Salmonella phage 35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chivirus; Salmonella virus 35

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7CFH9|A0A0N7CFH9_9CAUD Uncharacterized protein OS=Salmonella phage 35 OX=1654888 GN=SP35_8 PE=4 SV=1
MM1 pKa = 7.41AGLWFFVVTMCGAVGADD18 pKa = 4.02NMAADD23 pKa = 3.87CHH25 pKa = 7.17DD26 pKa = 3.9YY27 pKa = 11.38VIDD30 pKa = 4.68AGISYY35 pKa = 10.1DD36 pKa = 3.54DD37 pKa = 4.13CRR39 pKa = 11.84AAVAAYY45 pKa = 8.14PGRR48 pKa = 11.84IGLYY52 pKa = 8.41SARR55 pKa = 11.84CDD57 pKa = 2.91RR58 pKa = 11.84GEE60 pKa = 3.96VVEE63 pKa = 4.71GGNHH67 pKa = 4.72EE68 pKa = 4.2

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N6WGD7|A0A0N6WGD7_9CAUD Putative tail component K-like protein OS=Salmonella phage 35 OX=1654888 GN=SP35_86 PE=4 SV=1
MM1 pKa = 7.61LFEE4 pKa = 5.51CIAKK8 pKa = 10.18RR9 pKa = 11.84SFRR12 pKa = 11.84EE13 pKa = 3.52SRR15 pKa = 11.84YY16 pKa = 9.85GLFVNVRR23 pKa = 11.84QVQDD27 pKa = 3.33QAVQRR32 pKa = 11.84LDD34 pKa = 3.63LLVTFVGRR42 pKa = 11.84VVQRR46 pKa = 11.84ARR48 pKa = 11.84CKK50 pKa = 10.47IMAFSS55 pKa = 3.56

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16922

41

778

186.0

20.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.715 ± 0.469

1.04 ± 0.102

6.63 ± 0.264

5.898 ± 0.306

3.41 ± 0.158

7.121 ± 0.261

1.672 ± 0.137

5.372 ± 0.203

4.946 ± 0.256

7.369 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.156 ± 0.147

4.107 ± 0.178

4.881 ± 0.345

3.841 ± 0.302

6.536 ± 0.239

5.649 ± 0.222

6.441 ± 0.322

7.02 ± 0.21

1.696 ± 0.128

3.498 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski