Pseudogymnoascus sp. VKM F-3775

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093YLA5|A0A093YLA5_9PEZI ANK_REP_REGION domain-containing protein OS=Pseudogymnoascus sp. VKM F-3775 OX=1420901 GN=V491_09064 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.62SHH4 pKa = 6.21NLKK7 pKa = 10.47VPLDD11 pKa = 3.87IDD13 pKa = 4.6DD14 pKa = 4.44SALLLADD21 pKa = 4.52FQCDD25 pKa = 3.2EE26 pKa = 4.45ATVGTSFDD34 pKa = 3.34EE35 pKa = 4.93SIAPFTFSSNIDD47 pKa = 2.74GRR49 pKa = 11.84YY50 pKa = 8.87QLNTASLFDD59 pKa = 3.67TEE61 pKa = 4.97YY62 pKa = 10.7IDD64 pKa = 4.91AALLEE69 pKa = 4.41AQNPVSTNNFLNNYY83 pKa = 7.93CSPAIQDD90 pKa = 3.82TQNDD94 pKa = 4.24LSPFHH99 pKa = 6.65PAWHH103 pKa = 6.17TPAANFEE110 pKa = 4.12EE111 pKa = 4.4QQQNFQSQQGVPDD124 pKa = 3.86GAFPNALNTSSHH136 pKa = 7.09DD137 pKa = 3.38GSSFDD142 pKa = 5.34WPEE145 pKa = 3.34AWQGGQGSLTTLDD158 pKa = 4.34HH159 pKa = 6.92IGNEE163 pKa = 4.23PTTSLAFSQKK173 pKa = 10.62EE174 pKa = 4.11EE175 pKa = 4.14MEE177 pKa = 4.39HH178 pKa = 6.92FGG180 pKa = 4.59

Molecular weight: 19.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093Z995|A0A093Z995_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-3775 OX=1420901 GN=V491_06322 PE=4 SV=1
MM1 pKa = 7.03STPGAPPAGGGRR13 pKa = 11.84GGRR16 pKa = 11.84HH17 pKa = 3.23RR18 pKa = 11.84HH19 pKa = 3.88YY20 pKa = 10.38RR21 pKa = 11.84RR22 pKa = 11.84PRR24 pKa = 11.84RR25 pKa = 11.84GGAQNGAAPAPRR37 pKa = 11.84APQVTPAQPTPGSATAVPPAPVAPQAAPGSNTNPSRR73 pKa = 11.84RR74 pKa = 11.84NNGRR78 pKa = 11.84SNRR81 pKa = 11.84GGAGRR86 pKa = 11.84QGQRR90 pKa = 11.84GGAPRR95 pKa = 11.84HH96 pKa = 5.2VMGGG100 pKa = 3.33

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9472

0

9472

4032644

12

4963

425.7

47.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.551 ± 0.021

1.21 ± 0.01

5.728 ± 0.019

6.317 ± 0.025

3.741 ± 0.015

7.044 ± 0.022

2.233 ± 0.011

5.369 ± 0.018

5.083 ± 0.022

8.879 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.011

3.876 ± 0.014

5.614 ± 0.021

3.857 ± 0.018

5.688 ± 0.02

8.083 ± 0.025

6.021 ± 0.016

6.179 ± 0.017

1.475 ± 0.009

2.856 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski