 4.428 ± 0.518
1.661AspPro: 1.661 ± 0.329
1.245AspGln: 1.245 ± 0.316
1.661AspArg: 1.661 ± 0.399
3.39AspSer: 3.39 ± 0.578
2.629AspThr: 2.629 ± 0.498
4.013AspVal: 4.013 ± 0.52
0.969AspTrp: 0.969 ± 0.286
2.906AspTyr: 2.906 ± 0.522
0.0AspXaa: 0.0 ± 0.0
Glu
3.875GluAla: 3.875 ± 0.509
0.484GluCys: 0.484 ± 0.181
2.768GluAsp: 2.768 ± 0.448
4.982GluGlu: 4.982 ± 0.786
3.944GluPhe: 3.944 ± 0.526
1.937GluGly: 1.937 ± 0.416
1.038GluHis: 1.038 ± 0.253
5.95GluIle: 5.95 ± 0.804
7.542GluLys: 7.542 ± 0.925
7.818GluLeu: 7.818 ± 0.744
2.698GluMet: 2.698 ± 0.424
5.812GluAsn: 5.812 ± 0.811
1.522GluPro: 1.522 ± 0.361
2.422GluGln: 2.422 ± 0.421
3.598GluArg: 3.598 ± 0.68
4.843GluSer: 4.843 ± 0.535
3.598GluThr: 3.598 ± 0.602
3.667GluVal: 3.667 ± 0.387
0.761GluTrp: 0.761 ± 0.214
3.044GluTyr: 3.044 ± 0.478
0.0GluXaa: 0.0 ± 0.0
Phe
1.937PheAla: 1.937 ± 0.456
0.484PheCys: 0.484 ± 0.199
3.875PheAsp: 3.875 ± 0.434
4.082PheGlu: 4.082 ± 0.65
1.522PhePhe: 1.522 ± 0.321
3.39PheGly: 3.39 ± 0.553
0.484PheHis: 0.484 ± 0.185
3.39PheIle: 3.39 ± 0.471
4.705PheLys: 4.705 ± 0.572
3.736PheLeu: 3.736 ± 0.505
1.591PheMet: 1.591 ± 0.281
3.39PheAsn: 3.39 ± 0.503
1.176PhePro: 1.176 ± 0.228
1.384PheGln: 1.384 ± 0.267
1.453PheArg: 1.453 ± 0.303
2.629PheSer: 2.629 ± 0.456
2.975PheThr: 2.975 ± 0.498
2.422PheVal: 2.422 ± 0.45
0.623PheTrp: 0.623 ± 0.194
2.007PheTyr: 2.007 ± 0.39
0.0PheXaa: 0.0 ± 0.0
Gly
3.044GlyAla: 3.044 ± 0.617
0.484GlyCys: 0.484 ± 0.153
3.875GlyAsp: 3.875 ± 0.571
3.183GlyGlu: 3.183 ± 0.407
3.114GlyPhe: 3.114 ± 0.494
4.428GlyGly: 4.428 ± 0.709
0.484GlyHis: 0.484 ± 0.216
4.221GlyIle: 4.221 ± 0.463
5.258GlyLys: 5.258 ± 0.661
4.705GlyLeu: 4.705 ± 0.516
0.83GlyMet: 0.83 ± 0.228
3.114GlyAsn: 3.114 ± 0.441
0.415GlyPro: 0.415 ± 0.151
1.661GlyGln: 1.661 ± 0.318
1.73GlyArg: 1.73 ± 0.39
4.082GlySer: 4.082 ± 0.618
3.183GlyThr: 3.183 ± 0.512
4.151GlyVal: 4.151 ± 0.593
0.692GlyTrp: 0.692 ± 0.199
2.56GlyTyr: 2.56 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
0.484HisAla: 0.484 ± 0.21
0.277HisCys: 0.277 ± 0.167
0.761HisAsp: 0.761 ± 0.219
1.176HisGlu: 1.176 ± 0.28
0.761HisPhe: 0.761 ± 0.207
1.107HisGly: 1.107 ± 0.386
0.138HisHis: 0.138 ± 0.096
1.038HisIle: 1.038 ± 0.275
1.107HisLys: 1.107 ± 0.337
0.969HisLeu: 0.969 ± 0.282
0.069HisMet: 0.069 ± 0.08
1.038HisAsn: 1.038 ± 0.301
0.761HisPro: 0.761 ± 0.184
0.554HisGln: 0.554 ± 0.193
0.761HisArg: 0.761 ± 0.202
0.484HisSer: 0.484 ± 0.181
0.277HisThr: 0.277 ± 0.115
0.554HisVal: 0.554 ± 0.226
0.415HisTrp: 0.415 ± 0.181
0.623HisTyr: 0.623 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
4.912IleAla: 4.912 ± 0.705
0.554IleCys: 0.554 ± 0.242
6.365IleAsp: 6.365 ± 0.69
6.02IleGlu: 6.02 ± 0.838
2.975IlePhe: 2.975 ± 0.509
4.497IleGly: 4.497 ± 0.679
1.176IleHis: 1.176 ± 0.259
6.02IleIle: 6.02 ± 0.629
7.542IleLys: 7.542 ± 0.915
6.089IleLeu: 6.089 ± 0.614
1.453IleMet: 1.453 ± 0.289
4.912IleAsn: 4.912 ± 0.607
2.698IlePro: 2.698 ± 0.415
3.875IleGln: 3.875 ± 0.539
2.698IleArg: 2.698 ± 0.406
5.189IleSer: 5.189 ± 0.751
5.12IleThr: 5.12 ± 0.584
4.29IleVal: 4.29 ± 0.424
0.554IleTrp: 0.554 ± 0.231
2.975IleTyr: 2.975 ± 0.381
0.0IleXaa: 0.0 ± 0.0
Lys
5.12LysAla: 5.12 ± 0.611
1.107LysCys: 1.107 ± 0.364
5.258LysAsp: 5.258 ± 0.607
6.988LysGlu: 6.988 ± 0.961
3.114LysPhe: 3.114 ± 0.482
5.397LysGly: 5.397 ± 0.588
1.384LysHis: 1.384 ± 0.362
6.642LysIle: 6.642 ± 0.65
8.026LysLys: 8.026 ± 0.869
8.58LysLeu: 8.58 ± 0.803
2.698LysMet: 2.698 ± 0.44
6.296LysAsn: 6.296 ± 0.69
3.805LysPro: 3.805 ± 0.54
3.736LysGln: 3.736 ± 0.507
3.944LysArg: 3.944 ± 0.628
5.328LysSer: 5.328 ± 0.68
5.258LysThr: 5.258 ± 0.554
4.567LysVal: 4.567 ± 0.733
1.176LysTrp: 1.176 ± 0.234
3.598LysTyr: 3.598 ± 0.595
0.0LysXaa: 0.0 ± 0.0
Leu
4.774LeuAla: 4.774 ± 0.578
0.554LeuCys: 0.554 ± 0.19
3.875LeuAsp: 3.875 ± 0.482
8.026LeuGlu: 8.026 ± 0.776
4.29LeuPhe: 4.29 ± 0.499
5.12LeuGly: 5.12 ± 0.66
0.761LeuHis: 0.761 ± 0.216
7.68LeuIle: 7.68 ± 0.79
8.164LeuLys: 8.164 ± 0.656
6.227LeuLeu: 6.227 ± 0.604
2.076LeuMet: 2.076 ± 0.461
6.158LeuAsn: 6.158 ± 0.63
2.698LeuPro: 2.698 ± 0.404
2.768LeuGln: 2.768 ± 0.391
3.39LeuArg: 3.39 ± 0.423
5.12LeuSer: 5.12 ± 0.694
6.089LeuThr: 6.089 ± 0.704
5.12LeuVal: 5.12 ± 0.506
0.899LeuTrp: 0.899 ± 0.293
2.768LeuTyr: 2.768 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
2.076MetAla: 2.076 ± 0.509
0.208MetCys: 0.208 ± 0.127
0.83MetAsp: 0.83 ± 0.235
1.107MetGlu: 1.107 ± 0.271
0.623MetPhe: 0.623 ± 0.233
1.176MetGly: 1.176 ± 0.241
0.138MetHis: 0.138 ± 0.096
2.214MetIle: 2.214 ± 0.411
2.629MetLys: 2.629 ± 0.419
1.245MetLeu: 1.245 ± 0.329
0.069MetMet: 0.069 ± 0.061
1.384MetAsn: 1.384 ± 0.319
0.761MetPro: 0.761 ± 0.252
1.384MetGln: 1.384 ± 0.303
1.176MetArg: 1.176 ± 0.293
1.661MetSer: 1.661 ± 0.397
1.661MetThr: 1.661 ± 0.355
0.899MetVal: 0.899 ± 0.241
0.208MetTrp: 0.208 ± 0.16
0.899MetTyr: 0.899 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.114AsnAla: 3.114 ± 0.478
0.623AsnCys: 0.623 ± 0.194
4.359AsnAsp: 4.359 ± 0.582
6.089AsnGlu: 6.089 ± 0.563
2.906AsnPhe: 2.906 ± 0.41
4.705AsnGly: 4.705 ± 0.69
1.107AsnHis: 1.107 ± 0.277
5.397AsnIle: 5.397 ± 0.635
6.296AsnLys: 6.296 ± 0.648
6.711AsnLeu: 6.711 ± 0.58
1.315AsnMet: 1.315 ± 0.251
6.642AsnAsn: 6.642 ± 0.713
2.56AsnPro: 2.56 ± 0.437
2.56AsnGln: 2.56 ± 0.433
2.352AsnArg: 2.352 ± 0.338
4.636AsnSer: 4.636 ± 0.612
4.359AsnThr: 4.359 ± 0.564
3.598AsnVal: 3.598 ± 0.593
0.346AsnTrp: 0.346 ± 0.149
3.252AsnTyr: 3.252 ± 0.549
0.0AsnXaa: 0.0 ± 0.0
Pro
1.176ProAla: 1.176 ± 0.363
0.415ProCys: 0.415 ± 0.158
2.56ProAsp: 2.56 ± 0.506
1.868ProGlu: 1.868 ± 0.391
1.661ProPhe: 1.661 ± 0.345
0.554ProGly: 0.554 ± 0.214
0.692ProHis: 0.692 ± 0.18
2.56ProIle: 2.56 ± 0.398
2.768ProLys: 2.768 ± 0.488
2.283ProLeu: 2.283 ± 0.382
0.277ProMet: 0.277 ± 0.116
2.214ProAsn: 2.214 ± 0.352
0.346ProPro: 0.346 ± 0.135
1.315ProGln: 1.315 ± 0.425
0.692ProArg: 0.692 ± 0.215
2.007ProSer: 2.007 ± 0.387
2.422ProThr: 2.422 ± 0.463
1.937ProVal: 1.937 ± 0.448
0.138ProTrp: 0.138 ± 0.098
1.384ProTyr: 1.384 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
1.73GlnAla: 1.73 ± 0.299
0.208GlnCys: 0.208 ± 0.114
1.522GlnAsp: 1.522 ± 0.297
2.906GlnGlu: 2.906 ± 0.499
1.384GlnPhe: 1.384 ± 0.286
1.868GlnGly: 1.868 ± 0.337
0.83GlnHis: 0.83 ± 0.362
2.698GlnIle: 2.698 ± 0.364
2.837GlnLys: 2.837 ± 0.558
3.944GlnLeu: 3.944 ± 0.457
1.384GlnMet: 1.384 ± 0.319
2.283GlnAsn: 2.283 ± 0.456
1.107GlnPro: 1.107 ± 0.389
2.145GlnGln: 2.145 ± 0.516
1.453GlnArg: 1.453 ± 0.276
2.768GlnSer: 2.768 ± 0.41
1.868GlnThr: 1.868 ± 0.342
1.868GlnVal: 1.868 ± 0.35
0.277GlnTrp: 0.277 ± 0.17
1.591GlnTyr: 1.591 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
1.73ArgAla: 1.73 ± 0.301
0.346ArgCys: 0.346 ± 0.144
1.868ArgAsp: 1.868 ± 0.449
2.975ArgGlu: 2.975 ± 0.533
2.837ArgPhe: 2.837 ± 0.53
1.453ArgGly: 1.453 ± 0.253
0.554ArgHis: 0.554 ± 0.191
3.114ArgIle: 3.114 ± 0.391
3.875ArgLys: 3.875 ± 0.658
3.736ArgLeu: 3.736 ± 0.707
0.83ArgMet: 0.83 ± 0.225
2.145ArgAsn: 2.145 ± 0.373
0.623ArgPro: 0.623 ± 0.176
1.315ArgGln: 1.315 ± 0.296
1.107ArgArg: 1.107 ± 0.304
1.661ArgSer: 1.661 ± 0.376
2.076ArgThr: 2.076 ± 0.338
2.837ArgVal: 2.837 ± 0.432
0.277ArgTrp: 0.277 ± 0.162
1.868ArgTyr: 1.868 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
3.252SerAla: 3.252 ± 0.796
0.484SerCys: 0.484 ± 0.173
3.459SerAsp: 3.459 ± 0.386
4.497SerGlu: 4.497 ± 0.629
3.252SerPhe: 3.252 ± 0.468
4.221SerGly: 4.221 ± 0.514
0.761SerHis: 0.761 ± 0.274
5.397SerIle: 5.397 ± 0.565
6.158SerLys: 6.158 ± 0.756
5.604SerLeu: 5.604 ± 0.721
1.384SerMet: 1.384 ± 0.298
4.774SerAsn: 4.774 ± 0.61
1.107SerPro: 1.107 ± 0.246
2.56SerGln: 2.56 ± 0.417
2.214SerArg: 2.214 ± 0.379
2.629SerSer: 2.629 ± 0.491
3.598SerThr: 3.598 ± 0.452
4.29SerVal: 4.29 ± 0.515
0.899SerTrp: 0.899 ± 0.228
2.837SerTyr: 2.837 ± 0.49
0.0SerXaa: 0.0 ± 0.0
Thr
3.598ThrAla: 3.598 ± 0.618
0.484ThrCys: 0.484 ± 0.249
3.805ThrAsp: 3.805 ± 0.418
3.598ThrGlu: 3.598 ± 0.533
2.629ThrPhe: 2.629 ± 0.447
3.529ThrGly: 3.529 ± 0.518
0.83ThrHis: 0.83 ± 0.287
5.189ThrIle: 5.189 ± 0.514
4.843ThrLys: 4.843 ± 0.731
4.636ThrLeu: 4.636 ± 0.657
1.384ThrMet: 1.384 ± 0.345
4.636ThrAsn: 4.636 ± 0.706
2.698ThrPro: 2.698 ± 0.453
1.591ThrGln: 1.591 ± 0.352
2.076ThrArg: 2.076 ± 0.39
3.39ThrSer: 3.39 ± 0.531
3.598ThrThr: 3.598 ± 0.52
4.359ThrVal: 4.359 ± 0.628
0.692ThrTrp: 0.692 ± 0.159
2.214ThrTyr: 2.214 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
2.906ValAla: 2.906 ± 0.555
0.899ValCys: 0.899 ± 0.252
2.768ValAsp: 2.768 ± 0.44
4.013ValGlu: 4.013 ± 0.476
2.56ValPhe: 2.56 ± 0.368
2.145ValGly: 2.145 ± 0.373
0.899ValHis: 0.899 ± 0.268
5.051ValIle: 5.051 ± 0.572
5.051ValLys: 5.051 ± 0.655
4.497ValLeu: 4.497 ± 0.525
0.969ValMet: 0.969 ± 0.261
5.189ValAsn: 5.189 ± 0.659
1.591ValPro: 1.591 ± 0.328
1.73ValGln: 1.73 ± 0.327
2.629ValArg: 2.629 ± 0.458
4.705ValSer: 4.705 ± 0.493
3.667ValThr: 3.667 ± 0.556
3.39ValVal: 3.39 ± 0.481
0.415ValTrp: 0.415 ± 0.173
3.114ValTyr: 3.114 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
0.83TrpAla: 0.83 ± 0.238
0.138TrpCys: 0.138 ± 0.09
0.484TrpAsp: 0.484 ± 0.193
0.554TrpGlu: 0.554 ± 0.182
0.623TrpPhe: 0.623 ± 0.214
0.761TrpGly: 0.761 ± 0.215
0.277TrpHis: 0.277 ± 0.133
0.969TrpIle: 0.969 ± 0.286
1.107TrpLys: 1.107 ± 0.276
0.554TrpLeu: 0.554 ± 0.206
0.346TrpMet: 0.346 ± 0.146
0.623TrpAsn: 0.623 ± 0.161
0.138TrpPro: 0.138 ± 0.101
0.346TrpGln: 0.346 ± 0.191
0.277TrpArg: 0.277 ± 0.171
0.692TrpSer: 0.692 ± 0.27
0.761TrpThr: 0.761 ± 0.21
0.554TrpVal: 0.554 ± 0.2
0.138TrpTrp: 0.138 ± 0.081
0.554TrpTyr: 0.554 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.453TyrAla: 1.453 ± 0.335
0.554TyrCys: 0.554 ± 0.188
2.422TyrAsp: 2.422 ± 0.374
2.422TyrGlu: 2.422 ± 0.342
2.283TyrPhe: 2.283 ± 0.369
1.937TyrGly: 1.937 ± 0.383
0.554TyrHis: 0.554 ± 0.163
2.491TyrIle: 2.491 ± 0.356
3.875TyrLys: 3.875 ± 0.462
4.359TyrLeu: 4.359 ± 0.485
0.899TyrMet: 0.899 ± 0.29
3.183TyrAsn: 3.183 ± 0.605
2.076TyrPro: 2.076 ± 0.353
2.076TyrGln: 2.076 ± 0.385
1.453TyrArg: 1.453 ± 0.327
3.39TyrSer: 3.39 ± 0.509
2.56TyrThr: 2.56 ± 0.401
2.56TyrVal: 2.56 ± 0.431
0.484TyrTrp: 0.484 ± 0.163
1.799TyrTyr: 1.799 ± 0.379
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14454 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski