Phialocephala subalpina

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Mollisiaceae; Phialocephala; Phialocephala fortinii species complex

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7X5P3|A0A1L7X5P3_9HELO Related to RSM24-mitochondrial ribosomal protein small subunit OS=Phialocephala subalpina OX=576137 GN=PAC_10252 PE=4 SV=1
MM1 pKa = 7.68LNPSRR6 pKa = 11.84DD7 pKa = 3.69PMPAEE12 pKa = 3.71QQFPTFVGVVEE23 pKa = 4.29STEE26 pKa = 4.29VYY28 pKa = 10.74DD29 pKa = 4.88FYY31 pKa = 11.72GNSALADD38 pKa = 3.76SLSQDD43 pKa = 3.91DD44 pKa = 5.12VLDD47 pKa = 4.34LLPFAGPIGGQTLDD61 pKa = 3.47STLGGGTSEE70 pKa = 4.5TFPSLLVDD78 pKa = 3.63AHH80 pKa = 6.35TSLDD84 pKa = 3.61NGFSLDD90 pKa = 3.44DD91 pKa = 3.81HH92 pKa = 6.83EE93 pKa = 5.75FFSFDD98 pKa = 3.98DD99 pKa = 3.44YY100 pKa = 11.22TYY102 pKa = 11.17PDD104 pKa = 4.25FEE106 pKa = 5.33LALQAYY112 pKa = 9.25GEE114 pKa = 4.34IAVTSHH120 pKa = 6.57SLQPSITHH128 pKa = 5.57QPSLPSDD135 pKa = 3.76LQSSGSSSTSSFPQIQDD152 pKa = 3.09SSGSAVPTSSLVSSKK167 pKa = 10.87SPSPASRR174 pKa = 11.84SSPASATKK182 pKa = 10.59LIISPSSFFRR192 pKa = 11.84CPVCC196 pKa = 5.18

Molecular weight: 20.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7X2E8|A0A1L7X2E8_9HELO Zn(2)-C6 fungal-type domain-containing protein OS=Phialocephala subalpina OX=576137 GN=PAC_09086 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.44QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20170

0

20170

9780577

12

7767

484.9

53.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.014

1.346 ± 0.008

5.481 ± 0.012

6.274 ± 0.018

3.903 ± 0.011

6.859 ± 0.017

2.299 ± 0.007

5.335 ± 0.011

5.075 ± 0.014

8.989 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.006

3.886 ± 0.01

5.738 ± 0.018

3.86 ± 0.012

5.658 ± 0.017

8.408 ± 0.028

6.158 ± 0.015

5.987 ± 0.011

1.58 ± 0.008

2.857 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski