Flavobacteriaceae bacterium Ap0902

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N8UHL2|A0A6N8UHL2_9FLAO 50S ribosomal protein L20 OS=Flavobacteriaceae bacterium Ap0902 OX=2684397 GN=rplT PE=3 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84ITFTLLSAFCAILIQAQTVQDD23 pKa = 3.83AQKK26 pKa = 10.73KK27 pKa = 8.33IDD29 pKa = 3.68KK30 pKa = 10.88VYY32 pKa = 11.28DD33 pKa = 3.63NLNTSPYY40 pKa = 10.67LLQTEE45 pKa = 4.3QFKK48 pKa = 10.52IDD50 pKa = 4.13HH51 pKa = 6.25LHH53 pKa = 6.21SNPKK57 pKa = 9.75KK58 pKa = 10.63ALLNIQNPARR68 pKa = 11.84TEE70 pKa = 3.93QNIFTPLEE78 pKa = 4.34NKK80 pKa = 9.92PGDD83 pKa = 3.61YY84 pKa = 9.58WYY86 pKa = 11.43SSMDD90 pKa = 3.07WADD93 pKa = 3.2YY94 pKa = 11.08DD95 pKa = 4.15QDD97 pKa = 4.11GDD99 pKa = 4.21LDD101 pKa = 4.24LLISGAHH108 pKa = 5.65PRR110 pKa = 11.84EE111 pKa = 4.15GAPYY115 pKa = 10.06DD116 pKa = 3.72AGASTTHH123 pKa = 6.57VYY125 pKa = 11.26DD126 pKa = 3.54NDD128 pKa = 4.05GNGNLSLNTDD138 pKa = 3.94IILQGVHH145 pKa = 6.5ASDD148 pKa = 3.82VHH150 pKa = 6.59WLDD153 pKa = 3.42FDD155 pKa = 4.54YY156 pKa = 11.4DD157 pKa = 3.73GDD159 pKa = 4.38LDD161 pKa = 3.82IVMMGIDD168 pKa = 4.21YY169 pKa = 7.54DD170 pKa = 3.98TAFNMSALYY179 pKa = 10.21EE180 pKa = 4.31NNNGTFTAIEE190 pKa = 4.22VGTNGASWGGISIGDD205 pKa = 3.73YY206 pKa = 11.39DD207 pKa = 4.74NDD209 pKa = 3.84GDD211 pKa = 5.95LDD213 pKa = 3.75MLMTGWNVDD222 pKa = 4.39DD223 pKa = 4.95IDD225 pKa = 3.88NGYY228 pKa = 11.24ASTFLWEE235 pKa = 4.46NNDD238 pKa = 3.16GTFVRR243 pKa = 11.84NNISAQVPGVFNGNIAWVDD262 pKa = 3.0IDD264 pKa = 4.83NDD266 pKa = 3.42MDD268 pKa = 4.53LDD270 pKa = 3.96IVVSPAYY277 pKa = 10.17GVDD280 pKa = 3.49VQNLRR285 pKa = 11.84IYY287 pKa = 10.5KK288 pKa = 9.82RR289 pKa = 11.84EE290 pKa = 3.62NDD292 pKa = 3.8IYY294 pKa = 11.27TLTQKK299 pKa = 10.36VNNSSSYY306 pKa = 11.85AMFALGDD313 pKa = 3.78YY314 pKa = 10.94NNDD317 pKa = 3.03GFVDD321 pKa = 3.7IAVQTTDD328 pKa = 3.92DD329 pKa = 4.43DD330 pKa = 4.76YY331 pKa = 11.96NNFVDD336 pKa = 4.85ILKK339 pKa = 10.54NNQGDD344 pKa = 3.94FEE346 pKa = 4.63YY347 pKa = 11.21VEE349 pKa = 4.61SLPSVSSNGAVTPLAFGDD367 pKa = 3.85YY368 pKa = 11.02DD369 pKa = 4.16NDD371 pKa = 3.76GDD373 pKa = 5.38LDD375 pKa = 4.18LAVAGLDD382 pKa = 3.11SDD384 pKa = 4.73YY385 pKa = 11.3IGVSLLYY392 pKa = 10.54QNSDD396 pKa = 3.17NQFTIVNDD404 pKa = 3.53TGFDD408 pKa = 3.42KK409 pKa = 11.45VGGHH413 pKa = 5.43SLSWADD419 pKa = 3.7IDD421 pKa = 5.31GDD423 pKa = 4.2LDD425 pKa = 4.61LDD427 pKa = 3.64ILITGFIDD435 pKa = 4.94DD436 pKa = 3.97EE437 pKa = 5.43DD438 pKa = 3.8IGEE441 pKa = 4.37FYY443 pKa = 10.0GTSRR447 pKa = 11.84LYY449 pKa = 11.3KK450 pKa = 10.34NDD452 pKa = 3.11IGSANTAPNPPTTLSTQRR470 pKa = 11.84DD471 pKa = 3.61NGSVVFNWADD481 pKa = 3.16ASDD484 pKa = 3.92EE485 pKa = 4.04QTPTEE490 pKa = 4.06GLQYY494 pKa = 10.49QLQVGKK500 pKa = 10.62SPGGSDD506 pKa = 2.63IANYY510 pKa = 10.24SINNTSWVLNNLSPEE525 pKa = 4.1TTYY528 pKa = 10.67YY529 pKa = 9.94WCVKK533 pKa = 10.56SIDD536 pKa = 3.57TSFVKK541 pKa = 10.26SQCSEE546 pKa = 3.64EE547 pKa = 4.12QMISNLSTEE556 pKa = 4.42EE557 pKa = 3.91VNLDD561 pKa = 3.59KK562 pKa = 11.45NVVRR566 pKa = 11.84LVSNPVEE573 pKa = 3.85NGIIHH578 pKa = 7.24LVTSEE583 pKa = 3.74WGHH586 pKa = 7.42PEE588 pKa = 4.0MQVKK592 pKa = 10.18LYY594 pKa = 10.98DD595 pKa = 3.64MKK597 pKa = 11.31GGLILNKK604 pKa = 9.33TFNDD608 pKa = 2.76IRR610 pKa = 11.84NIIDD614 pKa = 3.47IPVHH618 pKa = 5.77HH619 pKa = 7.44LSTGKK624 pKa = 10.86YY625 pKa = 8.29ILQYY629 pKa = 10.83QNQTDD634 pKa = 3.78TGALPIIIKK643 pKa = 9.42QQ644 pKa = 3.3

Molecular weight: 71.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N8UGE4|A0A6N8UGE4_9FLAO 50S ribosomal protein L4 OS=Flavobacteriaceae bacterium Ap0902 OX=2684397 GN=rplD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.86KK12 pKa = 10.03RR13 pKa = 11.84NKK15 pKa = 9.59HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.1RR21 pKa = 11.84MSTKK25 pKa = 9.7NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.65GRR39 pKa = 11.84KK40 pKa = 8.69NISVSTVRR48 pKa = 11.84AKK50 pKa = 10.57RR51 pKa = 3.36

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2350

0

2350

768563

38

2351

327.0

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.326 ± 0.052

0.617 ± 0.015

5.85 ± 0.037

6.979 ± 0.055

5.028 ± 0.041

6.373 ± 0.056

1.895 ± 0.023

8.125 ± 0.051

7.521 ± 0.059

9.276 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.025

6.086 ± 0.048

3.462 ± 0.028

3.702 ± 0.028

3.623 ± 0.03

6.002 ± 0.035

5.321 ± 0.031

5.996 ± 0.036

1.083 ± 0.02

4.301 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski