Amino acid dipepetide frequency for Thiovulum sp. ES

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.912AlaAla: 0.912 ± 0.057
0.326AlaCys: 0.326 ± 0.025
2.811AlaAsp: 2.811 ± 0.073
4.055AlaGlu: 4.055 ± 0.094
2.496AlaPhe: 2.496 ± 0.077
3.468AlaGly: 3.468 ± 0.09
0.76AlaHis: 0.76 ± 0.039
5.221AlaIle: 5.221 ± 0.111
4.835AlaLys: 4.835 ± 0.1
4.684AlaLeu: 4.684 ± 0.091
1.201AlaMet: 1.201 ± 0.046
2.763AlaAsn: 2.763 ± 0.072
1.274AlaPro: 1.274 ± 0.055
1.279AlaGln: 1.279 ± 0.046
1.685AlaArg: 1.685 ± 0.048
3.459AlaSer: 3.459 ± 0.105
3.256AlaThr: 3.256 ± 0.094
3.511AlaVal: 3.511 ± 0.085
0.39AlaTrp: 0.39 ± 0.061
1.663AlaTyr: 1.663 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.245CysAla: 0.245 ± 0.02
0.043CysCys: 0.043 ± 0.007
0.387CysAsp: 0.387 ± 0.027
0.531CysGlu: 0.531 ± 0.037
0.221CysPhe: 0.221 ± 0.019
0.511CysGly: 0.511 ± 0.03
0.142CysHis: 0.142 ± 0.019
0.277CysIle: 0.277 ± 0.026
0.402CysLys: 0.402 ± 0.026
0.365CysLeu: 0.365 ± 0.026
0.082CysMet: 0.082 ± 0.012
0.273CysAsn: 0.273 ± 0.02
0.261CysPro: 0.261 ± 0.026
0.226CysGln: 0.226 ± 0.022
0.267CysArg: 0.267 ± 0.026
0.462CysSer: 0.462 ± 0.028
0.207CysThr: 0.207 ± 0.018
0.315CysVal: 0.315 ± 0.026
0.033CysTrp: 0.033 ± 0.008
0.182CysTyr: 0.182 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
2.264AspAla: 2.264 ± 0.064
0.291AspCys: 0.291 ± 0.022
2.973AspAsp: 2.973 ± 0.087
5.738AspGlu: 5.738 ± 0.107
4.564AspPhe: 4.564 ± 0.121
4.102AspGly: 4.102 ± 0.123
0.664AspHis: 0.664 ± 0.033
5.39AspIle: 5.39 ± 0.105
4.325AspLys: 4.325 ± 0.088
6.043AspLeu: 6.043 ± 0.114
1.091AspMet: 1.091 ± 0.039
2.488AspAsn: 2.488 ± 0.077
1.309AspPro: 1.309 ± 0.045
1.039AspGln: 1.039 ± 0.041
2.172AspArg: 2.172 ± 0.065
4.412AspSer: 4.412 ± 0.092
2.888AspThr: 2.888 ± 0.074
3.286AspVal: 3.286 ± 0.084
0.618AspTrp: 0.618 ± 0.036
2.265AspTyr: 2.265 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
4.137GluAla: 4.137 ± 0.095
0.289GluCys: 0.289 ± 0.024
4.409GluAsp: 4.409 ± 0.102
6.43GluGlu: 6.43 ± 0.142
4.368GluPhe: 4.368 ± 0.089
3.067GluGly: 3.067 ± 0.08
1.167GluHis: 1.167 ± 0.048
9.334GluIle: 9.334 ± 0.173
9.799GluLys: 9.799 ± 0.179
7.497GluLeu: 7.497 ± 0.126
1.959GluMet: 1.959 ± 0.059
7.663GluAsn: 7.663 ± 0.162
1.655GluPro: 1.655 ± 0.06
2.0GluGln: 2.0 ± 0.056
3.242GluArg: 3.242 ± 0.073
4.688GluSer: 4.688 ± 0.103
5.085GluThr: 5.085 ± 0.113
5.457GluVal: 5.457 ± 0.106
0.618GluTrp: 0.618 ± 0.032
2.39GluTyr: 2.39 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
3.095PheAla: 3.095 ± 0.084
0.319PheCys: 0.319 ± 0.024
3.577PheAsp: 3.577 ± 0.084
4.584PheGlu: 4.584 ± 0.108
3.03PhePhe: 3.03 ± 0.09
4.209PheGly: 4.209 ± 0.104
1.045PheHis: 1.045 ± 0.044
3.908PheIle: 3.908 ± 0.096
4.254PheLys: 4.254 ± 0.09
5.412PheLeu: 5.412 ± 0.12
1.122PheMet: 1.122 ± 0.047
2.567PheAsn: 2.567 ± 0.068
1.658PhePro: 1.658 ± 0.057
2.191PheGln: 2.191 ± 0.072
2.011PheArg: 2.011 ± 0.064
5.3PheSer: 5.3 ± 0.108
2.539PheThr: 2.539 ± 0.074
3.465PheVal: 3.465 ± 0.079
0.56PheTrp: 0.56 ± 0.036
2.151PheTyr: 2.151 ± 0.059
0.002PheXaa: 0.002 ± 0.001
Gly
3.419GlyAla: 3.419 ± 0.088
0.39GlyCys: 0.39 ± 0.029
3.868GlyAsp: 3.868 ± 0.092
5.19GlyGlu: 5.19 ± 0.111
3.644GlyPhe: 3.644 ± 0.086
4.48GlyGly: 4.48 ± 0.113
0.993GlyHis: 0.993 ± 0.047
5.269GlyIle: 5.269 ± 0.083
5.484GlyLys: 5.484 ± 0.095
4.646GlyLeu: 4.646 ± 0.092
1.34GlyMet: 1.34 ± 0.046
3.639GlyAsn: 3.639 ± 0.117
0.582GlyPro: 0.582 ± 0.035
1.276GlyGln: 1.276 ± 0.058
2.194GlyArg: 2.194 ± 0.061
3.808GlySer: 3.808 ± 0.112
3.392GlyThr: 3.392 ± 0.095
4.413GlyVal: 4.413 ± 0.085
0.569GlyTrp: 0.569 ± 0.031
2.374GlyTyr: 2.374 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
0.604HisAla: 0.604 ± 0.034
0.149HisCys: 0.149 ± 0.017
0.722HisAsp: 0.722 ± 0.035
0.977HisGlu: 0.977 ± 0.041
1.029HisPhe: 1.029 ± 0.034
1.042HisGly: 1.042 ± 0.041
0.373HisHis: 0.373 ± 0.023
1.366HisIle: 1.366 ± 0.051
1.307HisLys: 1.307 ± 0.049
1.582HisLeu: 1.582 ± 0.055
0.3HisMet: 0.3 ± 0.021
0.822HisAsn: 0.822 ± 0.036
0.586HisPro: 0.586 ± 0.031
0.534HisGln: 0.534 ± 0.03
0.722HisArg: 0.722 ± 0.036
1.227HisSer: 1.227 ± 0.043
0.904HisThr: 0.904 ± 0.044
0.621HisVal: 0.621 ± 0.035
0.13HisTrp: 0.13 ± 0.014
0.571HisTyr: 0.571 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.313IleAla: 5.313 ± 0.1
0.487IleCys: 0.487 ± 0.032
5.746IleAsp: 5.746 ± 0.098
7.893IleGlu: 7.893 ± 0.137
5.109IlePhe: 5.109 ± 0.115
5.368IleGly: 5.368 ± 0.109
1.303IleHis: 1.303 ± 0.055
5.349IleIle: 5.349 ± 0.11
5.455IleLys: 5.455 ± 0.118
8.389IleLeu: 8.389 ± 0.143
1.321IleMet: 1.321 ± 0.045
3.637IleAsn: 3.637 ± 0.084
3.005IlePro: 3.005 ± 0.069
2.227IleGln: 2.227 ± 0.065
3.146IleArg: 3.146 ± 0.077
8.155IleSer: 8.155 ± 0.143
4.013IleThr: 4.013 ± 0.099
5.166IleVal: 5.166 ± 0.098
0.561IleTrp: 0.561 ± 0.03
3.399IleTyr: 3.399 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
4.072LysAla: 4.072 ± 0.097
0.365LysCys: 0.365 ± 0.029
5.141LysAsp: 5.141 ± 0.092
7.58LysGlu: 7.58 ± 0.141
4.322LysPhe: 4.322 ± 0.088
3.961LysGly: 3.961 ± 0.078
1.249LysHis: 1.249 ± 0.044
8.756LysIle: 8.756 ± 0.141
7.886LysLys: 7.886 ± 0.166
7.322LysLeu: 7.322 ± 0.131
2.113LysMet: 2.113 ± 0.061
6.334LysAsn: 6.334 ± 0.126
2.259LysPro: 2.259 ± 0.06
2.004LysGln: 2.004 ± 0.061
3.661LysArg: 3.661 ± 0.09
6.201LysSer: 6.201 ± 0.103
4.761LysThr: 4.761 ± 0.095
5.178LysVal: 5.178 ± 0.089
0.563LysTrp: 0.563 ± 0.036
3.283LysTyr: 3.283 ± 0.08
0.0LysXaa: 0.0 ± 0.0
Leu
4.774LeuAla: 4.774 ± 0.09
0.384LeuCys: 0.384 ± 0.024
5.604LeuAsp: 5.604 ± 0.094
8.585LeuGlu: 8.585 ± 0.155
5.272LeuPhe: 5.272 ± 0.122
5.191LeuGly: 5.191 ± 0.113
1.604LeuHis: 1.604 ± 0.058
6.538LeuIle: 6.538 ± 0.097
8.841LeuLys: 8.841 ± 0.141
7.984LeuLeu: 7.984 ± 0.133
1.543LeuMet: 1.543 ± 0.05
5.586LeuAsn: 5.586 ± 0.111
2.969LeuPro: 2.969 ± 0.069
3.454LeuGln: 3.454 ± 0.086
3.25LeuArg: 3.25 ± 0.076
8.074LeuSer: 8.074 ± 0.142
4.238LeuThr: 4.238 ± 0.104
4.728LeuVal: 4.728 ± 0.075
0.593LeuTrp: 0.593 ± 0.031
3.138LeuTyr: 3.138 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.246MetAla: 1.246 ± 0.056
0.074MetCys: 0.074 ± 0.012
0.929MetAsp: 0.929 ± 0.042
1.568MetGlu: 1.568 ± 0.049
0.975MetPhe: 0.975 ± 0.042
1.314MetGly: 1.314 ± 0.056
0.277MetHis: 0.277 ± 0.022
1.603MetIle: 1.603 ± 0.057
2.117MetLys: 2.117 ± 0.058
1.758MetLeu: 1.758 ± 0.064
0.481MetMet: 0.481 ± 0.03
1.261MetAsn: 1.261 ± 0.049
0.705MetPro: 0.705 ± 0.03
0.563MetGln: 0.563 ± 0.032
0.983MetArg: 0.983 ± 0.045
1.426MetSer: 1.426 ± 0.051
1.016MetThr: 1.016 ± 0.039
1.17MetVal: 1.17 ± 0.046
0.13MetTrp: 0.13 ± 0.015
0.432MetTyr: 0.432 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.444AsnAla: 2.444 ± 0.087
0.367AsnCys: 0.367 ± 0.025
2.615AsnAsp: 2.615 ± 0.086
3.917AsnGlu: 3.917 ± 0.089
3.897AsnPhe: 3.897 ± 0.086
4.244AsnGly: 4.244 ± 0.113
0.82AsnHis: 0.82 ± 0.037
5.34AsnIle: 5.34 ± 0.096
3.538AsnLys: 3.538 ± 0.079
6.897AsnLeu: 6.897 ± 0.131
1.31AsnMet: 1.31 ± 0.051
2.496AsnAsn: 2.496 ± 0.082
2.074AsnPro: 2.074 ± 0.056
1.606AsnGln: 1.606 ± 0.053
2.95AsnArg: 2.95 ± 0.081
5.675AsnSer: 5.675 ± 0.123
2.49AsnThr: 2.49 ± 0.081
2.608AsnVal: 2.608 ± 0.065
0.591AsnTrp: 0.591 ± 0.032
2.246AsnTyr: 2.246 ± 0.059
0.002AsnXaa: 0.002 ± 0.002
Pro
1.274ProAla: 1.274 ± 0.054
0.141ProCys: 0.141 ± 0.014
1.603ProAsp: 1.603 ± 0.058
2.741ProGlu: 2.741 ± 0.066
1.622ProPhe: 1.622 ± 0.054
0.677ProGly: 0.677 ± 0.041
0.656ProHis: 0.656 ± 0.033
2.485ProIle: 2.485 ± 0.066
2.768ProLys: 2.768 ± 0.075
2.328ProLeu: 2.328 ± 0.056
0.588ProMet: 0.588 ± 0.027
1.767ProAsn: 1.767 ± 0.057
0.803ProPro: 0.803 ± 0.044
0.915ProGln: 0.915 ± 0.047
0.839ProArg: 0.839 ± 0.038
1.576ProSer: 1.576 ± 0.054
1.894ProThr: 1.894 ± 0.064
1.804ProVal: 1.804 ± 0.058
0.194ProTrp: 0.194 ± 0.019
1.127ProTyr: 1.127 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
1.247GlnAla: 1.247 ± 0.05
0.117GlnCys: 0.117 ± 0.014
1.205GlnAsp: 1.205 ± 0.043
1.729GlnGlu: 1.729 ± 0.056
1.518GlnPhe: 1.518 ± 0.049
1.095GlnGly: 1.095 ± 0.045
0.433GlnHis: 0.433 ± 0.025
2.871GlnIle: 2.871 ± 0.069
3.859GlnLys: 3.859 ± 0.1
2.232GlnLeu: 2.232 ± 0.068
0.656GlnMet: 0.656 ± 0.032
2.562GlnAsn: 2.562 ± 0.078
0.675GlnPro: 0.675 ± 0.033
0.803GlnGln: 0.803 ± 0.043
1.035GlnArg: 1.035 ± 0.039
1.609GlnSer: 1.609 ± 0.057
1.75GlnThr: 1.75 ± 0.056
1.29GlnVal: 1.29 ± 0.046
0.166GlnTrp: 0.166 ± 0.015
0.871GlnTyr: 0.871 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
1.842ArgAla: 1.842 ± 0.058
0.198ArgCys: 0.198 ± 0.019
2.866ArgAsp: 2.866 ± 0.067
4.096ArgGlu: 4.096 ± 0.098
2.064ArgPhe: 2.064 ± 0.06
2.376ArgGly: 2.376 ± 0.077
0.563ArgHis: 0.563 ± 0.032
3.139ArgIle: 3.139 ± 0.071
3.389ArgLys: 3.389 ± 0.087
2.961ArgLeu: 2.961 ± 0.074
0.762ArgMet: 0.762 ± 0.04
2.419ArgAsn: 2.419 ± 0.061
0.931ArgPro: 0.931 ± 0.047
0.993ArgGln: 0.993 ± 0.043
1.385ArgArg: 1.385 ± 0.062
1.805ArgSer: 1.805 ± 0.054
1.65ArgThr: 1.65 ± 0.054
3.0ArgVal: 3.0 ± 0.075
0.304ArgTrp: 0.304 ± 0.024
1.408ArgTyr: 1.408 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.219SerAla: 4.219 ± 0.089
0.484SerCys: 0.484 ± 0.031
4.538SerAsp: 4.538 ± 0.122
6.938SerGlu: 6.938 ± 0.122
4.375SerPhe: 4.375 ± 0.094
5.687SerGly: 5.687 ± 0.129
1.168SerHis: 1.168 ± 0.044
5.958SerIle: 5.958 ± 0.118
6.165SerLys: 6.165 ± 0.116
7.148SerLeu: 7.148 ± 0.136
1.35SerMet: 1.35 ± 0.049
3.936SerAsn: 3.936 ± 0.099
2.101SerPro: 2.101 ± 0.059
2.297SerGln: 2.297 ± 0.06
2.771SerArg: 2.771 ± 0.067
5.83SerSer: 5.83 ± 0.139
3.708SerThr: 3.708 ± 0.145
4.518SerVal: 4.518 ± 0.1
0.58SerTrp: 0.58 ± 0.039
2.572SerTyr: 2.572 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
3.139ThrAla: 3.139 ± 0.161
0.337ThrCys: 0.337 ± 0.025
2.946ThrAsp: 2.946 ± 0.084
4.132ThrGlu: 4.132 ± 0.1
2.749ThrPhe: 2.749 ± 0.076
3.345ThrGly: 3.345 ± 0.086
0.782ThrHis: 0.782 ± 0.035
4.424ThrIle: 4.424 ± 0.104
3.953ThrLys: 3.953 ± 0.091
5.205ThrLeu: 5.205 ± 0.105
0.873ThrMet: 0.873 ± 0.043
2.643ThrAsn: 2.643 ± 0.091
1.924ThrPro: 1.924 ± 0.058
1.378ThrGln: 1.378 ± 0.051
1.574ThrArg: 1.574 ± 0.055
3.566ThrSer: 3.566 ± 0.09
2.994ThrThr: 2.994 ± 0.106
3.623ThrVal: 3.623 ± 0.108
0.348ThrTrp: 0.348 ± 0.024
1.726ThrTyr: 1.726 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
3.936ValAla: 3.936 ± 0.091
0.4ValCys: 0.4 ± 0.028
3.697ValAsp: 3.697 ± 0.084
5.683ValGlu: 5.683 ± 0.108
3.044ValPhe: 3.044 ± 0.079
4.059ValGly: 4.059 ± 0.086
0.787ValHis: 0.787 ± 0.042
4.33ValIle: 4.33 ± 0.083
4.671ValLys: 4.671 ± 0.095
5.553ValLeu: 5.553 ± 0.105
1.118ValMet: 1.118 ± 0.043
2.763ValAsn: 2.763 ± 0.077
1.846ValPro: 1.846 ± 0.056
1.581ValGln: 1.581 ± 0.054
2.202ValArg: 2.202 ± 0.052
5.523ValSer: 5.523 ± 0.119
2.853ValThr: 2.853 ± 0.079
4.349ValVal: 4.349 ± 0.101
0.569ValTrp: 0.569 ± 0.032
1.623ValTyr: 1.623 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.409TrpAla: 0.409 ± 0.027
0.07TrpCys: 0.07 ± 0.01
0.364TrpAsp: 0.364 ± 0.03
0.571TrpGlu: 0.571 ± 0.033
0.402TrpPhe: 0.402 ± 0.027
0.602TrpGly: 0.602 ± 0.078
0.158TrpHis: 0.158 ± 0.019
0.64TrpIle: 0.64 ± 0.035
0.888TrpLys: 0.888 ± 0.045
0.748TrpLeu: 0.748 ± 0.035
0.133TrpMet: 0.133 ± 0.014
0.525TrpAsn: 0.525 ± 0.032
0.063TrpPro: 0.063 ± 0.011
0.261TrpGln: 0.261 ± 0.019
0.43TrpArg: 0.43 ± 0.027
0.525TrpSer: 0.525 ± 0.03
0.335TrpThr: 0.335 ± 0.026
0.484TrpVal: 0.484 ± 0.027
0.082TrpTrp: 0.082 ± 0.01
0.254TrpTyr: 0.254 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.459TyrAla: 1.459 ± 0.057
0.202TyrCys: 0.202 ± 0.017
1.921TyrAsp: 1.921 ± 0.063
2.316TyrGlu: 2.316 ± 0.061
2.3TyrPhe: 2.3 ± 0.063
2.17TyrGly: 2.17 ± 0.06
0.624TyrHis: 0.624 ± 0.033
2.845TyrIle: 2.845 ± 0.079
3.075TyrLys: 3.075 ± 0.075
3.595TyrLeu: 3.595 ± 0.079
0.683TyrMet: 0.683 ± 0.036
1.977TyrAsn: 1.977 ± 0.066
1.122TyrPro: 1.122 ± 0.042
1.097TyrGln: 1.097 ± 0.039
1.63TyrArg: 1.63 ± 0.065
3.022TyrSer: 3.022 ± 0.082
1.688TyrThr: 1.688 ± 0.064
1.603TyrVal: 1.603 ± 0.059
0.345TyrTrp: 0.345 ± 0.025
1.359TyrTyr: 1.359 ± 0.062
0.002TyrXaa: 0.002 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.002XaaGlu: 0.002 ± 0.001
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.003XaaLys: 0.003 ± 0.002
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2104 proteins (632618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski