Amino acid dipepetide frequency for Salmonella phage 35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.115AlaAla: 12.115 ± 1.306
0.827AlaCys: 0.827 ± 0.255
6.914AlaAsp: 6.914 ± 0.665
5.969AlaGlu: 5.969 ± 0.686
2.837AlaPhe: 2.837 ± 0.292
8.096AlaGly: 8.096 ± 0.56
1.3AlaHis: 1.3 ± 0.248
4.669AlaIle: 4.669 ± 0.531
5.26AlaLys: 5.26 ± 0.811
7.446AlaLeu: 7.446 ± 0.688
3.841AlaMet: 3.841 ± 0.486
3.605AlaAsn: 3.605 ± 0.418
3.369AlaPro: 3.369 ± 0.487
3.782AlaGln: 3.782 ± 0.598
5.792AlaArg: 5.792 ± 0.758
5.142AlaSer: 5.142 ± 0.684
5.851AlaThr: 5.851 ± 0.794
6.205AlaVal: 6.205 ± 0.678
1.123AlaTrp: 1.123 ± 0.263
3.014AlaTyr: 3.014 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.532CysAla: 0.532 ± 0.169
0.295CysCys: 0.295 ± 0.123
0.946CysAsp: 0.946 ± 0.25
0.827CysGlu: 0.827 ± 0.239
0.295CysPhe: 0.295 ± 0.125
0.768CysGly: 0.768 ± 0.207
0.295CysHis: 0.295 ± 0.119
0.532CysIle: 0.532 ± 0.14
0.295CysLys: 0.295 ± 0.115
0.768CysLeu: 0.768 ± 0.235
0.295CysMet: 0.295 ± 0.122
0.236CysAsn: 0.236 ± 0.11
0.886CysPro: 0.886 ± 0.271
0.177CysGln: 0.177 ± 0.09
0.65CysArg: 0.65 ± 0.191
0.591CysSer: 0.591 ± 0.174
0.827CysThr: 0.827 ± 0.223
0.591CysVal: 0.591 ± 0.204
0.236CysTrp: 0.236 ± 0.108
0.355CysTyr: 0.355 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.501AspAla: 6.501 ± 0.566
0.532AspCys: 0.532 ± 0.196
4.669AspAsp: 4.669 ± 0.571
5.201AspGlu: 5.201 ± 0.708
2.6AspPhe: 2.6 ± 0.38
5.555AspGly: 5.555 ± 0.614
1.3AspHis: 1.3 ± 0.27
3.132AspIle: 3.132 ± 0.411
3.191AspLys: 3.191 ± 0.516
5.496AspLeu: 5.496 ± 0.688
2.246AspMet: 2.246 ± 0.346
2.305AspAsn: 2.305 ± 0.416
3.723AspPro: 3.723 ± 0.611
2.128AspGln: 2.128 ± 0.389
3.487AspArg: 3.487 ± 0.384
1.95AspSer: 1.95 ± 0.353
3.546AspThr: 3.546 ± 0.526
5.26AspVal: 5.26 ± 0.548
1.655AspTrp: 1.655 ± 0.31
1.832AspTyr: 1.832 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
5.555GluAla: 5.555 ± 0.659
0.532GluCys: 0.532 ± 0.164
4.019GluAsp: 4.019 ± 0.544
3.309GluGlu: 3.309 ± 0.643
2.009GluPhe: 2.009 ± 0.446
4.137GluGly: 4.137 ± 0.555
1.123GluHis: 1.123 ± 0.256
3.782GluIle: 3.782 ± 0.524
2.246GluLys: 2.246 ± 0.367
4.078GluLeu: 4.078 ± 0.496
1.95GluMet: 1.95 ± 0.31
2.482GluAsn: 2.482 ± 0.435
2.541GluPro: 2.541 ± 0.463
2.423GluGln: 2.423 ± 0.329
3.9GluArg: 3.9 ± 0.576
3.546GluSer: 3.546 ± 0.461
2.719GluThr: 2.719 ± 0.399
4.728GluVal: 4.728 ± 0.518
1.596GluTrp: 1.596 ± 0.236
2.305GluTyr: 2.305 ± 0.414
0.0GluXaa: 0.0 ± 0.0
Phe
3.25PheAla: 3.25 ± 0.426
0.473PheCys: 0.473 ± 0.136
2.896PheAsp: 2.896 ± 0.379
1.891PheGlu: 1.891 ± 0.397
1.005PhePhe: 1.005 ± 0.263
2.659PheGly: 2.659 ± 0.392
0.709PheHis: 0.709 ± 0.189
2.068PheIle: 2.068 ± 0.317
1.832PheLys: 1.832 ± 0.446
1.95PheLeu: 1.95 ± 0.312
1.005PheMet: 1.005 ± 0.262
1.891PheAsn: 1.891 ± 0.362
1.3PhePro: 1.3 ± 0.277
1.123PheGln: 1.123 ± 0.266
2.837PheArg: 2.837 ± 0.395
1.832PheSer: 1.832 ± 0.285
2.364PheThr: 2.364 ± 0.409
1.832PheVal: 1.832 ± 0.395
0.236PheTrp: 0.236 ± 0.16
0.946PheTyr: 0.946 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
5.969GlyAla: 5.969 ± 0.669
1.064GlyCys: 1.064 ± 0.292
3.96GlyAsp: 3.96 ± 0.569
4.964GlyGlu: 4.964 ± 0.595
2.364GlyPhe: 2.364 ± 0.417
6.087GlyGly: 6.087 ± 0.609
1.005GlyHis: 1.005 ± 0.254
3.9GlyIle: 3.9 ± 0.529
3.25GlyLys: 3.25 ± 0.52
6.383GlyLeu: 6.383 ± 0.712
1.655GlyMet: 1.655 ± 0.313
2.6GlyAsn: 2.6 ± 0.386
1.773GlyPro: 1.773 ± 0.332
3.073GlyGln: 3.073 ± 0.47
5.023GlyArg: 5.023 ± 0.563
3.546GlySer: 3.546 ± 0.483
5.082GlyThr: 5.082 ± 0.871
4.669GlyVal: 4.669 ± 0.394
1.418GlyTrp: 1.418 ± 0.333
3.428GlyTyr: 3.428 ± 0.58
0.0GlyXaa: 0.0 ± 0.0
His
1.714HisAla: 1.714 ± 0.319
0.118HisCys: 0.118 ± 0.091
1.418HisAsp: 1.418 ± 0.328
0.827HisGlu: 0.827 ± 0.199
0.532HisPhe: 0.532 ± 0.199
1.418HisGly: 1.418 ± 0.234
0.414HisHis: 0.414 ± 0.159
1.241HisIle: 1.241 ± 0.272
0.65HisLys: 0.65 ± 0.192
1.3HisLeu: 1.3 ± 0.24
0.295HisMet: 0.295 ± 0.139
0.709HisAsn: 0.709 ± 0.189
0.886HisPro: 0.886 ± 0.202
0.532HisGln: 0.532 ± 0.182
1.241HisArg: 1.241 ± 0.213
0.591HisSer: 0.591 ± 0.179
0.591HisThr: 0.591 ± 0.174
1.005HisVal: 1.005 ± 0.257
0.414HisTrp: 0.414 ± 0.152
0.827HisTyr: 0.827 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
5.082IleAla: 5.082 ± 0.568
0.591IleCys: 0.591 ± 0.17
3.664IleAsp: 3.664 ± 0.375
4.196IleGlu: 4.196 ± 0.525
1.477IlePhe: 1.477 ± 0.322
4.314IleGly: 4.314 ± 0.559
1.359IleHis: 1.359 ± 0.369
2.6IleIle: 2.6 ± 0.397
3.191IleLys: 3.191 ± 0.559
2.837IleLeu: 2.837 ± 0.443
1.596IleMet: 1.596 ± 0.287
2.364IleAsn: 2.364 ± 0.37
2.187IlePro: 2.187 ± 0.379
1.655IleGln: 1.655 ± 0.311
3.723IleArg: 3.723 ± 0.448
2.955IleSer: 2.955 ± 0.456
3.428IleThr: 3.428 ± 0.479
4.019IleVal: 4.019 ± 0.479
0.768IleTrp: 0.768 ± 0.255
1.714IleTyr: 1.714 ± 0.341
0.0IleXaa: 0.0 ± 0.0
Lys
5.023LysAla: 5.023 ± 0.568
0.473LysCys: 0.473 ± 0.161
3.073LysAsp: 3.073 ± 0.533
2.837LysGlu: 2.837 ± 0.413
1.596LysPhe: 1.596 ± 0.322
2.896LysGly: 2.896 ± 0.416
1.005LysHis: 1.005 ± 0.222
2.6LysIle: 2.6 ± 0.402
2.659LysLys: 2.659 ± 0.413
3.25LysLeu: 3.25 ± 0.444
1.95LysMet: 1.95 ± 0.319
1.182LysAsn: 1.182 ± 0.248
2.837LysPro: 2.837 ± 0.409
1.596LysGln: 1.596 ± 0.32
3.664LysArg: 3.664 ± 0.487
2.423LysSer: 2.423 ± 0.335
3.546LysThr: 3.546 ± 0.42
3.487LysVal: 3.487 ± 0.412
1.064LysTrp: 1.064 ± 0.228
2.305LysTyr: 2.305 ± 0.377
0.0LysXaa: 0.0 ± 0.0
Leu
6.855LeuAla: 6.855 ± 0.822
0.946LeuCys: 0.946 ± 0.248
5.142LeuAsp: 5.142 ± 0.668
4.432LeuGlu: 4.432 ± 0.47
2.482LeuPhe: 2.482 ± 0.456
3.487LeuGly: 3.487 ± 0.488
1.182LeuHis: 1.182 ± 0.242
3.664LeuIle: 3.664 ± 0.49
3.9LeuLys: 3.9 ± 0.463
4.196LeuLeu: 4.196 ± 0.576
2.009LeuMet: 2.009 ± 0.45
3.9LeuAsn: 3.9 ± 0.466
4.61LeuPro: 4.61 ± 0.575
2.305LeuGln: 2.305 ± 0.511
4.964LeuArg: 4.964 ± 0.552
5.673LeuSer: 5.673 ± 0.763
5.614LeuThr: 5.614 ± 0.656
4.61LeuVal: 4.61 ± 0.479
1.064LeuTrp: 1.064 ± 0.223
2.6LeuTyr: 2.6 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
3.428MetAla: 3.428 ± 0.47
0.355MetCys: 0.355 ± 0.128
1.832MetAsp: 1.832 ± 0.265
1.359MetGlu: 1.359 ± 0.271
0.946MetPhe: 0.946 ± 0.234
1.832MetGly: 1.832 ± 0.354
0.414MetHis: 0.414 ± 0.155
1.359MetIle: 1.359 ± 0.292
2.128MetLys: 2.128 ± 0.362
2.246MetLeu: 2.246 ± 0.351
0.591MetMet: 0.591 ± 0.174
1.596MetAsn: 1.596 ± 0.294
1.123MetPro: 1.123 ± 0.218
1.123MetGln: 1.123 ± 0.295
2.364MetArg: 2.364 ± 0.367
2.246MetSer: 2.246 ± 0.384
2.482MetThr: 2.482 ± 0.374
2.423MetVal: 2.423 ± 0.364
0.414MetTrp: 0.414 ± 0.141
1.3MetTyr: 1.3 ± 0.278
0.0MetXaa: 0.0 ± 0.0
Asn
4.551AsnAla: 4.551 ± 0.639
0.236AsnCys: 0.236 ± 0.104
2.482AsnAsp: 2.482 ± 0.376
1.714AsnGlu: 1.714 ± 0.309
1.359AsnPhe: 1.359 ± 0.223
3.191AsnGly: 3.191 ± 0.425
0.65AsnHis: 0.65 ± 0.236
2.187AsnIle: 2.187 ± 0.339
1.596AsnLys: 1.596 ± 0.331
2.778AsnLeu: 2.778 ± 0.425
1.123AsnMet: 1.123 ± 0.26
1.537AsnAsn: 1.537 ± 0.422
3.487AsnPro: 3.487 ± 0.409
1.773AsnGln: 1.773 ± 0.362
2.187AsnArg: 2.187 ± 0.347
2.719AsnSer: 2.719 ± 0.47
2.364AsnThr: 2.364 ± 0.429
3.014AsnVal: 3.014 ± 0.365
0.532AsnTrp: 0.532 ± 0.159
1.596AsnTyr: 1.596 ± 0.318
0.0AsnXaa: 0.0 ± 0.0
Pro
3.9ProAla: 3.9 ± 0.463
0.414ProCys: 0.414 ± 0.156
3.546ProAsp: 3.546 ± 0.595
2.955ProGlu: 2.955 ± 0.397
1.773ProPhe: 1.773 ± 0.298
3.428ProGly: 3.428 ± 0.517
0.768ProHis: 0.768 ± 0.181
2.482ProIle: 2.482 ± 0.404
2.896ProLys: 2.896 ± 0.509
3.9ProLeu: 3.9 ± 0.574
1.655ProMet: 1.655 ± 0.342
2.009ProAsn: 2.009 ± 0.322
3.014ProPro: 3.014 ± 0.701
1.241ProGln: 1.241 ± 0.271
2.364ProArg: 2.364 ± 0.437
3.25ProSer: 3.25 ± 0.487
3.014ProThr: 3.014 ± 0.443
3.428ProVal: 3.428 ± 0.482
0.65ProTrp: 0.65 ± 0.227
2.128ProTyr: 2.128 ± 0.358
0.0ProXaa: 0.0 ± 0.0
Gln
3.782GlnAla: 3.782 ± 0.798
0.177GlnCys: 0.177 ± 0.096
2.187GlnAsp: 2.187 ± 0.365
1.655GlnGlu: 1.655 ± 0.266
2.364GlnPhe: 2.364 ± 0.442
1.537GlnGly: 1.537 ± 0.335
0.827GlnHis: 0.827 ± 0.256
2.482GlnIle: 2.482 ± 0.311
2.423GlnLys: 2.423 ± 0.401
2.305GlnLeu: 2.305 ± 0.385
1.832GlnMet: 1.832 ± 0.363
1.596GlnAsn: 1.596 ± 0.274
1.773GlnPro: 1.773 ± 0.382
1.832GlnGln: 1.832 ± 0.553
2.719GlnArg: 2.719 ± 0.433
2.068GlnSer: 2.068 ± 0.346
2.068GlnThr: 2.068 ± 0.367
2.719GlnVal: 2.719 ± 0.581
0.65GlnTrp: 0.65 ± 0.174
1.418GlnTyr: 1.418 ± 0.274
0.0GlnXaa: 0.0 ± 0.0
Arg
6.619ArgAla: 6.619 ± 0.727
0.886ArgCys: 0.886 ± 0.24
4.078ArgAsp: 4.078 ± 0.471
3.369ArgGlu: 3.369 ± 0.706
2.423ArgPhe: 2.423 ± 0.413
3.723ArgGly: 3.723 ± 0.514
1.359ArgHis: 1.359 ± 0.334
3.664ArgIle: 3.664 ± 0.455
3.428ArgLys: 3.428 ± 0.553
6.205ArgLeu: 6.205 ± 0.581
2.187ArgMet: 2.187 ± 0.381
2.364ArgAsn: 2.364 ± 0.449
2.955ArgPro: 2.955 ± 0.429
2.659ArgGln: 2.659 ± 0.47
4.196ArgArg: 4.196 ± 0.496
3.309ArgSer: 3.309 ± 0.458
4.078ArgThr: 4.078 ± 0.428
5.142ArgVal: 5.142 ± 0.579
1.064ArgTrp: 1.064 ± 0.219
1.655ArgTyr: 1.655 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
5.378SerAla: 5.378 ± 0.539
0.532SerCys: 0.532 ± 0.152
4.019SerAsp: 4.019 ± 0.413
2.482SerGlu: 2.482 ± 0.389
1.891SerPhe: 1.891 ± 0.345
5.082SerGly: 5.082 ± 0.514
0.532SerHis: 0.532 ± 0.148
3.25SerIle: 3.25 ± 0.437
2.482SerLys: 2.482 ± 0.452
4.196SerLeu: 4.196 ± 0.491
1.891SerMet: 1.891 ± 0.353
2.6SerAsn: 2.6 ± 0.407
2.896SerPro: 2.896 ± 0.412
2.778SerGln: 2.778 ± 0.378
3.369SerArg: 3.369 ± 0.594
3.369SerSer: 3.369 ± 0.463
3.487SerThr: 3.487 ± 0.479
3.96SerVal: 3.96 ± 0.522
0.886SerTrp: 0.886 ± 0.225
1.418SerTyr: 1.418 ± 0.337
0.0SerXaa: 0.0 ± 0.0
Thr
6.264ThrAla: 6.264 ± 0.626
0.65ThrCys: 0.65 ± 0.212
3.9ThrAsp: 3.9 ± 0.429
2.659ThrGlu: 2.659 ± 0.383
2.068ThrPhe: 2.068 ± 0.365
4.551ThrGly: 4.551 ± 0.573
0.886ThrHis: 0.886 ± 0.246
3.723ThrIle: 3.723 ± 0.456
2.896ThrLys: 2.896 ± 0.459
6.028ThrLeu: 6.028 ± 0.539
1.655ThrMet: 1.655 ± 0.299
2.187ThrAsn: 2.187 ± 0.408
3.841ThrPro: 3.841 ± 0.512
2.837ThrGln: 2.837 ± 0.364
3.841ThrArg: 3.841 ± 0.436
3.369ThrSer: 3.369 ± 0.45
4.669ThrThr: 4.669 ± 0.661
4.846ThrVal: 4.846 ± 0.644
1.3ThrTrp: 1.3 ± 0.258
2.246ThrTyr: 2.246 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
6.205ValAla: 6.205 ± 0.512
0.532ValCys: 0.532 ± 0.165
3.546ValAsp: 3.546 ± 0.37
5.082ValGlu: 5.082 ± 0.492
2.482ValPhe: 2.482 ± 0.382
4.669ValGly: 4.669 ± 0.48
0.768ValHis: 0.768 ± 0.195
4.137ValIle: 4.137 ± 0.501
3.25ValLys: 3.25 ± 0.457
4.787ValLeu: 4.787 ± 0.54
2.187ValMet: 2.187 ± 0.378
3.428ValAsn: 3.428 ± 0.374
2.896ValPro: 2.896 ± 0.504
2.896ValGln: 2.896 ± 0.428
5.555ValArg: 5.555 ± 0.553
4.491ValSer: 4.491 ± 0.539
5.142ValThr: 5.142 ± 0.58
4.905ValVal: 4.905 ± 0.678
0.946ValTrp: 0.946 ± 0.183
2.305ValTyr: 2.305 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
1.241TrpAla: 1.241 ± 0.253
0.355TrpCys: 0.355 ± 0.157
1.773TrpAsp: 1.773 ± 0.357
0.946TrpGlu: 0.946 ± 0.205
0.709TrpPhe: 0.709 ± 0.212
1.3TrpGly: 1.3 ± 0.289
0.295TrpHis: 0.295 ± 0.123
0.65TrpIle: 0.65 ± 0.229
0.591TrpLys: 0.591 ± 0.178
1.537TrpLeu: 1.537 ± 0.286
0.532TrpMet: 0.532 ± 0.152
0.946TrpAsn: 0.946 ± 0.219
0.591TrpPro: 0.591 ± 0.186
0.886TrpGln: 0.886 ± 0.226
0.886TrpArg: 0.886 ± 0.291
1.064TrpSer: 1.064 ± 0.272
0.946TrpThr: 0.946 ± 0.202
0.709TrpVal: 0.709 ± 0.244
0.414TrpTrp: 0.414 ± 0.156
0.591TrpTyr: 0.591 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.191TyrAla: 3.191 ± 0.376
0.414TyrCys: 0.414 ± 0.183
2.246TyrAsp: 2.246 ± 0.333
2.305TyrGlu: 2.305 ± 0.38
0.886TyrPhe: 0.886 ± 0.252
2.482TyrGly: 2.482 ± 0.381
0.532TyrHis: 0.532 ± 0.15
1.596TyrIle: 1.596 ± 0.343
1.3TyrLys: 1.3 ± 0.299
2.009TyrLeu: 2.009 ± 0.395
1.005TyrMet: 1.005 ± 0.253
1.537TyrAsn: 1.537 ± 0.316
2.128TyrPro: 2.128 ± 0.403
1.596TyrGln: 1.596 ± 0.328
2.6TyrArg: 2.6 ± 0.424
2.364TyrSer: 2.364 ± 0.433
2.6TyrThr: 2.6 ± 0.475
2.659TyrVal: 2.659 ± 0.433
0.532TyrTrp: 0.532 ± 0.203
1.005TyrTyr: 1.005 ± 0.271
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16922 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski