Amino acid dipepetide frequency for Fusobacterium phage Funu1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.139AlaAla: 4.139 ± 0.789
0.169AlaCys: 0.169 ± 0.118
2.28AlaAsp: 2.28 ± 0.381
3.463AlaGlu: 3.463 ± 0.608
2.196AlaPhe: 2.196 ± 0.447
4.561AlaGly: 4.561 ± 0.675
0.169AlaHis: 0.169 ± 0.127
4.307AlaIle: 4.307 ± 0.663
4.645AlaLys: 4.645 ± 0.456
4.223AlaLeu: 4.223 ± 0.569
1.267AlaMet: 1.267 ± 0.336
3.294AlaAsn: 3.294 ± 0.509
1.182AlaPro: 1.182 ± 0.336
1.267AlaGln: 1.267 ± 0.365
1.098AlaArg: 1.098 ± 0.375
3.463AlaSer: 3.463 ± 0.604
2.787AlaThr: 2.787 ± 0.578
2.787AlaVal: 2.787 ± 0.421
0.929AlaTrp: 0.929 ± 0.176
1.436AlaTyr: 1.436 ± 0.371
0.0AlaXaa: 0.0 ± 0.0
Cys
0.422CysAla: 0.422 ± 0.202
0.084CysCys: 0.084 ± 0.097
0.338CysAsp: 0.338 ± 0.184
0.845CysGlu: 0.845 ± 0.278
0.338CysPhe: 0.338 ± 0.173
0.76CysGly: 0.76 ± 0.309
0.169CysHis: 0.169 ± 0.104
0.591CysIle: 0.591 ± 0.243
1.52CysLys: 1.52 ± 0.396
0.76CysLeu: 0.76 ± 0.259
0.084CysMet: 0.084 ± 0.097
0.76CysAsn: 0.76 ± 0.275
0.507CysPro: 0.507 ± 0.247
0.338CysGln: 0.338 ± 0.146
0.338CysArg: 0.338 ± 0.206
0.76CysSer: 0.76 ± 0.236
0.422CysThr: 0.422 ± 0.189
0.507CysVal: 0.507 ± 0.186
0.0CysTrp: 0.0 ± 0.0
0.169CysTyr: 0.169 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
2.534AspAla: 2.534 ± 0.368
0.422AspCys: 0.422 ± 0.176
2.956AspAsp: 2.956 ± 0.549
4.73AspGlu: 4.73 ± 0.712
4.307AspPhe: 4.307 ± 0.598
3.041AspGly: 3.041 ± 0.764
0.253AspHis: 0.253 ± 0.139
5.828AspIle: 5.828 ± 0.808
4.307AspLys: 4.307 ± 0.638
5.743AspLeu: 5.743 ± 0.529
1.182AspMet: 1.182 ± 0.371
2.956AspAsn: 2.956 ± 0.511
1.52AspPro: 1.52 ± 0.453
0.422AspGln: 0.422 ± 0.178
1.436AspArg: 1.436 ± 0.439
2.534AspSer: 2.534 ± 0.45
2.872AspThr: 2.872 ± 0.419
3.378AspVal: 3.378 ± 0.556
0.169AspTrp: 0.169 ± 0.128
2.534AspTyr: 2.534 ± 0.482
0.0AspXaa: 0.0 ± 0.0
Glu
4.307GluAla: 4.307 ± 0.547
0.676GluCys: 0.676 ± 0.27
5.152GluAsp: 5.152 ± 0.634
9.122GluGlu: 9.122 ± 0.96
2.956GluPhe: 2.956 ± 0.478
2.111GluGly: 2.111 ± 0.334
1.182GluHis: 1.182 ± 0.38
11.74GluIle: 11.74 ± 1.177
13.26GluLys: 13.26 ± 1.375
8.277GluLeu: 8.277 ± 0.838
2.111GluMet: 2.111 ± 0.416
7.095GluAsn: 7.095 ± 0.713
1.52GluPro: 1.52 ± 0.329
2.28GluGln: 2.28 ± 0.409
3.632GluArg: 3.632 ± 0.656
4.054GluSer: 4.054 ± 0.541
3.716GluThr: 3.716 ± 0.453
5.405GluVal: 5.405 ± 0.773
1.098GluTrp: 1.098 ± 0.325
3.294GluTyr: 3.294 ± 0.523
0.0GluXaa: 0.0 ± 0.0
Phe
1.098PheAla: 1.098 ± 0.338
0.253PheCys: 0.253 ± 0.131
1.689PheAsp: 1.689 ± 0.324
4.139PheGlu: 4.139 ± 0.576
2.787PhePhe: 2.787 ± 0.43
2.618PheGly: 2.618 ± 0.441
0.253PheHis: 0.253 ± 0.147
3.97PheIle: 3.97 ± 0.523
5.068PheLys: 5.068 ± 0.599
4.307PheLeu: 4.307 ± 0.596
1.267PheMet: 1.267 ± 0.282
3.632PheAsn: 3.632 ± 0.619
2.027PhePro: 2.027 ± 0.531
1.351PheGln: 1.351 ± 0.278
1.267PheArg: 1.267 ± 0.324
4.139PheSer: 4.139 ± 0.525
2.28PheThr: 2.28 ± 0.372
1.605PheVal: 1.605 ± 0.358
0.253PheTrp: 0.253 ± 0.135
1.689PheTyr: 1.689 ± 0.435
0.0PheXaa: 0.0 ± 0.0
Gly
4.054GlyAla: 4.054 ± 0.861
0.253GlyCys: 0.253 ± 0.146
3.125GlyAsp: 3.125 ± 0.52
3.632GlyGlu: 3.632 ± 0.613
2.111GlyPhe: 2.111 ± 0.364
2.534GlyGly: 2.534 ± 0.386
1.014GlyHis: 1.014 ± 0.232
5.743GlyIle: 5.743 ± 0.603
6.926GlyLys: 6.926 ± 0.958
3.885GlyLeu: 3.885 ± 0.5
1.098GlyMet: 1.098 ± 0.34
2.872GlyAsn: 2.872 ± 0.604
1.014GlyPro: 1.014 ± 0.249
1.182GlyGln: 1.182 ± 0.285
2.196GlyArg: 2.196 ± 0.366
1.689GlySer: 1.689 ± 0.404
3.463GlyThr: 3.463 ± 0.611
3.041GlyVal: 3.041 ± 0.464
0.591GlyTrp: 0.591 ± 0.21
2.872GlyTyr: 2.872 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
0.084HisAla: 0.084 ± 0.079
0.422HisCys: 0.422 ± 0.206
0.338HisAsp: 0.338 ± 0.146
0.591HisGlu: 0.591 ± 0.18
0.845HisPhe: 0.845 ± 0.248
0.253HisGly: 0.253 ± 0.15
0.169HisHis: 0.169 ± 0.118
0.338HisIle: 0.338 ± 0.157
0.845HisLys: 0.845 ± 0.284
1.267HisLeu: 1.267 ± 0.374
0.338HisMet: 0.338 ± 0.171
1.098HisAsn: 1.098 ± 0.345
0.422HisPro: 0.422 ± 0.139
0.76HisGln: 0.76 ± 0.239
0.338HisArg: 0.338 ± 0.156
1.014HisSer: 1.014 ± 0.273
0.422HisThr: 0.422 ± 0.189
0.253HisVal: 0.253 ± 0.141
0.0HisTrp: 0.0 ± 0.0
0.422HisTyr: 0.422 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
4.307IleAla: 4.307 ± 0.557
0.929IleCys: 0.929 ± 0.306
5.997IleAsp: 5.997 ± 0.658
10.22IleGlu: 10.22 ± 1.192
4.561IlePhe: 4.561 ± 0.643
4.983IleGly: 4.983 ± 0.982
1.267IleHis: 1.267 ± 0.334
7.77IleIle: 7.77 ± 0.881
10.811IleLys: 10.811 ± 1.1
8.53IleLeu: 8.53 ± 0.636
1.351IleMet: 1.351 ± 0.233
6.841IleAsn: 6.841 ± 0.837
2.787IlePro: 2.787 ± 0.457
2.365IleGln: 2.365 ± 0.489
3.209IleArg: 3.209 ± 0.531
6.166IleSer: 6.166 ± 0.746
5.236IleThr: 5.236 ± 0.662
4.392IleVal: 4.392 ± 0.529
0.845IleTrp: 0.845 ± 0.272
3.885IleTyr: 3.885 ± 0.379
0.0IleXaa: 0.0 ± 0.0
Lys
6.334LysAla: 6.334 ± 0.914
0.929LysCys: 0.929 ± 0.231
5.997LysAsp: 5.997 ± 0.527
12.5LysGlu: 12.5 ± 0.865
4.054LysPhe: 4.054 ± 0.607
5.068LysGly: 5.068 ± 0.689
1.351LysHis: 1.351 ± 0.332
13.176LysIle: 13.176 ± 1.123
13.598LysLys: 13.598 ± 1.273
9.713LysLeu: 9.713 ± 0.886
3.547LysMet: 3.547 ± 0.505
10.895LysAsn: 10.895 ± 0.895
2.111LysPro: 2.111 ± 0.45
2.28LysGln: 2.28 ± 0.401
4.139LysArg: 4.139 ± 0.509
5.321LysSer: 5.321 ± 0.608
5.574LysThr: 5.574 ± 0.528
6.757LysVal: 6.757 ± 0.826
0.929LysTrp: 0.929 ± 0.314
5.912LysTyr: 5.912 ± 0.658
0.0LysXaa: 0.0 ± 0.0
Leu
4.392LeuAla: 4.392 ± 0.733
1.182LeuCys: 1.182 ± 0.358
6.081LeuAsp: 6.081 ± 0.625
9.713LeuGlu: 9.713 ± 1.035
3.547LeuPhe: 3.547 ± 0.466
5.068LeuGly: 5.068 ± 0.524
0.676LeuHis: 0.676 ± 0.214
7.348LeuIle: 7.348 ± 0.692
9.375LeuLys: 9.375 ± 0.977
5.997LeuLeu: 5.997 ± 0.701
1.943LeuMet: 1.943 ± 0.312
5.574LeuAsn: 5.574 ± 0.641
1.689LeuPro: 1.689 ± 0.352
3.041LeuGln: 3.041 ± 0.451
2.618LeuArg: 2.618 ± 0.444
4.899LeuSer: 4.899 ± 0.479
4.645LeuThr: 4.645 ± 0.675
3.378LeuVal: 3.378 ± 0.539
0.676LeuTrp: 0.676 ± 0.286
3.209LeuTyr: 3.209 ± 0.494
0.0LeuXaa: 0.0 ± 0.0
Met
1.098MetAla: 1.098 ± 0.235
0.422MetCys: 0.422 ± 0.192
0.929MetAsp: 0.929 ± 0.277
2.449MetGlu: 2.449 ± 0.41
0.76MetPhe: 0.76 ± 0.184
1.689MetGly: 1.689 ± 0.369
0.253MetHis: 0.253 ± 0.141
1.52MetIle: 1.52 ± 0.291
2.703MetLys: 2.703 ± 0.468
1.436MetLeu: 1.436 ± 0.337
0.338MetMet: 0.338 ± 0.153
2.196MetAsn: 2.196 ± 0.474
0.845MetPro: 0.845 ± 0.227
0.676MetGln: 0.676 ± 0.274
0.591MetArg: 0.591 ± 0.232
1.351MetSer: 1.351 ± 0.349
0.845MetThr: 0.845 ± 0.262
0.845MetVal: 0.845 ± 0.262
0.0MetTrp: 0.0 ± 0.0
0.253MetTyr: 0.253 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
2.111AsnAla: 2.111 ± 0.446
0.845AsnCys: 0.845 ± 0.247
3.209AsnAsp: 3.209 ± 0.54
5.152AsnGlu: 5.152 ± 0.807
4.139AsnPhe: 4.139 ± 0.51
3.801AsnGly: 3.801 ± 0.566
0.845AsnHis: 0.845 ± 0.227
8.024AsnIle: 8.024 ± 0.769
8.446AsnLys: 8.446 ± 1.044
7.01AsnLeu: 7.01 ± 0.785
1.774AsnMet: 1.774 ± 0.352
4.814AsnAsn: 4.814 ± 0.673
1.351AsnPro: 1.351 ± 0.36
2.027AsnGln: 2.027 ± 0.446
1.943AsnArg: 1.943 ± 0.378
4.392AsnSer: 4.392 ± 0.485
2.449AsnThr: 2.449 ± 0.394
2.956AsnVal: 2.956 ± 0.408
1.098AsnTrp: 1.098 ± 0.291
3.378AsnTyr: 3.378 ± 0.462
0.0AsnXaa: 0.0 ± 0.0
Pro
1.098ProAla: 1.098 ± 0.329
0.507ProCys: 0.507 ± 0.205
1.436ProAsp: 1.436 ± 0.323
2.027ProGlu: 2.027 ± 0.342
0.76ProPhe: 0.76 ± 0.244
1.182ProGly: 1.182 ± 0.364
0.422ProHis: 0.422 ± 0.157
2.28ProIle: 2.28 ± 0.503
3.041ProLys: 3.041 ± 0.614
1.436ProLeu: 1.436 ± 0.319
0.422ProMet: 0.422 ± 0.16
1.52ProAsn: 1.52 ± 0.364
0.507ProPro: 0.507 ± 0.173
0.507ProGln: 0.507 ± 0.208
1.014ProArg: 1.014 ± 0.269
1.436ProSer: 1.436 ± 0.343
1.689ProThr: 1.689 ± 0.391
1.605ProVal: 1.605 ± 0.348
0.084ProTrp: 0.084 ± 0.08
1.182ProTyr: 1.182 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
1.182GlnAla: 1.182 ± 0.282
0.169GlnCys: 0.169 ± 0.095
1.351GlnAsp: 1.351 ± 0.405
2.703GlnGlu: 2.703 ± 0.489
0.929GlnPhe: 0.929 ± 0.218
1.774GlnGly: 1.774 ± 0.366
0.0GlnHis: 0.0 ± 0.0
2.027GlnIle: 2.027 ± 0.446
4.307GlnLys: 4.307 ± 0.581
2.956GlnLeu: 2.956 ± 0.394
0.253GlnMet: 0.253 ± 0.128
1.52GlnAsn: 1.52 ± 0.352
0.76GlnPro: 0.76 ± 0.226
0.507GlnGln: 0.507 ± 0.174
1.014GlnArg: 1.014 ± 0.273
1.689GlnSer: 1.689 ± 0.325
1.014GlnThr: 1.014 ± 0.281
1.182GlnVal: 1.182 ± 0.305
0.084GlnTrp: 0.084 ± 0.076
1.182GlnTyr: 1.182 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
1.436ArgAla: 1.436 ± 0.361
0.253ArgCys: 0.253 ± 0.155
1.52ArgAsp: 1.52 ± 0.379
3.716ArgGlu: 3.716 ± 0.511
1.52ArgPhe: 1.52 ± 0.316
2.111ArgGly: 2.111 ± 0.484
0.253ArgHis: 0.253 ± 0.154
3.041ArgIle: 3.041 ± 0.548
4.392ArgLys: 4.392 ± 0.611
2.365ArgLeu: 2.365 ± 0.509
0.676ArgMet: 0.676 ± 0.212
2.449ArgAsn: 2.449 ± 0.476
0.591ArgPro: 0.591 ± 0.232
0.76ArgGln: 0.76 ± 0.261
0.76ArgArg: 0.76 ± 0.243
1.182ArgSer: 1.182 ± 0.275
2.365ArgThr: 2.365 ± 0.389
1.689ArgVal: 1.689 ± 0.323
0.253ArgTrp: 0.253 ± 0.153
1.267ArgTyr: 1.267 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
3.378SerAla: 3.378 ± 0.654
0.76SerCys: 0.76 ± 0.265
2.28SerAsp: 2.28 ± 0.458
5.405SerGlu: 5.405 ± 0.808
2.703SerPhe: 2.703 ± 0.321
3.041SerGly: 3.041 ± 0.532
0.338SerHis: 0.338 ± 0.177
4.561SerIle: 4.561 ± 0.536
7.517SerLys: 7.517 ± 0.904
4.983SerLeu: 4.983 ± 0.633
0.76SerMet: 0.76 ± 0.251
2.956SerAsn: 2.956 ± 0.482
1.351SerPro: 1.351 ± 0.372
1.943SerGln: 1.943 ± 0.365
2.28SerArg: 2.28 ± 0.488
4.307SerSer: 4.307 ± 0.625
2.618SerThr: 2.618 ± 0.612
2.534SerVal: 2.534 ± 0.357
0.76SerTrp: 0.76 ± 0.248
3.463SerTyr: 3.463 ± 0.587
0.0SerXaa: 0.0 ± 0.0
Thr
2.534ThrAla: 2.534 ± 0.419
0.338ThrCys: 0.338 ± 0.164
2.872ThrAsp: 2.872 ± 0.451
3.97ThrGlu: 3.97 ± 0.62
2.618ThrPhe: 2.618 ± 0.346
3.716ThrGly: 3.716 ± 0.517
0.507ThrHis: 0.507 ± 0.182
4.814ThrIle: 4.814 ± 0.577
6.588ThrLys: 6.588 ± 0.905
4.476ThrLeu: 4.476 ± 0.531
0.76ThrMet: 0.76 ± 0.28
2.449ThrAsn: 2.449 ± 0.419
1.943ThrPro: 1.943 ± 0.368
1.351ThrGln: 1.351 ± 0.347
1.098ThrArg: 1.098 ± 0.318
2.365ThrSer: 2.365 ± 0.408
2.534ThrThr: 2.534 ± 0.454
2.787ThrVal: 2.787 ± 0.565
0.253ThrTrp: 0.253 ± 0.129
1.858ThrTyr: 1.858 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
3.041ValAla: 3.041 ± 0.641
0.169ValCys: 0.169 ± 0.117
2.956ValAsp: 2.956 ± 0.431
4.645ValGlu: 4.645 ± 0.483
1.605ValPhe: 1.605 ± 0.349
2.28ValGly: 2.28 ± 0.449
0.422ValHis: 0.422 ± 0.161
4.645ValIle: 4.645 ± 0.502
6.841ValLys: 6.841 ± 0.76
3.97ValLeu: 3.97 ± 0.468
0.845ValMet: 0.845 ± 0.31
3.547ValAsn: 3.547 ± 0.445
1.098ValPro: 1.098 ± 0.315
1.689ValGln: 1.689 ± 0.318
2.027ValArg: 2.027 ± 0.367
3.209ValSer: 3.209 ± 0.441
2.365ValThr: 2.365 ± 0.421
2.28ValVal: 2.28 ± 0.416
0.591ValTrp: 0.591 ± 0.216
1.774ValTyr: 1.774 ± 0.416
0.0ValXaa: 0.0 ± 0.0
Trp
0.338TrpAla: 0.338 ± 0.142
0.338TrpCys: 0.338 ± 0.141
0.422TrpAsp: 0.422 ± 0.215
1.098TrpGlu: 1.098 ± 0.348
0.591TrpPhe: 0.591 ± 0.233
0.507TrpGly: 0.507 ± 0.264
0.084TrpHis: 0.084 ± 0.092
1.098TrpIle: 1.098 ± 0.26
0.929TrpLys: 0.929 ± 0.283
0.845TrpLeu: 0.845 ± 0.258
0.169TrpMet: 0.169 ± 0.09
0.76TrpAsn: 0.76 ± 0.224
0.0TrpPro: 0.0 ± 0.0
0.169TrpGln: 0.169 ± 0.127
0.169TrpArg: 0.169 ± 0.107
0.253TrpSer: 0.253 ± 0.126
0.338TrpThr: 0.338 ± 0.142
0.76TrpVal: 0.76 ± 0.177
0.0TrpTrp: 0.0 ± 0.0
0.507TrpTyr: 0.507 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.774TyrAla: 1.774 ± 0.432
0.591TyrCys: 0.591 ± 0.231
2.196TyrAsp: 2.196 ± 0.515
2.956TyrGlu: 2.956 ± 0.492
2.365TyrPhe: 2.365 ± 0.507
2.196TyrGly: 2.196 ± 0.414
0.591TyrHis: 0.591 ± 0.184
3.632TyrIle: 3.632 ± 0.569
5.068TyrLys: 5.068 ± 0.615
2.872TyrLeu: 2.872 ± 0.495
0.929TyrMet: 0.929 ± 0.303
2.534TyrAsn: 2.534 ± 0.433
0.929TyrPro: 0.929 ± 0.27
1.689TyrGln: 1.689 ± 0.41
1.436TyrArg: 1.436 ± 0.32
3.716TyrSer: 3.716 ± 0.497
2.196TyrThr: 2.196 ± 0.45
1.858TyrVal: 1.858 ± 0.32
0.76TyrTrp: 0.76 ± 0.243
1.858TyrTyr: 1.858 ± 0.383
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (11841 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski