Streptomyces sp. A1136

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2TJN7|A0A4S2TJN7_9ACTN Sigma-70 family RNA polymerase sigma factor OS=Streptomyces sp. A1136 OX=2563102 GN=E6R62_02175 PE=3 SV=1
MM1 pKa = 7.41TVQQEE6 pKa = 4.51GTSDD10 pKa = 3.22RR11 pKa = 11.84TAGAGEE17 pKa = 4.02AQASLEE23 pKa = 4.05VWIDD27 pKa = 3.31QDD29 pKa = 3.99LCTGDD34 pKa = 4.65GICVQYY40 pKa = 10.96APEE43 pKa = 4.18VFEE46 pKa = 5.9LDD48 pKa = 3.05IDD50 pKa = 3.63GLAYY54 pKa = 10.39VKK56 pKa = 10.61SAEE59 pKa = 4.63DD60 pKa = 4.25EE61 pKa = 4.29LLQDD65 pKa = 4.56PGATTPVPLTLLRR78 pKa = 11.84DD79 pKa = 3.74VADD82 pKa = 3.98SAKK85 pKa = 9.94EE86 pKa = 3.91CPGDD90 pKa = 4.18CIHH93 pKa = 6.42VRR95 pKa = 11.84RR96 pKa = 11.84VSDD99 pKa = 3.56GVEE102 pKa = 4.16VYY104 pKa = 10.93GPDD107 pKa = 3.7AEE109 pKa = 4.31

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2T8T7|A0A4S2T8T7_9ACTN Phytoene desaturase OS=Streptomyces sp. A1136 OX=2563102 GN=crtI PE=3 SV=1
MM1 pKa = 7.41TPRR4 pKa = 11.84PVTPWPLGPRR14 pKa = 11.84SRR16 pKa = 11.84PGPRR20 pKa = 11.84PPGPPPTQSRR30 pKa = 11.84PRR32 pKa = 11.84TLRR35 pKa = 11.84AGGSVSSRR43 pKa = 11.84GGVAGSRR50 pKa = 11.84GRR52 pKa = 11.84GAAPAGRR59 pKa = 11.84GRR61 pKa = 11.84LGVPSIRR68 pKa = 11.84ANTT71 pKa = 3.52

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7243

0

7243

2198633

21

3683

303.6

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.947 ± 0.043

0.822 ± 0.008

5.799 ± 0.022

5.667 ± 0.028

2.71 ± 0.014

9.543 ± 0.028

2.297 ± 0.015

3.157 ± 0.021

2.187 ± 0.026

10.286 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.012

1.757 ± 0.017

6.294 ± 0.027

2.711 ± 0.018

8.121 ± 0.034

4.952 ± 0.02

6.032 ± 0.03

8.351 ± 0.026

1.518 ± 0.012

2.087 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski