Amino acid dipepetide frequency for Micromonospora sp. HK10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.478AlaAla: 24.478 ± 0.195
0.929AlaCys: 0.929 ± 0.022
9.148AlaAsp: 9.148 ± 0.079
8.829AlaGlu: 8.829 ± 0.084
3.354AlaPhe: 3.354 ± 0.047
15.765AlaGly: 15.765 ± 0.13
2.596AlaHis: 2.596 ± 0.041
3.823AlaIle: 3.823 ± 0.053
2.311AlaLys: 2.311 ± 0.054
15.18AlaLeu: 15.18 ± 0.136
2.334AlaMet: 2.334 ± 0.039
2.134AlaAsn: 2.134 ± 0.044
7.441AlaPro: 7.441 ± 0.089
3.615AlaGln: 3.615 ± 0.046
10.961AlaArg: 10.961 ± 0.11
5.16AlaSer: 5.16 ± 0.061
8.091AlaThr: 8.091 ± 0.082
13.185AlaVal: 13.185 ± 0.115
1.96AlaTrp: 1.96 ± 0.04
2.92AlaTyr: 2.92 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.968CysAla: 0.968 ± 0.023
0.112CysCys: 0.112 ± 0.008
0.471CysAsp: 0.471 ± 0.015
0.292CysGlu: 0.292 ± 0.013
0.208CysPhe: 0.208 ± 0.012
0.84CysGly: 0.84 ± 0.022
0.182CysHis: 0.182 ± 0.01
0.129CysIle: 0.129 ± 0.008
0.081CysLys: 0.081 ± 0.007
0.682CysLeu: 0.682 ± 0.02
0.092CysMet: 0.092 ± 0.007
0.135CysAsn: 0.135 ± 0.01
0.486CysPro: 0.486 ± 0.019
0.194CysGln: 0.194 ± 0.011
0.634CysArg: 0.634 ± 0.02
0.37CysSer: 0.37 ± 0.014
0.418CysThr: 0.418 ± 0.017
0.621CysVal: 0.621 ± 0.02
0.135CysTrp: 0.135 ± 0.009
0.146CysTyr: 0.146 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.796AspAla: 7.796 ± 0.073
0.391AspCys: 0.391 ± 0.014
3.569AspAsp: 3.569 ± 0.053
3.76AspGlu: 3.76 ± 0.054
1.462AspPhe: 1.462 ± 0.028
6.549AspGly: 6.549 ± 0.07
1.272AspHis: 1.272 ± 0.031
1.537AspIle: 1.537 ± 0.033
0.89AspLys: 0.89 ± 0.029
6.934AspLeu: 6.934 ± 0.074
0.639AspMet: 0.639 ± 0.019
0.916AspAsn: 0.916 ± 0.023
5.172AspPro: 5.172 ± 0.06
1.756AspGln: 1.756 ± 0.035
5.863AspArg: 5.863 ± 0.059
2.085AspSer: 2.085 ± 0.039
2.781AspThr: 2.781 ± 0.044
4.928AspVal: 4.928 ± 0.058
1.064AspTrp: 1.064 ± 0.025
1.102AspTyr: 1.102 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
6.508GluAla: 6.508 ± 0.069
0.32GluCys: 0.32 ± 0.013
2.045GluAsp: 2.045 ± 0.032
2.607GluGlu: 2.607 ± 0.049
1.376GluPhe: 1.376 ± 0.028
3.095GluGly: 3.095 ± 0.043
1.375GluHis: 1.375 ± 0.027
2.141GluIle: 2.141 ± 0.039
0.952GluLys: 0.952 ± 0.027
6.771GluLeu: 6.771 ± 0.069
0.777GluMet: 0.777 ± 0.018
0.793GluAsn: 0.793 ± 0.026
3.498GluPro: 3.498 ± 0.051
2.223GluGln: 2.223 ± 0.045
5.166GluArg: 5.166 ± 0.06
2.042GluSer: 2.042 ± 0.037
2.451GluThr: 2.451 ± 0.039
4.787GluVal: 4.787 ± 0.05
0.695GluTrp: 0.695 ± 0.021
1.013GluTyr: 1.013 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.79PheAla: 3.79 ± 0.05
0.226PheCys: 0.226 ± 0.012
1.948PheAsp: 1.948 ± 0.033
1.138PheGlu: 1.138 ± 0.026
0.842PhePhe: 0.842 ± 0.023
2.942PheGly: 2.942 ± 0.038
0.557PheHis: 0.557 ± 0.018
0.681PheIle: 0.681 ± 0.021
0.385PheLys: 0.385 ± 0.018
2.512PheLeu: 2.512 ± 0.043
0.292PheMet: 0.292 ± 0.012
0.544PheAsn: 0.544 ± 0.02
1.353PhePro: 1.353 ± 0.026
0.674PheGln: 0.674 ± 0.022
1.829PheArg: 1.829 ± 0.033
1.261PheSer: 1.261 ± 0.029
1.898PheThr: 1.898 ± 0.034
2.404PheVal: 2.404 ± 0.041
0.439PheTrp: 0.439 ± 0.017
0.589PheTyr: 0.589 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.302GlyAla: 11.302 ± 0.098
0.824GlyCys: 0.824 ± 0.021
5.331GlyAsp: 5.331 ± 0.059
4.689GlyGlu: 4.689 ± 0.055
2.841GlyPhe: 2.841 ± 0.042
9.149GlyGly: 9.149 ± 0.108
2.176GlyHis: 2.176 ± 0.037
3.135GlyIle: 3.135 ± 0.046
1.862GlyLys: 1.862 ± 0.041
9.878GlyLeu: 9.878 ± 0.097
1.866GlyMet: 1.866 ± 0.036
1.733GlyAsn: 1.733 ± 0.035
5.637GlyPro: 5.637 ± 0.075
2.822GlyGln: 2.822 ± 0.045
8.449GlyArg: 8.449 ± 0.088
4.644GlySer: 4.644 ± 0.064
5.952GlyThr: 5.952 ± 0.061
8.125GlyVal: 8.125 ± 0.074
2.065GlyTrp: 2.065 ± 0.036
2.522GlyTyr: 2.522 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.578HisAla: 2.578 ± 0.049
0.178HisCys: 0.178 ± 0.008
1.306HisAsp: 1.306 ± 0.029
0.996HisGlu: 0.996 ± 0.022
0.538HisPhe: 0.538 ± 0.021
2.134HisGly: 2.134 ± 0.035
0.625HisHis: 0.625 ± 0.018
0.49HisIle: 0.49 ± 0.018
0.241HisLys: 0.241 ± 0.012
2.566HisLeu: 2.566 ± 0.037
0.25HisMet: 0.25 ± 0.012
0.347HisAsn: 0.347 ± 0.015
1.867HisPro: 1.867 ± 0.045
0.611HisGln: 0.611 ± 0.019
2.178HisArg: 2.178 ± 0.042
0.811HisSer: 0.811 ± 0.024
1.124HisThr: 1.124 ± 0.032
1.644HisVal: 1.644 ± 0.032
0.365HisTrp: 0.365 ± 0.013
0.42HisTyr: 0.42 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.639IleAla: 4.639 ± 0.058
0.27IleCys: 0.27 ± 0.012
2.217IleAsp: 2.217 ± 0.034
1.88IleGlu: 1.88 ± 0.04
0.861IlePhe: 0.861 ± 0.024
3.435IleGly: 3.435 ± 0.047
0.567IleHis: 0.567 ± 0.018
0.933IleIle: 0.933 ± 0.029
0.63IleLys: 0.63 ± 0.025
2.345IleLeu: 2.345 ± 0.035
0.433IleMet: 0.433 ± 0.016
0.715IleAsn: 0.715 ± 0.024
1.567IlePro: 1.567 ± 0.033
0.674IleGln: 0.674 ± 0.022
2.405IleArg: 2.405 ± 0.043
1.616IleSer: 1.616 ± 0.025
2.02IleThr: 2.02 ± 0.038
2.683IleVal: 2.683 ± 0.045
0.411IleTrp: 0.411 ± 0.015
0.555IleTyr: 0.555 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
2.071LysAla: 2.071 ± 0.054
0.096LysCys: 0.096 ± 0.008
0.834LysAsp: 0.834 ± 0.027
0.776LysGlu: 0.776 ± 0.026
0.391LysPhe: 0.391 ± 0.016
1.243LysGly: 1.243 ± 0.036
0.305LysHis: 0.305 ± 0.014
0.736LysIle: 0.736 ± 0.028
0.505LysLys: 0.505 ± 0.026
1.797LysLeu: 1.797 ± 0.04
0.287LysMet: 0.287 ± 0.015
0.338LysAsn: 0.338 ± 0.017
1.074LysPro: 1.074 ± 0.031
0.604LysGln: 0.604 ± 0.021
1.184LysArg: 1.184 ± 0.028
0.833LysSer: 0.833 ± 0.024
0.936LysThr: 0.936 ± 0.031
1.56LysVal: 1.56 ± 0.037
0.207LysTrp: 0.207 ± 0.011
0.316LysTyr: 0.316 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
17.745LeuAla: 17.745 ± 0.149
0.727LeuCys: 0.727 ± 0.02
7.217LeuAsp: 7.217 ± 0.09
3.836LeuGlu: 3.836 ± 0.053
2.621LeuPhe: 2.621 ± 0.045
9.607LeuGly: 9.607 ± 0.102
2.415LeuHis: 2.415 ± 0.045
3.309LeuIle: 3.309 ± 0.048
1.378LeuLys: 1.378 ± 0.033
12.313LeuLeu: 12.313 ± 0.129
1.336LeuMet: 1.336 ± 0.029
1.642LeuAsn: 1.642 ± 0.03
6.966LeuPro: 6.966 ± 0.066
1.878LeuGln: 1.878 ± 0.034
9.692LeuArg: 9.692 ± 0.087
4.686LeuSer: 4.686 ± 0.057
7.022LeuThr: 7.022 ± 0.073
10.306LeuVal: 10.306 ± 0.102
1.26LeuTrp: 1.26 ± 0.028
1.746LeuTyr: 1.746 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
2.093MetAla: 2.093 ± 0.033
0.105MetCys: 0.105 ± 0.007
0.702MetAsp: 0.702 ± 0.019
0.574MetGlu: 0.574 ± 0.022
0.418MetPhe: 0.418 ± 0.016
1.056MetGly: 1.056 ± 0.028
0.283MetHis: 0.283 ± 0.012
0.634MetIle: 0.634 ± 0.019
0.325MetLys: 0.325 ± 0.014
1.603MetLeu: 1.603 ± 0.033
0.234MetMet: 0.234 ± 0.013
0.334MetAsn: 0.334 ± 0.013
1.019MetPro: 1.019 ± 0.025
0.381MetGln: 0.381 ± 0.015
1.315MetArg: 1.315 ± 0.025
1.144MetSer: 1.144 ± 0.025
1.526MetThr: 1.526 ± 0.031
1.225MetVal: 1.225 ± 0.028
0.191MetTrp: 0.191 ± 0.012
0.279MetTyr: 0.279 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.115AsnAla: 2.115 ± 0.037
0.138AsnCys: 0.138 ± 0.009
0.899AsnAsp: 0.899 ± 0.023
0.736AsnGlu: 0.736 ± 0.021
0.498AsnPhe: 0.498 ± 0.017
1.851AsnGly: 1.851 ± 0.044
0.363AsnHis: 0.363 ± 0.014
0.571AsnIle: 0.571 ± 0.017
0.318AsnLys: 0.318 ± 0.015
1.855AsnLeu: 1.855 ± 0.032
0.265AsnMet: 0.265 ± 0.013
0.429AsnAsn: 0.429 ± 0.019
1.381AsnPro: 1.381 ± 0.033
0.553AsnGln: 0.553 ± 0.019
1.366AsnArg: 1.366 ± 0.029
0.8AsnSer: 0.8 ± 0.026
0.994AsnThr: 0.994 ± 0.031
1.361AsnVal: 1.361 ± 0.031
0.325AsnTrp: 0.325 ± 0.015
0.42AsnTyr: 0.42 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
10.969ProAla: 10.969 ± 0.12
0.291ProCys: 0.291 ± 0.013
4.88ProAsp: 4.88 ± 0.057
3.907ProGlu: 3.907 ± 0.051
1.425ProPhe: 1.425 ± 0.027
6.895ProGly: 6.895 ± 0.07
1.225ProHis: 1.225 ± 0.029
1.545ProIle: 1.545 ± 0.033
0.952ProLys: 0.952 ± 0.027
5.386ProLeu: 5.386 ± 0.052
0.959ProMet: 0.959 ± 0.025
0.964ProAsn: 0.964 ± 0.029
4.307ProPro: 4.307 ± 0.077
1.546ProGln: 1.546 ± 0.032
4.092ProArg: 4.092 ± 0.051
2.715ProSer: 2.715 ± 0.045
3.826ProThr: 3.826 ± 0.054
6.12ProVal: 6.12 ± 0.066
0.971ProTrp: 0.971 ± 0.025
1.32ProTyr: 1.32 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.667GlnAla: 3.667 ± 0.047
0.145GlnCys: 0.145 ± 0.01
1.116GlnAsp: 1.116 ± 0.026
1.169GlnGlu: 1.169 ± 0.024
0.711GlnPhe: 0.711 ± 0.021
1.91GlnGly: 1.91 ± 0.041
0.612GlnHis: 0.612 ± 0.02
1.014GlnIle: 1.014 ± 0.027
0.442GlnLys: 0.442 ± 0.018
3.4GlnLeu: 3.4 ± 0.051
0.41GlnMet: 0.41 ± 0.015
0.447GlnAsn: 0.447 ± 0.016
1.904GlnPro: 1.904 ± 0.04
1.168GlnGln: 1.168 ± 0.033
2.733GlnArg: 2.733 ± 0.037
1.053GlnSer: 1.053 ± 0.026
1.266GlnThr: 1.266 ± 0.03
2.795GlnVal: 2.795 ± 0.041
0.477GlnTrp: 0.477 ± 0.018
0.525GlnTyr: 0.525 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.992ArgAla: 10.992 ± 0.111
0.648ArgCys: 0.648 ± 0.022
4.832ArgAsp: 4.832 ± 0.055
4.404ArgGlu: 4.404 ± 0.053
2.447ArgPhe: 2.447 ± 0.038
5.905ArgGly: 5.905 ± 0.069
2.227ArgHis: 2.227 ± 0.038
3.113ArgIle: 3.113 ± 0.048
1.26ArgLys: 1.26 ± 0.028
9.865ArgLeu: 9.865 ± 0.093
1.86ArgMet: 1.86 ± 0.036
1.424ArgAsn: 1.424 ± 0.033
5.758ArgPro: 5.758 ± 0.065
2.675ArgGln: 2.675 ± 0.036
9.106ArgArg: 9.106 ± 0.109
3.948ArgSer: 3.948 ± 0.055
4.853ArgThr: 4.853 ± 0.054
6.544ArgVal: 6.544 ± 0.061
1.786ArgTrp: 1.786 ± 0.032
2.186ArgTyr: 2.186 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
5.999SerAla: 5.999 ± 0.06
0.375SerCys: 0.375 ± 0.017
2.191SerAsp: 2.191 ± 0.04
1.751SerGlu: 1.751 ± 0.034
1.325SerPhe: 1.325 ± 0.029
5.114SerGly: 5.114 ± 0.067
0.8SerHis: 0.8 ± 0.021
1.451SerIle: 1.451 ± 0.032
0.703SerLys: 0.703 ± 0.022
4.108SerLeu: 4.108 ± 0.052
0.945SerMet: 0.945 ± 0.022
0.833SerAsn: 0.833 ± 0.026
2.965SerPro: 2.965 ± 0.049
1.06SerGln: 1.06 ± 0.028
3.457SerArg: 3.457 ± 0.041
2.285SerSer: 2.285 ± 0.044
2.893SerThr: 2.893 ± 0.047
3.764SerVal: 3.764 ± 0.047
0.886SerTrp: 0.886 ± 0.025
1.162SerTyr: 1.162 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
8.708ThrAla: 8.708 ± 0.091
0.408ThrCys: 0.408 ± 0.018
3.607ThrAsp: 3.607 ± 0.052
2.884ThrGlu: 2.884 ± 0.039
1.607ThrPhe: 1.607 ± 0.032
6.764ThrGly: 6.764 ± 0.069
1.036ThrHis: 1.036 ± 0.027
1.887ThrIle: 1.887 ± 0.041
0.893ThrLys: 0.893 ± 0.031
5.8ThrLeu: 5.8 ± 0.063
0.92ThrMet: 0.92 ± 0.022
1.066ThrAsn: 1.066 ± 0.031
4.104ThrPro: 4.104 ± 0.048
1.174ThrGln: 1.174 ± 0.026
4.141ThrArg: 4.141 ± 0.044
2.731ThrSer: 2.731 ± 0.053
3.551ThrThr: 3.551 ± 0.053
6.316ThrVal: 6.316 ± 0.067
0.936ThrTrp: 0.936 ± 0.024
1.201ThrTyr: 1.201 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
13.021ValAla: 13.021 ± 0.126
0.688ValCys: 0.688 ± 0.019
5.964ValAsp: 5.964 ± 0.065
4.767ValGlu: 4.767 ± 0.057
2.276ValPhe: 2.276 ± 0.043
7.581ValGly: 7.581 ± 0.077
1.811ValHis: 1.811 ± 0.033
2.818ValIle: 2.818 ± 0.048
1.425ValLys: 1.425 ± 0.038
10.213ValLeu: 10.213 ± 0.104
1.116ValMet: 1.116 ± 0.028
1.686ValAsn: 1.686 ± 0.033
5.574ValPro: 5.574 ± 0.065
1.985ValGln: 1.985 ± 0.038
7.572ValArg: 7.572 ± 0.075
4.034ValSer: 4.034 ± 0.053
5.989ValThr: 5.989 ± 0.06
8.788ValVal: 8.788 ± 0.092
1.143ValTrp: 1.143 ± 0.031
1.448ValTyr: 1.448 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.774TrpAla: 1.774 ± 0.036
0.163TrpCys: 0.163 ± 0.01
0.78TrpAsp: 0.78 ± 0.023
0.619TrpGlu: 0.619 ± 0.019
0.536TrpPhe: 0.536 ± 0.016
0.981TrpGly: 0.981 ± 0.025
0.397TrpHis: 0.397 ± 0.015
0.522TrpIle: 0.522 ± 0.019
0.252TrpLys: 0.252 ± 0.011
2.083TrpLeu: 2.083 ± 0.039
0.262TrpMet: 0.262 ± 0.012
0.384TrpAsn: 0.384 ± 0.017
0.963TrpPro: 0.963 ± 0.024
0.678TrpGln: 0.678 ± 0.02
1.695TrpArg: 1.695 ± 0.033
1.024TrpSer: 1.024 ± 0.028
1.074TrpThr: 1.074 ± 0.026
1.086TrpVal: 1.086 ± 0.025
0.394TrpTrp: 0.394 ± 0.017
0.407TrpTyr: 0.407 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.0TyrAla: 3.0 ± 0.042
0.163TyrCys: 0.163 ± 0.01
1.409TyrAsp: 1.409 ± 0.03
1.022TyrGlu: 1.022 ± 0.024
0.568TyrPhe: 0.568 ± 0.018
2.186TyrGly: 2.186 ± 0.04
0.444TyrHis: 0.444 ± 0.017
0.388TyrIle: 0.388 ± 0.017
0.284TyrLys: 0.284 ± 0.014
2.413TyrLeu: 2.413 ± 0.036
0.163TyrMet: 0.163 ± 0.01
0.374TyrAsn: 0.374 ± 0.02
1.258TyrPro: 1.258 ± 0.031
0.691TyrGln: 0.691 ± 0.02
1.996TyrArg: 1.996 ± 0.043
0.826TyrSer: 0.826 ± 0.023
1.071TyrThr: 1.071 ± 0.028
1.657TyrVal: 1.657 ± 0.034
0.362TyrTrp: 0.362 ± 0.014
0.439TyrTyr: 0.439 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5548 proteins (1776529 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski