Halorubrum sp. SD626R

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U7F6P7|A0A4U7F6P7_9EURY VWA domain-containing protein OS=Halorubrum sp. SD626R OX=1419722 GN=EXE53_14145 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 3.35IEE5 pKa = 4.32TSTVSEE11 pKa = 4.09EE12 pKa = 5.04GYY14 pKa = 10.95ACTSQVGEE22 pKa = 4.6FDD24 pKa = 3.93LQIDD28 pKa = 4.14ATDD31 pKa = 3.52EE32 pKa = 4.15TGPNPNAALVATYY45 pKa = 10.17ASCYY49 pKa = 10.27LPAFRR54 pKa = 11.84VGGSQRR60 pKa = 11.84GEE62 pKa = 3.73EE63 pKa = 4.27DD64 pKa = 3.05LGKK67 pKa = 10.15IQIDD71 pKa = 3.73ASADD75 pKa = 3.68LDD77 pKa = 4.98DD78 pKa = 6.67DD79 pKa = 5.97DD80 pKa = 6.42DD81 pKa = 4.43LASIAFDD88 pKa = 3.26VHH90 pKa = 7.85VEE92 pKa = 3.74ADD94 pKa = 4.0LDD96 pKa = 4.28DD97 pKa = 4.07GTAAEE102 pKa = 4.01IAEE105 pKa = 4.18RR106 pKa = 11.84AEE108 pKa = 5.1GICHH112 pKa = 4.59VHH114 pKa = 5.25SALRR118 pKa = 11.84EE119 pKa = 4.03GLHH122 pKa = 6.8ADD124 pKa = 3.23VSVYY128 pKa = 10.47PGAFF132 pKa = 3.1

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U7F4R8|A0A4U7F4R8_9EURY Uncharacterized protein OS=Halorubrum sp. SD626R OX=1419722 GN=EXE53_18230 PE=4 SV=1
MM1 pKa = 7.54TEE3 pKa = 3.51QPAAQVTHH11 pKa = 7.03RR12 pKa = 11.84GHH14 pKa = 6.0LRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84DD23 pKa = 2.75TRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SCGRR33 pKa = 11.84RR34 pKa = 11.84GGRR37 pKa = 11.84ARR39 pKa = 11.84SGTPCSLQLHH49 pKa = 5.75TARR52 pKa = 11.84SRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84SGLVRR62 pKa = 11.84GAGQSPTCGHH72 pKa = 5.62GRR74 pKa = 11.84GRR76 pKa = 11.84RR77 pKa = 3.48

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6274

0

6274

1603608

21

1920

255.6

27.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.916 ± 0.059

0.668 ± 0.012

8.934 ± 0.04

8.444 ± 0.041

3.213 ± 0.02

9.011 ± 0.036

1.867 ± 0.016

3.87 ± 0.025

1.73 ± 0.019

8.594 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.629 ± 0.015

2.194 ± 0.017

4.715 ± 0.02

2.112 ± 0.02

6.891 ± 0.039

5.323 ± 0.025

6.098 ± 0.027

9.199 ± 0.034

1.054 ± 0.011

2.538 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski