Amino acid dipepetide frequency for Lactococcus phage P087

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.288AlaAla: 2.288 ± 0.533
0.545AlaCys: 0.545 ± 0.156
2.887AlaAsp: 2.887 ± 0.356
3.813AlaGlu: 3.813 ± 0.458
2.288AlaPhe: 2.288 ± 0.481
3.758AlaGly: 3.758 ± 0.801
0.871AlaHis: 0.871 ± 0.254
4.793AlaIle: 4.793 ± 0.889
5.501AlaLys: 5.501 ± 0.516
6.046AlaLeu: 6.046 ± 0.69
1.688AlaMet: 1.688 ± 0.266
3.976AlaAsn: 3.976 ± 0.415
1.471AlaPro: 1.471 ± 0.277
2.56AlaGln: 2.56 ± 0.597
1.688AlaArg: 1.688 ± 0.332
4.303AlaSer: 4.303 ± 0.701
4.139AlaThr: 4.139 ± 0.455
4.739AlaVal: 4.739 ± 0.502
0.708AlaTrp: 0.708 ± 0.188
2.451AlaTyr: 2.451 ± 0.391
0.0AlaXaa: 0.0 ± 0.0
Cys
0.436CysAla: 0.436 ± 0.149
0.163CysCys: 0.163 ± 0.087
1.198CysAsp: 1.198 ± 0.288
0.926CysGlu: 0.926 ± 0.281
0.49CysPhe: 0.49 ± 0.156
0.926CysGly: 0.926 ± 0.247
0.272CysHis: 0.272 ± 0.127
0.654CysIle: 0.654 ± 0.202
0.763CysLys: 0.763 ± 0.176
0.871CysLeu: 0.871 ± 0.246
0.436CysMet: 0.436 ± 0.142
0.817CysAsn: 0.817 ± 0.223
0.381CysPro: 0.381 ± 0.143
0.327CysGln: 0.327 ± 0.126
0.272CysArg: 0.272 ± 0.135
0.545CysSer: 0.545 ± 0.213
0.272CysThr: 0.272 ± 0.127
0.871CysVal: 0.871 ± 0.225
0.218CysTrp: 0.218 ± 0.102
0.327CysTyr: 0.327 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
2.179AspAla: 2.179 ± 0.419
1.089AspCys: 1.089 ± 0.265
4.085AspAsp: 4.085 ± 0.52
4.575AspGlu: 4.575 ± 0.667
2.996AspPhe: 2.996 ± 0.41
4.357AspGly: 4.357 ± 0.533
0.871AspHis: 0.871 ± 0.249
5.283AspIle: 5.283 ± 0.631
5.065AspLys: 5.065 ± 0.46
6.373AspLeu: 6.373 ± 0.643
2.179AspMet: 2.179 ± 0.338
3.486AspAsn: 3.486 ± 0.444
1.307AspPro: 1.307 ± 0.235
1.362AspGln: 1.362 ± 0.296
1.906AspArg: 1.906 ± 0.313
4.684AspSer: 4.684 ± 0.583
4.248AspThr: 4.248 ± 0.427
4.085AspVal: 4.085 ± 0.411
1.198AspTrp: 1.198 ± 0.291
3.377AspTyr: 3.377 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
4.357GluAla: 4.357 ± 0.662
0.926GluCys: 0.926 ± 0.298
4.031GluAsp: 4.031 ± 0.518
5.229GluGlu: 5.229 ± 0.879
3.758GluPhe: 3.758 ± 0.586
3.159GluGly: 3.159 ± 0.386
1.58GluHis: 1.58 ± 0.327
4.357GluIle: 4.357 ± 0.524
3.268GluLys: 3.268 ± 0.469
7.081GluLeu: 7.081 ± 0.838
1.852GluMet: 1.852 ± 0.339
2.669GluAsn: 2.669 ± 0.378
2.07GluPro: 2.07 ± 0.4
2.887GluGln: 2.887 ± 0.367
3.377GluArg: 3.377 ± 0.558
3.595GluSer: 3.595 ± 0.466
4.357GluThr: 4.357 ± 0.56
5.392GluVal: 5.392 ± 0.556
0.708GluTrp: 0.708 ± 0.208
3.54GluTyr: 3.54 ± 0.562
0.0GluXaa: 0.0 ± 0.0
Phe
2.451PheAla: 2.451 ± 0.398
0.436PheCys: 0.436 ± 0.156
3.54PheAsp: 3.54 ± 0.418
2.614PheGlu: 2.614 ± 0.45
1.144PhePhe: 1.144 ± 0.283
2.941PheGly: 2.941 ± 0.426
0.272PheHis: 0.272 ± 0.121
3.377PheIle: 3.377 ± 0.485
2.832PheLys: 2.832 ± 0.346
2.669PheLeu: 2.669 ± 0.459
0.926PheMet: 0.926 ± 0.231
3.05PheAsn: 3.05 ± 0.445
0.871PhePro: 0.871 ± 0.282
1.471PheGln: 1.471 ± 0.271
0.926PheArg: 0.926 ± 0.205
3.214PheSer: 3.214 ± 0.492
3.322PheThr: 3.322 ± 0.361
2.56PheVal: 2.56 ± 0.383
0.272PheTrp: 0.272 ± 0.114
1.688PheTyr: 1.688 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
4.466GlyAla: 4.466 ± 0.864
0.599GlyCys: 0.599 ± 0.19
3.377GlyAsp: 3.377 ± 0.44
3.54GlyGlu: 3.54 ± 0.515
3.214GlyPhe: 3.214 ± 0.389
5.392GlyGly: 5.392 ± 1.008
0.817GlyHis: 0.817 ± 0.183
4.684GlyIle: 4.684 ± 0.832
4.085GlyLys: 4.085 ± 0.79
4.466GlyLeu: 4.466 ± 0.666
1.797GlyMet: 1.797 ± 0.303
3.486GlyAsn: 3.486 ± 0.525
0.926GlyPro: 0.926 ± 0.19
2.941GlyGln: 2.941 ± 0.454
2.233GlyArg: 2.233 ± 0.308
3.813GlySer: 3.813 ± 0.482
3.976GlyThr: 3.976 ± 0.409
4.248GlyVal: 4.248 ± 0.55
0.708GlyTrp: 0.708 ± 0.176
2.778GlyTyr: 2.778 ± 0.398
0.0GlyXaa: 0.0 ± 0.0
His
1.035HisAla: 1.035 ± 0.249
0.218HisCys: 0.218 ± 0.103
0.98HisAsp: 0.98 ± 0.228
1.089HisGlu: 1.089 ± 0.283
0.599HisPhe: 0.599 ± 0.161
0.599HisGly: 0.599 ± 0.242
0.163HisHis: 0.163 ± 0.107
1.035HisIle: 1.035 ± 0.252
1.144HisLys: 1.144 ± 0.25
1.035HisLeu: 1.035 ± 0.226
0.327HisMet: 0.327 ± 0.141
0.926HisAsn: 0.926 ± 0.268
0.817HisPro: 0.817 ± 0.226
0.49HisGln: 0.49 ± 0.155
0.708HisArg: 0.708 ± 0.227
1.198HisSer: 1.198 ± 0.225
1.416HisThr: 1.416 ± 0.304
0.871HisVal: 0.871 ± 0.181
0.272HisTrp: 0.272 ± 0.148
0.98HisTyr: 0.98 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
4.63IleAla: 4.63 ± 0.477
0.654IleCys: 0.654 ± 0.196
5.556IleAsp: 5.556 ± 0.524
4.521IleGlu: 4.521 ± 0.559
2.669IlePhe: 2.669 ± 0.522
4.085IleGly: 4.085 ± 0.649
0.817IleHis: 0.817 ± 0.175
4.031IleIle: 4.031 ± 0.54
5.61IleLys: 5.61 ± 0.548
5.065IleLeu: 5.065 ± 0.534
1.362IleMet: 1.362 ± 0.222
4.902IleAsn: 4.902 ± 0.453
2.124IlePro: 2.124 ± 0.386
2.723IleGln: 2.723 ± 0.358
2.832IleArg: 2.832 ± 0.342
4.684IleSer: 4.684 ± 0.413
5.12IleThr: 5.12 ± 0.58
4.466IleVal: 4.466 ± 0.532
0.381IleTrp: 0.381 ± 0.126
2.451IleTyr: 2.451 ± 0.379
0.0IleXaa: 0.0 ± 0.0
Lys
4.63LysAla: 4.63 ± 0.762
0.436LysCys: 0.436 ± 0.154
4.847LysAsp: 4.847 ± 0.485
6.536LysGlu: 6.536 ± 0.689
2.996LysPhe: 2.996 ± 0.381
3.595LysGly: 3.595 ± 0.478
1.144LysHis: 1.144 ± 0.317
3.922LysIle: 3.922 ± 0.601
4.466LysLys: 4.466 ± 0.698
6.863LysLeu: 6.863 ± 0.761
2.451LysMet: 2.451 ± 0.334
3.976LysAsn: 3.976 ± 0.385
2.288LysPro: 2.288 ± 0.404
2.233LysGln: 2.233 ± 0.393
2.941LysArg: 2.941 ± 0.383
4.357LysSer: 4.357 ± 0.572
4.521LysThr: 4.521 ± 0.473
4.357LysVal: 4.357 ± 0.648
0.98LysTrp: 0.98 ± 0.214
2.832LysTyr: 2.832 ± 0.371
0.0LysXaa: 0.0 ± 0.0
Leu
4.739LeuAla: 4.739 ± 0.562
0.871LeuCys: 0.871 ± 0.237
6.264LeuAsp: 6.264 ± 0.564
7.081LeuGlu: 7.081 ± 0.605
2.56LeuPhe: 2.56 ± 0.318
6.59LeuGly: 6.59 ± 0.899
1.688LeuHis: 1.688 ± 0.306
4.575LeuIle: 4.575 ± 0.501
6.863LeuLys: 6.863 ± 0.627
6.863LeuLeu: 6.863 ± 0.685
2.124LeuMet: 2.124 ± 0.311
6.481LeuAsn: 6.481 ± 0.544
3.268LeuPro: 3.268 ± 0.424
3.05LeuGln: 3.05 ± 0.393
3.159LeuArg: 3.159 ± 0.389
5.773LeuSer: 5.773 ± 0.487
5.773LeuThr: 5.773 ± 0.475
4.956LeuVal: 4.956 ± 0.449
0.98LeuTrp: 0.98 ± 0.3
3.159LeuTyr: 3.159 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
2.07MetAla: 2.07 ± 0.374
0.218MetCys: 0.218 ± 0.109
1.471MetAsp: 1.471 ± 0.295
1.743MetGlu: 1.743 ± 0.321
1.416MetPhe: 1.416 ± 0.285
1.198MetGly: 1.198 ± 0.216
0.163MetHis: 0.163 ± 0.09
1.797MetIle: 1.797 ± 0.286
1.743MetLys: 1.743 ± 0.293
2.342MetLeu: 2.342 ± 0.353
1.035MetMet: 1.035 ± 0.265
1.852MetAsn: 1.852 ± 0.239
0.98MetPro: 0.98 ± 0.233
1.198MetGln: 1.198 ± 0.219
0.599MetArg: 0.599 ± 0.157
2.288MetSer: 2.288 ± 0.277
2.614MetThr: 2.614 ± 0.357
2.233MetVal: 2.233 ± 0.338
0.109MetTrp: 0.109 ± 0.082
0.871MetTyr: 0.871 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
4.793AsnAla: 4.793 ± 0.491
1.035AsnCys: 1.035 ± 0.267
3.649AsnAsp: 3.649 ± 0.487
3.976AsnGlu: 3.976 ± 0.576
1.797AsnPhe: 1.797 ± 0.328
4.575AsnGly: 4.575 ± 0.526
1.144AsnHis: 1.144 ± 0.218
4.793AsnIle: 4.793 ± 0.47
4.575AsnLys: 4.575 ± 0.418
5.338AsnLeu: 5.338 ± 0.703
1.634AsnMet: 1.634 ± 0.263
4.357AsnAsn: 4.357 ± 0.758
3.159AsnPro: 3.159 ± 0.488
2.451AsnGln: 2.451 ± 0.359
2.397AsnArg: 2.397 ± 0.365
2.669AsnSer: 2.669 ± 0.335
4.521AsnThr: 4.521 ± 0.509
3.377AsnVal: 3.377 ± 0.468
0.654AsnTrp: 0.654 ± 0.163
2.015AsnTyr: 2.015 ± 0.375
0.0AsnXaa: 0.0 ± 0.0
Pro
1.797ProAla: 1.797 ± 0.399
0.327ProCys: 0.327 ± 0.118
2.015ProAsp: 2.015 ± 0.313
2.124ProGlu: 2.124 ± 0.414
1.253ProPhe: 1.253 ± 0.279
1.471ProGly: 1.471 ± 0.263
0.654ProHis: 0.654 ± 0.161
2.451ProIle: 2.451 ± 0.378
2.778ProLys: 2.778 ± 0.454
2.397ProLeu: 2.397 ± 0.3
0.599ProMet: 0.599 ± 0.176
2.233ProAsn: 2.233 ± 0.289
0.817ProPro: 0.817 ± 0.196
1.362ProGln: 1.362 ± 0.279
1.253ProArg: 1.253 ± 0.312
1.797ProSer: 1.797 ± 0.279
3.649ProThr: 3.649 ± 0.527
2.614ProVal: 2.614 ± 0.377
0.218ProTrp: 0.218 ± 0.107
1.035ProTyr: 1.035 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
3.268GlnAla: 3.268 ± 0.624
0.436GlnCys: 0.436 ± 0.146
2.015GlnAsp: 2.015 ± 0.383
2.397GlnGlu: 2.397 ± 0.408
1.961GlnPhe: 1.961 ± 0.413
1.743GlnGly: 1.743 ± 0.322
0.599GlnHis: 0.599 ± 0.173
2.505GlnIle: 2.505 ± 0.406
1.852GlnLys: 1.852 ± 0.318
3.922GlnLeu: 3.922 ± 0.4
1.144GlnMet: 1.144 ± 0.271
2.124GlnAsn: 2.124 ± 0.368
1.416GlnPro: 1.416 ± 0.338
1.634GlnGln: 1.634 ± 0.326
1.743GlnArg: 1.743 ± 0.346
2.56GlnSer: 2.56 ± 0.368
2.397GlnThr: 2.397 ± 0.438
2.996GlnVal: 2.996 ± 0.46
0.49GlnTrp: 0.49 ± 0.205
1.961GlnTyr: 1.961 ± 0.325
0.0GlnXaa: 0.0 ± 0.0
Arg
1.634ArgAla: 1.634 ± 0.268
0.218ArgCys: 0.218 ± 0.097
2.07ArgAsp: 2.07 ± 0.381
2.015ArgGlu: 2.015 ± 0.425
1.906ArgPhe: 1.906 ± 0.352
1.688ArgGly: 1.688 ± 0.315
0.272ArgHis: 0.272 ± 0.121
2.179ArgIle: 2.179 ± 0.408
2.397ArgLys: 2.397 ± 0.476
4.466ArgLeu: 4.466 ± 0.493
0.926ArgMet: 0.926 ± 0.225
2.07ArgAsn: 2.07 ± 0.411
1.089ArgPro: 1.089 ± 0.249
2.451ArgGln: 2.451 ± 0.344
1.852ArgArg: 1.852 ± 0.304
2.179ArgSer: 2.179 ± 0.434
2.342ArgThr: 2.342 ± 0.36
2.233ArgVal: 2.233 ± 0.339
0.381ArgTrp: 0.381 ± 0.138
1.634ArgTyr: 1.634 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
4.248SerAla: 4.248 ± 0.622
0.926SerCys: 0.926 ± 0.242
4.031SerAsp: 4.031 ± 0.518
3.976SerGlu: 3.976 ± 0.478
2.778SerPhe: 2.778 ± 0.351
3.813SerGly: 3.813 ± 0.451
1.198SerHis: 1.198 ± 0.338
5.12SerIle: 5.12 ± 0.663
4.194SerLys: 4.194 ± 0.486
5.338SerLeu: 5.338 ± 0.581
1.961SerMet: 1.961 ± 0.251
3.486SerAsn: 3.486 ± 0.323
1.58SerPro: 1.58 ± 0.26
2.887SerGln: 2.887 ± 0.411
1.852SerArg: 1.852 ± 0.349
4.194SerSer: 4.194 ± 0.791
3.976SerThr: 3.976 ± 0.521
4.031SerVal: 4.031 ± 0.503
0.926SerTrp: 0.926 ± 0.365
2.56SerTyr: 2.56 ± 0.399
0.0SerXaa: 0.0 ± 0.0
Thr
3.867ThrAla: 3.867 ± 0.454
0.926ThrCys: 0.926 ± 0.18
4.412ThrAsp: 4.412 ± 0.575
3.976ThrGlu: 3.976 ± 0.513
2.56ThrPhe: 2.56 ± 0.295
4.847ThrGly: 4.847 ± 0.701
1.144ThrHis: 1.144 ± 0.232
4.521ThrIle: 4.521 ± 0.469
4.847ThrLys: 4.847 ± 0.546
6.318ThrLeu: 6.318 ± 0.562
2.179ThrMet: 2.179 ± 0.304
4.466ThrAsn: 4.466 ± 0.532
2.832ThrPro: 2.832 ± 0.385
2.941ThrGln: 2.941 ± 0.468
1.961ThrArg: 1.961 ± 0.371
3.704ThrSer: 3.704 ± 0.452
5.065ThrThr: 5.065 ± 0.65
5.991ThrVal: 5.991 ± 0.682
1.089ThrTrp: 1.089 ± 0.272
2.669ThrTyr: 2.669 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
4.031ValAla: 4.031 ± 0.528
0.436ValCys: 0.436 ± 0.182
4.139ValAsp: 4.139 ± 0.477
4.956ValGlu: 4.956 ± 0.609
1.906ValPhe: 1.906 ± 0.353
4.085ValGly: 4.085 ± 0.524
1.362ValHis: 1.362 ± 0.301
5.12ValIle: 5.12 ± 0.556
5.011ValLys: 5.011 ± 0.479
4.847ValLeu: 4.847 ± 0.494
1.906ValMet: 1.906 ± 0.349
4.63ValAsn: 4.63 ± 0.509
3.758ValPro: 3.758 ± 0.427
1.906ValGln: 1.906 ± 0.325
2.342ValArg: 2.342 ± 0.387
3.867ValSer: 3.867 ± 0.432
5.065ValThr: 5.065 ± 0.559
3.268ValVal: 3.268 ± 0.525
0.545ValTrp: 0.545 ± 0.163
2.614ValTyr: 2.614 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
0.599TrpAla: 0.599 ± 0.217
0.109TrpCys: 0.109 ± 0.073
0.436TrpAsp: 0.436 ± 0.14
0.763TrpGlu: 0.763 ± 0.162
0.49TrpPhe: 0.49 ± 0.157
0.49TrpGly: 0.49 ± 0.163
0.272TrpHis: 0.272 ± 0.119
0.817TrpIle: 0.817 ± 0.231
0.871TrpLys: 0.871 ± 0.264
1.198TrpLeu: 1.198 ± 0.262
0.327TrpMet: 0.327 ± 0.132
0.98TrpAsn: 0.98 ± 0.225
0.0TrpPro: 0.0 ± 0.0
0.49TrpGln: 0.49 ± 0.146
0.381TrpArg: 0.381 ± 0.144
0.98TrpSer: 0.98 ± 0.202
0.817TrpThr: 0.817 ± 0.226
0.381TrpVal: 0.381 ± 0.138
0.109TrpTrp: 0.109 ± 0.075
0.871TrpTyr: 0.871 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.996TyrAla: 2.996 ± 0.321
0.599TyrCys: 0.599 ± 0.219
3.704TyrAsp: 3.704 ± 0.39
2.342TyrGlu: 2.342 ± 0.403
1.797TyrPhe: 1.797 ± 0.382
2.233TyrGly: 2.233 ± 0.438
0.599TyrHis: 0.599 ± 0.161
2.669TyrIle: 2.669 ± 0.367
2.505TyrLys: 2.505 ± 0.387
3.377TyrLeu: 3.377 ± 0.479
1.035TyrMet: 1.035 ± 0.239
2.941TyrAsn: 2.941 ± 0.409
1.688TyrPro: 1.688 ± 0.369
1.797TyrGln: 1.797 ± 0.33
1.58TyrArg: 1.58 ± 0.285
2.669TyrSer: 2.669 ± 0.469
2.723TyrThr: 2.723 ± 0.393
2.124TyrVal: 2.124 ± 0.246
0.436TyrTrp: 0.436 ± 0.175
2.288TyrTyr: 2.288 ± 0.361
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (18361 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski