Amino acid dipepetide frequency for Clostridium phage CPS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.537AlaCys: 0.537 ± 0.347
1.611AlaAsp: 1.611 ± 0.406
2.148AlaGlu: 2.148 ± 0.505
2.506AlaPhe: 2.506 ± 0.717
2.685AlaGly: 2.685 ± 0.88
1.432AlaHis: 1.432 ± 0.447
2.864AlaIle: 2.864 ± 0.924
2.327AlaLys: 2.327 ± 0.566
2.864AlaLeu: 2.864 ± 0.842
1.432AlaMet: 1.432 ± 0.702
1.432AlaAsn: 1.432 ± 0.379
1.79AlaPro: 1.79 ± 0.607
1.79AlaGln: 1.79 ± 0.675
1.969AlaArg: 1.969 ± 0.416
2.327AlaSer: 2.327 ± 0.649
3.222AlaThr: 3.222 ± 0.779
1.969AlaVal: 1.969 ± 0.889
0.895AlaTrp: 0.895 ± 0.398
2.148AlaTyr: 2.148 ± 0.498
0.0AlaXaa: 0.0 ± 0.0
Cys
0.537CysAla: 0.537 ± 0.279
0.358CysCys: 0.358 ± 0.214
1.074CysAsp: 1.074 ± 0.401
0.895CysGlu: 0.895 ± 0.348
0.716CysPhe: 0.716 ± 0.371
2.148CysGly: 2.148 ± 0.747
0.537CysHis: 0.537 ± 0.32
1.253CysIle: 1.253 ± 0.504
1.969CysLys: 1.969 ± 0.788
2.148CysLeu: 2.148 ± 0.525
0.179CysMet: 0.179 ± 0.195
0.895CysAsn: 0.895 ± 0.387
0.716CysPro: 0.716 ± 0.35
0.358CysGln: 0.358 ± 0.325
0.179CysArg: 0.179 ± 0.195
0.895CysSer: 0.895 ± 0.518
0.358CysThr: 0.358 ± 0.332
0.716CysVal: 0.716 ± 0.35
0.0CysTrp: 0.0 ± 0.0
0.895CysTyr: 0.895 ± 0.374
0.0CysXaa: 0.0 ± 0.0
Asp
2.327AspAla: 2.327 ± 0.524
1.253AspCys: 1.253 ± 0.548
2.864AspAsp: 2.864 ± 0.874
5.013AspGlu: 5.013 ± 0.639
3.759AspPhe: 3.759 ± 0.718
4.117AspGly: 4.117 ± 1.079
1.074AspHis: 1.074 ± 0.302
6.087AspIle: 6.087 ± 0.843
6.982AspLys: 6.982 ± 1.074
3.938AspLeu: 3.938 ± 0.926
1.969AspMet: 1.969 ± 0.542
3.759AspAsn: 3.759 ± 0.924
1.253AspPro: 1.253 ± 0.328
0.716AspGln: 0.716 ± 0.341
3.043AspArg: 3.043 ± 1.021
2.148AspSer: 2.148 ± 0.595
3.58AspThr: 3.58 ± 0.929
5.192AspVal: 5.192 ± 1.122
0.716AspTrp: 0.716 ± 0.335
1.969AspTyr: 1.969 ± 0.512
0.0AspXaa: 0.0 ± 0.0
Glu
0.358GluAla: 0.358 ± 0.245
1.253GluCys: 1.253 ± 0.521
2.685GluAsp: 2.685 ± 0.658
6.266GluGlu: 6.266 ± 1.387
2.685GluPhe: 2.685 ± 0.754
6.803GluGly: 6.803 ± 1.383
1.611GluHis: 1.611 ± 0.52
6.624GluIle: 6.624 ± 1.275
7.877GluLys: 7.877 ± 1.291
6.445GluLeu: 6.445 ± 1.261
2.148GluMet: 2.148 ± 0.703
6.445GluAsn: 6.445 ± 1.183
1.969GluPro: 1.969 ± 0.487
1.79GluGln: 1.79 ± 0.444
2.506GluArg: 2.506 ± 0.894
3.401GluSer: 3.401 ± 0.804
3.58GluThr: 3.58 ± 0.962
5.371GluVal: 5.371 ± 0.908
0.895GluTrp: 0.895 ± 0.479
4.117GluTyr: 4.117 ± 1.185
0.0GluXaa: 0.0 ± 0.0
Phe
1.611PheAla: 1.611 ± 0.531
0.358PheCys: 0.358 ± 0.228
3.759PheAsp: 3.759 ± 0.815
4.117PheGlu: 4.117 ± 0.902
2.327PhePhe: 2.327 ± 0.697
2.685PheGly: 2.685 ± 0.552
0.537PheHis: 0.537 ± 0.498
4.117PheIle: 4.117 ± 1.032
3.759PheLys: 3.759 ± 0.963
3.401PheLeu: 3.401 ± 0.608
2.327PheMet: 2.327 ± 0.686
4.117PheAsn: 4.117 ± 0.612
1.432PhePro: 1.432 ± 0.412
0.895PheGln: 0.895 ± 0.352
1.253PheArg: 1.253 ± 0.57
1.969PheSer: 1.969 ± 0.402
1.79PheThr: 1.79 ± 0.77
2.506PheVal: 2.506 ± 0.651
0.537PheTrp: 0.537 ± 0.365
1.79PheTyr: 1.79 ± 0.682
0.0PheXaa: 0.0 ± 0.0
Gly
4.296GlyAla: 4.296 ± 0.763
1.432GlyCys: 1.432 ± 0.54
5.729GlyAsp: 5.729 ± 1.225
3.938GlyGlu: 3.938 ± 0.698
4.475GlyPhe: 4.475 ± 0.84
5.013GlyGly: 5.013 ± 0.995
0.716GlyHis: 0.716 ± 0.442
5.908GlyIle: 5.908 ± 1.307
6.982GlyLys: 6.982 ± 1.163
5.908GlyLeu: 5.908 ± 1.013
2.506GlyMet: 2.506 ± 0.775
5.55GlyAsn: 5.55 ± 1.218
0.0GlyPro: 0.0 ± 0.0
2.148GlyGln: 2.148 ± 0.558
1.969GlyArg: 1.969 ± 0.591
2.685GlySer: 2.685 ± 0.572
3.58GlyThr: 3.58 ± 1.168
4.296GlyVal: 4.296 ± 0.794
1.432GlyTrp: 1.432 ± 0.518
3.58GlyTyr: 3.58 ± 0.943
0.0GlyXaa: 0.0 ± 0.0
His
1.074HisAla: 1.074 ± 0.425
0.358HisCys: 0.358 ± 0.242
0.716HisAsp: 0.716 ± 0.394
1.611HisGlu: 1.611 ± 0.664
1.432HisPhe: 1.432 ± 0.397
0.716HisGly: 0.716 ± 0.359
0.358HisHis: 0.358 ± 0.244
1.79HisIle: 1.79 ± 0.609
1.79HisLys: 1.79 ± 0.598
0.537HisLeu: 0.537 ± 0.418
0.537HisMet: 0.537 ± 0.316
1.432HisAsn: 1.432 ± 0.594
0.358HisPro: 0.358 ± 0.272
0.0HisGln: 0.0 ± 0.0
0.179HisArg: 0.179 ± 0.168
1.79HisSer: 1.79 ± 0.514
0.358HisThr: 0.358 ± 0.246
0.716HisVal: 0.716 ± 0.446
0.179HisTrp: 0.179 ± 0.166
0.895HisTyr: 0.895 ± 0.374
0.0HisXaa: 0.0 ± 0.0
Ile
2.685IleAla: 2.685 ± 0.601
0.895IleCys: 0.895 ± 0.423
5.013IleAsp: 5.013 ± 0.869
7.519IleGlu: 7.519 ± 1.207
2.506IlePhe: 2.506 ± 0.584
6.087IleGly: 6.087 ± 0.773
1.074IleHis: 1.074 ± 0.26
6.087IleIle: 6.087 ± 0.862
8.235IleLys: 8.235 ± 1.116
4.117IleLeu: 4.117 ± 0.683
2.864IleMet: 2.864 ± 0.591
8.235IleAsn: 8.235 ± 1.579
2.148IlePro: 2.148 ± 0.605
2.685IleGln: 2.685 ± 0.665
1.79IleArg: 1.79 ± 0.54
4.117IleSer: 4.117 ± 1.318
5.908IleThr: 5.908 ± 0.795
2.685IleVal: 2.685 ± 0.858
0.358IleTrp: 0.358 ± 0.305
3.759IleTyr: 3.759 ± 0.829
0.0IleXaa: 0.0 ± 0.0
Lys
3.938LysAla: 3.938 ± 0.728
1.253LysCys: 1.253 ± 0.459
6.624LysAsp: 6.624 ± 0.9
7.698LysGlu: 7.698 ± 1.256
3.938LysPhe: 3.938 ± 0.883
7.519LysGly: 7.519 ± 0.793
2.506LysHis: 2.506 ± 0.749
6.087LysIle: 6.087 ± 1.067
7.519LysLys: 7.519 ± 1.657
6.445LysLeu: 6.445 ± 0.961
3.58LysMet: 3.58 ± 0.8
5.908LysAsn: 5.908 ± 0.806
2.148LysPro: 2.148 ± 0.765
3.759LysGln: 3.759 ± 0.909
2.327LysArg: 2.327 ± 0.531
3.222LysSer: 3.222 ± 0.588
5.729LysThr: 5.729 ± 1.534
3.401LysVal: 3.401 ± 0.833
1.253LysTrp: 1.253 ± 0.563
5.729LysTyr: 5.729 ± 1.094
0.0LysXaa: 0.0 ± 0.0
Leu
2.685LeuAla: 2.685 ± 0.591
1.969LeuCys: 1.969 ± 0.426
6.624LeuAsp: 6.624 ± 1.3
4.475LeuGlu: 4.475 ± 0.994
3.043LeuPhe: 3.043 ± 0.737
4.654LeuGly: 4.654 ± 0.937
0.895LeuHis: 0.895 ± 0.467
4.834LeuIle: 4.834 ± 0.856
6.982LeuLys: 6.982 ± 0.976
5.908LeuLeu: 5.908 ± 0.969
1.969LeuMet: 1.969 ± 0.645
7.161LeuAsn: 7.161 ± 1.239
1.611LeuPro: 1.611 ± 0.514
1.969LeuGln: 1.969 ± 0.768
3.043LeuArg: 3.043 ± 0.763
3.222LeuSer: 3.222 ± 1.038
3.58LeuThr: 3.58 ± 0.847
4.296LeuVal: 4.296 ± 1.104
0.537LeuTrp: 0.537 ± 0.274
2.685LeuTyr: 2.685 ± 0.507
0.0LeuXaa: 0.0 ± 0.0
Met
2.327MetAla: 2.327 ± 0.794
0.358MetCys: 0.358 ± 0.241
2.327MetAsp: 2.327 ± 0.692
2.327MetGlu: 2.327 ± 0.517
1.074MetPhe: 1.074 ± 0.378
0.895MetGly: 0.895 ± 0.344
0.0MetHis: 0.0 ± 0.0
2.148MetIle: 2.148 ± 0.422
2.506MetLys: 2.506 ± 0.81
2.506MetLeu: 2.506 ± 0.795
0.179MetMet: 0.179 ± 0.196
2.685MetAsn: 2.685 ± 0.585
1.253MetPro: 1.253 ± 0.468
0.895MetGln: 0.895 ± 0.649
1.074MetArg: 1.074 ± 0.378
2.506MetSer: 2.506 ± 0.698
1.253MetThr: 1.253 ± 0.523
1.611MetVal: 1.611 ± 0.777
0.179MetTrp: 0.179 ± 0.142
2.327MetTyr: 2.327 ± 0.832
0.0MetXaa: 0.0 ± 0.0
Asn
3.938AsnAla: 3.938 ± 0.792
0.895AsnCys: 0.895 ± 0.502
4.296AsnAsp: 4.296 ± 0.934
6.803AsnGlu: 6.803 ± 1.157
2.327AsnPhe: 2.327 ± 0.595
6.087AsnGly: 6.087 ± 1.161
0.895AsnHis: 0.895 ± 0.399
6.982AsnIle: 6.982 ± 0.748
7.161AsnLys: 7.161 ± 1.237
4.654AsnLeu: 4.654 ± 0.926
3.043AsnMet: 3.043 ± 0.779
5.729AsnAsn: 5.729 ± 1.036
2.148AsnPro: 2.148 ± 0.522
1.611AsnGln: 1.611 ± 0.481
3.759AsnArg: 3.759 ± 0.889
5.55AsnSer: 5.55 ± 1.438
5.55AsnThr: 5.55 ± 0.641
3.401AsnVal: 3.401 ± 0.658
0.895AsnTrp: 0.895 ± 0.299
4.654AsnTyr: 4.654 ± 1.307
0.0AsnXaa: 0.0 ± 0.0
Pro
1.432ProAla: 1.432 ± 0.543
0.537ProCys: 0.537 ± 0.326
0.358ProAsp: 0.358 ± 0.186
0.358ProGlu: 0.358 ± 0.203
1.074ProPhe: 1.074 ± 0.388
1.611ProGly: 1.611 ± 0.554
0.0ProHis: 0.0 ± 0.0
2.148ProIle: 2.148 ± 0.443
2.685ProLys: 2.685 ± 0.594
1.79ProLeu: 1.79 ± 0.544
0.537ProMet: 0.537 ± 0.227
1.432ProAsn: 1.432 ± 0.52
0.537ProPro: 0.537 ± 0.241
0.895ProGln: 0.895 ± 0.331
0.179ProArg: 0.179 ± 0.196
1.969ProSer: 1.969 ± 0.518
1.969ProThr: 1.969 ± 0.569
2.506ProVal: 2.506 ± 0.616
0.0ProTrp: 0.0 ± 0.0
3.222ProTyr: 3.222 ± 0.738
0.0ProXaa: 0.0 ± 0.0
Gln
2.327GlnAla: 2.327 ± 0.847
0.716GlnCys: 0.716 ± 0.289
0.895GlnAsp: 0.895 ± 0.437
1.253GlnGlu: 1.253 ± 0.396
1.79GlnPhe: 1.79 ± 0.663
2.506GlnGly: 2.506 ± 0.605
0.0GlnHis: 0.0 ± 0.0
1.969GlnIle: 1.969 ± 0.805
2.327GlnLys: 2.327 ± 0.822
1.253GlnLeu: 1.253 ± 0.518
0.895GlnMet: 0.895 ± 0.316
2.327GlnAsn: 2.327 ± 0.755
0.716GlnPro: 0.716 ± 0.494
0.716GlnGln: 0.716 ± 0.465
1.253GlnArg: 1.253 ± 0.543
2.327GlnSer: 2.327 ± 0.634
1.79GlnThr: 1.79 ± 0.461
1.253GlnVal: 1.253 ± 0.523
0.0GlnTrp: 0.0 ± 0.0
1.611GlnTyr: 1.611 ± 0.511
0.0GlnXaa: 0.0 ± 0.0
Arg
0.716ArgAla: 0.716 ± 0.338
0.895ArgCys: 0.895 ± 0.346
1.074ArgAsp: 1.074 ± 0.401
3.58ArgGlu: 3.58 ± 0.632
1.074ArgPhe: 1.074 ± 0.492
2.506ArgGly: 2.506 ± 0.767
1.074ArgHis: 1.074 ± 0.429
3.222ArgIle: 3.222 ± 0.692
3.222ArgLys: 3.222 ± 1.103
3.043ArgLeu: 3.043 ± 0.817
0.358ArgMet: 0.358 ± 0.25
2.685ArgAsn: 2.685 ± 0.686
0.895ArgPro: 0.895 ± 0.315
0.537ArgGln: 0.537 ± 0.321
2.148ArgArg: 2.148 ± 0.717
0.895ArgSer: 0.895 ± 0.303
3.222ArgThr: 3.222 ± 0.671
1.969ArgVal: 1.969 ± 0.74
1.074ArgTrp: 1.074 ± 0.381
1.79ArgTyr: 1.79 ± 0.46
0.0ArgXaa: 0.0 ± 0.0
Ser
2.506SerAla: 2.506 ± 1.057
1.074SerCys: 1.074 ± 0.433
2.685SerAsp: 2.685 ± 0.862
3.043SerGlu: 3.043 ± 0.764
1.611SerPhe: 1.611 ± 0.561
2.685SerGly: 2.685 ± 0.685
0.895SerHis: 0.895 ± 0.351
4.834SerIle: 4.834 ± 0.878
5.013SerLys: 5.013 ± 0.798
3.222SerLeu: 3.222 ± 0.702
1.969SerMet: 1.969 ± 0.723
4.117SerAsn: 4.117 ± 0.946
0.895SerPro: 0.895 ± 0.37
2.327SerGln: 2.327 ± 0.404
1.611SerArg: 1.611 ± 0.579
4.834SerSer: 4.834 ± 1.343
2.685SerThr: 2.685 ± 0.717
3.401SerVal: 3.401 ± 0.782
1.074SerTrp: 1.074 ± 0.447
2.506SerTyr: 2.506 ± 0.71
0.0SerXaa: 0.0 ± 0.0
Thr
1.79ThrAla: 1.79 ± 0.531
0.716ThrCys: 0.716 ± 0.322
4.654ThrAsp: 4.654 ± 0.863
5.013ThrGlu: 5.013 ± 1.025
1.79ThrPhe: 1.79 ± 0.513
6.266ThrGly: 6.266 ± 0.985
0.895ThrHis: 0.895 ± 0.373
4.834ThrIle: 4.834 ± 0.776
4.654ThrLys: 4.654 ± 1.098
4.296ThrLeu: 4.296 ± 0.886
1.253ThrMet: 1.253 ± 0.606
3.759ThrAsn: 3.759 ± 0.546
1.969ThrPro: 1.969 ± 0.793
1.79ThrGln: 1.79 ± 0.383
1.432ThrArg: 1.432 ± 0.513
3.222ThrSer: 3.222 ± 0.663
3.938ThrThr: 3.938 ± 1.258
3.759ThrVal: 3.759 ± 0.661
0.358ThrTrp: 0.358 ± 0.205
2.864ThrTyr: 2.864 ± 0.869
0.0ThrXaa: 0.0 ± 0.0
Val
1.074ValAla: 1.074 ± 0.448
0.716ValCys: 0.716 ± 0.336
4.296ValAsp: 4.296 ± 0.938
4.475ValGlu: 4.475 ± 0.736
3.222ValPhe: 3.222 ± 0.762
3.222ValGly: 3.222 ± 0.966
1.074ValHis: 1.074 ± 0.383
2.685ValIle: 2.685 ± 0.693
4.296ValLys: 4.296 ± 0.671
4.117ValLeu: 4.117 ± 0.703
1.074ValMet: 1.074 ± 0.371
7.161ValAsn: 7.161 ± 1.17
1.253ValPro: 1.253 ± 0.381
1.253ValGln: 1.253 ± 0.406
2.685ValArg: 2.685 ± 0.778
2.864ValSer: 2.864 ± 0.818
3.401ValThr: 3.401 ± 0.733
2.327ValVal: 2.327 ± 0.448
0.537ValTrp: 0.537 ± 0.341
3.759ValTyr: 3.759 ± 0.822
0.0ValXaa: 0.0 ± 0.0
Trp
0.179TrpAla: 0.179 ± 0.196
0.179TrpCys: 0.179 ± 0.177
0.895TrpAsp: 0.895 ± 0.497
0.895TrpGlu: 0.895 ± 0.346
0.179TrpPhe: 0.179 ± 0.196
0.358TrpGly: 0.358 ± 0.332
0.358TrpHis: 0.358 ± 0.279
1.074TrpIle: 1.074 ± 0.333
0.537TrpLys: 0.537 ± 0.328
1.969TrpLeu: 1.969 ± 0.529
0.0TrpMet: 0.0 ± 0.0
1.253TrpAsn: 1.253 ± 0.429
0.0TrpPro: 0.0 ± 0.0
0.358TrpGln: 0.358 ± 0.228
0.716TrpArg: 0.716 ± 0.325
0.537TrpSer: 0.537 ± 0.247
0.358TrpThr: 0.358 ± 0.214
0.895TrpVal: 0.895 ± 0.461
0.0TrpTrp: 0.0 ± 0.0
0.537TrpTyr: 0.537 ± 0.262
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.148TyrAla: 2.148 ± 0.658
1.074TyrCys: 1.074 ± 0.481
3.58TyrAsp: 3.58 ± 0.632
3.401TyrGlu: 3.401 ± 0.757
3.759TyrPhe: 3.759 ± 0.598
3.58TyrGly: 3.58 ± 0.862
0.895TyrHis: 0.895 ± 0.306
3.222TyrIle: 3.222 ± 0.75
3.759TyrLys: 3.759 ± 0.821
3.58TyrLeu: 3.58 ± 0.496
1.432TyrMet: 1.432 ± 0.548
4.296TyrAsn: 4.296 ± 0.709
2.148TyrPro: 2.148 ± 0.551
1.432TyrGln: 1.432 ± 0.461
2.864TyrArg: 2.864 ± 0.805
2.506TyrSer: 2.506 ± 0.866
3.58TyrThr: 3.58 ± 0.72
3.222TyrVal: 3.222 ± 0.504
0.358TyrTrp: 0.358 ± 0.25
3.043TyrTyr: 3.043 ± 0.767
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (5587 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski